The Journal of Nutrition Nutritional Immunology

Feeding a Diet Containing a Fructooligosaccharide Mix Can Enhance Salmonella Vaccine Efcacy in Mice1
Jalil Benyacoub,* Florence Rochat, Kim-Yen Saudan, Isabelle Rochat, Nicolas Antille, Christine Cherbut, Thierry von der Weid, Eduardo J. Schiffrin, and Stephanie Blum
Nestle Research Center, CH-1000, Lausanne 26, Switzerland

Abstract
Fructooligosaccharides (FOS) are considered prebiotics because of their ability to promote growth of specic benecial gut bacteria, such as bidobacteria. Some studies reported potential immune-modulating properties. The aim of this study was to investigate the effect of FOS:inulin mix on murine response to Salmonella vaccine and evaluate the relevance toward protection against Salmonella infection. Balb/c mice were fed a diet containing 5% FOS:inulin mix or a control diet 1 wk before oral immunization with a suboptimal dose of live attenuated Salmonella typhimurium vaccine. Four weeks after vaccination, mice were infected with LD100 of virulent S. typhimurium. Specic blood Salmonella immunoglobulin G and fecal immunoglobulin A signicantly increased in mice fed the diet containing prebiotics compared with control mice 4 wk postimmunization. Peritoneal macrophage phagocytic activity also signicantly increased in FOS:inulin-fed mice at 1 wk postimmunization compared with control mice. No detectable effects were observed on the percentage of lymphoid cell subsets in the spleen. However, production of cytokines, interferon-g , interleukin-12, and tumor necrosis factor a, was numerically increased in spleen cell cultures stimulated with mitogens from FOS:inulin-fed mice 1 and 4 wk postimmunization. Salmonella translocation to lymphoid organs was not affected by feeding FOS:inulin. However, the improved response to Salmonella vaccine was concomitant with an increase in the survival rate of FOS:inulin-fed mice upon challenge with virulent Salmonella. No detectable effects were observed on the composition or the metabolic activity of the microbiota. Overall, the data suggest that a diet supplemented with FOS:inulin mix stimulates mucosal immunity and seems to improve efcacy of an oral vaccine. J. Nutr. 138: 123129, 2008.

Downloaded from jn.nutrition.org by on January 17, 2009

Introduction
Prebiotics are dened as nondigestible food ingredients that benecially affect the host by selectively stimulating the growth and/or activity of 1 or a limited number of bacteria in the colon and thus improve host health (1). Fructooligosaccharides (FOS)2 selectively promote the growth of bidobacteria in the colon of humans and may decrease the count of potentially detrimental bacteria, thus being considered as prebiotics (13). Besides their bidogenic effect, short-chain FOS and long-chain FOS (such as inulin) have additional effects, including reducing colonic pH and increasing stool weight and stool bulking, justifying their classication as dietary bers (4). Furthermore, FOS and inulin are able to increase the bioavailability of calcium (5) and possibly inhibit hepatic lipogenesis (6).
1 Author disclosures: J. Benyacoub, F. Rochat, K.-Y. Saudan, I. Rochat, N. Antille, C. Cherbut, T. von der Weid, E. J. Schiffrin, and S. Blum, no conicts of interest. 2 Abbreviations used: CFU, colony-forming unit; PWM, pokeweed mitogen; FOS, fructooligosaccharide; IFNg, interferon-g ; Ig, immunoglobulin; IL-12, interleukin-12; MLN, mesenteric lymph node; NK, natural killer cells; PP, Peyers patches; PWM, pokeweed mitogen; Th1, T helper 1; TNFa, tumor necrosis factor-a. * To whom correspondence should be addressed. E-mail: jalil.benyacoub@rdls. nestle.com.

Immune-modulating effects of FOS were reported by several research groups. FOS increase leukocyte numbers in the gut mucosa and in the blood (7) and decrease pro-inammatory cytokines in human patients (8). Moreover, FOS were shown to indirectly enhance T cell functions, natural killer cells, and phagocytic activities through modulation of gastrointestinal tract lactic acid bacteria density, which protected mice from enteric and systemic pathogens and tumor inducers (9). The importance of Salmonella infections (particularly in developing countries) (10,11), the relatively low efcacy of the commercially available Salmonella typhy vaccine (12,13), and the increasing appearance of antibiotic-multi-resistant strains prompted the scientic community to design new vaccine strains (1416). The difculty was to obtain a strain highly attenuated, safe, and sufciently immunogenic to induce a signicant protective response (12,17). An alternative to improving the vaccine efcacy would be to use nutritional supplements that could stimulate the immune system as adjuvant. In that sense, several studies have shown that different nutritional interventions, including dietary nucleotides, probiotics, and prebiotics, could likely enhance immune responses to various vaccines in children and adults through a T helper 1 (Th1)-like-mediated adjuvant effect (7,1821).
123

0022-3166/08 $8.00 2008 American Society for Nutrition. Manuscript received 20 August 2007. Initial review completed 19 September 2007. Revision accepted 22 October 2007.

Salmonella has been suggested to preferentially cross the intestinal epithelial barrier through specialized M cells, found at the surface of the Peyers patches (PP), before disseminating to distant sites such as the liver and spleen (22,23). It is well documented that host resistance to Salmonella relies initially on the production of inammatory cytokines leading to the inltration of activated inammatory cells in the tissue, including interferon-g (IFNg ), tumor necrosis factor-a (TNFa), and interleukin-12 (IL-12). Thereafter, both T and B cell-mediated immune responses are elicited to control primary infection and protection against secondary challenge (24,25). In that context, secretory immunoglobulin (Ig) A produced in the intestine play a major role in the defense mechanism against these bacteria (15,26). The aim of this exploratory study was to investigate the capacity of a FOS:inulin mix to enhance murine immune response to Salmonella vaccine and evaluate its relevance for protection against Salmonella infection. Our working hypothesis is that a prebiotic combination such as the association of FOS and inulin could exert immuneenhancing functions that could be useful to protect the host against pathogens and/or could act as adjuvant for vaccines.

used as control for our prebiotic nutritional intervention studies (unpublished data). Bacterial strains and cultures. The 2 bacterial strains used were derived from the same genetic background, Salmonella typhimurium SL1344, wild-type strain (14), used as infectious strain and its derivative, Salmonella typhimurium SL1479 Daro A attenuated strain (ATCC39183), was used as vaccine. Bacteria were grown in Luria-Bertoni broth at 37C for 18 h and then concentrated in 1 mL of PBS. The number of viable cells was determined by agar plate counting. Identication of Salmonella was conducted using the API 20E test (BioMe rieux SA). Vaccination and infection protocols. The rst study aimed at evaluating the response to vaccine, 2 groups of mice were fed a control diet or a diet containing 5% FOS mix (n 20 per group). One week after starting the feeding, the 2 groups of mice were immunized by oral gavage with 5 3 107 colony-forming units (CFU) of attenuated S. typhimurium SL1479. This dose of Salmonella vaccine was established as conferring 50% protection against challenge with wild-type Salmonella strain in a previous study (unpublished data). The immune response was monitored during the 4 wk postimmunization that corresponds to the peak of antibody response (1416,20) (see Fig. 1 for trial design). Two separate subgroups of mice from each treatment group (n 5) were killed at 1 wk and 4 wk postvaccination to evaluate immune effects at the cellular level (peritoneal macrophages and splenocytes) and bacterial translocation vs. survival rate in lymphoid organs, respectively. A second study aimed at evaluating the protection against Salmonella infection, mice were randomized into 4 groups (n 20 per group). Group control were mice fed a control diet. Group control 1 vaccine were mice fed a control diet and vaccinated with a suboptimal dose of 5 3 107 CFU of attenuated Salmonella SL1479 prior to infection (as described above). Group FOS consisted of mice fed a diet containing FOS mix, and Group FOS 1 vaccine consisted of mice fed a diet containing FOS mix and vaccinated prior infection (Fig. 1). All groups of mice were infected 4 wk after vaccination or equivalent period (for unvaccinated mice) with 3 3 107 CFU per mouse of S. typhimurium SL1344 by oral gavage. This dose of virulent Salmonella was established as the LD100 in a previous study (unpublished data). The

Materials and Methods

Mice and diets. Conventional 6-wk-old female Balb/c mice (1820 g) were used in all experiments (Iffa-Credo). The mice were housed (5 mice per cage) under specic pathogen-free conditions at the Nestle research center animal facility. Mice were fed a commercial diet (Kliba 3434) supplemented with either 5% (wt:wt incorporated in the pellets) of a prebiotic mix (70% Raftilose P9530% Raftiline HP wt:wt in the mixed powder preparation, both from Orafti) or cellulose, hereafter called FOS mix or control, respectively. The macronutrient content as fed to mice was 17.6% protein, 4.3% fat, 4.3% crude ber, 51.5% carbohydrate, and 13.5 kJ/g metabolizable energy. The dose of prebiotics or cellulose corresponds to a daily intake of 810 mg/g of body weight. Such an amount of cellulose has never shown any effect on intestinal ecology or immunity in our previous animal studies, which is why it is commonly

Downloaded from jn.nutrition.org by on January 17, 2009

FIGURE 1 Trial design of control and treatment groups. Study 1: 2 groups were tested (mice fed a control diet or the same diet containing FOS-mix). Study 2: four groups were tested. Control, mice fed a control diet. Control + vaccine, mice fed a control diet and vaccinated. FOS, mice fed a diet containing FOS-mix. FOS + vaccine, mice fed a diet containing FOS-mix and vaccinated. 124 Benyacoub et al.

infection was followed daily during 3 wk, including weighing and macroscopic examinations. Criteria justifying euthanasia of mice included bristled coat, loss of weight (.20%), and loss of mobility. Mice were killed by cervical dislocation under anesthesia with isouorane. Assessment of antibody responses. Blood samples were collected from the tail vein of mice of study 1 just before vaccination and at 4 wk postimmunization. Samples were incubated overnight at 4C and centrifuged at 10,000 3 g; 15 min. Sera were collected and frozen at 220C until analysis for IgG. Fresh fecal samples were collected just before vaccination and at 4 wk postimmunization. Mice were individually isolated in a clean cage and fecal samples were collected right after emission. Samples were homogenized with extraction buffer (50 mmol/L EDTA, 100 mg/L soybean trypsin inhibitor in PBS; Sigma). After centrifugation at 10,000 3 g; 15 min, the supernatants were given doses for protein contents by BCA protein assay (Pierce) and stored at 220C until analysis for IgA. Total antibody levels (IgA in fecal extract or IgG in sera) were measured by ELISA as previously described (27). A monoclonal mouse IgA or mouse IgG was used as a standard and results were then expressed as the means 6 SEM of IgA or IgG in mg/L (the detection limits for both antibodies were 2 mg/L). The fecal IgA data were normalized to the protein content of each fecal extract. The amounts of specic anti-Salmonella IgA and IgG in feces were determined using the same ELISA except that the plates were coated with either 5 mg per well of Salmonella-LPS (Sigma) or 10 mg per well of Salmonella-agellin extracted as previously described (28,29). Briey, bacteria were grown overnight and agellin was isolated by acidic denaturation for 30 min at room temperature. Supernatant containing depolymerized agella was claried by centrifugation at 100,000 3 g; 1 h at 4C and equilibrated at pH 8.0. Flagellin was identied by SDSPAGE using the anti-agellin monoclonal antibody 15D8 (IGEN International). The data were expressed as mean OD at 450 nm 6 SEM. Assessment of phagocytic activity. Subgroups of control and FOS mix-fed mice from study 1 were killed 1 wk and 4 wk postimmunization (n 5 for each group at each time point). Peritoneal macrophages were collected in ice-cold sterile PBS. Macrophages were seeded into 48-well microtiter plates at a concentration of 5.105 cells per well and mixed with uorescent E. coli (E. coli:FITC) for 30 min (Phagotest-kit, Orpegen). Fluorescent macrophages, reecting the phagocytic activity, were analyzed by ow cytometry according to the manufacturers recommendations. Assessment of cell subsets, cell proliferation, and cytokine production. Spleen samples from subgroups of control and FOS mixfed mice from study 1, killed at 1 and 4 wk postimmunization (as described in the section above), were analyzed (n 5 for each group at each time point). Spleens were collected, homogenized through a cell strainer (70 mm) in 2 mL complete RPMI-1640 medium (Gibco), and centrifuged at 250 3 g; 5 min. The pellets were rapidly lysed with 1 mL of sterile distilled water and centrifuged at 250 3 g for 5 min. The cells pellets were then suspended in complete RPMI-1640 medium to obtain 109 cells/L. Cell subsets were quantied by 2-color ow cytometry according to the method previously described (27). Spleen cell proliferation assay was performed according to the method described by Humen et al. (30). Proliferation rates were evaluated on unstimulated cells or cells stimulated with the following mitogens (all purchased from Sigma): 2 mg/L concanavalin A (T cell stimulant), 1 mg/L pokeweed mitogen (PWM; B cell stimulant), or 2.5 mg/L LPS from S. typhimurium (monocyte and B cell stimulant). After incubation at 37C for 72 h, 0.15 mL of supernatant was collected for IL-12, INFg, and TNFa cytokine content analysis using ELISA kits purchased from R&D Systems according the manufacturers recommendations. As specied in the kit, the detection limits were 2.5 ng/L, 2 ng/L, and 5.1 ng/L, respectively. Quantication of Salmonella in lymphoid organs. As indicated above in the section Vaccination and infection protocols, subgroups of control and FOS mix-fed mice from study 1 were killed at 1 and 4 wk postimmunization, which correspond to the peak of Salmonella colonization and survival limit, respectively (31).

PP, mesenteric lymph nodes (MLN), spleen, and liver were asceptically collected, weighted, and homogenized in 2 mL of sterile PBS. The number of viable bacteria in each lymphoid organ was determined by plating 0.1 mL of processed tissue on Luria-Bertoni agar. Salmonella O antiserum (Becton Dickinson) was used for agglutination test to complete the identication of S. typhimurium. The minimal amount of Salmonella detectable is 20 CFU per total organ that corresponds to 1 colony per plate. Therefore, the limit of detection expressed in the results is log10 1.3. Analysis of microbiota. Fecal samples were collected from mice in study 1 24 h and 1 wk after immunization to analyze specically Salmonella content. In addition, fecal samples from the same mice were collected just before immunization (1 wk) and 4 wk after to evaluate microbiota composition. Freshly collected feces were homogenized in Ringer medium containing 10% glycerol, and enterobacteria, lactobacilli, bidobacteria, and enterococci or Salmonella, when specically evaluated, were immediately enumerated by plating on semiselective media following the method previously described (8). Statistical analysis. Statistical analyses were performed to evaluate differences between treatment groups of mice (mean values for each group represent the experimental unit for this nutritional intervention study). In study 1, differences among means (control vs. FOS) were evaluated for each variable within a sampling period using a 2-tailed Students t test. The antibody responses were further tested for changes over time using a 1-way ANOVA with repeated measures. Before each analysis, data were tested for normal distribution and homoscedasticity. In study 2, differences among survival rates at 3 wk postinfection (control vs. FOS vs. control 1 vaccine vs. FOS 1 vaccine) were evaluated using a chi-square test for independence. A condence level of 95% was applied for all tests. Values in the text are means 6 SEM.

Downloaded from jn.nutrition.org by on January 17, 2009

Results
Antibody responses (study 1). Neither total IgG levels in the sera nor IgA levels in the fecal contents were inuenced by FOS mix supplementation (data not shown). There were no differences in the specic anti-Salmonella antibody responses between the 2 groups at 1 wk postimmunization. The responses then signicantly increased in both groups at 4 wk postimmunization. However, this increased antiSalmonella response was much stronger in the FOS mix group compared with controls. Indeed, the specic-LPS-IgA levels in the fecal contents were greater in the FOS mix-fed group than in the control group (P 0.04) 4 wk after vaccination (Fig. 2A). The amount of specic agellin-IgG in the plasma was greater in the FOS mix-fed group than in the control group (P 0.01) 4 wk after vaccination (Fig. 2B). Stimulation of phagocytic activity (study 1). The phagocytic activity of peritoneal cells 1 wk postimmunization was higher in the FOS mix-fed group (52.5 6 0.7%) than in the control group (38.1 6 3.3%; P 0.02). After 4 wk, phagocytic activity in the FOS mix-fed group (40.5 6 0.4%) had returned to the level in the controls (39.3 6 0.6%). Spleen cell subsets, cell proliferation, and cytokine production (study 1). The percentage of cell subsets, including CD41, CD81, B2201, MHCII1, CD11b1, and CD11c1 cells, did not differ between FOS mix- and control mice. However, the mean uorescent intensity of MHCII1 cells was greater in FOS mix-fed mice (135 6 29.5) than in controls (89 6 21.4; P 0.03). Feeding the FOS mix did not signicantly inuence the splenocyte proliferation rates (data not shown). However, IL-12, IFNg, and TNFa cytokine concentrations in cell culture supernatants
Stimulation of vaccine response by fructooligosaccharides 125

FIGURE 2 Specific anti-LPS fecal IgA (A) and anti-flagellin serum IgG (B) measured 1 and 4 wk postvaccination in mice (study 1). Data are means OD 450 nm 6 SEM, n 10 duplicates. Fecal samples were diluted 1:20 and serum samples, 1:200 for sera. *Different from controls at that time, P , 0.05. #Different from the initial value, P , 0.05.

from FOS mix-fed mice tended to be higher than in controls when stimulated with mitogens at 1 and 4 wk postimmunization. IFNg and IL-12 levels were signicantly enhanced in mice fed FOS mix compared with controls at both time points when splenocytes were stimulated with LPS (Table 1). Recovery of Salmonella from lymphoid organs (study 1). The number of Salmonella recovered from PP, MLN, liver, and spleen at 1 wk after immunization did not differ between the 2 groups of mice with mean log10 CFU/g of 4 6 0.2 in PP, 2.7 6 0.1 in MLN, 2.3 6 0.1 in liver, and 1.5 6 0.3 in spleen. The numbers of salmonella decreased by more than 1 log in all organs in both groups at 4 wk postimmunization (data not shown). Microbiota analysis (study 1). There was no signicant effect of FOS mix on microbiota composition compared with controls. The fecal amounts of SCFA, including acetate, propionate, and butyrate, were not signicantly different between the 2 groups (data not shown). Salmonella were rapidly cleared after immunization with a similar ;2 log bacterial loss in both groups at 24 h postimmunization (log10 CFU/g of feces of 5.4 6 0.4 in control mice and 6 6 0.1 in FOS mix group) and barely detectable levels at 4 wk postimmunization (log10 CFU/g of feces of 3.3 6 0.1 in control mice and 2.8 6 0.1 in the FOS mix group). Protection against Salmonella infection (study 2). The survival rate in the control group was ;10% at 2 wk postinfection. This rate remained the same after 3 wk. FOS mix alone was not able to protect mice from Salmonella infection as demonstrated by the survival rate that was similar to the control group (i.e. 10% at the same time point). As expected, vaccination of mice fed a control diet with a suboptimal dose of
TABLE 1

attenuated Salmonella led to 40% protection (Fig. 3). This protection rate improved to 73% upon feeding FOS mix (P 0.04). In most cases, Salmonella infection leads to deaths between 7 and 15 d postinfection in nonprotected animals. During this period, the differences between the groups were in agreement with the ones described above at 3 wk postinfection.

Discussion
Downloaded from jn.nutrition.org by on January 17, 2009

This study shows that a FOS mix (FOS:inulin, 70:30 wt:wt in the mixed powder preparation) is able to improve the immune response to a Salmonella vaccine that contributes to increased vaccine efcacy. The idea of using foods as booster and/or carrier for human vaccine has been proposed in several studies [reviewed by Korban et al. (32)]. However, these approaches mainly consist of using genetically modied products, the use of which may be subject to regulatory issues in some countries. In this work, the data support the concept of using nongenetically modied food supplements containing a FOS:inulin mix to enhance vaccine efcacy. It was previously established that Salmonella strains mutated in genes involved in the aromatic biosynthetic pathway (aro mutants) were effective vaccines in several animal species (14,33,34). Immunization with Salmonella elicits a broad range of antigen-specic responses. In that respect, LPS and agellin have gained particular interest as potential protective antigens (3537). Therefore, both mucosal and systemic responses toward LPS and agellin were investigated in this study. We used a suboptimal dose of Salmonella vaccine to measure the potential effect of the dietary intervention. The low oral dose used in this study was still able to elicit detectable antibody titers, as observed in control mice. Specic anti-Salmonella antibody

Cytokine concentrations in cell culture supernatants of splenocytes from mice fed FOS mix or controls stimulated with different mitogens (Study 1)1
Wk 1 Wk 4 LPS ng/L 24 6 5 35 6 10 174 6 24 181 6 43 3132 6 228 4278 6 729 18 6 5 25 6 2 371 6 40 337 6 12 5608 6 476 6507 6 39 47 6 9 51 6 7 1091 6 189 1209 6 102 404 6 166 935 6 207* 42 6 2 64 6 2* 286 6 37 357 6 34 3919 6 389 5130 6 508 12 6 5 27 6 9* 569 6 63 722 6 61 4822 6 588 5785 6 491 25 6 6 26 6 9 843 6 117 857 6 114 40 6 19 210 6 55* ConA PWM LPS

Cytokines IL-12 Control FOS TNFa Control FOS IFNg Control FOS
1

ConA

PWM

Values are means 6 SEM, n 5 triplicates. *Different from controls, P , 0.05.

126

Benyacoub et al.

FIGURE 3 Survival rate of mice that were fed a diet containing FOS mix or a control diet and were vaccinated or not with an attenuated Salmonella prior to an infection with virulent Salmonella at 4 wk after immunization or equivalent period for unvaccinated mice (study 2). Data are expressed as the percentage of survival, n 20 for each group. *Different from control 1 vaccine at that time, P , 0.05. #Different from nonvaccinated mice, P , 0.05.

responses were signicantly enhanced in mice fed FOS mix compared with control mice, suggesting that a diet containing FOS mix might act as a mucosal adjuvant. One previous clinical trial has shown that the same FOS mix incorporated into cereals signicantly improved the response to measles vaccine in infants (20). Nevertheless, these data should be conrmed and the capacity of this prebiotic to trigger and boost systemic responses to vaccines needs further investigation. Uptake of Salmonella by phagocytic cells after the bacteria have crossed the epithelium represents a key feature of the innate response of the host to Salmonella infection (22,23). In this study, the percentage of peritoneal cell phagocytic activity was transiently but signicantly enhanced in the FOS mix-fed group compared with controls (P , 0.01 at 1 wk postimmunization). Along with this observation, the phagocytic activity as previously tested in vitro was signicantly enhanced after treatment of murine macrophage cell line J774 with 10 mg/L of FOS mix compared with nontreated cells (P 0.02), reecting the capacity of FOS mix per se to interact with immune cells (data not shown). The IL-12, IFNg , and TNFa cytokines are known to play a key role in the regulation of both innate and acquired immunity to Salmonella (24,38). The relative increase in these cytokine levels, mainly IL-12 and IFNg , seems to indicate an activation of T and B cells as well as macrophages. Moreover, the enhanced mean uorescent intensity of MHCII molecules upon feeding with FOS mix suggests a stimulation of antigen presentation in vivo. These results reect the capacity of FOS mix to promote a Th1-like adjuvant effect that might have supported the strong specic humoral responses observed in FOS mix-fed mice. The ability of S. typhimurium to translocate into lymphoid organs is known to be one of the major steps of its pathogenicity (39). In agreement with a previous publication (31), the DaroA mutant strain SL1479 was not impaired in its ability to colonize and survive in lymphoid organs. The number of Salmonella recovered from PP, MLN, liver, and spleen at 1 wk after immunization did not differ between the 2 groups of mice, suggesting that FOS mix per se did not inuence either the

translocation or the survival rates of Salmonella. These data are in contradiction with results previously published by Bruggencate et al. (40) and Bovee-Oudenhoven et al. (41). The authors suggest that feeding FOS (Raftilose P95) could increase Salmonella translocation in rats in a dose-dependent manner. Whether these discrepancies could be attributed to a difference in the doses of prebiotics or a difference in Salmonella strains used in the 2 studies could be questioned. We cannot exclude that differences in macronutrient and micronutrient contents of the diets used in the 2 studies could had differently impacted the prebiotic effect on the host. We did not observe any signs of immune suppression or other potential side effects in our study upon feeding with FOS mix. The dose used in this study corresponds well with the assumed dose of nondigestible carbohydrates consumed by humans, which is ;35% of daily dry food intake (42). The safety of FOS and the FOS:inulin mix is further supported by a few clinical trials in infants where no adverse effects have been observed. Importantly, no Salmonellosis has been reported so far upon feeding with prebiotics such as FOS and/or inulin in humans (4,20,43). We wanted to evaluate if feeding the FOS mix could either have a direct protective effect or confer indirect protection through an improved response to vaccine. Unvaccinated mice were not protected regardless of diet, suggesting that the FOS mix alone was not able to protect mice from Salmonella infection. These results do not corroborate previous data reported by Buddington et al. (9) showing that feeding Raftilose P95 or inulin protected mice from systemic Salmonella infection and prevented C. albicans translocation. This difference in outcomes might be explained by the differences in the doses of prebiotics used. However, differences in the diet composition and the routes of infections could also explain the results in the 2 studies. Our results showed that there was an improvement in the vaccine protection rate upon feeding with the FOS mix (from 40 to 73%). These results correlate well with the relative increase in the Th1 cytokine production and the high vaccine-specic immune response observed in FOS mix-fed mice at the time of challenge, i.e. 4 wk postimmunization, and are in agreement with the well-documented important role played by mucosal LPS-IgA and potentially systemic agellin-IgG in the prevention against Salmonella infection (3537,44,45). It is postulated that clinical benets from consumption of prebiotics are obtained through their effect on the colonic microbiota. Improved growth of lactic acid bacteria upon prebiotic feeding leads to a decrease in the pH that could contribute to the protection against pathogens (1,2,46). It is also assumed that modications in gut microbiota induced by prebiotics such as FOS may ultimately affect immune functions [reviewed by Schley and Field (7)]. We attempted to highlight the potential mechanism by which the FOS mix could inuence the immune system. No detectable effects were observed on microbiota composition or on SCFA fecal concentrations (data not shown). The prebiotic effect of the FOS:inulin mix, well studied in humans and other animals as indicated above (1,2,4,7,20,46), could not be featured in this model; however, because of limitations of the methods used in this study, we could not exclude it. Indeed, analysis of SCFA in the fecal content only partially reects the real colonic metabolism situation and the bacterial count on semiselective agar media presents some limitations in sensitivity. Thus, evaluation of the microbial ecology with other techniques such as uorescent in situ hybridization or denaturing gradient gel electrophoresis might help to better study the effect of FOS mix.
Stimulation of vaccine response by fructooligosaccharides 127

Downloaded from jn.nutrition.org by on January 17, 2009

The mechanism by which FOS impacts immunity still needs further investigation. Nevertheless, we can speculate on a possible direct interaction of the FOS mix with macrophages and/or dendritic cells underlying the gut mucosa either via a transfer through the epithelium or a direct sampling in the lumen by dendritic cells, that can penetrate the gut epithelial monolayer (47,48). However, in contrast to the dectin-1 receptor for b-glucans expressed by macrophages and dendritic cells (49), a receptor that might mediate FOS interaction with these cells, has not yet been identied. In conclusion, this study suggests that a diet enriched with a FOS:inulin mix is able to trigger and stimulate the gut mucosal immune system and supports the concept of using food supplements containing a FOS:inulin mix to enhance oral vaccine efcacy. Acknowledgments The authors thank M. Marchesini, J-L. Sanchez, M-A. Rochat, and P. Serrant for technical assistance.

19. Pickering LK, Granoff DM, Erickson JR, Masor ML, Cordle CT, Schaller JP, Winship TR, Paule CL, Hilty MD. Modulation of the immune system by human milk and infant formula containing nucleotides. Pediatrics. 1998;101:2429. 20. Haschke F, Firmansyah A, Meng M, Steenhout P, Carrie A-L. Functional food for infants and children. Monatsschr Kinderheilkd. 2001;149:S6670. 21. de Vrese M, Rautenberg P, Laue C, Koopmans M, Herremans T, Schrezenmeir J. Probiotic bacteria stimulate virus-specic neutralizing antibodies following a booster polio vaccination. Eur J Nutr. 2005;44: 40613. 22. Jones BD, Ghori N, Falkow S. Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M cells of the Peyers patches. J Exp Med. 1994;180:1523. 23. Clark MA, Jepson MA, Simmons NL, Hirst BH. Preferential interaction of Salmonella typhimurium with mouse Peyers patch M cells. Res Microbiol. 1994;145:54352. 24. Mastroeni P, Chabalgoity JA, Dunstan SJ, Maskell DJ, Dougan G. Salmonella: immune responses and vaccines. Vet J. 2001;161:13264. 25. Mittrucker HW, Raupach B, Kohler A, Kaufmann SH. Cutting edge: role of B lymphocytes in protective immunity against Salmonella typhimurium infection. J Immunol. 2000;164:164852. 26. Neutra MR, Pringault E, Kraehenbuhl JP. Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annu Rev Immunol. 1996;14:275300. 27. Benyacoub J, Perez PF, Rochat F, Saudan KY, Reuteler G, Antille N, Humen M, De Antoni GL, Cavadini C, et al. Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice. J Nutr. 2005;135:11716. 28. Steiner TS, Nataro JP, Poteet-Smith CE, Smith JA, Guerrant RL. Enteroaggregative Escherichia coli expresses a novel agellin that causes IL-8 release from intestinal epithelial cells. J Clin Invest. 2000;105:176977. 29. Gewirtz AT, Simon PO Jr, Schmitt CK, Taylor LJ, Hagedorn CH, OBrien AD, Neish AS, Madara JL. Salmonella typhimurium translocates agellin across intestinal epithelia, inducing a proinammatory response. J Clin Invest. 2001;107:99109. 30. Humen MA, De Antoni GL, Benyacoub J, Costas ME, Cardozo MI, Kozubsky L, Saudan KY, Boenzli-Bruand A, Blum S, et al. Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo. Infect Immun. 2005;73:12659. 31. Dunstan SJ, Simmons CP, Strugnell RA. Comparison of the abilities of different attenuated Salmonella typhimurium strains to elicit humoral immune responses against a heterologous antigen. Infect Immun. 1998; 66:73240. 32. Korban SS, Krasnyanski SF, Buetow DE. Foods as production and delivery vehicles for human vaccines. J Am Coll Nutr. 2002;21:S2127. 33. Fields PI, Swanson RV, Haidaris CG, Heffron F. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proc Natl Acad Sci USA. 1986;83:518993. 34. Hormaeche CE, Joysey HS, Desilva L, Izhar M, Stocker BA. Immunity conferred by Aro-Salmonella live vaccines. Microb Pathog. 1991;10:14958. 35. Brown A, Hormaeche CE. The antibody response to salmonellae in mice and humans studied by immunoblots and ELISA. Microb Pathog. 1989; 6:44554. 36. Harrison JA, Villarreal-Ramos B, Mastroeni P, Demarco DH, Hormaeche CE. Correlates of protection induced by live Aro-Salmonella typhimurium vaccines in the murine typhoid model. Immunology. 1997; 90:61825. 37. McSorley SJ, Cookson BT, Jenkins MK. Characterization of CD41 T cell responses during natural infection with Salmonella typhimurium. J Immunol. 2000;164:98693. 38. Mastroeni P. Immunity to systemic Salmonella infections. Curr Mol Med. 2002;2:393406. 39. Gautreaux MD, Deitch EA, Berg RD. Bacterial translocation from the gastrointestinal tract to various segments of the mesenteric lymph node complex. Infect Immun. 1994;62:21324. 40. ten Bruggencate SJ, Bovee-Oudenhoven IM, Lettink-Wissink ML, van der MR. Dietary fructo-oligosaccharides dose-dependently increase translocation of salmonella in rats. J Nutr. 2003;133:23138. 41. Bovee-Oudenhoven IM, ten Bruggencate SJ, Lettink-Wissink ML, van der MR. Dietary fructo-oligosaccharides and lactulose inhibit intestinal colonisation but stimulate translocation of salmonella in rats. Gut. 2003;52:15728.

Literature Cited
1. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiata: introducing the concept of prebiotics. J Nutr. 1995;125: 140112. Gibson GR. Dietary modulation of the human gut microora using prebiotics. Br J Nutr. 1998;80:S20912. Gibson GR, Beatty ER, Wang X, Cummings JH. Selective stimulation of bidobacteria in the human colon by oligofructose and inulin. Gastroenterology. 1995;108:97582. Roberfroid MB. Health benets of non-digestible oligosaccharides. Adv Exp Med Biol. 1997;427:2119. Scholz-Ahrens KE, Schaafsma G, van den Heuvel EGHM, Schrezenmeir J. Effects of prebiotics on mineral metabolism. Am J Clin Nutr. 2001;73: S45964. Delzenne NM, Kok N. Effects of fructans-type prebiotics on lipid metabolism. Am J Clin Nutr. 2001;73:S4568. Schley PD, Field CJ. The immune-enhancing effects of dietary bres and prebiotics. Br J Nutr. 2002;87:S22130. Guigoz Y, Rochat F, Perruisseau-Carrier G, Rochat I, Schiffrin EJ. Effects of oligosaccharide on the faecal ora and non-specic immune system in elderly people. Nutr Res. 2002;22:1325. Buddington KK, Donahoo JB, Buddington RK. Dietary oligofructose and inulin protect mice from enteric and systemic pathogens and tumor inducers. J Nutr. 2002;132:4727. Edelman R, Levine MM. Summary of an international workshop on typhoid fever. Rev Infect Dis. 1986;8:32949. Mahle WT, Levine MM. Salmonella typhi infection in children younger than ve years of age. Pediatr Infect Dis J. 1993;12:62731. Kelly SM, Bosecker BA, Curtiss R III. Characterization and protective properties of attenuated mutants of Salmonella choleraesuis. Infect Immun. 1992;60:488190. Levine MM, Ferreccio C, Abrego P, Martin OS, Ortiz E, Cryz S. Duration of efcacy of Ty21a, attenuated Salmonella typhi live oral vaccine. Vaccine. 1999;17 Suppl 2:S227. Hoiseth SK, Stocker BA. Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature. 1981;291:2389. Levine MM, Galen J, Barry E, Noriega F, Chateld S, Sztein M, Dougan G, Tacket C. Attenuated Salmonella as live oral vaccines against typhoid fever and as live vectors. J Biotechnol. 1996;44:1936. Chateld S, Roberts M, Londono P, Cropley I, Douce G, Dougan G. The development of oral vaccines based on live attenuated Salmonella strains. FEMS Immunol Med Microbiol. 1993;7:17. Bumann D, Hueck C, Aebischer T, Meyer TF. Recombinant live Salmonella spp. for human vaccination against heterologous pathogens. FEMS Immunol Med Microbiol. 2000;27:35764. Link-Amster H, Rochat F, Saudan KY, Mignot O, Aeschlimann JM. Modulation of a specic humoral immune response and changes in intestinal ora mediated through fermented milk intake. FEMS Immunol Med Microbiol. 1994;10:5563.

Downloaded from jn.nutrition.org by on January 17, 2009

2. 3.

4. 5.

6. 7. 8.

9.

10. 11. 12.

13.

14. 15.

16.

17.

18.

128

Benyacoub et al.

42. Coussement PA. Inulin and oligofructose: safe intakes and legal status. J Nutr. 1999;129:S14127. 43. Guesry PR, Bodanski H, Tomsit E, Aeschliman J-M. Effect of 3 doses of fructo-oligosaccharides in infants. J Pediatr Gastroenterol Nutr. 2000; 31:S252. 44. Kraehenbuhl JP, Neutra MR. Molecular and cellular basis of immune protection of mucosal surfaces. Physiol Rev. 1992;72:85379. 45. Michetti P, Mahan MJ, Slauch JM, Mekalanos JJ, Neutra MR. Monoclonal secretory immunoglobulin A protects mice against oral challenge with the invasive pathogen Salmonella typhimurium. Infect Immun. 1992;60:178692.

46. Cummings JH, Macfarlane GT. Gastrointestinal effects of prebiotics. Br J Nutr. 2002;87 Suppl 2:S14551. 47. Brandtzaeg P, Baekkevold ES, Morton HC. From B to A the mucosal way. Nat Immunol. 2001;2:10934. 48. Rescigno M, Urbano M, Valzasina B, Francolini M, Rotta G, Bonasio R, Granucci F, Kraehenbuhl JP, Ricciardi-Castagnoli P. Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria. Nat Immunol. 2001;2: 3617. 49. Brown GD, Gordon S. Immune recognition. A new receptor for betaglucans. Nature. 2001;413:367.

Downloaded from jn.nutrition.org by on January 17, 2009

Stimulation of vaccine response by fructooligosaccharides

129