T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate (ab14899)

URL for this product: http://www.abcam.com/T47D-Human-ductal-breast-epithelial-tumor-cell-line-Whole-Cell-Lysate-ab14899.html

Product overview

Description General notes

T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80C.

Background

T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.

Properties

Form Storage instructions Storage buffer

Liquid Shipped at 4C. Upon delivery aliquot and store at -20C or -80C. Avoid repeated freeze / thaw cycles. pH: 7.90 Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20% Glycerol, 0.009% PMSF, 0.008% DTT See the website for more information on MSDS for this product. Whole Cell Lysate

Purity

Applications

General application notes

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Our Abpromise to you: Quality guaranteed and expert technical support

If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.

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Last updated on February 16, 2012