API ZYM

API ZYM

API ZYM

marker pen

. (
, , , biological fluid, etc)

19

.
20 well well

. spectrophotometric
, electrophoretic
.

- API ZYM 20
.

Suspensio
n Medium
2 ml

Demineralized water

NaCl
0.85%
Medium
2 ml

Sodium chloride
Demineralized water

8.5 g
1000 ml

ZYM
reagent
8 ml

A Tris-hydroxymethyl-aminomethane
25 g
Hydrochloric acid (37 %)
11 ml
Sodium lauryl sulfate
10 g
H2O
100 ml

ZYM
reagent
8 ml

B Fast Blue BB
2-methoxyethanol

0.35 g
100 ml

-
.

2~8 C

.
- .

2~8 C

( ZYM A 2~30 C .)

Kit (25)

API ZYM 25 strips

25

ZYM B

25

Package insert

. ZYM B
.
2~8 C ZYM A ,

()
-

Suspension Medium, 2ml (ref. 70700)

NaCl 0.85% Medium, 2ml (ref. 20070)

-

Reagent kit : ZYM A(ref. 70470)

ZYM B (ref. 70480)

-

Pipettes or PSIpettes (ref. 70250)

McFarland Standard (ref. 70 900)

. (20-30C)

DENSIMAT (ref. 70900)

-

Ampoule stand (ref. 70200)

,
.

35~37C incubator

- Refrigerator
- Bunsen burner

1/4

, .

bioMerieux Korea

API ZYM

- .

( )

Incubation box tray

.

- .
-

tray .

tray

37C, 4 .

.
.

ZYM A ZYM B
.

NCCLS M29-A, Protection of Laboratory Workers from

, , ,

ZYM A ZYM B
.

Instrument Biohazards and Infectious Disease Transmitted

5 .

by Blood, Body Fluids, and Tissue; Approved Guideline-

, 10 cm (1000 W

December 1997 Biosafety

bulb) 10 .

in Microbiological and Biomedical laboratories, HHS

fast Blue BB

Publication No.(CDC) 93-8395, 3rd Edition (May 1993)

, .

.
0~5 .

0 , 5 1, 2, 3, 4
3 .

saline 2ml .

24

For microorganisms :

Suspension medium(2ml),

API ZYM
.

5-6 McFarland .
Slant agar

bioMerieux API ZYM

.

For other specimens (Cell suspension,

QC

tissues, biological fluids,..)

2/4

bioMerieux Korea

API ZYM

.

. API ZYM
, .
1.

Pseudomonas aeruginosa ATCC 27853

(Profile obtained after 18-24 hr. culture on trypcase soy
agar. Inoculum adjusted to between 5 and 6 McF using
DENSIMAT)

2.

Beta-glucosidase Sigma G0395

(Profile obtained using a concentraion of 0.2 g/l)

3.

Alpha- chymotrypsin Sigma C4129

(Profile obtained using a concentration of 1 g/l)

No.

Enzyme Assayed For

Substrate

3/4

pH

Result

bioMerieux Korea

API ZYM

positive negative
1

Control

Colorless or color
of the sample
if it has an
intense coloration

Alkaline phosphatase

2-naphthyl phosphate

8.5

Esterase (C4)

2-naphthyl butyrate

6.5 Violet

Esterase Lipase (C8)

2-naphthyl caprylate

7.5

Lipase (C14)

Leucine arylamidase

2-naphthyl myristate
L-leucyl-2-naphthylamide

Violet
Orange

Valine arylamidase

L-valyl-2-naphthylamide

Orange

Crystine arylamidase

L-cystyl-2-naphthylamide

Orange

Trypsin

N-benzoyl-DL-arginine-2naphthylamide

8.5

Orange

Colorless

10

chymotrypsin

N-glutaryl-phenylanine-2naphthylamide

7.5

Orange

Or

11

Acid phospatase

2-naphtyl phosphate

5.4

Violet

12

Naphtol-AS-BIphosphohydrolase

Naphthol-AS-BI-phosphate

13

galactosidase

6-Br-2-naphthyl- Dgalactopyranoside

Violet

14

- galactosidase

2-naphthyl- Dgalactopyranoside

Violet

15

- glucuronidase

Naphthol-AS-BI- Dglucuronide

Blue

16

glucosidase

2-naphthyl- Dglucopyranoside

Violet

17

- glucosidase

6-Br-2-naphthyl- Dglucopyranoside

Violet

18

N acetyl -

1-naphthyl-N-acetyl- Dglucosaminide

Brown

6-Br-2-naphthyl- Dmannopyranoside
2-naphthyl- Lfucopyranoside

Violet

Violet

- glucosaminidase

19

mannosidase

20

fucosidase

Violet
Violet

Very pale
Yellow*

control
.

4/4

bioMerieux Korea