3’ Amino,

C7 CPG

AHP-CH-AMI0103 100 mg Reference:

AHP-CH-AMI1003 1000 mg
Nelson PS, Sherman-Gold R, Leon R. A new and versatile reagent
Name: for incorporating multiple primary aliphatic amines into synthetic
Chemical Name: 1-Dimethoxytrityloxy-3-[O-(N- oligonucleotides. Nucleic Acids Res., 17, 7179-86. (1989)

chain alkylamino-CPG Nelson PS, Kent M, Muthini S. Oligonucleotide labeling methods. III:
Formula: N/A Direct labeling of oligonucleotides employing a novel, non-nucleosidic,
2-aminobutyl-1,3-propanediol backbone. Nucleic Acids Res., 20, 6253-
6259. (1992)

Lyttle MH, Adams H, Hudson D, Cook RM. Versatile Linker Chemistry

(1997)

Petrie CR, Reed MW, Adams AD, and Meyer RB. An improved
CPG support for the synthesis of 3’-amine-tailed oligonucleotides.
Bioconjugate Chemistry, 3, 85-7. (1992)

Use: 3’-FAM CPG is used to label amino at the 3’ end of

oligonucleotides.
Purity: 95%+
Diluent: N/A, Stability in Solution: N/A

Background:

amino group into the oligo. A primary amino group can be

used to attach an oligonucleotide to a solid surface. Users

may print their own oligonucleotide-based microarrays using

hydrophobic. The available lengths are 3, 6, or 12 methylene

(CH2) groups between the terminal phosphate and the amino
“moiety”.

be introduced to oligonucleotide sequences using Amino-

Coupling: Proceed in a manner identical to the use of normal

nucleoside support since it contains the DMT group.

Deprotection: Use ammonium hydroxide to cleave the

oligonucleotide. This requires 2 hours at room temperature.
Use an appropriate protocol (depending on the nucleobases) to
complete the deprotection.

Storage: Freezer storage, -10 to -30°C, dry, dark area

Labeled Oligo Storage: Store labeled oligo in the dark, either

dry or in a neutral aqueous media at -20°C. Do not store crude

MSDS: www.allelebiotech.com