H v tn sinh vin:

T Kim Cn 1014021
M s dung dch kim tra: 07
L Ngc Cng 1014029
M s dung dch kim tra: 65
M s nhm thc tp: CN N 2
Ch nht, ngy 11 thng 3 nm 2012

BI TNG TRNH PHN TCH NH LNG

PHNG PHP CHUN COMPLEXON
1. Vi gii thiu ban u:
1.1. Dung dch m:
Phn ng chun lun c thc hin ti mt gi tr pH rt xc nh ca dung dch m
thch hp.
V d chun Mg2+ bng EDTA ti pH = 10:
Mg2+ + H2Y2- MgY + 2H+
Phn ng cho thy vi mi mol Mg2+ tham gia phn ng li gii phng 2 mol H+ i vo
trong dung dch. Vy phi c dung dch m duy tr pH n nh, trnh s thay i ca cc
hng s iu kin lm sai lch kt qu chun .
1.2. Cc lu khc:
Chun complexom l phng php phn tch nh lng dng xc nh nng ca
cc hu ht cc ion kim loi ni chung, tr kim loi kim. EDTA phn ng to phc bn vi hu
ht cc kim loi theo t l 1:1 nn rt thun li trong vic tnh ton kt qu chun .
Phng php chun complexom c nhiu u im trong vic xc nh hm lng cc
ion kim loi trong dung dch. Tuy nhin phng php ny hn ch trong vic xc nh hn hp
cc kim loi khng tinh khit (v EDTA phn ng hu ht vi cc kim loi nn tnh chon lc
khng cao). Do ngi ta c th nng cao tnh chon lc bng vic s dng cht che.
C nhiu phng php chun complexom nh: chun trc tip, chun thay
th, chun ngc... mi phng php c p dng trong tng trng hp ring bit ty
thuc vo i tng cn xc nh, iu kin hin c ca phng th nghim...
2. Pha cc dung dch v ch th mu kim loi:
2.1. Gii thiu:
Dung dch ETDA ban u do phng th nghim chun b c nng c 1.000000N, ta
pha long thnh cc dung dch EDTA c nng thp hn s dng cho cc th nghim t y
tr v sau.
2.2. Pha dung dch ETDA nng 0.01N:
Ly ng 5ml dung dch ETDA ban u (do phng th nghim cung cp) nh mc trong
bnh 500ml:

CEDTA = ( 1.000000 0.000005 ) M

N EDTA = ( 1.000000 0.000005 ) N

buret
0.030
= 1.96*
= 0.059
1
1
= ( 5.000 0.059 ) ml

0.95,V buret = z0.95 *

Vburet = VEDTA

bnh 500ml
0.050
= 1.96*
= 0.098
1
1
= ( 500.000 0.098 ) ml

0.95,V bnh 500ml = z0.95 *

Vbnh 500ml

N EDTA 0.01N =

VEDTA 1N *N EDTA 1N 5.000*1.000000

=
= 0.01
Vbnh 500ml
500.000
2

0.95, N EDTA 0.01N

N
N
N

= EDTA 0.01N * VEDTA 1N + EDTA 0.01N * N EDTA 1N + EDTA 0.01N * V bnh 500ml
VEDTA 1N
NEDTA 1N
Vbnh 500ml

0.01
0.01
0.01

=
*0.059 +
*0.000005 +
*0.098 = 0.00012
5.000
1.000000
500.000

N EDTA = ( 0.01000 0.00012 ) N

2.3. Pha dung dch ETDA nng 5.10-4N:

Sau khi np dung dch EDTA 0.01N va mi pha long vo buret. M kha buret ly
ng 25ml dung dch ny mang i nh mc trong bnh 500ml:

0.030
0.95,V buret = z0.95 * buret = 1.96*
= 0.059
Vburet = VEDTA 0.01N

1
1
= ( 25.000 0.059 ) ml

bnh 500ml
0.050
= 1.96*
= 0.098
1
1
= ( 500.000 0.098 ) ml

0.95,V bnh 500ml = z 0.95 *

Vbnh 500ml

N EDTA 5.10-4 N =

VEDTA 0.01N *N EDTA 0.01N 25.000*0.01000

=
= 0.0005
Vbnh 500ml
500.000
2

0.95, N EDTA 5.10

-4

N
N
N

-4
-4
-4
= EDTA 5.10 N * VEDTA 0.01N + EDTA 5.10 N * NEDTA 0.01N + EDTA 5.10 N * bVnh 500ml
VEDTA 0.01N
N EDTA 0.01N
Vb nh 500ml

0.0005
0.0005
0.0005

=
*0.059 +
*0.00012 +
*0.098 = 0.0000061
25.000
0.01000
500.000

N EDTA = ( 0.0005000 0.0000061) N

EDTA phng th nghim cung cp c s dng lm cht chun gc, khng cn tin hnh
chun li. y cng l mt thun li ca phng php complexon so vi acid baz.
3. Xc nh Mg2+:
3.1. Gii thiu:

Chun mt th tch chnh xc mui Mg2+ bng dung dch complexon III trong m
amoniac pH = 10 vi ch th NET n khi mu chuyn t nho sang chm xanh chm.
3.2. Phn ng chun :
Mg + Y MgY (tt c u khng mu)
Phn ng ch th:
Xy ra khi cho d mt git complexon III:
MgIn
{ + Y MgY + {In

nho
xanh chm
C bn l mu ca MIn khc vi mu ca In3.3. Gii thch l thuyt:
/
/
Do pK MgY > pK MgIn nn phc MgIn c xu hng b phn hy to thnh phc MgY bn
hn.
Vi nng C0 gn bng 0.01, khong bc nhy pMg tnh gn ng bng 4.30 6.24 .
Ta c pMg cui 1 = pMgIn pKa + pH m = 7 11.6 + 10 = 5.4, suy ra pMg cui 2 = 5.4 + 1 = 6.4. Ta
thy ch c pMg cui 1 l nm trong khong bc nhy, vy chn pMg cui 1 kt thc chun .
Tc l mu trung gian (chm xanh chm).
3.4. Tin hnh chun :
Dng pipet ht 10.00ml dung dch kim tra cho vo erlen 250ml. Thm 10ml m pH =
10, 20mg ch th NET v chun bng EDTA 0.01M n khi dung dch chuyn t nho sang
chm xanh chm (mu trung gian). Lm thao tc tng t nh trn 3 ln v ghi li kt qu VEDTA.
3.5. S liu thc nghim:
Dung dch EDTA 0.01000N
Chun Mg2+
Ch th NET, m pH = 10
VETDA(ml)
V1
9.80

V2
9.85

3.6. Kt qu v tho lun:

0.070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VMg 2+

3
= ( 10.0000 0.0079 ) ml

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 9.817 0.072 ) ml

2
0.95, V EDTA = t0.95
*

VEDTA

N Mg 2+ =

N ETDA *VETDA 0.01000*9.817

=
= 0.009817
VMg 2+
10.0000

V3
9.80

Vtb
9.817

0.95, N Mg 2+

N 2+
N 2+
N 2+
= Mg * N ETDA + Mg * VETDA + Mg * V 2+
N ETDA
VETDA
V 2+
Mg

Mg
2

0.009817
0.009817
0.009817

=
*0.00012 +
*0.072 +
*0.0079 = 0.00014
0.01000
9.817
10.0000

N Mg 2+ = ( 0.00982 0.00014 ) N

Nguyn tc c bn quyt nh chn kt thc chun mu trung gian hay r rt

c trnh by phn gii thch l thuyt bn trn. Tc l im i mu phi nm trong khong
bc nhy, hoc ch t phi cng gn khong bc nhy cng tt. Nu pM cui 1 v c pM cui 2 u
nm lt vo trong khong bc nhy, ta u tin chn pM cui 2 hn (do mu r rt, d nhn). T
y tr v sau nu khng c gii thch g thm th vic chn mu trung gian hay r rt kt thc
chun u tun theo nguyn tc nh vy.
Khi ta cho ch th NET, mu c th m nht khc nhau ty thuc vo lng ch th s
dng. Mu sc t cam (hay cam) cho n nho m. Nu s dng lng ch th nhiu, mu
dung dch rt m th s kh khn trong vic nhn ra s i mu. V vy lm nhiu php chun
s gip ta nhn bit c lng cht ch th c s dng bao nhiu l hp l nht.
4. Xc nh cng chung ca nc my:
4.1. Gii thiu:
cng chung ca nc l do s c mt ca ca mui Mg2+ v Ca2+ tan trong nc.
c biu din bng s mili ng lng gam kim loi ( m g ) c trong mt lt nc cng.
Khng phn bit t l Mg2+: Ca2+ l bao nhiu.
Thng th nng Ca2+ c trong nc my kh thp (nh hn 0.001M). Thc nghim
cho thy php xc nh ny ch cho kt qu ng vi iu kin trong mu nc phi hin din
Mg2+. Tc l khng th xc nh ng cng ca nc my nu trong ch c mi Ca 2+. Ti
sao vy? Ion Mg2+ ng vai tr g trong php chun ny?
4.2. Phn ng chun :
CaIn + Y CaY + In
MgIn + Y MgY + In

Phn ng thay th nh lng:

CaIn + MgY CaY + MgIn

Phn ng ch th xy ra khi cho thm mt git complexon III:

MgIn + Y MgY + In

In xanh chm.
4.3. Gii thch l thuyt:
Ta chun mt th tch chnh xc ca nc my trong mi trng m pH = 10 bng
dung dch ETDA 5.10-4N vi ch th NET. Trong iu kin cc cation Ca2+ v Mg2+ ng thi
/
/
/
/
c chun v tha iu kin pK MgY = 8.24 > pK MgIn = 5.4 v pK CaY = 10.24 > pK CaIn = 3.8 . Ta
phi cho thm KCN lin kt kim loi nng c trong nc (nht l vt Cu2+), cho thm
NH2OH.HCl ngn nga s to thnh Mn (IV) trong mi trng km ph hy ch th NET.
4.4. Tin hnh chun :

Ly Vmu = 50ml nc my cho vo erlen 250ml. Thm 10ml m pH=10, ri thm 10

git KCN 10%, 10 git dung dch NH2OH.HCl 1%, lc u, thm khong 40mg ch th NET.
Tin hnh chun bng dung dch EDTA 0,01M n khi chuyn mu t nho sang xanh
chm r rt. Lm thao tc tng t nh trn 3 ln v ghi li kt qu VEDTA.
4.5. S liu thc nghim:
Dung dch EDTA 5.10-4N
Vnc my = 50ml
Ch th NET
VETDA(ml)
V1
23.90

V2
24.00

V3
23.90

Vtb
23.93

4.6. Kt qu v tho lun:

Ta xem nh Vrng = 0

0.017
0.95,V mau = z0.95 * bnh 50 ml = 1.96*
= 0.019
Vmau

3
= ( 50.000 0.019 ) ml

s
0.0577350
= 4.30*
= 0.14
3
3
= ( 23.93 0.14 ) ml

2
0.95,V ETDA = t0.95
*

VEDTA

N ETDA = ( 0.0005000 0.0000061) N

H =

VEDTA * N ETDA * 2*V1000 23.93*0.0005000* 2*1000

=
= 0.4786
Vmau
50
2

H
H
H
= * VEDTA +
* N ETDA + * Vmau
VEDTA
N ETDA
Vmau

0.4786
0.4786
0.4786

=
*0.14 +
*0.0000061 +
*0.019 = 0.011
23.93
0.0005000
50.000

H = ( 0.479 0.011) ( m g )

Vi phc CaY bn hn phc MgY vic xc nh iu kin chun ch cn xt da

trn iu kin ca pMgY. (pKCaY = 10.7, pKMgY = 8.7). Nh vy im cui ca chun l s
chuyn mu ca phc MgIn (do phc MgY km bn hn CaY nn s xy ra
CaIn + MgY CaY + MgIn ). D thy s c mt ca Mg2+ s lm cho php chun chnh xc (s
lm r vai tr ca Mg2+ hn th nghim sau).
Do nng Mg2+ v Ca2+ trong nc kh thp nn khi chun ta s dng mt lng ln
mu (50ml) ng thi s dng dung dch EDTA c nng rt thp 5.10 -4N tng th tch s
dng, lm cho phn ng chun t chnh xc cao.
V trong thnh phn ca nc cng ngoi 2 ion chnh l Mg2+ v Ca2+ cn nhiu ion kim
loi nng khc cho nn ta cn s dng cht che v cht bo v . Ion Cu2+ s to phc rt bn vi
ch th NET, lm cho khng c phn ng ch th ti im tng ng (gii phng In- nhn
bit kt thc qu trnh chun ), bng cch s dng CN- to phc bn vi ion Cu2+ s lm

mt kh nng to phc ca ion ny vi ch th. Ion Mn2+ trong mi trng baz (pH = 10), s
chuyn thnh Mn4+ ph hy ch th NET. Nn ta cho NH2OH.HCl vo nhm ngn nga s to
thnh Mn4+.
5. Xc nh cng chung ca nc cng:
5.1. Gii thiu:
Tng t nh vic xc nh cng ca nc my, nhng ta tin hnh vi loi nc
cng cng hn do phng th nghim cung cp.
5.2. Phng trnh chun :
CaIn + Y CaY + In
MgIn + Y MgY + In

Phn ng thay th nh lng:

CaIn + MgY CaY + MgIn

Phn ng ch th xy ra khi cho thm mt git complexon III:

MgIn + Y MgY + In

In xanh chm.
5.3. Cch thc tin hnh:
Ly Vmu = 5ml nc cng cho vo erlen 250ml. Thm 10ml m pH=10, 20mg ch th
NET. Tin hnh chun bng dung dch EDTA 5.10-4M n khi chuyn mu t nho sang
xanh chm r rt. Lm thao tc tng t nh trn 3 ln v ghi li kt qu VEDTA.
5.4. S liu thc nghim:
Dung dch EDTA 5.10-4N
Chun cng ca nc cng (5.00ml)
Ch th NET
VEDTA(ml)
V1
V2
26.35
26.45
5.5. Kt qu v tho lun:
Ta xem nh Vrng = 0.

0.0070
0.95,V mau = z0.95 * pipet = 1.96*
= 0.0079
Vmau

3
= ( 5.0000 0.0079 ) ml

s
0.05
= 4.30*
= 0.12
3
3
= ( 26.40 0.12 ) ml

2
0.95,V ETDA = t0.95
*

VEDTA

N ETDA = ( 0.0005000 0.0000061) N

V3
26.40

Vtb
26.40

H =

VEDTA * N ETDA * 2*V1000 26.40*0.0005000* 2*1000

=
= 5.28
Vmau
5.0000
2

H
H
H
= * VEDTA +
* N ETDA + * Vmau
VEDTA
N ETDA
Vmau

5.28
5.28
5.28

=
*0.12 +
*0.0000061 +
*0.0079 = 0.069
26.40
0.0005000
5.0000

H = ( 5.280 0.069 ) ( m g )

Tng t nh xc nh cng chung ca nc my, vic xc nh cng ca nc

cng cng da vo s c mt ca ion Mg2+.
Mt s thi im quan trong ca chun :
F = 0.99: pCa = pC0 + pDF + 2 = 5.3
F = 1.00: pCa = 0.5(pC0 + pDF + pKCaY) = 6.77
F = 1.01: pCa = pKCaY p(F1) =8.24.
Khong bc nhy pCa = 8.24 5.3 = 2.94.
Khi s dng ch th NET :
Mu trung gian: pMg = pKMgIn = 5.4 pCacui = 7.4 + p([CaY]/[MgY])
Mu r rt: pMg = pKMgIn + 1 = 6.4 pCacui = 8.4 + p([CaY]/[MgY])
Ta nhn thy pCacui ty thuc vo t l nng CaY v MgY. Nu [CaY]/[MgY] =10/1
pCa cui = 6.4 i vi im cui mu trung gian v pCa cui = 7.4 i vi im cui mu r
rt. C hai trng hp ny u c pCacui nm trong khong bc nhy 5.3 8.24 nn ta
chun cho ti khi dung dch chuyn t mu nho sang xanh chm r rt.
6. Xc nh Ca2+ nng thp khi c thm MgY:
6.1. Gii thiu:
Khi nng ban u C0 ca Ca2+ tng i ln ta c th chun Ca2+ bng complexon
III hon ton tng t nh Mg2+ bng cch dng ch th NET vi m pH = 10. Nhng khi nng
C0 ban u ca Ca2+ qu thp (c nh hn 0.001N) liu iu ny c cn chnh xc na hay
khng?
6.2. Gii thch l thuyt:
6.2.1. Kh khn khi nng ban u ca Ca2+ qu thp:
T cng thc tnh khong bc nhy ti F = 0.99: pCa = pC0 + pDF + 2, ta nhn thy khi
nng ban u C0 ca Ca2+ cng nh, im u ca khong bc nhy cng cao ln trong khi
im cui hu nh khng thay i trong cng iu kin pH tng t. iu c ngha l khong
bc nhy ngy cng b thu hp.
Mt s thi im quan trong ca chun , tnh gn ng khi nng Ca2+ vo c 0.0005
0.001N:
F = 0.99: pCa = pC0 + pDF + 2 = 5.3
F = 1.00: pCa = 0.5(pC0 + pDF + pKCaY) = 6.77
F = 1.01: pCa = pKCaY p(F1) = 8.24
Tc l cn phi c mt cht ch th sao cho 5.30 < pCacui < 8.24. R rng l khi dng NET, pH =
10 th ngay c trng hp chun n mu chm r rt cng ch c th t gi tr pCa cui =

%
4.80. Ta tnh c khi In ,Ca =

104.8 *100
= 3.2% . im bt li na l phc mu CaIn vi
C0

NET c mu lt nn kh nhn ra im cui chun .

6.2.2. Gii php thm MgY:
Tnh hung tr nn rt thun li khi ta thm vo erlen mt lng thch hp MgY, s
chuyn mu rt r rt, kt qu chun s ng. Gii thch bng s chuyn dch ng k ca cn
bng phn ng:
CaIn + MgY CaY + MgIn (1)
5.4
8.7
7.0
10.7
pK cn bng = 17.7 14.1 = 3.6
Khi Ca2+ chun ht, th vi mt git complexon III d lp tc xy ra phn ng ch th sau:
MgIn + Y MgY + In (2)
nho
Xanh chm
Lng MgY mt i trong phn ng (1) (trc im tng ng) c hon tr trong
phn ng (2) (sau im tng ng).
6.3. Tin hnh chun :
6.3.1. iu ch MgY2- ngay trong dung dch chun :
Ly 2ml dung dch Mg2+ 10-3M cho vo erlen 250ml, 10ml m amoniac pH=10, 20mg
ch th NET, lc u cho tan. Chun bng dung dch EDTA 5.10-4M n chuyn mu r rt t
nho sang xanh chm. Ghi th tch VI. Tuyt i khng cho d EDTA.
6.3.2. Xc nh Ca2+:
Np thm dung dch EDTA n vch 0 ca buret. Ly 10.00ml dung dch cha Ca 2+ vo
erlen va iu ch MgY2-. Mu dung dch lp tc bin tr li nho, chun cho ti khi
chuyn sang mu xanh chm, ghi th tch VII tiu hao cho chun Ca2+. Lm li 3 ln ghi cc
gi tr trung bnh ca VI v VII.
6.4. S liu thc nghim:
Dung dch EDTA 5.10-4N
iu ch MgY2Ch th NET
VY(1)(ml)
V1
7.30

V2
7.30

V3
7.40

Vtb
7.33

Dung dch EDTA 5.10-4N

Chun Ca2+
Ch th NET
VY(2)(ml)
V1
9.40

V2
9.45

V3
9.35

Vtb
9.40

6.5. Kt qu v tho lun:

NY * 40.08 0.0005000* 40.08

=
= 0.000020
1000
1000

TY / Ca 2+ =

0.95T

Y / Ca 2+

40.08 TY / Ca2+
=
* NY = 0.0000000090

1000 NY

TY / Ca 2+ = ( 0.0000200000 0.0000000090 ) g / ml

V pipet = ( 10.0000 0.0079 ) ml

s
0.05
= 4.30 *
= 0.12
3
3
= ( 9.40 0.12 ) ml

2
0.95VEDTA 2 = t0.95
*

VEDTA 2

bnh 100ml
0.025
= 1.96*
= 0.049
1
1
= ( 100.000 0.049 ) ml

0.95,V bnh 100ml = z0.95 *

Vbnh 100ml
mCa 2+ =

Ca 2+

TY / Ca 2+ *VEDTA 2 *Vbnh 100ml

V pipet

m 2+
= Ca * T 2+
T 2+
Y / Ca
Y / Ca

0.000020*9.40*100.000
= 0.00188
10.000
2

mCa 2+

m 2+
mCa 2+
* T
+
* Vbnh 100ml + Ca * V pipet
+

Y /Ca 2+
Vbnh 100ml
Vpipet

VEDTA 2
2

0.00188
0.00188
0.00188
0.00188

=
*9.0*109 +
*0.12 +
*0.049 +
*0.0079
5
2.00000*10
9.40
100.000
10.0000

mCa2+ = ( 0.00188 0.00002 ) g

[V ]
7.33
Ca 2+ = 108.4 * EDTA 1 = 108.4 *
= 3.1*109
9.40
[V
]
ETDA 2

pCa 2+ = 8.508638
2

Ca 2+
Ca 2 +

= 7.1217 *10 11
Ca2+ =
* VEDTA 1 +
VEDTA 2
VEDTA 1
VEDTA 2

Ca 2+
7.1217 *1011
p Ca2+ = p Ca 2+ * 2+ = 8.508638*
= 0.20

3.1*109
Ca
pCa 2+ = 8.50 0.20

Vy vic thm MgY khng h nh hng n t l hp thc ca phn ng chun Ca2+

nhng li gy mt hiu ng mu c li: mn mu ca MgIn (mu kh mnh) chun
Ca2+ v lm cho s chuyn mu ng thi im Fcui cn thit.
Nguyn tc iu chnh lng MgY thm vo erlen:

[ Y ] C = K MgY *

[ MgY ] C
[ Mg ] C

[ Ca ] C = [ Mg ] C *

= K CaY *

[ CaY ] C
[ Ca ] C

K CaY [ CaY ] C
*
K MgY [ MgY ] C

[ Ca ] C = 106.4 *

C
1010.7 CCa
*
= 108.4 * Ca
8.7
10
CMgY
CMgY

pCaC = 8.4 + p

CCa
CMgY

5.30 < pCacui < 8.24 th 10

3.1

<

CMgY
CCa

< 1.5 . V d khi CCa = 10CMgY th pCa cui = 8.4 1 = 7.4

nm gia 5.30 v 8.24.

Ngoi ra gii php thm MgY cn c th p dng chun Ba2+ vi ch th NET ti pH
= 10. Nh vy, cng vi vic iu chnh lng MgY khng ch s chuyn mu din ra thi
im mong mun, vai tr ca ion Mg2+ trong hai th nghim xc nh cng ca nc trn
cng c lm sng t.
7. Xc nh Ca2+ nng thp khi khng c MgY:
7.1. Gii thiu:
Ta i xc nh li Ca2+ nng thp khi khng c MgY so snh vi kt qu ca th
nghim trn.
7.2. Phn ng chun :
Ca + Y CaY
Phn ng ch th:
CaIn + Y CaY + In
nht
Xanh chm
7.3. Cch thc tin hnh:
Ly 10.00ml dung dch Ca2+ cn chun cho vo erlen. Thm 10.00ml dung dch m
pH = 10, ch th NET. Tin hnh chun bng dung dch ETDA 5.10-4N cho n khi c s i
mu t nht sang xanh chm. Lp li th nghim 3 ln, ghi kt qu ca VEDTA trung bnh.
7.4. S liu thc nghim:
Dung dch EDTA 5.10-4N
Chun Ca2+
Ch th NET
VY(ml)
V1
12.80
7.5. Kt qu v tho lun:

V2
12.70

V3
12.75

Vtb
12.75

 0.95,V pipet = z0.95 *

VCa2+

pipet

= 1.96*

3
= ( 10.0000 0.0079 ) m l

0.0070
= 0.0079
3

N EDTA = ( 0.0005000 0.0000061) N

s
0.05
= 4.30*
= 0.12
3
3
= ( 12.75 0.12 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Ca 2+ =

N ETDA *VETDA 0.0005000*12.75

=
= 0.0006375
VCa 2+
10.0000
2

0.95, N Ca2+

N 2+
N 2+
N 2+
= Ca * N ETDA + Ca * V ETDA + Ca * V 2+
Ca
N ETDA
VETDA
VCa2+
2

0.0006375
0.0006375
0.0006375

=
*0.0000061 +
*0.12 +
*0.0079 = 0.0000098
0.0005000
12.75
10.0000

N Ca 2+ = ( 0.0006375 0.0000098) N

Thao tc chun v x l s liu n gin hn nhiu th nghim trn nhng chc chn
kt qu s khng chnh xc bng do kh nhn ra im i mu (CaIn- kh nht) v im cui c
th nm ngoi khong bc nhy.
Trn thc t m bo c mt kt qu ng khi nng Ca 2+ kh thp (vo c khong
di 0.001 n 0.0005N) ngi ta thng chn cch thm mt lng nht nh MgY vo erlen.
8. Xc nh Ca2+ nng cao:
8.1. Gii thiu:
Khi nng ban u C0 ca Ca2+ tng i ln ta c th chun Ca2+ bng complexon
III hon ton tng t nh Mg2+ bng cch dng ch th NET vi m pH = 10.
8.2. Phn ng chun :
Ca + Y CaY
Phn ng ch th:
CaIn + Y CaY + In
nho
Xanh chm
8.3. Cch thc tin hnh:
Ly 10.00ml dung dch Ca2+ cn chun cho vo erlen. Thm 10.00ml dung dch m pH = 10,
ch th NET. Tin hnh chun bng dung dch ETDA 0.01N cho n khi c s i mu t
nho sang xanh chm. Lp li th nghim 3 ln, ghi kt qu ca VEDTA trung bnh.
8.4. S liu thc nghim:
Dung dch EDTA 0.01000N
Chun Ca2+
Ch th NET
VY(ml)
V1

V2

V3

Vtb

9.80

9.85

9.85

9.833

8.5. Kt qu v tho lun:

0.0070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VCa2+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 9.833 0.072 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Ca2+ =

N ETDA *VETDA 0.01000*9.633

=
= 0.009833
VCa2+
10.0000
2

0.95, N Ca 2+

N 2+
N 2+
N 2+
= Ca * N ETDA + Ca * VETDA + Ca * V 2+
Ca
N ETDA
VETDA
VCa2+
2

0.009833
0.009833
0.009833

=
*0.00012 +
*0.072 +
*0.0079 = 0.00014
0.01000
9.833
10.0000

N Ca2+ = ( 0.00983 0.00014 ) N

Tng t nh khi chun Mg2+

Trn thc t nng Ca2+ do phng th nghim chun b vo khong 0.01N. Kt qu nng
2+
Ca xc nh c 0.00983N l c th chp nhn c.
Lu lng ch th NET thm vo phi va d dng nht quan st c s chuyn
mu ca dung dch trong erlen.
9. Xc nh Cu2+ vi ch th MUREXID
9.1. Gii thiu:
Chun Cu2+ bng dung dch ETDA 0.01N vi ch th MUR trong mi trng m
amoniac c pH = 8.
9.2. Phn ng chun :
Cu ( NH 3 ) 24+ + Y CuY + NH 3

Phn ng ch th:

CuIn + Y CuY + In

Cam
Tm hng
Hin tng: c s chuyn mu dung dch t vng cam sang tm hng.
9.3. Gii thch l thuyt:
Vi CCu = 0.01M. Ti pH = 8, pY(H) = 2.3, pH2In = 4.1, pCu(NH3) = 3.5 ([NH3] = 102.25
), pKCuY = 18.8
pKCuY = 18.8 2.3 3.5 = 13.0.
Tnh gn ng bc nhy pCu = 7.8 14.5.
Ta tm c: pCucui = pKCuIn + 1 = 15.0 4.1 3.5 + 1 = 8.4.
2+

9.4. Cch thc tin hnh:

Ly 10.00ml dung dch cho vo erlen 250ml. Thm 1ml NH4Cl 1M, 20mg hn hp ch th
MUREXID, lc u. Thm t t tng git dung dch NH3 1M n khi xut hin mu vng (hoc
vng lc ty nng Cu2+). Sau chun bng dung dch EDTA 0.01M n khi dung dch
chuyn mu t vng sang tm hng. Lp li thao tc nh trn 3 ln v ly gi tr VEDTA trung bnh.
9.5. S liu thc nghim:
Dung dch EDTA 0.01000N
Ch th MUR
Chun Cu2+
VY(ml)
V1
10.15

V2
10.10

V3
10.15

Vtb
10.133

9.6. Kt qu v tho lun:

0.070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VCu 2+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 10.133 0.072 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Cu 2+ =

N ETDA *VETDA 0.01000*10.133

=
= 0.010133
VCu 2+
10.0000

0.95, N Cu 2+

2
2
N Cu 2+
N Cu 2+
N Cu 2+
=
* N ETDA +
* VETDA +
* V 2+
Mg
N ETDA
VETDA
VMg 2+
2

0.010133
0.010133
0.010133

=
*0.00012 +
*0.072 +
*0.0079 = 0.00014
0.01000
10.133
10.0000

N Cu 2+ = ( 0.01013 0.00014 ) N

Nhng tnh ton trn cho thy php chun Cu 2 + cn phi thc hin ti pH khng c
qu cao v nng NH 3 t do khng c qu ln v phc amiacat ca Cu 2 + kh bn.
D nhin m NH 3 khng tht tt pH = 8.V vy cn phi kt hp iu chnh pH cn c
theo s xut hin mu vng da cam ca phc CuH 2 In' . S c mt NH4Cl ngn cn s tng vt
pH bng cch cho thm NH3.
Php chun t chnh xc kh tt.
Dung dch khi cho NH3 vo thong c l kt ta ca hydroxide ng.
10. Xc nh Pb2+ vi ch th NET bng phng php chun thay th:
10.1. Gii thiu:

Phng php chun thay th: Cho cht nh phn X tc dng vi MY to thnh MX v
gii phng ra Y. Tin hnh chun Y, sau da vo nng v liu lng thuc th tnh
ra nng cht X.
10.2. Phn ng chun :
Dung dch MgY c iu ch sn. Khi cho thm Pb2+ vo lp tc xy ra phn ng thay
th nh lng:
Pb + MgY PbY + Mg
Chun Mg2+ va c gii phng ra bng dung dch EDTA 0.01000N. Xy ra phn
ng ch th:
MgIn + Y MgY + In
Hin tng: c s chuyn mu t nho sang chm xanh chm (xem gii thch c th
phn chun Mg2+).
10.3. Gii thch l thuyt:
Phc PbY bn hn rt nhiu so vi phc MgY (pKPbY = 18.04, pKMgY = 8.70) nn u tin
to thnh phc PbY. Ta mn mu ca MgIn chun Pb2+ do s chuyn mu ca MgIn
(mu kh mnh) sang chm xanh chm tng i d nhn.
10.4. Cch thc tin hnh:
iu ch MgY2-: Cho 1,8ml Mg2+ vo erlen, im pH = 10, ch th NET. Cho t t dung
dch EDTA 0.01000N trn buret vo n thy mu chm xanh chm th ngng, khng c cho
d EDTA. Ly 10.00ml mu vo erlen, lc k cho phn ng hon ton, sau chun cho n khi
c mu chm xanh chm. V y l phn ng chun thay th Pb2+ bng Mg2+, nn nh phn
ng 1, ta chun n mu trung gian l ngng phn ng chun .
10.5. S liu thc nghim:
Dung dch EDTA 0.01000N
Ch th NET
Chun Pb2+
VY(ml)
V1
9.70
10.6. Kt qu v tho lun:

0.070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VPb2+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 9.667 0.072 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Pb2+ =

N ETDA *VETDA 0.01000*9.667

=
= 0.009667
VPb2+
10.0000

V2
9.65

V3
9.65

Vtb
9.667

 0.95, N Pb2+

2
2
N Pb2+
N Pb2+
N Pb2+
=
* N ETDA +
* VETDA +
* V 2+
Mg
N ETDA
VETDA
VMg 2+
2

0.009667
0.009667
0.009667

=
*0.00012 +
*0.072 +
*0.0079 = 0.00014
0.01000
9.667
10.0000

N Pb2+ = ( 0.00967 0.00014 ) N

chnh xc kh cao: 1

P ,C Pb2+
CPb2+

= 1

0.00014
= 0.9855 = 98.55%
0.00967

Bi thc tp ny lc u phi iu ch MgY2- nn cn phi rt cn thn, khng c cho

d EDTA, nu cho d th s mc sai s tha.
Lc k cho phn ng gia dung dch Pb2+ v MgY 2 xy ra hon ton. Lng MgY iu
ch lc no cng phi d so vi Pb2+ m bo phn ng thay th nh lng xy ra n tn
cng (khng cn Pb2+ trong dung dch).
Khi kt thc chun , nu nghi ng mu ca dung dch, ghi li s liu va dng chun
, thm vi git EDTA kim tra li. Nu dung dch i sang mu chm r rt ri dng chuyn
mu th s liu va ghi chnh xc.
11. Xc nh Pb2+ vi ch th XO bng phng php chun trc tip:
11.1. Gii thiu:
Chun gin tip i khi gp nhiu kh khn do khu iu ch MgY i hi s chnh
xc kh cao. C th la chn con ng chun trc tip bng ch th XO trong mi trng
m axetat pH = 5. y ta s so snh ng ca hai phng php chun ny.
11.2. Phn ng chun :
Pb + Y PbY
Phn ng ch th:
PbIn + Y PbY + In
tm
Vng chanh
C bn l mu ca PbIn v In- khc nhau.
Hin tng: C s chuyn mu dung dch t tm sang vng chanh.
11.3. Cch thc tin hnh:
Ht 10.00ml dung dch kim tra cho vo erlen 250ml, 5ml m axcetat pH = 5, 1-2 git
ch th XO pha sn. Chun bng dung dch complexon III 0.01000N cho n khi c s chuyn
mu t tm sang vng chanh. Lm 3 ln, ly kt qu trung bnh.
11.4. S liu thc nghim:
Dung dch EDTA 0.01000N
Ch th XO
Chun Pb2+
VY(ml)
V1
9.65

V2
9.60

V3
9.65

Vtb
9.633

11.5. Kt qu v tho lun:

0.070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VPb2+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 9.633 0.072 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Pb2+ =

N ETDA *VETDA 0.01000*9.633

=
= 0.009633
VPb2+
10.0000

0.95, N Pb2+

2
2
N Pb2+
N Pb2+
N Pb2+
=
* N ETDA +
* VETDA +
* V 2+
Mg
N ETDA
VETDA
VMg 2+
2

0.009633
0.009633
0.009633

=
*0.00012 +
*0.072 +
*0.0079 = 0.00014
0.01000
9.633
10.0000

N Pb2+ = ( 0.00963 0.00014 ) N

chnh xc kh cao: 1

P ,C Pb2+
CPb2+

= 1

0.00014
= 0.9854 = 98.54%
0.00963

Kt qu ca hai phng php chun Pb2+ l gn bng nhau. Cho thy hai phng php
u c th xc nh hm lng ca ion ch.
T tm chuyn sang vng chanh khng d quan st, thng rt d sai s tha v xc
nh sai mu vng chanh.
12. Xc nh nng Zn2+ theo dung dch chun EDTA 0.01000N:
12.1. Gii thiu:
Cn xc nh chnh xc nng dung dch km khong 0.01N dng chun ngc
cho cc th nghim v sau.
12.2. Phn ng chun :
Zn + Y ZnY
Phn ng ch th:
In + Zn ZnIn
Vng chanh
Hng tm
12.3. Cch thc tin hnh:
Np dung dch Zn2+ vo buret. T buret cha EDTA 0.01000N ly 10.00 dung dch EDTA
cho vo erlen. Thm 5ml dung dch m acetat pH = 5, 1-2 git ch th XO pha sn, lc u.
Chun bng dung dch Zn2+ n khi c s chuyn mu t vng chanh sang hng tm. Lm 3
ln, ghi th tch trung bnh.
12.4. S liu thc nghim:

Dung dch ETDA 0.01000N

Ch th XO
Xc nh Zn2+
VEDTA(ml)
V1
8.75

V2
8.80

V3
8.85

Vtb
8.80

12.5. Kt qu v tho lun:

0.030
0.95,V buret = z0.95 * buret = 1.96*
= 0.034
VEDTA

3
= ( 10.000 0.034 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.05
= 4.30*
= 0.12
3
3
= ( 8.80 0.12 ) ml

2
0.95,V Zn2+ = t0.95
*

VZn2+

N Zn2+ =

N ETDA *VETDA 0.01000*10.000

=
= 0.011364
VZn2+
8.80
2

0.95, N Zn2+

N 2+
N 2+
N 2+
= Zn * N ETDA + Zn * VETDA + Zn * V 2+
Zn
N ETDA
VETDA
VZn2+
2

0.011364
0.011364
0.011364

=
*0.00012 +
*0.034 +
*0.12 = 0.00021
0.01000
10.000
8.80

N Zn2+ = ( 0.01136 0.00021) N

Nng km nh hng kt qu ca cc th nghim v sau, do cn thn trng khi

chun trnh xc nh sai nng Zn2+.
13. Xc nh hn hp Fe3+ v Al3+ vi ch th XO bng phng php chun ngc:
13.1. Gii thiu:
Phng php chun ngc: Cho cht nh phn M tc dng vi lng d chnh xc
ca cht Y to ra MY. Dng N chun lng Y cn d trong dung dch. Da no nng , liu
lng ca N xc nh cht M.
13.2. Phn ng chun :
Fe3+ v Al3+ cng to phc vi Y:
Fe + Y FeY
Al + Y AlY
Phn ng chun ngc:
Zn + Y ZnY
Phn ng ch th:
In + Zn ZnIn
In- c mu vng chanh, ZnIn mu hng tm.

Hin tng: C s chuyn mu dung dch t vng chanh sang hng tm.
13.3. Gii thch l thuyt:
Zn2+ ch to phc vi lng Y cn d ch khng ph hy c phc FeY v AlY
to ra t trc do pKAlY > pKZnY v pKFeY > pKZnY. y cng l iu kin chun ngc ni
chung.
13.4. Cch thc tin hnh:
T buret x mt lng d chnh xc dung dch EDTA 0.01000N (25ml) vo erlen,
thm vo 10.00ml hn hp kim tra, 5ml m acetat pH = 5, lc u. un si 2 pht phn ng
to phc ca Fe3+ v Al3+ xy ra hon ton. ngui, thm hai git ch th XO ri chun bng
dung dch km (va xc nh trn) cho n khi chuyn mu t vng chanh sang hng tm. Lm
3 ln, ghi kt qu trung bnh VI.
13.5. S liu thc nghim:
Dung dch Zn2+ 0.01136N
Ch th XO
Xc nh tng Al3+ + Fe3+
V(I)Zn(ml)
V1
14.55

V2
14.60

V3
14.65

13.6. Kt qu v tho lun:

0.0070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
3
Vhh = ( 10.0000 0.0079 ) ml

N EDTA = ( 0.01000 0.00012 ) N

buret
0.030
= 1.96*
= 0.034
3
3
= ( 25.000 0.034 ) ml

0.95,V buret = z0.95 *

V EDTA

s
0.05
= 4.30*
= 0.12
3
3
= ( 14.60 0.12 ) ml

2
0.95,V Zn2+ = t0.95
*

VZn2+

N Zn2+ = ( 0.01136 0.00021) N

VEDTA =

N Zn2+ *VZn2+

0.95,V EDTA

N EDTA

0.01136*14.60
= 16.5856
0.01000

V
= EDTA * N 2+
N 2+
Zn
Zn

VEDTA
* V 2+
+
Zn
V
2+
Zn

VEDTA

* N EDTA
+

N EDTA

Vtb
14.60

16.5856
16.5856
16.5856

=
*0.00021 +
*0.12 +
*0.00012 = 0.39
0.01136
14.60
0.01000

VEDTA = ( 16.59 0.39 ) ml

VEDTA = V EDTA VEDTA = 25.000 16.59 = 8.41

0.95,V EDTA = V2 EDTA + V2 EDTA = 0.0342 + 0.392 = 0.39

VEDTA = ( 8.41 0.39 ) ml
N hh =

N EDTA * VEDTA 0.01000*8.41

=
= 0.00841
Vhh
10.0000
2

0.95, N hh

N
N hh
N
= hh * N EDTA +
* VEDTA + hh * Vhh
N EDTA
VEDTA
Vhh

0.00841
0.00841
0.00841

=
*0.00012 +
*0.39 +
*0.0079 = 0.00040
0.01000
8.41
10.0000

N hh = ( 0.00841 0.00040 ) N

Trong iu kin tin hnh chun l pH = 5, ta c Al3+ v Fe3+ tn ti di dng phc

hydroxo, mun chun trc tip phi tin hnh ph v phc hydroxo phn ng vi EDTA
xy ra rt chm, khng ph hp yu cu ca mt phn ng chun nn khng th chun
trc tip. Nn chun ngc trong trng hp ny c xem nh gii php ti u. Vic un
nng hn hp sau khi cho mt lng d chnh xc EDTA nhm cho phn ng xy ra nhanh
hn v hon ton.
Thc t chun ngc ch c th thc hin c mt khi tha iu kin: Phc ca kim
loi cho vo chun lng EDTA cn d phi km bn hn so vi phc ca kim loi to
thnh vi EDTA trc . Ngha l trong phn gii thiu trn pKMY phi ln hn pKNY.
14. Xc nh ring Al3+ t hn hp bng phng php chun ngc:
14.1. Gii thiu:
Do Al3+ to phc vi EDTA, nn chun lng Al3+ cn phi tin hnh php
chun thay th. Bng vic cho NaF vo Al3+ to phc vi F- bn hn phc to vi EDTA.
Lng EDTA gii phng ra ng bng lng Al3+ s c chun bi dung dch Zn2+.
14.2. Phn ng chun :
AlY + 6F AlF6 + Y
Zn + Y ZnY
Phn ng ch th:
In + Zn ZnIn
Hin tng: c s chuyn mu t vng chanh sang hng tm.
14.3. Cch thc tin hnh:
Tip tc thm 5ml dung dch bo no NaF. un si 2 pht phn ng to phc AlF6 xy
ra hon ton. ngui, thm 1-2 git ch th XO, lc u. Chun bng dung dch km
0.01136N n khi chuyn mu t vng chanh sang hng tm. Lm 3 ln, ghi li kt qu trung
bnh VII.

14.4. S liu thc nghim:

Dung dch Zn+ 0.01136N
Ch th XO
Xc nh Al3+
V(II)Zn(ml)
V1
3.70

V2
3.60

V3
3.65

Vtb
3.65

14.5. Kt qu v tho lun:

s
0.05
= 4.30*
= 0.12
3
3
= ( 3.65 0.12 ) ml

2
0.95,V Zn2+ = t0.95
*

VZn2+

N Zn2+ = ( 0.01136 0.00021) N

0.95,V pipet = z0.95 *

pipet

= 1.96*

3
Vhh = ( 10.0000 0.0079 ) m l
N Al 3+ =

N Zn2+ *VZn2+

0.95, N Al 3+

Vhh

0.0070
= 0.0079
3

0.01136*3.65
= 0.0041464
10.0000

N 3+
= Al * N 2+
N 2+
Zn
Zn

N Al 3+
* V 2+
+
Zn
V
2+
Zn
2

N Al 3+

* Vhh
+

Vhh
2

0.0041464
0.0041464
0.0041464

=
*0.00021 +
*0.12 +
*0.0079 = 0.00016
0.01136
3.65
10.0000

N Al 3+ = ( 0.00415 0.00016 ) N

Vic un nng s gip cho phn ng thay th xy ra nhanh v hon ton hn. Vic cho
cht ch th vo hn hp nh phn thc hin nhit thng, tr trng hp bt buc mi tin
hnh s dng ch th khi hn hp nng. Nhit cao c th ph hy ch th.
T hai th nghim trn, ta thy rng khi chun ring Al3+ hay hn hp c Al3+ trong
thng phi dng phng php chun ngc. Do nhm c kh nng to phc hidroxo kh
bn, s kh to phc vi EDTA, khng ph hp vi phng php chun trc tip. Mc khc,
c th do khong bc nhy ca Al3+ kh hp hoc khng c mt ch th no c kh nng to ra
pAl cui nm trong khong bc nhy nn nhn chung chun Al3+ bng phng php trc tip
l mt iu ht sc kh khn. Trong trng hp ny, chun ngc hay chun thay th l
phng php ti u nht.
15. Xc nh ring Fe3+ bng EDTA 0.01000N vi ch th SSA:
15.1. Gii thiu:
Ta thc hin chun ring Fe3+ ri so snh vi cch chun chung hn hp Al3+ + Fe3+
pha trn so snh s sai khc gia hai cch lm ny.
15.2. Cch thc tin hnh:

Ly 10.00ml mu, thm NH3 v lc mnh n thong c th cho thm vi git HCl cho
tan, muc ch ca vic ny l nhm trung ha lng acid d trong dung dch mu. Hai ion kim
loai ny dc pha trong mi trng acid nn cn trung ha li. Lc u ta cho thm NH 3 n
thy thong c th dung dch d mt t NH3, do ta phi thm vi git HCl a v pH
bng 7 (thy dung dch trong tr li). Nhng phn ng chun ny phi c thc hin pH =
2, nn ta tip tc cho thm 1ml HCl 1: 2 a pH v gn bng 2. Dung dch phi c un
nng n 700C phn ng chun xy ra tc thi. Tip theo ta thm ch th SSA v chun
dung dch ngay lc cn nng phn ng chun xy nhanh v c tnh nh lng.
Hin tng: Dung dch chuyn mu t hng tm sang vng nht hoc khng mu.
15.3. S liu thc nghim:
Dung dch EDTA 0.01000N
Ch th SSA
Xc nh Fe3+
VY(ml)
V1
4.90

V2
4.95

V3
4.90

15.4. Kt qu v tho lun:

0.0070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VFe3+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 4.917 0.072 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Fe3+ =

N ETDA *VETDA 0.01000* 4.917

=
= 0.004917
VFe3+
10.0000
2

0.95, N Fe3+

N 3+
N 3+
N 3+
= Fe * N ETDA + Fe * VETDA + Fe * V 3+
Fe
N ETDA
VETDA
VFe3+
2

0.004917
0.004917
0.004917

=
*0.00012 +
*0.072 +
*0.0079 = 0.000093
0.01000
4.917
10.0000

N Fe3+ = ( 0.004917 0.000093) N

chnh xc: 98.11%

So snh vi cch tnh ra nng Fe3+ t nng tng:
N Fe3+ = N hh N Al 3+ = 0.00841 0.00415 = 0.00426

0.95, N Fe3+ = N2 hh + N2

Al 3+

= 0.000402 + 0.000162 = 0.00043

N Fe3+ = ( 0.00426 + 0.00043) N

chnh xc: 89.91%

Vtb
4.917

Kt qu thu c t hai phng php sai khc nhau kh nhiu nn kh c th kt lun

phng php no ti u hn. Tuy nhin theo nhn xt ch quan, cch xc nh nng Fe3+ theo
phng php trc tip s c ng cao hn do trnh c nhiu giai on trung gian phc tp.
Trong iu kin chun , Al3+ khng b chun bi EDTA v khng cn tr php
'
'
'
'
xc nh Fe3+ do pH = 2 pK FeY = 11.44 , pK AlY = 1.69 v pK FeIn = 14.05 pK In ( H ) = 3.72 ,
'
pK AlIn
= 13.20 pK In' ( H ) = 2.87 . Gi s CFe3+ = 0.01M , khong bc nhy tnh gn ng s l
4.30 9.44 . Do pFe cui = 3.72 + 1 = 4.72.
'
pK AlIn

Ngoi ra, ch th SSA cn c tc dng che Al3+ khi tin hnh chun trc tip Fe3+ (
= 13.20 pK In' ( H ) = 2.87 ti pH = 2). Do c tnh chn lc rt cao.

PHN KIM TRA

L thuyt v thao tc tin hnh: nh trn.
Sinh vin: T Kim Cn
MSSV: 1014021
MS dung dch kim tra: 07
1. S liu thc nghim:
Dung dch EDTA 0.01000N
Ch th SSA
Xc nh Fe3+
VY(ml)
V1
13.05

V2
13.10

V3
13.00

2. Kt qu x l s liu:

0.0070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VFe3+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.05
= 4.30*
= 0.12
3
3
= ( 13.05 0.12 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Fe3+ =

N ETDA *VETDA 0.01000*13.05

=
= 0.01305
VFe3+
10.0000
2

0.95, N Fe3+

N 3+
N 3+
N 3+
= Fe * N ETDA + Fe * VETDA + Fe * V 3+
Fe
N ETDA
VETDA
VFe3+

Vtb
13.05

0.01305
0.01305
0.01305

=
*0.00012 +
*0.12 +
*0.0079 = 0.00020
0.01000
13.05
10.0000

N Fe3+ = ( 0.01305 0.00020 ) N

Sinh vin: L Ngc Cng

MSSV: 1014029
MS dung dch kim tra: 65
1. S liu thc nghim:
Dung dch EDTA 0.01000N
Ch th SSA
Xc nh Fe3+
VY(ml)
V1
10.90

V2
10.85

V3
10.90

2. Kt qu x l s liu:

0.0070
0.95,V pipet = z0.95 * pipet = 1.96*
= 0.0079
VFe3+

3
= ( 10.0000 0.0079 ) m l

N EDTA = ( 0.01000 0.00012 ) N

s
0.0288675
= 4.30*
= 0.072
3
3
= ( 10.883 0.072 ) ml

2
0.95,V EDTA = t0.95
*

VEDTA

N Fe3+ =

N ETDA *VETDA 0.01000*10.883

=
= 0.010883
VFe3+
10.0000
2

0.95, N Fe3+

N 3+
N 3+
N 3+
= Fe * N ETDA + Fe * VETDA + Fe * V 3+
Fe
N ETDA
VETDA
VFe3+
2

0.010883
0.010883
0.010883

=
*0.00012 +
*0.072 +
*0.0079 = 0.00015
0.01000
10.883
10.0000

N Fe3+ = ( 0.01088 0.00015 ) N

Vtb
10.883