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    E05 Translation

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    api-27589736
    The document outlines the key requirements for translation, including an mRNA template, ribosome, 20 amino acids, initiation and elongation factors, start and stop codons, charged tRNAs, GTP, aminoacyl-tRNA synthetase, peptidyl transferase, and the four stages of translation: tRNA charging, initiation, elongation, and termination. It also compares differences in transcription and translation between prokaryotes and eukaryotes such as polycistronic vs monosistronic mRNA, presence of introns, location of transcription vs translation, and starting amino acids.

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    E05 Translation

    Uploaded by

    api-27589736
    20 views15 pages
    The document outlines the key requirements for translation, including an mRNA template, ribosome, 20 amino acids, initiation and elongation factors, start and stop codons, charged tRNAs, GTP, aminoacyl-tRNA synthetase, peptidyl transferase, and the four stages of translation: tRNA charging, initiation, elongation, and termination. It also compares differences in transcription and translation between prokaryotes and eukaryotes such as polycistronic vs monosistronic mRNA, presence of introns, location of transcription vs translation, and starting amino acids.

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    The document outlines the key requirements for translation, including an mRNA template, ribosome, 20 amino acids, initiation and elongation factors, start and stop codons, charged tRNAs, GTP, aminoacyl-tRNA synthetase, peptidyl transferase, and the four stages of translation: tRNA charging, initiation, elongation, and termination. It also compares differences in transcription and translation between prokaryotes and eukaryotes such as polycistronic vs monosistronic mRNA, presence of introns, location of transcription vs translation, and starting amino acids.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    20 views15 pages

    E05 Translation

    Uploaded by

    api-27589736
    The document outlines the key requirements for translation, including an mRNA template, ribosome, 20 amino acids, initiation and elongation factors, start and stop codons, charged tRNAs, GTP, aminoacyl-tRNA synthetase, peptidyl transferase, and the four stages of translation: tRNA charging, initiation, elongation, and termination. It also compares differences in transcription and translation between prokaryotes and eukaryotes such as polycistronic vs monosistronic mRNA, presence of introns, location of transcription vs translation, and starting amino acids.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
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    of 15

    TRANSLATION

    Requirements:

    • Template – mRNA for codon presentation


    • Ribosome (rRNA + protein)
    • 20 amino acids
    • Initiation and elongation factors
    • Start codon (AUG) and termination codons (UAG, UAA, UGA)
    • Charged tRNA and anticodon presentation
    • GTP
    • Aminoasil-tRNA synthetase and peptidyl transferase
    • 4 stages – tRNA charging, initiation, elongation and
    termination
    DIFFERENCES IN TRANSCRIPTION AND TRANSLATION IN
    PROKARYOTE AND EUKARYOTE

    Prokaryote Eukaryote

    Polycistronic mRNA Monosistronic mRNA

    Exon only Exon and intron

    Transcription and translation Transcription in nucleus and


    concurrent in cytoplasm translation in cytoplasm

    Starting AA formyl-methionine Starting AA methionine

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