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  • Grafik Percobaan Protein

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    Ratna Apriyani
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    The document contains two calibration curves comparing the concentration of a standard in parts per million (ppm) to its measured absorbance. The first is for the Biuret method and shows a linear relationship between 0-180 ppm with an R2 value of 0.974. The second is for the Lowry method and also shows a linear relationship between 0-33.3 ppm with an R2 value of 0.960. Both methods demonstrate a direct proportional relationship between concentration and absorbance that can be used to determine unknown concentrations.

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    The document contains two calibration curves comparing the concentration of a standard in parts per million (ppm) to its measured absorbance. The first is for the Biuret method and shows a linear relationship between 0-180 ppm with an R2 value of 0.974. The second is for the Lowry method and also shows a linear relationship between 0-33.3 ppm with an R2 value of 0.960. Both methods demonstrate a direct proportional relationship between concentration and absorbance that can be used to determine unknown concentrations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as xlsx, pdf, or txt
    6 views1 page

    Grafik Percobaan Protein

    Uploaded by

    Ratna Apriyani
    The document contains two calibration curves comparing the concentration of a standard in parts per million (ppm) to its measured absorbance. The first is for the Biuret method and shows a linear relationship between 0-180 ppm with an R2 value of 0.974. The second is for the Lowry method and also shows a linear relationship between 0-33.3 ppm with an R2 value of 0.960. Both methods demonstrate a direct proportional relationship between concentration and absorbance that can be used to determine unknown concentrations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as xlsx, pdf, or txt
    of 1

    Metode Biuret

    Konsentrasi(ppm) absorbansi
    0 0 KURVA KALIBRASI [STANDAR] Vs
    20 0.003 ABSORBANSI
    60 0.02 0.05
    100 0.022
    0.04
    140 0.025 f(x) = 0.000214545454545 x

    Absorbansi
    0.03 R² = 0.974330864773456
    180 0.04
    0.02
    0.01
    0
    0 20 40 60 80 100 120 140 160 180 200
    [STANDAR] ppm

    Metode Lowry
    Konsentrasi (ppm) absorbansi
    0 0 KURVA KALIBRASI [STANDAR] Vs
    6.67 0.046 ABSORBANSI
    13.3 0.154
    0.25
    20 0.114
    0.2 f(x) = 0.006873404754803 x
    26.7 0.166 R² = 0.960201058453314
    33.3 0.232
    Absorbansi

    0.15

    0.1

    0.05

    0
    0 5 10 15 20 25 30 35
    [STANDAR] ppm

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