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    Lecture21 111026

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    conmen2005
    This document contains the notes for Lecture 21 of Chemistry 4101, Fall 2011. The lecture covers the following topics: - Definitions of terminology used in chromatography such as mobile phase, stationary phase, bands or zones. - Theories of band broadening and equations for selectivity factor, retention factor, plate height, theoretical plates, and resolution. - Factors that affect column efficiency such as velocity of the mobile phase and eddy diffusion. - The general elution problem and solutions to chromatography equations.

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    Lecture21 111026

    Uploaded by

    conmen2005
    24 views2 pages
    This document contains the notes for Lecture 21 of Chemistry 4101, Fall 2011. The lecture covers the following topics: - Definitions of terminology used in chromatography such as mobile phase, stationary phase, bands or zones. - Theories of band broadening and equations for selectivity factor, retention factor, plate height, theoretical plates, and resolution. - Factors that affect column efficiency such as velocity of the mobile phase and eddy diffusion. - The general elution problem and solutions to chromatography equations.

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    This document contains the notes for Lecture 21 of Chemistry 4101, Fall 2011. The lecture covers the following topics: - Definitions of terminology used in chromatography such as mobile phase, stationary phase, bands or zones. - Theories of band broadening and equations for selectivity factor, retention factor, plate height, theoretical plates, and resolution. - Factors that affect column efficiency such as velocity of the mobile phase and eddy diffusion. - The general elution problem and solutions to chromatography equations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    24 views2 pages

    Lecture21 111026

    Uploaded by

    conmen2005
    This document contains the notes for Lecture 21 of Chemistry 4101, Fall 2011. The lecture covers the following topics: - Definitions of terminology used in chromatography such as mobile phase, stationary phase, bands or zones. - Theories of band broadening and equations for selectivity factor, retention factor, plate height, theoretical plates, and resolution. - Factors that affect column efficiency such as velocity of the mobile phase and eddy diffusion. - The general elution problem and solutions to chromatography equations.

    Copyright:

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    of 2

    10/24/11

    10/24/11

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Chem 4101 Fall 2011

    Selectivity Factor

    Lecture 21 Oct 26

    Chromatography
    Equations 26-13, 26-14, and 26-15 !

    1- Definitions 2- Theory of band broadening 3- General elution problem


    A!

    "=

    K B k B t R,B # t M = = K A k A t R, A # t M

    B!

    A!

    B!

    -All sections in Chapter 26 Suggested exercises: 26-1, 26-6, 26-9, 26-11, 26-12, 26-14, 26-15, 26-17, 26-20

    Figure 26-2, Sections 26A, 26B

    Chem 4101 Fall 2011

    Terminology
    Mobile phase eluent! Stationary phase! Bands or zones!

    Lecture 21 Oct 26

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Plate height and theoretical Plates

    Equations 26-16, 26-21, and 26-22 ! Analyte dilution! Dead or void time (tM)! Elution time (tR)! Chromatogram! Distribution constant! Retention factor! Selectivity! Figure 26-1, Section 26A Figure 26-7, Sections 26C

    N=

    # t &2 # t &2 L = 16 " % R ( = 5.54 " % R ( % ( H $ W1/ 2 ' $W '

    Chem 4101 Fall 2011

    Retention factors

    Lecture 21 Oct 26

    Chem 4101 Fall 2011

    Resolution

    Lecture 21 Oct 26

    Equations 26-24 to 26-26 !

    RS =

    "Z WA WB + 2 2

    Equation 26-3, tR = tM + tS !

    RS = 2

    [t

    R,B

    " t R, A ]

    WA + WB

    !
    Equations 26-4 and 26-5 ! Equations 26-9 and 26-10, 26-12 !

    RS =

    N $ " #1' $ k B ' & )& ) 4 % " ( % 1+ k B (

    "=

    L tR

    u=

    L tM

    kA =

    K A VS VM

    "=u#

    1 1+ k A

    kA =

    tR " tM tM

    !
    Also see Equations 26-26 to 26-28 ! Figure 26-12, Sections 26D

    Figure 26-4, Section 26B

    10/24/11

    10/24/11

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Chem 4101 Fall 2011

    Summary Table 26-5

    Theory of column efficiency

    Lecture 21 Oct 26

    Master Example 26-1 and related exercises

    Sectios 26E

    Figure 26-10, Sections 26C-3

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Theory of column efficiency


    B H = A + + CSu + CMu u

    Variables that affect column efficiency

    Table 26-3, Section 26C

    Section 26C

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Chem 4101 Fall 2011

    Lecture 21 Oct 26

    Fundamentals of column efficiency Eddy diffusion


    -moderate to high velocities of mobile phase - stagnant pools of mobile phase

    The General Elution Problem- solutions

    Figure 26-9, Section 26C

    Figure 26-14, Sections 26D

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