BIOREACTOR

P.V. S. YUGANDHAR M.Tech

(Biotech)
BIOREACTOR
 A bioreactor is a reactor system used for
the culture of LIVING CELLS. They vary
in size and complexity from a 10 ml
volume in a test tube to computer
controlled fermenters with liquid
volumes greater than 100 m3. They
similarly vary in cost from a few cents to
a few million dollars.

P.V. S. YUGANDHAR M.Tech

(Biotech)
The main function of a bioreactor is
to provide a controlled environment
for the growth of living cells and in
the process, obtain a desired product

P.V. S. YUGANDHAR M.Tech

(Biotech)
P.V. S. YUGANDHAR M.Tech
(Biotech)
Design of a Bioreactor involves
co-operation between experts in :
Microbiology,Biochemistry,Chemi
cal Engineering,Mechanical
Engineering and costing

P.V. S. YUGANDHAR M.Tech

(Biotech)
Living cells
 Microbial---Bacteria, Fungi
etc.

 Plant

 Animal
P.V. S. YUGANDHAR M.Tech
(Biotech)
Designing and Construction
 Vessel should be capable of
being operated aseptically and
reliable for long-term operation----
meet requirements of
containment regulation
 Adequate aeration and agitation
 Mixing should not damage the
organism
P.V. S. YUGANDHAR M.Tech
(Biotech)
Designing and Construction
 Power consumption should be as
low as possible
 System of temperature control
 System of pH control
 Sampling facilities provided
 Evaporation loss from bioreactor
should not be excessive

P.V. S. YUGANDHAR M.Tech

(Biotech)
Designing and Construction
 Vessel should be constructed to
ensure smooth internal
surfaces,using welds instead of
flange joints wherever possible
 Vessel should be of similar
geometry to both smaller and
larger vessels, to facilitate scale-
up

P.V. S. YUGANDHAR M.Tech

(Biotech)
Designing and Construction
 Cheapest materials which
enable satisfactory results to
be used
 There should be adequate
service provisions

P.V. S. YUGANDHAR M.Tech

(Biotech)
P.V. S. YUGANDHAR M.Tech
(Biotech)
P.V. S. YUGANDHAR M.Tech
(Biotech)
SUMMARY
 Main function of a Bioreactor is the
provision of controlled environment
for growth of living cells
 The design of a bioreactor requires a
multi-disciplinary approach
 The various salient points to be
considered for designing and
construction of a bioreactor were
discussed
P.V. S. YUGANDHAR M.Tech
(Biotech)
GOOD LUCK !

P.V. S. YUGANDHAR M.Tech

(Biotech)