GROUP 3

BioTech 111

WORKSHEET Laboratory Exercise 5 Genomic DNA Extraction 1. Check whether you have extracted DNA on your samples. Then rank the sample crop which has the most DNA extracted. Samples Yes DNA Extracted No Rank

Chayote Cabbage Banana Fruit Carrots Banana Leaves 2. Explain why DNA extraction is important in the field of biotechnology. It is the first step in the analysis and manipulation of DNA that allows scientist to detect genetic disorders, produce DNA fingerprints of individual and even create genetically engineered organism that can produce beneficial products such as insulin, antibiotic, and hormones. 3. Describe the appearance of the DNA you have extracted in this experiment? DNA appears as then threads. During our experiment we have seen DNA it looks like a flem. 4. Describe the function of the following materials in DNA extraction. Materials Function Salt Provides the DNA with a favorable environment; it contributes positively charge atoms that neutralize the normal negative charge of DNA. Cold Water Helps keep the DNA intact during the extraction process. Liquid Detergent Breaking down the lipid molecules of the cell and nuclear members, releasing the contents of the cell, including the DNA. Enzyme Are present in the cul cytoplasm (not the nucleus) to destroy the DNA of viruses that may enter our cell and make us sick. Alcohol DNA will not dissolve in this alcohol, so the DNA comes out of the solution, or precipitates. It is less dimes water or cell scum, which is what settles to the bottom of the glass, so it floats up into alcohol layer, where you see it as a snotty, string like substance, with small bubbles formed on it.