step

Group1

1) enter cells counting data

Large Hemocytometer squars
counting
AVG
AVG*Dilution factot
Cells amount / ml
Dilution factor

*10^4
After dilution factor (*10^4)
*10^6

92
102
97
90
95.25
190.5
1.905

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Group2

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Group3

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2)
Method 1

Calculation for one well

Method 1 - Calculation for one well

enter wanted number of cells *10^6

0.5
100 cells = 0.0001 * 10^6

1,000 cells = 0.001 * 10^6

enter volume of culture medum in well (l)

2500

Method 2
Mix (A) + (B)

262.47

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10,000 cells = 0.01 * 10^6

2237.5

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Calculation for multiple wells

for each sample
Than dispense from the mix

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2500

l to take (Cells)
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100,000 cells = 0.1 * 10^6

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l to take (Medium)
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1,000,000 cells = 1 * 10^6

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to each well
Method 2 - Calculation for multiple wells

enter number of repeats (wells)

6

(A)
1837.27

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l to take to make a pool (Cells)

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(B)
15662.7

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l to take to make a pool (Medium)

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* Calculated with
1
well extra
* Cells amount and culture medium volume, calculated according to the data entered in Method1 fields.