Graded-Dose Response Curve SECTION A

Paracetamol Foot Volume

Preparation: 500 mg tablet Before Carageenan 1 hr 2 hrs
Doses: 0.5 mg / 200 gm rat
Rat 1 0 0 0.01 0
Rat 2 0 0 0.01 0.01
Rat 3 0 0 0.01 0.01
Rat 4 0 0 0 0
Rat 5 0 0 0.01 0
Rat 6 0 0 0.01 0
Rat 7 0 0 0 0.01
Rat 8 0 0.01 0.01 0

1.6 mg / 200 gm rat

Rat 1 0 0 0.01 0
Rat 2 0 0.01 0.01 0
Rat 3 0 0 0.01 0.01
Rat 4 0 0 0 0.01
Rat 5 0 0 0.01 0.01
Rat 6 0 0 0 0.01
Rat 7 0 0 0.01 0
Rat 8 0 0 0.01 0.01

5 mg / 200 gm rat Rat 1 0 0 0 0

Rat 2 0 0 0 0
Rat 3 0 0 0.01 0.01
Rat 4 0 0.01 0.01 0
Rat 5 0 0 0 0
Rat 6 0 0 0 0
Rat 7 0 0.01 0.01 0.01
Rat 8 0 0 0 0

16 mg / 200 gm rat Rat 1 0 0 0 0.01

Rat 2 0 0 0.01 0
Rat 3 0 0 0 0.01
Rat 4 0 0 0.01 0
Rat 5 0 0 0 0.01
Rat 6 0 0 0.01 0.01
Rat 7 0 0 0.01 0.01
Rat 8 0 0 0.01 0
ON A

3 hrs

0
0.01
0.01
0.01
0.01
0.01
0.01
0

0.01
0
0.01
0
0.01
0.01
0
0.01

0
0.01
0.01
0.01
0.01
0
0
0.01

0.01
0.01
0.01
0.01
0.01
0
0
0.01
 Graded-Dose Response Curve SECTION B
Aspirin Foot Volume
Preparation: 200 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.5 mg / 200 gm rat
Rat 1 0 0 0 0 0
Rat 2 0 0 0.01 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0 0 0 0
Rat 6 0 0 0.01 0 0
Rat 7 0 0.01 0 0 0
Rat 8 0 0 0 0 0

1.6 mg / 200 gm rat

Rat 1 0 0 0 0 0
Rat 2 0 0 0 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0.01 0 0 0
Rat 6 0 0.01 0 0 0
Rat 7 0 0 0 0 0
Rat 8 0 0.01 0 0 0

5 mg / 200 gm rat Rat 1 0 0 0 0 0

Rat 2 0 0 0 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0 0.01 0.01 0
Rat 6 0.01 0 0 0 0
Rat 7 0.01 0.01 0.01 0 0
Rat 8 0 0.01 0 0 0

16 mg / 200 gm rat Rat 1 0 0 0 0 0

Rat 2 0 0 0.01 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0 0 0 0
Rat 6 0 0 0 0 0.01
Rat 7 0 0 0 0 0
Rat 8 0 0 0 0 0.01
 Graded-Dose Response Curve SECTION C
Celecoxib Foot Volume
Preparation:200 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.3 mg / 200 gm rat
Rat 1 0 0 0.01 0 0
Rat 2 0 - - - -
Rat 3 0 0.01 0.01 0 0.01
Rat 4 0 0 0 0 0
Rat 5 0 0.01 0 0.01 0.01
Rat 6 0 0 0 0 0
Rat 7 0 0 0 0 0
Rat 8 0 0.01 0.01 0 0

1 mg / 200 gm rat
Rat 1 0 0.01 0 0 0.01
Rat 2 0 0 0 0 0
Rat 3 0.01 0 0 0.01 0
Rat 4 0 0.01 0.01 0 0.01
Rat 5 0 0.01 0.01 0 0
Rat 6 0.01 0.01 0 0.01 0.01
Rat 7 0 0.01 0 0 0
Rat 8 0 0.01 0 0 0

3 mg / 200 gm rat Rat 1 0 0.01 0 0 0

Rat 2 0.01 0.01 0.01 0 0
Rat 3 0 0.01 0.01 0 0
Rat 4 0.01 0.01 0 0 0
Rat 5 0 0.01 0 0 0
Rat 6 0 0.01 0 0 0
Rat 7 0.01 0.01 0.01 0 0
Rat 8 0.01 0 0 0 0

10 mg / 200 gm rat Rat 1 0 0.01 0.01 0.01 0

Rat 2 0 0.01 0.01 0 0
Rat 3 0.01 0.01 0.01 0 0
Rat 4 0.01 0.01 0.01 0 0.01
Rat 5 0.01 0 0.01 0 0
Rat 6 0.01 0.01 0 0 0
Rat 7 0.01 0.01 0.01 0 0
Rat 8 0.01 0.01 0 0 0.01
 Graded-Dose Response Curve SECTION D
Prednisone Foot Volume
Preparation: 20 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.02 mg / 200 gm rat
Rat 1 0 0 0.01 0 0.01
Rat 2 0 0.01 0.01 0 0
Rat 3 0 0 0.01 0 0.01
Rat 4 0 0.01 0.01 0 0
Rat 5 0 0 0.01 0 0.01
Rat 6 0 0 0.01 0 0
Rat 7 0 0 0.01 0 0
Rat 8 0 0.01 0 0 0

0.06 mg / 200 gm rat

Rat 1 0 0 0.01 0 0.01
Rat 2 0 0.01 0.01 0 0
Rat 3 0 0 0 0 0.01
Rat 4 0 0.01 0 0 0
Rat 5 0 0.01 0.01 0 0.01
Rat 6 0 0.01 0 0.01 0
Rat 7 0 0 0.01 0.01 0
Rat 8 0 0 0.01 0.01 0

0.2 mg / 200 gm rat Rat 1 0 0 0 0.01 0.01

Rat 2 0 0 0 0.01 0
Rat 3 0 0 0.01 0 0
Rat 4 0 0 0.01 0 0
Rat 5 0 0.01 0 0 0
Rat 6 0 0 0.01 0 0.01
Rat 7 0 0.01 0.01 0 0
Rat 8 0 0 0.01 0 0

0.6 mg / 200 gm rat Rat 1 0 0.01 0 0.01 0.01

Rat 2 0 0 0.01 0.01 0
Rat 3 0 0 0.01 0 0
Rat 4 0 0 0 0.01 0.01
Rat 5 0 0.01 0.01 0.01 0
Rat 6 0 0.01 0.01 0.01 0.01
Rat 7 0 0 0.01 0 0
Rat 8 0 0 0.01 0 0
 Graded-Dose Response Curve SECTION A
Paracetamol Foot Volume
Preparation:500 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.5 mg / 200 gm rat
Rat 1 0 0 0.01 0 0
Rat 2 0 0 0.01 0.01 0.01
Rat 3 0 0 0.01 0.01 0.01
Rat 4 0 0 0 0 0.01
Rat 5 0 0 0.01 0 0.01
Rat 6 0 0 0.01 0 0.01
Rat 7 0 0 0 0.01 0.01
Rat 8 0 0.01 0.01 0 0
Mean 0 0 0.01 0 0.01
Difference 0 0 0 0
% Difference 80 173.3 126.7 180.0

1.6 mg / 200 gm rat

Rat 1 0 0 0.01 0 0.01
Rat 2 0 0.01 0.01 0 0
Rat 3 0 0 0.01 0.01 0.01
Rat 4 0 0 0 0.01 0
Rat 5 0 0 0.01 0.01 0.01
Rat 6 0 0 0 0.01 0.01
Rat 7 0 0 0.01 0 0
Rat 8 0 0 0.01 0.01 0.01
Mean 0 0 0.01 0 0.01
Difference 0 0 0 0
% Difference 100 192.9 142.9 192.9

5 mg / 200 gm rat Rat 1 0 0 0 0 0

Rat 2 0 0 0 0 0.01
Rat 3 0 0 0.01 0.01 0.01
Rat 4 0 0.01 0.01 0 0.01
Rat 5 0 0 0 0 0.01
Rat 6 0 0 0 0 0
Rat 7 0 0.01 0.01 0.01 0
Rat 8 0 0 0 0 0.01
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 188.9 255.6 255.6 322.2

16 mg / 200 gm rat Rat 1 0 0 0 0.01 0.01

Rat 2 0 0 0.01 0 0.01
Rat 3 0 0 0 0.01 0.01
Rat 4 0 0 0.01 0 0.01
Rat 5 0 0 0 0.01 0.01
Rat 6 0 0 0.01 0.01 0
Rat 7 0 0 0.01 0.01 0
Rat 8 0 0 0.01 0 0.01
Mean 0 0 0 0.01 0
Difference 0 0 0 0
% Difference 110.0 280.0 320.0 290.0
 Graded-Dose Response Curve SECTION B
Aspirin Foot Volume
Preparation:200 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.5 mg / 200 gm rat
Rat 1 0 0 0 0 0
Rat 2 0 0 0.01 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0 0 0 0
Rat 6 0 0 0.01 0 0
Rat 7 0 0.01 0 0 0
Rat 8 0 0 0 0 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 38.5 84.6 -38.5 23.1

1.6 mg / 200 gm rat

Rat 1 0 0 0 0 0
Rat 2 0 0 0 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0.01 0 0 0
Rat 6 0 0.01 0 0 0
Rat 7 0 0 0 0 0
Rat 8 0 0.01 0 0 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 29.2 -20.8 -20.8 -33.3

5 mg / 200 gm rat Rat 1 0 0 0 0 0

Rat 2 0 0 0 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0 0.01 0.01 0
Rat 6 0.01 0 0 0 0
Rat 7 0.01 0.01 0.01 0 0
Rat 8 0 0.01 0 0 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 7.4 -14.8 -22.2 -48.1

16 mg / 200 gm rat Rat 1 0 0 0 0 0

Rat 2 0 0 0.01 0 0
Rat 3 0 0 0 0 0
Rat 4 0 0 0 0 0
Rat 5 0 0 0 0 0
Rat 6 0 0 0 0 0.01
Rat 7 0 0 0 0 0
Rat 8 0 0 0 0 0.01
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference -33.3 13.3 6.7 33.3
 Graded-Dose Response Curve SECTION C
Celecoxib Foot Volume
Preparation:200 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.3 mg / 200 gm rat
Rat 1 0 0 0.01 0 0
Rat 2 0
Rat 3 0 0.01 0.01 0 0.01
Rat 4 0 0 0 0 0
Rat 5 0 0.01 0 0.01 0.01
Rat 6 0 0 0 0 0
Rat 7 0 0 0 0 0
Rat 8 0 0.01 0.01 0 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 115.4 130.8 76.9 84.6

1 mg / 200 gm rat
Rat 1 0 0.01 0 0 0.01
Rat 2 0 0 0 0 0
Rat 3 0.01 0 0 0.01 0
Rat 4 0 0.01 0.01 0 0.01
Rat 5 0 0.01 0.01 0 0
Rat 6 0.01 0.01 0 0.01 0.01
Rat 7 0 0.01 0 0 0
Rat 8 0 0.01 0 0 0
Mean 0 0.01 0 0 0
Difference 0 0 0 0
% Difference 55.2 3.4 -34.5 3.4

3 mg / 200 gm rat Rat 1 0 0.01 0 0 0

Rat 2 0.01 0.01 0.01 0 0
Rat 3 0 0.01 0.01 0 0
Rat 4 0.01 0.01 0 0 0
Rat 5 0 0.01 0 0 0
Rat 6 0 0.01 0 0 0
Rat 7 0.01 0.01 0.01 0 0
Rat 8 0.01 0 0 0 0
Mean 0 0.01 0 0 0
Difference 0 0 0 0
% Difference 51.6 16.1 -12.9 -41.9

10 mg / 200 gm rat Rat 1 0 0.01 0.01 0.01 0

Rat 2 0 0.01 0.01 0 0
Rat 3 0.01 0.01 0.01 0 0
Rat 4 0.01 0.01 0.01 0 0.01
Rat 5 0.01 0 0.01 0 0
Rat 6 0.01 0.01 0 0 0
Rat 7 0.01 0.01 0.01 0 0
Rat 8 0.01 0.01 0 0 0.01
Mean 0.01 0.01 0 0 0
Difference 0 0 0 0
 % Difference 4.9 -7.3 -39.0 -31.7

Graded-Dose Response Curve SECTION D

Prednisone Foot Volume
Preparation:20 mg tablet Before Carageenan 1 hr 2 hrs 3 hrs
Doses: 0.02 mg / 200 gm rat
Rat 1 0 0 0.01 0 0.01
Rat 2 0 0.01 0.01 0 0
Rat 3 0 0 0.01 0 0.01
Rat 4 0 0.01 0.01 0 0
Rat 5 0 0 0.01 0 0.01
Rat 6 0 0 0.01 0 0
Rat 7 0 0 0.01 0 0
Rat 8 0 0.01 0 0 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 38.1 90.5 -4.8 23.8

0.06 mg / 200 gm rat

Rat 1 0 0 0.01 0 0.01
Rat 2 0 0.01 0.01 0 0
Rat 3 0 0 0 0 0.01
Rat 4 0 0.01 0 0 0
Rat 5 0 0.01 0.01 0 0.01
Rat 6 0 0.01 0 0.01 0
Rat 7 0 0 0.01 0.01 0
Rat 8 0 0 0.01 0.01 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 82.4 105.9 70.6 76.5

0.2 mg / 200 gm rat Rat 1 0 0 0 0.01 0.01

Rat 2 0 0 0 0.01 0
Rat 3 0 0 0.01 0 0
Rat 4 0 0 0.01 0 0
Rat 5 0 0.01 0 0 0
Rat 6 0 0 0.01 0 0.01
Rat 7 0 0.01 0.01 0 0
Rat 8 0 0 0.01 0 0
Mean 0 0 0 0 0
Difference 0 0 0 0
% Difference 93.8 93.8 112.5 62.5

0.6 mg / 200 gm rat Rat 1 0 0.01 0 0.01 0.01

Rat 2 0 0 0.01 0.01 0
Rat 3 0 0 0.01 0 0
Rat 4 0 0 0 0.01 0.01
Rat 5 0 0.01 0.01 0.01 0
Rat 6 0 0.01 0.01 0.01 0.01
Rat 7 0 0 0.01 0 0
Rat 8 0 0 0.01 0 0
Mean 0 0 0 0 0
 Difference 0 0 0 0
% Difference 78.9 94.7 94.7 78.9
 foot volume

0.01

0
0
0
0
0
Column D

Column E

Column F

time
PARACETAMOL

Column G

Column H
Row 5
Row 3
Row 2
Row 1
 foot volume

0
0
0
0
0
0
0
0
0
Column D

Column E

Column F

time
ASPIRIN

Column G

Column H
Row 1
Row 9
Row 8
Row 6
 foot volume

0.01
0.01

0
0
0
0
0
Column D

Column E

Column F

time
CELECOXIB

Column G

Column H
Row 1
Row 1
Row 1
Row 1
 PREDNISONE
0.01
0
0
0
foot volume

0
0 Row 179
0 Row 192
0 Row 204
0
Row 216
0
0

Column G

Column H
Column E
Column D

Column F

time
 Row 14
Row 27
Row 39
Row 51
Column H
 Row 69
Row 82
Row 94
Row 106
Column H
 Row 124
Row 137
Row 149
Row 161
Column H
 Row 179
Row 192
Row 204
Row 216
Column H
 Dosage % Response
(mg) log (mg) After Carrageenan 1st hour 2nd hour
0.5 -0.3 80.0 173.3 126.7
1.6 0.2 100.0 192.9 142.9
A
5 0.7 188.9 255.6 255.6
16 1.2 110.0 280.0 320.0
0.5 -0.3 38.5 84.6 -38.5
1.6 0.2 29.2 -20.8 -20.8
B
5 0.7 7.4 -14.8 -22.2
16 1.2 -33.3 13.3 6.7
0.3 -0.52 115.4 130.8 76.9
1 0 55.2 3.4 -34.5
C
3 0.48 51.6 16.1 -12.9
10.0 1 4.9 -7.3 -39.0
0.02 -1.7 38.1 90.5 -4.8
0.06 -1.22 82.4 105.9 70.6
D
0.2 -0.7 93.8 93.8 112.5
0.6 -0.22 78.9 94.7 94.7

Paracetamol
325.0

300.0

275.0
% Response

250.0

225.0 Column D
200.0 Column E
Column F
175.0
Column G
150.0
C
125.0

100.0

75.0
0 2 4 6 8 10 12 14 16

Dosage (mg)

Aspirin
90.0
80.0
70.0
60.0
% Response

50.0
40.0
30.0 Column D
20.0 Column E
10.0 Column F
0.0 Column G
-10.0
 60.0

% Response
50.0
40.0
30.0 Column D
20.0 Column E
10.0 Column F
0.0 Column G
-10.0
-20.0
-30.0
-40.0
-50.0
0 2 4 6 8 10 12 14 16

Dosage (mg)

Celecoxib
140.0

120.0

100.0
% Response

80.0

60.0 Column D
40.0 Column E
Column F
20.0
Column G
0.0

-20.0

-40.0

-60.0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0

Dosage (mg)

Prednisone
120.0
110.0
100.0
90.0
% Response

80.0
70.0
Column D
60.0 Column E
50.0 Column F
40.0 Column G
30.0
20.0
10.0
0.0
-10.0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55 0.6
 50.0 Column F

% Re
40.0 Column G
30.0
20.0
10.0
0.0
-10.0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55 0.6

Dosage (mg)

Paracetamol
325.0
300.0
275.0
% Response

250.0
225.0
Column D
200.0 Column E
175.0 Column F
Column G
150.0
125.0
100.0
75.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage

Aspirin
90.0
80.0
70.0
60.0
% Response

50.0
40.0
30.0 Column D
20.0 Column E
10.0 Column F
0.0
Column G
-10.0
-20.0
-30.0
-40.0
-50.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage
 -40.0
-50.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage

Celecoxib
140.0

120.0

100.0
% Response

80.0

60.0 Column D
40.0 Column E
Column F
20.0
Column G
0.0

-20.0

-40.0

-60.0
-0.75 -0.5 -0.25 0 0.25 0.5 0.75 1

log of dosage

Prednisone
120.0
110.0
100.0
90.0
% Response

80.0
70.0 Column D
60.0 Column E
50.0 Column F
40.0 Column G
30.0
20.0
10.0
0.0
-10.0
-1.8 -1.6 -1.4 -1.2 -1 -0.8 -0.6 -0.4 -0.2

log of dosage
 3rd hour
180.0
192.9
322.2
290.0
23.1
-33.3
-48.1
33.3
84.6
3.4
-41.9
-31.7
23.8
76.5
62.5
78.9
B

After Carageenan injection

190.0
180.0
170.0
% Response

160.0
150.0
140.0
Column D
130.0
Column E
Column F 120.0
Column G 110.0
100.0
D 90.0
80.0
70.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
16 0.4 0.3 0.2 0.1

Dosage (mg)

After 1 hour
280.0
270.0
260.0
% Response

250.0
240.0
Column D 230.0
Column E
220.0
Column F
210.0
Column G
200.0
 250.0

% Respons
240.0
Column D 230.0
Column E
220.0
Column F
210.0
Column G
200.0
190.0
180.0
170.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
16 0.4 0.3 0.2 0.1

Dosage (mg)

After 2 hours
320.0
300.0
280.0
% Response

260.0
240.0
Column D
Column E 220.0
Column F 200.0
Column G 180.0
160.0
140.0
120.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0 10.0 0.4 0.3 0.2 0.1

Dosage (mg)

After 3 hours
330.0
320.0
310.0
300.0
% Response

290.0
280.0
270.0
Column D 260.0
Column E 250.0
240.0
Column F
230.0
Column G 220.0
210.0
200.0
190.0
180.0
170.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
.55 0.6 0.4 0.3 0.2 0.1
 240.0

% Re
Column F
230.0
Column G 220.0
210.0
200.0
190.0
180.0
170.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
.55 0.6 0.4 0.3 0.2 0.1

Dosage (mg)

Column D
Column E
Column F
Column G

1.2 1.3

Column D
Column E
Column F
Column G

1.2 1.3
1.2 1.3

Column D
Column E
Column F
Column G

Column D
Column E
Column F
Column G

-0.2
jection

Column D

0.9 1 1.1 1.2 1.3

Column E
 Column E

0.9 1 1.1 1.2 1.3

Column F

0.9 1 1.1 1.2 1.3

Column G

0.9 1 1.1 1.2 1.3

 Column G

0.9 1 1.1 1.2 1.3

 Dosage % Inhibition of Inflammation
(mg) log (mg) After Carrageenan 1st hour 2nd hour
0.5 -0.3 -80.0 -173.3 -126.7
1.6 0.2 -100.0 -192.9 -142.9
A
5 0.7 -188.9 -255.6 -255.6
16 1.2 -110.0 -280.0 -320.0
0.5 -0.3 -38.5 -84.6 38.5
1.6 0.2 -29.2 20.8 20.8
B
5 0.7 -7.4 14.8 22.2
16 1.2 33.3 -13.3 -6.7
0.3 -0.52 -115.4 -130.8 -76.9
1 0 -55.2 -3.4 34.5
C
3 0.48 -51.6 -16.1 12.9
10.0 1 -4.9 7.3 39.0
0.02 -1.7 -38.1 -90.5 4.8
0.06 -1.22 -82.4 -105.9 -70.6
D
0.2 -0.7 -93.8 -93.8 -112.5
0.6 -0.22 -78.9 -94.7 -94.7
% inhibition of inflammation

After Carageenan injection

-80.0
-90.0
-100.0
-110.0
-120.0
-130.0
-140.0 Column D
-150.0
-160.0
C -170.0
-180.0
-190.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage
ibition of inflammation

After 1 hour
-170.0
-180.0
-190.0
-200.0
-210.0
-220.0
-230.0 Column E
-240.0
-250.0
-260.0
 % inhibition of inf
-210.0
-220.0
-230.0 Column E
-240.0
-250.0
-260.0
-270.0
-280.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage
% inhibition of inflammation

After 2 hours
-120.0
-140.0
-160.0
-180.0
-200.0
-220.0
Column F
-240.0
-260.0
-280.0
-300.0
-320.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage
% inhibition of inflammation

After 3 hours
-170.0
-180.0
-190.0
-200.0
-210.0
-220.0
-230.0
-240.0
-250.0
-260.0 Column G
-270.0
-280.0
-290.0
-300.0
-310.0
-320.0
-330.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage
 -300.0

% inhibi
-310.0
-320.0
-330.0
- - - - 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
0.4 0.3 0.2 0.1

log of dosage
on
3rd hour
-180.0 80.0 173.3 126.7 180.0
-192.9 100.0 192.9 142.9 192.9
-322.2 188.9 255.6 255.6 322.2
-290.0 110.0 280.0 320.0 290.0
-23.1 38.5 84.6 -38.5 23.1
33.3 29.2 -20.8 -20.8 -33.3
48.1 7.4 -14.8 -22.2 -48.1
-33.3 -33.3 13.3 6.7 33.3
-84.6 115.4 130.8 76.9 84.6
-3.4 55.2 3.4 -34.5 3.4
41.9 51.6 16.1 -12.9 -41.9
31.7 4.9 -7.3 -39.0 -31.7
-23.8 38.1 90.5 -4.8 23.8
-76.5 82.4 105.9 70.6 76.5
-62.5 93.8 93.8 112.5 62.5
-78.9 78.9 94.7 94.7 78.9
B

Column D

1 1.2 1.3

Column E
 Column E

.1 1.2 1.3

Column F

.1 1.2 1.3

Column G

1 1.2 1.3
1 1.2 1.3