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  • Differentiation Cutrate+ Citrate

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    Hagar_Microb
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    The document provides instructions for preparing a differentiation medium called Citrate+/Citrate- that can be used to identify bacterial species based on their ability to metabolize citrate. The medium contains a base prepared from tryptone, yeast extract, gelatin, glucose, lactose, NaCl, trisodium citrate, and calcium lactate in water at pH 6.6/6.7. It also includes additions of sterilized serum and a suspension of calcium citrate. Bacteria that can metabolize citrate will form a transparent halo around colonies on the plated medium after 3 days of incubation, while those that cannot metabolize citrate will form no halo.

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    The document provides instructions for preparing a differentiation medium called Citrate+/Citrate- that can be used to identify bacterial species based on their ability to metabolize citrate. The medium contains a base prepared from tryptone, yeast extract, gelatin, glucose, lactose, NaCl, trisodium citrate, and calcium lactate in water at pH 6.6/6.7. It also includes additions of sterilized serum and a suspension of calcium citrate. Bacteria that can metabolize citrate will form a transparent halo around colonies on the plated medium after 3 days of incubation, while those that cannot metabolize citrate will form no halo.

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    6 views2 pages

    Differentiation Cutrate+ Citrate

    Uploaded by

    Hagar_Microb
    The document provides instructions for preparing a differentiation medium called Citrate+/Citrate- that can be used to identify bacterial species based on their ability to metabolize citrate. The medium contains a base prepared from tryptone, yeast extract, gelatin, glucose, lactose, NaCl, trisodium citrate, and calcium lactate in water at pH 6.6/6.7. It also includes additions of sterilized serum and a suspension of calcium citrate. Bacteria that can metabolize citrate will form a transparent halo around colonies on the plated medium after 3 days of incubation, while those that cannot metabolize citrate will form no halo.

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    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    Differentiation Citrate+/Citrate1. Preparation of the medium Base A: Tryptone 20g Yeast extract 5g Gelatin 2.

    5g Glucose 5g Lactose 5g NaCl 4g Trisodium Citrate 2g Calcium Lactate 8g Agar 15g Distilled Water 1000ml Verify pH: 6.6/6.7 Sterilize for 15 minutes at 120C. Let cool to 50C. Base B: Serum: Obtain by paper filtration of a fresh starter and then sterilize for 15 minutes at 120C. Base C: Suspension of Calcium Citrate : Take Calcium Citrate 4H2O. Suspend 10g of Citrate in 100ml of 1.5% solution of Carboxymethylcellulose at 45-50C. Mix everything together. Sterilize for 15 minutes at 120C. For 100ml of Base A, add 5ml of Serum and 5ml of Calcium Citrate. The medium is used in population counting. Incubate for 3 days at 25C.

    Legend: Citrate+ : Lactococcus lactis subsp. lactis biovar diacetylactis Leuconostoc mesenteroides Presence of a transparent halo around the colony.

    Citrate+ : Lactococcus lactis subsp. lactis Leuconostoc mesenteroides subsp. Cremoris No halo.

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