MICROPROPAGATION

POPULARLY KNOWN FOR LARGE SCALE CLONAL PROPAGATION - IS THR FIRST MAJOR AND WIDELY ACCEPTED PRACTICAL APPLICATION OF PLANT BIOTECHNOLOGY. NOW IT HAS GAINED STATUS OF MULTI-BILLION DOLLAR INDUSTRY BEING PRACTICED IN MANY BIOTECHNOLOGY LABS AND NURSERIES

APPLICATIONS


 

APPLICABLE TO ORNAMENTAL PLANTS, FRUITS,VEGETABLES,TREES,MEDICINAL AND AROMATIC PLANTS USED FOR THOSE PLANTS WHICH ARE DIFFICULT TO PROPAGATE BY SEEDS OR VEGETATIVE MEANS MASS MULTIPLICATION OF EXISTING STOCKS OF GERMPLASM FOR CONSERVATION OF IMPORTANT, ELITE AND RARE PLANT SPECIES BIOMASS ENERGY PRODUCTION HAS BEEN APPLIED TO MORE THAN ONE THOUSAND SPECIES

FACTORS
  

EXPLANT SOURCE- TYPE, NATURE,SIZE MEDIUM COMPOSITION AND PLANT GROWTH HORMONES PHYSICAL CONDITIONS INTENSITY AND QUALITY OF LIGHT PHOTOPERIOD TEMPERATURE RELATIVE HUMIDITY CULTURE VESSEL TYPE HARDENING AND ACCLIMATIZATION

MERITS


MICROPROPAGATED PLANTS ARE GENETICALLY HOMOGENOUS SHOOT MERISTEM CULTURED DERIVED PLANTS ARE VIRUS FREE PLANTS CAN BE PRODUCED INDEPENDENTLY OF SEASON AND OTHER ENVIRONMENTAL CONDITIONS SOMATIC EMBRYOGENESIS ALLOWS HIGH PRODUCTION EFFICIENCY LARGE NO. OF ELITE PLANTS CAN BE MAINTAINED IN A SMALL SPACE AND FOR A LONG TIME CONSERVATION OF IMPORTANT, RARE AND ENDEMIC PLANT SPECIES

DEMERITS


HIGHER COST OF PLANT PRODUCTION HAS LIMITED THE USE AND APPLICATION OF THE TECHNIQUE

COST REDUCTION STRATEGY



USE OF SHAKE CULTURES UTILIZING LIQUID CULTURE MEDIUM



CLOSE CONTACT OF THE TISSUE WITH THE MEDIUM STIMULATE AND FACILITATE THE UPTAKE OF NUTRIENTS AND PHYTOHORMONES LEADING TO BETTER SHOOT AND ROOT GROWTH DISAPPEARANCE OF LESSER EXPRESSION OF ACTIVITY OF APICAL DOMINANCE DUE TO CONTINUOUS SHAKING CONDITIONS LEADS TO INDUCTION AND PROLIFERATIONOF NUMEROUS AXILLARY BUDS SHAKING OF THE MEDIUM PROVIDES SUFFICIENT OXYGEN SUPPLY ULTIMATELY LEADING TO FASTER GROWTH

LARGE SCALE CULTURE OF PLANTS IN BIOREACTORS



TO REDUCE THE COST OF PLANT PRODUCTION THERE IS NEED TO DEVELOP SCALED UP SYSTEMS AND AUTOMATION POTENTIAL FOR SCALING UP IN LESSER TIME LIMIT, REDUCTION IN PRODUCTION COST;AUTOMATED CONTROL OF PHYSICAL AND CHEMICAL ENVIRONMENT DURING GROWTH PHASE CULTUREBAL UNITS- MERISTEM TIPS; BULBLETS; CORMS; MICROTUBERS; NODES DIFFERENT DESIGNS AND TYPES OF BIOREACTORS HAVE BEEN EMPLOYED

GLYCYRRHIZA GLABRA [LICORICE]

AS MEDICINE IN PHARMACEUTICAL INDUSTRY COUGH SYRUPS AND THROAT LOZENGES AS EXPECTORANT, ANTITUSSIVE ULCER THERAPY- GASTRIC AND DUODENAL ULCER COFECTIONARY INDUSTRY FLAVOURING AGENT AND AS SWEETNER IN LOW CALORIE FOODS; CHOCOLATES; CANDIES; BAKED GOODS; BEERS; DIABETIC FOODS

HAIRY ROOTS-GENETICALLY TRANSFORMED ROOTS



DEVELOP IN RESPONSE TO A SOIL DWELLING BACTERIUM AGROBACTERIUM RHIZOGENES INFECTION FROM THE SITES OF INFECTION CAUSING NEOPLASTIC DISEASE SYNDROME HAIRY ROOT DISEASE

CHRACTERISTICS OF HAIRY ROOTS

   

RAPID GROWTH SECONDARY METABOLITE PRODUCTION GENETIC STABILITY MORPHOGENETIC POTENTIAL

GROWTH OF HAIRY ROOT CLONE G6 IN SHAKE FLASK AND BIOREACTOR

ROOTS

CULTURE VESSEL FLASK 250 ML

CULTURE PERIOD 30 DAYS

VOLUME OF MEDIUM 50 ML

INITIAL F.WT(G) 0.18

FINAL F.WT(G) 0.30

FOLD INCREASE 1.7

NORMAL ROOTS

HAIRY FLASK ROOT 250 ML CLONE G6 HAIRY BIOREAC ROOT TOR 5 L CLONE G6

30 DAYS

50 ML

0.18

3.40

15

30 DAYS

4L

16.0

310

20