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    Introduction Laboratory Techniques

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    am_ma271185
    This document discusses various laboratory techniques used in clinical chemistry. It describes spectral techniques like spectrophotometry, fluorescence, and atomic absorption spectroscopy. It also covers separative techniques such as precipitation, extraction, and chromatography. Immunochemical techniques involving precipitation, gel electrophoresis, and labeled immunoassays like ELISA are outlined. Finally, the document briefly introduces electrochemical techniques including potentiometry, voltammetry, conductometry, and coulometry.

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    Introduction Laboratory Techniques

    Uploaded by

    am_ma271185
    16 views10 pages
    This document discusses various laboratory techniques used in clinical chemistry. It describes spectral techniques like spectrophotometry, fluorescence, and atomic absorption spectroscopy. It also covers separative techniques such as precipitation, extraction, and chromatography. Immunochemical techniques involving precipitation, gel electrophoresis, and labeled immunoassays like ELISA are outlined. Finally, the document briefly introduces electrochemical techniques including potentiometry, voltammetry, conductometry, and coulometry.

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    This document discusses various laboratory techniques used in clinical chemistry. It describes spectral techniques like spectrophotometry, fluorescence, and atomic absorption spectroscopy. It also covers separative techniques such as precipitation, extraction, and chromatography. Immunochemical techniques involving precipitation, gel electrophoresis, and labeled immunoassays like ELISA are outlined. Finally, the document briefly introduces electrochemical techniques including potentiometry, voltammetry, conductometry, and coulometry.

    Copyright:

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    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    16 views10 pages

    Introduction Laboratory Techniques

    Uploaded by

    am_ma271185
    This document discusses various laboratory techniques used in clinical chemistry. It describes spectral techniques like spectrophotometry, fluorescence, and atomic absorption spectroscopy. It also covers separative techniques such as precipitation, extraction, and chromatography. Immunochemical techniques involving precipitation, gel electrophoresis, and labeled immunoassays like ELISA are outlined. Finally, the document briefly introduces electrochemical techniques including potentiometry, voltammetry, conductometry, and coulometry.

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    of 10

    LABORATORY TECHNIQUES

    Spectral techniques Separative techniques Immunochemical techniques Electrochemical techniques Osmometry


    Point-of-care testing

    SPECTRAL TECHNIQUES

    Spectrophotometry Luminescence techniques (fluorescence) Nephelometry & turbidimetry Reflectance spectroscopy Atomic absorption spectroscopy
    Atomic emission spectroscopy

    BASIC COMPONENTS OF A
    SPECTROPHOTOMETER

    BASIC COMPONENTS OF A
    SPECTROFLUORIMETER

    BASIC COMPONENTS OF A TURBIDIMETER

    BASIC COMPONENTS OF A NEPHELOMETER

    BASIC COMPONENTS OF A
    REFLECTOMETER

    SEPARATIVE TECHNIQUES

    Precipitation
    Ultrafiltration & dialysis Extraction Electrophoresis

    Chromatography

    IMMUNOCHEMICAL TECHNIQUES

    Precipitation Methods:

    In free solution (latex, haemagglutination, ) In gels: simple (e.g. SRID), assisted (e.g., Rocket IEP) Nephelometry and Turbidimetry

    Labeled IA: ELISA, RIA, FIA, CLIA,

    ELECTROCHEMICAL TECHNIQUES
    Ohm low: Current (A) = Potential (V)/Resistance

    Potentiometry Voltammetry including polarography and amperometry

    Conductometry

    Coulometry

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