The Liposome Expert CERTIFICATE OF ANALYSIS Caution: The Product Is For Research Only, Not For Human Use!

DESCRIPTION:

Cationic Liposomes DOTAP/DOPE

F50102 03311101 2 mL (2x1mL) 04/04/2011 DOTAP/DOPE (50:50 mol/mol)

Catalogue #:
BATCH #: PRODUCT VOLUME: DATE OF PRODUCTION: LIPID COMPOSITION:

ANALYTICAL DATA:
Total lipid conc.: DOTAP: DOPE: Particle size Zeta Potential pH Bulk solution: FORM/COLOR: STABILITY: 5.0 0.2mM (3.61 0.14 mg/mL) (Steward assay) 2.4 0.1 mM (1.75 0.07 mg/mL) (calculated from total lipid) 2.6 0.1 mM (1.86 0.07 mg/mL) (calculated from total lipid) Mean diameter: 71.4 1.0 nm; Half-width: 37 1.0 nm; Polydispersity: 0.27 0.01 (Brookhaven ZetaPALS) 25.2 2.0 mV (in 1mM NaCl, Brookhaven ZetaPALS) 5-5.5 (non-buffered) Milli-Q water Translucent, free flow liposomes. No visible particles/aggregates with naked eye or under microscope Product is sterile filtered (0.2m). Free from bacteria growth for 3 month for unopened vials when store at 2-8 C. Warm to room temperature and mix well before use.

METHOD OF PRODUCTION: Lipid thin-film hydrated in Milli-Q water. Down-sized by sonication.

DOTAP: 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt) (Avanti Polar Lipids, Cat# 890890) DOPE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (Avanti Polar Lipids, Cat#850725)

1|Page

FormuMax Scientific Inc. 4062 Fabian Way, Palo Alto, CA 94303 Tel: (650) 856-7948 Fax: (408) 465-5338 E-mail: service@FormuMax.com Homepage: www.FormuMax.com