Fluorescence spectroscopy

Lecture # 1
 Objectives
After this lecture students will be able to
• Define Fluorescence spectroscopy
• Explain different steps of Fluorescence
 Fluorescence spectroscopy
It is is a type of electromagnetic spectroscopy
which analyzes fluorescence from a sample. It
uses usually ultraviolet light, that excites the
electrons in molecules of certain compounds
and causes them to emit light of a lower
energy
Phases of Fluorescence spectroscopy
• Fluorescence
• Vibrational Relaxation
• Phosphorescence
Fluorescence spectroscopy

Lecture # 2
Quantum Efficiency Of Fluorescence
spectroscopy
The ratio of the number of photon emitted and
the number of photon absorbed .It is also
called quantum yield of florescence intensity
and denoted by ф.
 Factors Effecting Florescence
Concentration
Quantum yield
Intensity of incident light
Pathlength
Adsorption
 Factors Effecting Florescence
Oxygen
pH
Photochemical decomposition
Temperature and viscosity
Quenchers
Scatter