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  • Dox Broth

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    Czapek Dox Broth is a semisynthetic medium used for cultivating fungi. It contains sucrose as the sole carbon source and sodium nitrate as the sole nitrogen source, as well as dipotassium phosphate, magnesium sulfate, potassium chloride, and ferrous sulfate. To prepare, suspend 35.01 grams of the powder in 1000 ml of distilled water, heat to dissolve completely, then autoclave at 15 lbs pressure for 15 minutes. When inoculated with various fungi like Aspergillus brasiliensis, Candida albicans, and Saccharomyces cerevisiae, good to luxuriant growth is observed.

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    dox broth

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    Czapek Dox Broth is a semisynthetic medium used for cultivating fungi. It contains sucrose as the sole carbon source and sodium nitrate as the sole nitrogen source, as well as dipotassium phosphate, magnesium sulfate, potassium chloride, and ferrous sulfate. To prepare, suspend 35.01 grams of the powder in 1000 ml of distilled water, heat to dissolve completely, then autoclave at 15 lbs pressure for 15 minutes. When inoculated with various fungi like Aspergillus brasiliensis, Candida albicans, and Saccharomyces cerevisiae, good to luxuriant growth is observed.

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    19 views1 page

    Dox Broth

    Uploaded by

    johnnash916
    Czapek Dox Broth is a semisynthetic medium used for cultivating fungi. It contains sucrose as the sole carbon source and sodium nitrate as the sole nitrogen source, as well as dipotassium phosphate, magnesium sulfate, potassium chloride, and ferrous sulfate. To prepare, suspend 35.01 grams of the powder in 1000 ml of distilled water, heat to dissolve completely, then autoclave at 15 lbs pressure for 15 minutes. When inoculated with various fungi like Aspergillus brasiliensis, Candida albicans, and Saccharomyces cerevisiae, good to luxuriant growth is observed.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
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    HiMedia Laboratories

    Technical Data

    Czapek Dox Broth


    Czapek Dox Broth is a semisynthetic medium used for the general cultivation of fungi. Composition** Ingredients Gms / Litre Sucrose 30.000 Sodium nitrate 3.000 Dipotassium phosphate 1.000 Magnesium sulphate 0.500 Potassium chloride 0.500 Ferrous sulphate 0.010 Final pH ( at 25C) 7.30.2 **Formula adjusted, standardized to suit performance parameters

    M076

    Directions
    Suspend 35.01 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Mix well and dispense as desired.

    Principle And Interpretation


    Fungi, including yeasts and filamentous species or moulds are ubiquitously distributed in nature. Czapek Dox Broth is a semi-synthetic medium used for the cultivation of fungi, containing sodium nitrate as the sole source of nitrogen. This medium is prepared according to the formula developed by Thom and Church (1), which has a defined chemical composition. Czapek Dox Broth is the modification of the original medium of Czapek Dox (2, 3) as per Thomas and Raper (4). Sucrose serves as the sole source of carbon while sodium nitrate serves as the sole source of nitrogen. Dipotassium phosphate buffers the medium. Magnesium sulphate, potassium chloride, ferrous sulphate serves as sources of essential ions.

    Quality Control
    Appearance Cream to yellow homogeneous free flowing powder Colour and Clarity of prepared medium Light yellow coloured, clear to slightly opalescent solution in tubes Reaction Reaction of 3.5% w/v aqueous solution at 25C. pH : 7.30.2 Cultural Response M076: Cultural characteristics observed after an incubation at 25-30C for 48-72 hours. Organism Inoculum Growth (CFU) Aspergillus brasiliensis 50-100 goodATCC 16404 luxuriant Candida albicans ATCC 50-100 good10231 luxuriant Saccharomyces cerevisiae 50-100 goodATCC 9763 luxuriant Reference 1. Thom and Church, 1926, The Aspergilli, 39. 2. Czapek, 1920-1903, Bcitr. Chem. Physiol. Pathol., 1:540 3. Dox, 1910, U.S. Dept. of Agr. Bur. Anim. Ind. Bull., 120:70 4. Thom and Raper, 1945, Manual of Aspergilli, 39. Storage and Shelf Life Store below 30C and the prepared medium at 2 - 8C. Use before expiry date on the label.

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