Golgi Staining Protocol for Neurons and Processes

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Golgi Staining Method and Protocol for Neurons and Processes

 
NovaUltra Special Stain Kits
 
Solutions and Reagents:
Fixative Solution:
 
      3% potassium bichromate  ---------- 60 ml
      10% formalin -------------------------- 20 ml
      Mix well and store at room temperature.
 
2% Silver Nitrate Solution:
 
      Silver nitrate ---------------------------- 2 g
      Distilled water -------------------------- 100 ml
      Mix well and store at room temperature.
 
Procedure:
 
1. Fix tissue blocks (less than 1 cm thick) in 10% formalin for 24 hours or more. The
alternatively you can also use the above fixative solution:
2. Place tissue blocks in 3% potassium bichromate for 3-7 days. Avoid light. Change fresh
solution each day.
 
3. Transfer tissue blocks into 2% silver nitrate solution for 1-3 day at room temperature. Avoid
light. (Note: before put blocks into silver nitrate solution, use filter paper to absorb access
solution. Change silver nitrate solution (less than regular use) several times until brown
participates do not appear).
 
4. Cut vibratome sections at 60 um thick into distilled water.
 
5. Mount sections on to Superfrost Plus slides. Air dry for 10 minutes. Dehydrate through 95%
Alcohol, 100% alcohol, clear in xylene and coverslip.
Results:
 
Cortical neurons and processes ----------- black
     
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      Brain (Cortex).
   

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Golgi Staining Protocol for Neurons and Processes

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