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    An operon is a group of genes that are under the control of a single promoter and are co-transcribed. In bacteria, gene expression is controlled by operons such as the lac and trp operons in response to environmental conditions. In eukaryotes, a single regulatory protein can control gene expression by binding to enhancer or silencer regions and recruiting other proteins to activate or repress transcription. Retrotransposons are DNA sequences that can move within genomes and replicate via an RNA intermediate. Common cloning vectors used in genetic engineering include plasmids, bacteriophages, and artificial chromosomes. A gene library contains DNA fragments with genomic DNA inserted into cloning vectors, while a cDNA library contains fragments of complementary DNA

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    An operon is a group of genes that are under the control of a single promoter and are co-transcribed. In bacteria, gene expression is controlled by operons such as the lac and trp operons in response to environmental conditions. In eukaryotes, a single regulatory protein can control gene expression by binding to enhancer or silencer regions and recruiting other proteins to activate or repress transcription. Retrotransposons are DNA sequences that can move within genomes and replicate via an RNA intermediate. Common cloning vectors used in genetic engineering include plasmids, bacteriophages, and artificial chromosomes. A gene library contains DNA fragments with genomic DNA inserted into cloning vectors, while a cDNA library contains fragments of complementary DNA

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    R7312304 Genetic Engineering

    Uploaded by

    sivabharathamurthy
    An operon is a group of genes that are under the control of a single promoter and are co-transcribed. In bacteria, gene expression is controlled by operons such as the lac and trp operons in response to environmental conditions. In eukaryotes, a single regulatory protein can control gene expression by binding to enhancer or silencer regions and recruiting other proteins to activate or repress transcription. Retrotransposons are DNA sequences that can move within genomes and replicate via an RNA intermediate. Common cloning vectors used in genetic engineering include plasmids, bacteriophages, and artificial chromosomes. A gene library contains DNA fragments with genomic DNA inserted into cloning vectors, while a cDNA library contains fragments of complementary DNA

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    Code: R7312304

    R7
    GENETIC ENGINEERING (Bio-Technology)

    III B.Tech I Semester (R07) Supplementary Examinations, November 2011

    Time: 3 hours Answer any FIVE questions All questions carry equal marks *****
    1

    Max Marks: 80

    How do you define an operan? Explain the Control of gene expression in bacteria by citing the example of any operon. Can you explain taking the example of a single type of regulatory protein, how the process of regulation of gene expression occurs in eukaryotes. What are retrotransposons? Explain in detail with examples. What are the different types of cloning vectors used in genetic engineering? Give a brief definition of a gene library. What is the essential difference between a genomic library and a cDNA library? List the major advantages/Limitations on the use of each. Write about primer designing in PCR. Which DNA polymerase is used in PCR? Using suitable example, explain the importance of 16S rRNA as a molecular marker.

    3 4 5

    6 7

    8 (a) Write the applications of genetic engineering in medicine and agriculture. (b) How do you develop transgenic animal? Mention its applications *****

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