Code: R7312302

R7
BIOCHEMICAL REACTION ENGINEERING-I (Bio-Technology)

III B.Tech I Semester (R07) Supplementary Examinations, November 2011

Time: 3 hours Answer any FIVE questions All questions carry equal marks *****
1 (a) (b)

Max Marks: 80

(c)

Distinguish between elementary and non elementary reactions with examples. For a gas reaction at 400k the rate is reported as = 3.66 PA2, atm/hr. (i)What are the units of the rate constant? (ii)What is the value of the rate constant for this reaction if the rate equation is expressed as -rA = =R CA2 mol/m3. S At 1100 K n-nonane thermally cracks 20 times as rapidly as at 1000 k. Find the activation energy for this decomposition. Give a brief account of batch, fed-batch and continuous mode of operation of reaction system for studying growth kinetics. Discuss about substrate inhibition and effect of inhibitors on cell growth. Give an account of models for substrate and product inhibition. Anaerobe degradation of an organic compound by a mixed culture of organisms in the waste water can be represented by the following reaction. C3 H6 O3 +a O2 +b NH3 c C5 H7 N O2 +d H2 0 +e C O2 Determine a,b,c,d and e if YX/S =0.4 gX/g s. Determine the yield coefficients Yx/o2 and Yx/NH3 Determine the degree of reduction for the substrate, bacteria, and RQ for the organisms. Consider the aqueous reactions A+B R desired dcR /dt =1.0CA1.5 CB0.3 mol/liter.min A+B S undesired dcS /dt =1.0CA0.5 CB1.8 mol/liter.min 0 For 90 conversion of A find the concentration of R in the product stream. Equal Volumetric flow rates of the A and B streams are fed to the reactor, and each stream has a concentration of 20 mol/liter of reactant. The flow in the reactor following (i) Plug flow (ii) Mixed flow Discuss in detail about the various types of inhibition and their effect on the kinetic parameter of Michaelis Menten equation with suitable figures.

3 (a) (b) 4

(a) (b) (b)

7 8 (a) (b)

Write about the physical and chemical techniques for enzyme immobilization. Write short notes on Filter and pore diffusion effects on kinetics of immobilized enzymes. Effectiveness factor and Thiele modules. *****