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Effect of PH Factor On The Growth of Microorganisms: Chapter 1 (Microorganism) 1
Effect of PH Factor On The Growth of Microorganisms: Chapter 1 (Microorganism) 1
Effect of PH Factor On The Growth of Microorganisms: Chapter 1 (Microorganism) 1
Class: ________
CHAPTER 1 (MICROORGANISM)
1. Effect of pH factor on the growth of microorganisms
Aim : ____________________________________________________________________________________________.
Problem statement : What is the effect of pH value on the growth of bacteria?
Hypothesis : ______________________________________________________________________________________
Variable:
Manipulated : _____________________________________________.
Responding
: _____________________________________________.
Constant
: _____________________________________________.
1.
_____________________
5.
_______________________
2.
_____________________
6.
_______________________
3.
_____________________
7.
______________________
4.
_____________________
Sterile
cotton
sterile
cotton
nutrient broth +
Bacteria culture
A
acidic
nutrient broth +
Bacteria culture
B
pH 7 (neutral)
nutrient broth +
Bacteria culture
C
Alkaline
Procedure:
1. Three test tubes with nutrient broth are labelled A, B and C.
2.
3.
4.
5.
Tabulation of data:
Conclusion:
___________________________________________________________________________________
Name: _________________________________________
Class: ________
_____________________________________________
Responds
_____________________________________________
Constant
_____________________________________________
1.
_____________________
5.
_______________________
2.
_____________________
6.
_______________________
3.
_____________________
7.
______________________
4.
_____________________
Sterile cotton
nutrient broth +
Bacteria culture
A (50C )
nutrient broth +
Bacteria culture
B (370C )
nutrient broth +
Bacteria culture
C (600C )
Procedure:
1. Three test tubes with nutrient broth are labelled A, B and C.
2. 1 ml of bacterial culture is poured onto the nutrient broth in each test tube.
3. The test tubes are covered with a steril cotton.
4. The test tube are placed in various places as stated below:
A: kept in a refrigerator at temperature 50C
B: kept in an oven at temperature 370C
C: kept in an oven at temperature 800C
5. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:
Conclusion:
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
_____________________________________________________________________________.
3. Effect of light on the growth of microorganisms:
Aim : __________________________________________________________________________.
Problem statement : What is the effect of light on the growth of bacteria?
Hypothesis : ____________________________________________________________________.
Variable:
Manipulated:
____________________________
Responds :
____________________________
Constant
____________________________
____________________
2.
____________________
3.
____________________
4. ____________________
5.
___________________
sterile cotton
nutrient broth +
Bacteria culture
A (dark)
nutrient broth +
Bacteria culture
B (under bright light)
Procedure:
1. Two test tubes with nutrient broth are labelled A and B.
2. 1 ml of bacterial culture is poured onto the nutrient broth in each test tube.
3. The test tubes are covered with a steril cotton.
4. The test tubes are placed in various places as stated below:
A: is placed in a dark condition
B: is left under bright light.
5. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:
Conclusion:
____________________________________________________________________________.
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
____________________________________
Responds :
____________________________________
Constant
____________________________________
_____________________
4. ________________
2.
_____________________
5. _________________
3.
____________________
6. _________________
sterile cotton
Damp nutrient broth +
Bacteria culture
A (humid)
Procedure:
1. Two test tubes are labelled A and B, and their contents are shown below.
A: Damp nutrient broth + 1 ml of bacterial culture
B: Dry nutrient broth + 1 ml of bacterial culture
2. The test tubes are covered with a steril cotton.
3. All the three test tubes are kept in a cupboard.
4. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:
Conclusion:
_______________________________________________________________________________.
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
______________________
Responds :
______________________
Constant
_______________________
____________________
2.
____________________
3.
____________________
5. ___________________
4.
___________________
sterile cotton
Nutrient broth +
Bacteria culture
A
(presence of nutrient)
B
(without nutrient)
Procedure:
1. Two test tubes are labelled A and B, and their contents are shown below.
A: damp nutrient broth + 1 ml of bacterial culture
B: damp broth without nutrient + 1 ml of bacterial culture
2. The test tubes are covered with a steril cotton.
3. All the three test tubes are kept in a cupboard.
4. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:
Conclusion:
____________________________________________________________________________.
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
_________________________________
Responds :
_________________________________
Constant
__________________________________
distil water
disc
_______________________
5. ______________________
2.
______________________
6. ______________________
3.
______________________
7.
4.
______________________
_____________________
nutrient agar
+
bacteria culture
penicillin disc
Procedure:
1.
2.
1 ml of bacterial culture is poured onto the nutrient agar in each Petri dish.
3. A dish of filter paper that has been moistened with distil water is placed on the surface of
nutrient agar in Petri dish A as shown above.
4. A penicillin disc is placed on the surface of nutrient agar in Petri dish B.
5.
6.
Tabulation of data:
Conclusion:
______________________________________________________________________________.
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
CAPTER 5 (MOTION)
A) INERTIA
Aim: ___________________________________________________________________________________.
Problem statement: What is the relationship between mass and inertia?
Hypothesis: _____________________________________________________________________________.
Variable:
Manipulated : _____________________________________________.
Responding
: _____________________________________________.
Constant
: _____________________________________________.
Procedure:
1. Tin B is filled up with sand while tin A is left empty.
2. Both tins are swung at the same height.
3. The tin are stopped from swinging after a while.
4. The tin that is harder to swing and that is harder to stop are observed.
Tabulation Of Data:
Conclusion:
________________________________________________________________________________.
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
MOMENTUM
Relationship Between Mass And Momentum
Aim: ___________________________________________________________________________.
Problem statement: What is the relationship between mass and momentum?
Hypothesis: ______________________________________________________________________.
Variable:
Manipulated variable : ____________________________.
Responding variable : ____________________________
Constant variable
: ____________________________
(a)
(b)
Procedure:
1. The apparatus as shown in diagram (a) is set up.
2. A trolley is pushed gently to move down the track and collides a wooden block.
3. The displacement of wooden block is measured and recorded.
4. This experiment is repeated by using a two-stacked trolley as shown in diagram (b).
Tabulation Of Data:
Conclusion:
_____________________________________________________
Prepared by Pn. Sity Salang
Name: _________________________________________
Class: ________
: _____________________________________________________
(a)
(b)
Procedure:
1. The apparatus as shown in diagram (a) is set up.
2. A trolley is pushed gently down the track and it collides with a wooden block.
3. The displacement of wooden block is measured and recorded
4. This experiment is repeated by using two wooden blocks to make the track steeper as shown in
diagram (b).
Tabulation Of Data:
Conclusion:
Name: _________________________________________
Class: ________
_______________________________________________________________________________.
C) PRESSURE
1. Relationship Between Pressure And Force
Aim: ___________________________________________________________________________.
Problem statement: What is the relationship between pressure and force?
Hypothesis: _____________________________________________________________________.
Variable:
Manipulated : ____________________________________________________
Responding : ____________________________________________________
Constant
: _____________________________________________________
Procedure:
1.
2.
3.
4.
Steps 2 and 3 are repeated with various loads of 200g, 300g, 400g and 500g.
5.
Tabulation Of Data:
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Name: _________________________________________
Class: ________
Conlusion:
___________________________________________________________________
2. Relationship Between Pressure And surface Area.
Aim: __________________________________________________________________________
Problem statement: What is the relationship between pressure and surface area?
Hypothesis: ____________________________________________________________________.
Variable:
Manipulated : ____________________________________________________
Responding : ____________________________________________________
Constant
: _____________________________________________________
2.
_______________________
2. ____________________
4.
_______________________
Procedure:
1.
A 500g weight on top of the plasticine as shown above. The impact on the plasticine is
recorded.
2.
The experiment is repeated by putting the weight on a sharp nail and a blunt nail respectively,
while their pointed edges press against the plasticine.
3.
4.
Tabulation Of Data:
11
Name: _________________________________________
Class: ________
Conlusion:
__________________________________________________________________________.
Aim: To study Archimedes principle.
Problem statement: What is the relationship between the buoyant force and the weight of the water
displaced?
Hypothesis: The buoyant force acting on the stone is equal to the weight of the water displaced by the
stone.
Variable:
Manipulated : Weight of stone
Responding : Weight of water displaced.
Constant
2.
Beaker
3. Thread
4.
5. Spring balance
6. Water
Procedure:
1.
2.
3.
4.
Immerse the stone into the water in the Eureka Vessel as shown in diagram (b).
5.
6.
Measure the weight of the beaker that contains the displaced water and record it as W2.
7.
8.
12
Name: _________________________________________
9.
Record the observations in the form of the table.
Class: ________
Result:
Weight of stone in air = 0.8 N (W3)
Weight of stone in water = 0.4 N (W4)
(appearance weight loss of the stone)
Buoyant force acting on the stone = 0.4 N (W3 - W4)
Weight of empty beaker = 0.6 N (W1)
Weight of beaker and water displaced = 1.0 N (W5)
Weight of water displaced = 0.4 N (W5 - W1)
From the results, W4 < W3
W3 - W4 = W5 - W1
Weight Of Stone in the water (N)
0.4
0.2
Discussion:
From the experiment, it is observed that volume of water displaced is equal to the volume of
the object that is completely immersed in water.
The weight of the stone when it is completely immersed in water is less than the natural
weight of the stone in air. This is due to the upthrust or buoyant force of the water acting on the stone.
The amount of water in the beaker is the amount of water displaced by the stone. The weight
loss of the stone is equal to the weight of the water displaced.
Conlusion:
The buoyant force acting on the stone is equal to the weight of the water displaced by the
stone. Hypothesis is accepted.
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