Scribd Logo
Upload

Welcome to Scribd!

  • Langmuir: Ongeremd Geremd 1A 2A 1B 2B 3A 4A 3B 4B 5A 6A 5B 6B 7A 8A 7B 8B

    Uploaded by

    Charlotte Hornstra
    12 views3 pages
    This document contains data from experiments measuring substrate concentration and reaction rate for both uninhibited and inhibited enzyme reactions. Graphs are presented of reaction rate vs substrate concentration, the Lineweaver-Burk plot, and the Michaelis-Menten plot. Kinetic parameters Vmax and Km are calculated from the linear regions of the graphs. The data and graphs indicate that inhibition decreases both the Vmax and slope of the reaction.

    Original Description:

    Original Title

    001

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document contains data from experiments measuring substrate concentration and reaction rate for both uninhibited and inhibited enzyme reactions. Graphs are presented of reaction rate vs substrate concentration, the Lineweaver-Burk plot, and the Michaelis-Menten plot. Kinetic parameters Vmax and Km are calculated from the linear regions of the graphs. The data and graphs indicate that inhibition decreases both the Vmax and slope of the reaction.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    12 views3 pages

    Langmuir: Ongeremd Geremd 1A 2A 1B 2B 3A 4A 3B 4B 5A 6A 5B 6B 7A 8A 7B 8B

    Uploaded by

    Charlotte Hornstra
    This document contains data from experiments measuring substrate concentration and reaction rate for both uninhibited and inhibited enzyme reactions. Graphs are presented of reaction rate vs substrate concentration, the Lineweaver-Burk plot, and the Michaelis-Menten plot. Kinetic parameters Vmax and Km are calculated from the linear regions of the graphs. The data and graphs indicate that inhibition decreases both the Vmax and slope of the reaction.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 3

    Langmuir

    Langmuir

    Ongeremd

    Geremd
    [S]
    0,33
    0,33
    0,67
    0,67
    1,33
    1,33
    5,33
    5,33

    1A
    1B
    3A
    3B
    5A
    5B
    7A
    7B

    [S]/V
    0,14
    0,14
    0,22
    0,24
    0,37
    0,36
    0,78
    0,73

    [S]
    0,33
    0,33
    0,67
    0,67
    1,33
    1,33
    5,33
    5,33

    2A
    2B
    4A
    4B
    6A
    6B
    8A
    8B

    [S]/V
    0,61
    0,62
    0,75
    0,77
    1,05
    0,93
    1,58
    1,32

    Langmuir
    1,8
    1,6

    y = 0,1526x + 0,6615
    R = 0,8897

    1,4
    [S]/V

    1,2
    Geremd

    1
    0,8

    Ongeremd

    0,6

    Linear (Geremd)

    0,4

    y = 0,1156x + 0,1512
    R = 0,9686

    0,2
    0
    0

    3
    [S] (mol)

    Vmax=
    Km=

    Linear (Ongeremd)

    Lineweaver-Burk
    Ongeremd
    1/[S]
    1A
    3,03
    1B
    3,03
    3A
    1,49
    3B
    1,49
    5A
    0,75
    5B
    0,75
    7A
    0,18
    7B
    0,18

    Lineweaver-Burk
    Geremd
    1/[S]
    2A
    3,03
    2B
    3,03
    4A
    1,49
    4B
    1,49
    6A
    0,75
    6B
    0,75
    8A
    0,18
    8B
    0,18

    1/V
    0,44
    0,45
    0,33
    0,36
    0,28
    0,27
    0,14
    0,13

    1/V
    1,96
    1,88
    1,12
    1,16
    0,79
    0,70
    0,29
    0,24

    Lineweaver-Burk
    2,5
    y = 0,5639x + 0,2492
    R = 0,9856

    1/V

    1,5

    Omgeremd

    y = 0,1002x + 0,1635
    R = 0,8962

    0,5

    Linear (Omgeremd)
    Linear (Geremd)

    0
    0,00

    1,00

    2,00
    1/[S]

    Vmax=
    Km=

    Geremd

    3,00

    4,00

    Michaelis Menten

    Michaelis Menten

    Ongeremd

    Geremd
    [S]
    0,33
    0,33
    0,67
    0,67
    1,33
    1,33
    5,33
    5,33

    1A
    1B
    3A
    3B
    5A
    5B
    7A
    7B

    V
    2,23
    2,21
    3,02
    2,72
    3,51
    3,64
    6,77
    7,30

    [S]
    0,33
    0,33
    0,67
    0,67
    1,33
    1,33
    5,33
    5,33

    2A
    2B
    4A
    4B
    6A
    6B
    8A
    8B

    V
    0,51
    0,53
    0,89
    0,86
    1,26
    1,42
    3,36
    4,01

    Michaelis-Menten
    y = 1,7574ln(x) + 3,7276
    R = 0,937

    8
    7
    V(nmol/min)

    6
    5

    y = 1,1611ln(x) + 1,4746
    R = 0,9148

    Ongeremd
    Geremd

    Log. (Ongeremd)

    Log. (Geremd)

    1
    0
    0

    3
    [S] (mol)

    Vmax=
    Km=

    You might also like