V. RESULTS Observation 1. Fermentation tube A demonstrates the effect of sterilization. The appearance of the medium in tube A is somewhat cloudy.

2. After adding live yeast suspension to fermentation tube B and leaving it for two days, the medium in the tube B shows accumulation of the yeast at the bottom of the fermentation tube. When compared to fermentation tube A, tube B is somewhat clearer and has more accumulation formed at the bottom than tube A. 3. The medium in the arm of fermentation tube C after five days also shows accumulation of the yeast at the bottom of the tube. The only difference is that 4. After removing the plug off the mouth of tube C, what substance did you smell out of the odor? 5. Result of procedure 6 to prove the presence of Carbon dioxide from tube C: Tube 1 (fermentation medium) color with phenol red pH (basic or acidic) yellow acidic Tube 2 (distilled H2O) red basic

6. Result of iodoform test from tube C: Tube number 1 2 3 Sample 2.5 ml distilled water 1.25 ml distilled water and 1.25% ethanol 2.5 ml medium from fermentation tube C Iodoform result (+/-) -

7. Drawing of few yeast cells under HPO.

Saccharomyces cerevisiae