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By' Day_e Sanders

ThemieTwo Malking olf an Antibody

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Understanding some characteristics of a target audience:

• Socio-cultural variety (not just one person)

• The audience's degree of knowledge on subject

• What will the audience see/learn/conclude?

• Challenge all assumptions/look for a quirk that will catch attention

• To incubate great ideas, your mind needs [scientific] facts to work with",

Initially, my target audience for this unit was ages 13-18 - high school education - however I feel that it may appeal on a broader scope, Science is interesting, and when people "really'" take the time to stt down and indulge they'll be surprised at how engaging the experience is; regardless of how small or big the discovery is.

I aim to make my animation simple yet effective to achieve this niche ..

Thinking about how I can recreate appearances whilst maintaining the process. Inspired by visual. "feeds", such as the growth of the. antibody and bioluminescence to signify this growth. I want to keep an eye on the core functions of what I create.

INlarrative Structure

ACT 1

Camera will rush through the blood vessel, audience will be in the pulmonary circulation. Passing blood cells will flow in the current and bounce lightly off of the vessel's elastic wall. Opening credits will play out and on screen text will blend in to indicate what is happening in the animation throughout.

A key feature of the immune system is the ability to distinguish between endogenous and exogenous structures (cells inside and outside the body) that are not dangerous.The bodies humoral, or antibody-mediated immune response begins in the same manner as its cell-mediated response.

INlarrative Structure

ACTZ

Camera zooms in on a b-lvrnphocvte (white blood cell (comes from bone marrow)). The b-lvmphocvte contains many proteins on its surface: These are membrane bound antibodies/immunoglobulins .. These look a lot look like underwater anemones and genus', or even small wriggly caterpillars,

The pathogen will find a suitable binding site, locking itself into the membrane bound antibody. It binds with its epitope,and it is only compatible with a particular antibody and one of it's variable portions. On an antibody, there are two binding sites at the end of each tip of the "V" between the variable regions of the light and heavy chains.

Triggered by this meeting, the helpers release chemicals which spur the selected b-cells into rapid reproduction. Some b-cells become memory cells ready to respond to a later invasion by the same pathogen, but most become antibody producing factories called plasma cells.

The generated antibodies (antibody "offspring" from plasma cell factories) will take appearance of butterflies, but this is aU metaphorical. Based on the evolution process ofthe membrane bound "caterpillar" antibodies becoming "butterfly" antibodies - where they'll remain in the fluids of the immune system for a while.

INlarrative Structure

ACT 3

Pathogens will continue to infect the immune system, but the antibodies will be going to continuously reproduce. They remember the pathogenic interaction. They then flow around the vein to chase after similar remaining pathogens. They do this to maintain a systematic arrangement for a better, overall immune protection.

The newborn antibodies (detached and floating in the fluid (the second type of antibody which is not surface bound)) will begin the tagging process once they locate the remaining fragments of the pathogen: This process is called "opsonisation".

This unique immune signal alerts one of the many macrophages. When one is alerted, it promptly arrives on the scene to disable the pathogen: The macrophage on scene will prepare Itself for phagocytosis. This interaction involves engulfing the memory cells and the pathogens, imprisoning the pathogen for "interrogation".

The macrophage will present a piece of that consumed antibody on an MHC Class-Z molecule. This molecule will be sent a to aT-helper (CD-4) cell to activate more macrophages and also B-cell maturation and proliferation for antibody production.