Charm ii Antibiotic Analysis for Honey

proCeDural FloWCHart

Binding Reagent Tablet

Tracer Tablet

+
Sample

Charm ii 6600 analyzer

Incubate

Families DeteCteD

= = = = = = =

Aminoglycosides Amphenicols/Chloramphenicol Beta-lactams Macrolides (Tylosin) Nitrofurans Sulfonamides Tetracyclines

Centrifuge Resuspend

C2Soft
Count Results
(optional)

sample siZe sample preparation

5 g / 10 g / 20 g Minimal sample preparation needed. Simple dilution of honey with supplied extraction buffer. For some drugs, solid-phase extraction is required. Approximately 10 minutes, depending on the number of samples. 6 to 12 samples in assay, depending on drug family.

Computer Report

sample printout Date = 08/23/10 Time = 14:28:12 Operator Time Counted Sample I.D. = 1 = 60 = 1258

assaY time

CapaCitY

Assay = Chloramphenicol Lot# = ATBL 005 = 1190 = 1450 Control Point Sample (CPM)

Interpretation = Not Found

Charm sciences, inc. 659 Andover Street, Lawrence, MA 01843, USA | Tel: +1.978.687.9200 | www.charm.com

Charm ii Antibiotic Analysis for Honey

Charm ii Kit aminoglycosides (STIIHH) amphenicols (CIIHH) Beta-lactams (PIIH) Drug
Streptomycin Dihydrostreptomycin Chloramphenicol Florfenicol Thiamphenicol Penicillin-G Amoxicillin Ampicillin Cephapirin Ceftiofur Cloxacillin Oxacillin Dicloxacillin Chloramphenicol Erythromycin Spiramycin Pirlimycin Tilmicosin Lincomycin AOZ AMOZ Sulfamethazine Sulfadimethoxine Sulfamerazine Sulfathiazole Sulfachlorpyridazine Sulfamethoxypyridine Sulfapyridine Sulfisoxazole Sulfamethoxazole Dapsone Sulfadoxine Sulfapyridazine Tetracycline Chlortetracycline Oxytetracycline

test sensitivity1 (ppb)

5 10 10 UD UD 12.5 25 25 10 25 100 80 80 0.3 10 - 15 15 - 20 30 - 40 20 - 30 20 - 30 <0.3 <0.3 10 5 20 20 10 5 15 20 20 5 20 10 15 10 20

Chloramphenicol (ATBLIIHH) macrolides (MIIH)

nitrofurans (AOZIIH) (AMOZIIH) sulfonamides (SMIIHH)

tetracyclines (TETH)

Exceeds 90% positive at a 95% confidence limit UD=Undetermined

1

Charm sciences, inc.

659 Andover Street, Lawrence, MA 01843-1032, USA Tel: +1.978.687.9200 | Fax: +1.978.687.9216 | Email: info@charm.com | www.charm.com
Copyright 2010 Charm Sciences, Inc. Charm is a registered trademark of Charm Sciences, Inc. MRK-122-001 0810