Protein Estimation by Lowry’s Method

Solution A: 25ml
1.0.5g Na2 CO3
2.0.1N NaOH (0.1g NaOH)
0.5ml of Solution B

Solution B: 25ml
0.5% CuSo4 (0.125g)
0.1% K Na C4 H4 O6. 4H2O
(Sodium Potassium Tartarate)

BSA Stock: 10mg/ml

Working Solution: 100ul of Stock in 900ul H2O – Final conc is 1ug/ul

FC Reagent: 1:1 in dd H2O

FC
  BSA  H20 AlCuSO4  Reagent
  Standard (ul) (ul) (ul) Incubate the
  (ug)       tubes in dark for
1 10 190 1000 100 30’min and
protein is
2 20 180 1000 100
measured at
3 30 170 1000 100
680nm
4 40 160 1000 100
5 50 150 1000 100