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    Protocol 4% PAF

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    Mandar Bawadekar
    Paraformaldehyde is a toxic but rapid-fixing chemical. To make a 4% paraformaldehyde solution, carefully add 4g of paraformaldehyde to 100ml of phosphate buffered saline in a fume hood. Warm the cloudy mixture on a hotplate with stirring until it clears, then allow it to cool while continuing to stir before refrigerating for storage. Proper safety precautions are required when handling paraformaldehyde as it can harm the body upon contact.

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    Protocol 4% PAF

    Uploaded by

    Mandar Bawadekar
    237 views1 page
    Paraformaldehyde is a toxic but rapid-fixing chemical. To make a 4% paraformaldehyde solution, carefully add 4g of paraformaldehyde to 100ml of phosphate buffered saline in a fume hood. Warm the cloudy mixture on a hotplate with stirring until it clears, then allow it to cool while continuing to stir before refrigerating for storage. Proper safety precautions are required when handling paraformaldehyde as it can harm the body upon contact.

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    Paraformaldehyde is a toxic but rapid-fixing chemical. To make a 4% paraformaldehyde solution, carefully add 4g of paraformaldehyde to 100ml of phosphate buffered saline in a fume hood. Warm the cloudy mixture on a hotplate with stirring until it clears, then allow it to cool while continuing to stir before refrigerating for storage. Proper safety precautions are required when handling paraformaldehyde as it can harm the body upon contact.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as doc, pdf, or txt
    237 views1 page

    Protocol 4% PAF

    Uploaded by

    Mandar Bawadekar
    Paraformaldehyde is a toxic but rapid-fixing chemical. To make a 4% paraformaldehyde solution, carefully add 4g of paraformaldehyde to 100ml of phosphate buffered saline in a fume hood. Warm the cloudy mixture on a hotplate with stirring until it clears, then allow it to cool while continuing to stir before refrigerating for storage. Proper safety precautions are required when handling paraformaldehyde as it can harm the body upon contact.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
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    of 1

    4% PARAFORMALDEHYDE/PBS

    1. Measure 100ml Phosphate Buffered Saline (PBS) into a measuring cylinder.


    Pour into the conical flask containing 4g of paraformaldehyde. Cover with
    parafilm and transfer to the fume hood: thoroughly shake - take care not to splash
    paraformaldehyde about - it is a rapid fixer and is TOXIC.

    2. Place flask on top of the hotplate/stirrer inside the fume cupboard and set the
    heat control to 7 with moderate stirring. Allow the solution to warm up-it will turn
    from being cloudy to clear when ready. Inspect regularly to avoid over- heating
    and consequent spilling.

    3. When the paraformaldehyde has dissolved, switch off the heat but leave to stir:
    do not handle for safety reasons. Allow to cool.

    4. When cooled, transfer the fixative to a 4C refrigerator. Label appropriately and


    date.

    Safety:

    Paraformaldehyde is a rapid fixative and will cause harm if it comes into contact
    with the body

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