International Journal of Emerging Trends & Technology in Computer Science (IJETTCS)

Web Site: www.ijettcs.org Email: editor@ijettcs.org, editorijettcs@gmail.com Volume 2, Issue 2, March April 2013 ISSN 2278-6856

Synthesis and antimicrobial activity of Cr(III) and Co(II) complex of substituted benzoinhydrazones
P.M.Dahikar1 , R.M.Kedar2 , S.D.Patil3
Shri .R.R.Lahoti Science college, Morshi Shri shivaji science college, Amravati, Dist.Amravati

Abstract-Novel metal benzoinhydrazone complexes have

been synthesized from substituted benzoinhydrazone. They were characterized by complexes were screened for antimicrobial activity at a concentration of 1000gm/ml. Which was serially diluted to determine their MIC value of benzoinhydrazone-Cr(III)

Keywords - Antimicrobial activity, Cr(III) complexes, 4dimethylamino benzoinhydrazone. 2hydroxybenzoinhydrazone

analysis.In order to synthesize the complexes,the equimolar mixture of each of the ligand (0.01m) and metal salts were refluxed on a water both for 6-8 hours in presence of sodium acetate in ethanol / methanol the reaction mixture was kept overnight. The product formed were isolated washed several times with cold water ethanol mixture. The characterization of syntherized complex was made with elemental analysis, IR and UVVIS Spectra.

1. INTRODUCTION
Benzoinhydrazone are well known for their biological activity coordination compounds containing ONS as donor atoms are reported to possess antimicrobial activity. Several substituted benzophenone nicotinyl and isonicotinyl, hydrazone have been prepared and tested for their activities on aspergillus niger and by Shrivastava2 S.aureus was carried out by Shrivastava2 .Scherbakov3 reported the complex formation of Benzoin hydrazone with copper (II) and Nicked (II), Baligar 4 reported carried out synthesized new menoudeating hydrazone in Co(II), Ni(II), Zn(II) complexes and characterized them in detailed. Synthesis, spectral and biological studies of Co(II), Ni(II), Zn(II), Cu(II) and Cd(II) complex with Benzil salicyladehyde acyldihyxazone were carried out by Singh5. Similar Studies of N-heteroaromatic hydrazones and their complexes with pd(II) and Cd(II) were carried out by filipovic6.The spectroscopic characterization and biological activities of salicyladehyde thiazolyl hydrozone liganda and their metal. Complexes were carried out by cukurovadi7. prasad8 . The Novel Synthesis of Ni (II) complexes with new ligand derived from hydrazine of isonizid and studied their magnets- spectral, electrochemical, thermal and antimicrobial properties reported by prasad8.

3. PHYSICAL MEASUREMENT
C, H and N were analysed on a carlo-Erba-1106 elemental analyser Magnetic susceptibility was measured at room temperature on a Gouy balance using Hg [Co(SCN)4] as calibrant 1 HNMR spectra were recorded on Brucker AC 300F spectrometer with TMS as internal standard using CDCl3 and DMSO-D6 as a solvent. IR spectra (KBr) were recorded on perkin Elmer spectrometer in range 4000-400 cm1 in KBr pellets. All chemicals used were of AR-grade.

4. RESULT AND DISCUSSION

IR spectra of ligand 4-DMABH show band at 3423cm-1 (O-H).In complexes 4-DMABH-Cr(III)complexes show bands at decrease to 3355cm-1 indicating through hydrazone oxygen. However,1597cm-1(C=N) significantly decreases to 1543cm-1showing linkage through imino nitrogen.IR spectra of ligand 4 4'-DMBH show band at 3388cm- 1 (O-H).In complexes 4 4'-DMBH-Cr(III) which decrease to 3357cm-1 indicating through hydrogen oxygen.However 1634(C=N)significantly decrease to 1581cm-1 showing linkage through imino nitrogen.IR spectra of ligand 2-HBH show band at 3412cm-1.In complexes 2-HBH-Cr(III)which decrease to 3327cm-1 indicating through hydrogen oxygen.However1607(C=N) significantly decrease to 1555cm-1 showing linkage through imino nitrogen. Table 1:IR spectral data of ligands and complexes

2. EXPERIMENTAL SECTION
The benzoinhydrazone were prepared by refluxing substituted benzoines with hydrate in presence of alkaline medium for 3-4 hours, the reaction mixture were kept overnight. The solid products formed were isolated and washed several times with water alcohol mixture the purity was checked by TLC paper. Their structural details were confirmed on the basis of elemental and spectral Volume 2, Issue 2 March April 2013

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International Journal of Emerging Trends & Technology in Computer Science (IJETTCS)

Web Site: www.ijettcs.org Email: editor@ijettcs.org, editorijettcs@gmail.com Volume 2, Issue 2, March April 2013 ISSN 2278-6856
Co(II) 4,4'DMBHCo(II) 2-HBHCo(II)

Ligands and its Complexes 4-DMABH 4,4'-DMBH 2-HBH 4-DMABH-Cr (III) 4,4'- DMBHCr (III) 2-HBH(III) Cr

(O-H)

(>C=N)

(C-O)

(MO) 4872

(MN) 559

4.72

13604,19018, 23023 13605,19230, 222222

149 2 148 5

715

0.7 3 0.6 4

27

3423 3388 3412 3355

1597 1634 1607 1543

1388 1362 1417 1373

4.80

657

36

3357

1581

1243

465

580

3327

1555

1367

474

593

4-DMABH-Co (II) 4,4'-DMBHCo (II) 2-HBH-Co (II)

3395

1583

1384

515

579

3351

1624

1308

466

578

3353

1570.8

1400.3

507.3

587

On the basis of elemental analysis the complexes were assigned to possess the composition as shown in Table 2
Complexes Colou r Deco mposi tion Temp 286o C 288o C 289o C 279o C 283o C 284o C Elemental Analysis Found / (Calculated) % C 64.27 (65.3 0) 60.76 (61.5 3) 63.01 (62.9 2) 63.61 (64.5 4) 59.75 (60.8 6) 61.35 (62.1 1) H 5.08 (6.12) 4.89 (5.76) 3.95 (4.86) 5.00 (6.05) 4.84 (5.70) 3.91 (4.80) N 14.28 (14.2 8) 8.97 (8.95) 10.48 (10.4 8) 14.01 (14.0 2) 8.87 (8.87) 10.34 (10.3 5) M 7.93 (8.8 4) 7.47 (8.3 3) 8.85 (9.7 3) 8.97 (9.9 0) 8.45 (9.3 4) 9.97 (10. 89)

The electronic spectrum of 4-DMABH-Cr(III) Complex exhibits three transitions in the range 13572,18903,23400 cm-1. These spectral bands may be assigned to the following transitions 4T1g(F) 4T2g (F), 4T1g (F) 4A2g (F), 4T1g(F) 4T1g(P) Characteristic to an octahedral geometry9. The magnetic moment of 3.99 BM for Cr (III) complex is consistent with octahedral geometry around Cental metal ion10. 4-DMABH - Co(II) complex exhibit absorption bands at 13670, 19194, 21432 cm-1 which may be consign to 4A2g 4T1g ,4A2g 4T1 g(F) and 4A2g 4T1g (F) transition respectively11-12 suggesting on octahedral geometry around a cobat (II) ion in the complexes under Study, Further more, the magnetic moment measurement recorded at room temperature lies at 5.1 B.M. This values is indicates of an octahedral geometry of there complex1314 .The calculated values of ligand field splitting energy(10Dq),Racah interelectronic repulsion parameter() and % covaleancy. As shown above table. Antimicrobial Activity of Complexes :The compounds were assayed for their antimicrobial activities.15 Against four test organisms. E, coli, S. aureus, P. aeruginosa, B. Subtilis At a concentration of 1000 gm / ml by agar well technique16 Further their MIC value against these organisms were determined by serial dilution method15 . Using DMF as a solvent, the resuts obtained are given in the following table. Table 4: MIC values in gm / ml of compounds.
Complexes 4-DMABHCr(III) 4,4'-DMBHCr(III) 2-HBHCr(III) 4-DMABHCo(II) 4,4'-DMBHCo(II) 2-HBHCo(II) E,coli 250 125 125 125 125 125 S.aureus 250 125 125 63 250 63 P. aeruginosa 250 125 63 125 125 63 B.Subtilis 125 250 125 125 125 125

4-DMABHCr(III) 4-4'DMBHCr(III) 2-HBHCr(III) 4-DMABHCo(II) 4-4'-DMBHCo(II) 2-HBHCo(II)

Brow n Green ish Brow n Green

Brow n Brow n

Table 3 :- Magnetic Moment and Electronic Spectral Data (Cm-1) of the metal complexes
Complexes eff. (B.M. ) 4.18 max(Cm-1) Dq B1 B % Covale ncy 29

4DMABHCr(III) 4,4'DMBHCr(III) 2-HBHCr(III) 4DMABH-

13572,18903, 23400 13490,19605, 22272 13886,19570, 22484 13670,19194, 21432,

135 7 147 4 151 8 148 4

655

0.7 1 0.7 2 0.7 5 0.6 1

4.19

669

28

On the basis of MIC values,2-HBH-Co(II) is found to be most effective antimicrobial agent follow by 2-HBH Cr(III) and 4-DMBH-Co(II).The enhance antimicrobial activity in case of the compounds 2-HBH-Co(II) may be attributed to the presence of hydroxyl group. REFERENCES [1] Pettering H.G. and Crime J.A., Cancer Res, 27, 1278, 1967. Page 238

4.23 4.70

693 595

25 39

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International Journal of Emerging Trends & Technology in Computer Science (IJETTCS)

Web Site: www.ijettcs.org Email: editor@ijettcs.org, editorijettcs@gmail.com Volume 2, Issue 2, March April 2013 ISSN 2278-6856
[2] (Miss) K. Shrivastava and J. K. Mehrota, J. I nd. Chem. Soc., 38(12), 1015-1017, 1961. [3] I. N. Shcherbakov, I.D. PopoV, S.I. Levchenkov, A.N. Morogov, A.V. Kogan, and A.D Vikrishchuk. [4] R. S. Baligar and V. K. Revankar, J. Serb. Chem Soc. 71 (12), 1301-1310, 2006 [5] V. P. Singh ; P.Gupta ; N. Lal. Russian J. Coordination Chem ; 34 (4), 270-277, 2008. [6] N. Filipovic ; T. Todorovic ; R. Markovic ; A. Marinkovic ; S. Tubegdzic ; D. Godevac ; K. Andelkovic, Transition Mt. Chem ; 243-245, 2010. [7] A cukurovali and I. Yilimaz, Transition amet-Chom, 31,207-213, 2006. [8] S. Prasad and R. K. Agrawal, Research Letters in Inogranic Chem, 10,1-4, 2008. [9] S. Chandra and Anil Kumar, J. Saudi Chem. Soc ; Vol. 11, NO. 2 ; pp 299-306, 2007 [10] S. Chandra, Monika Tyagi ; J. Indian Chem. Soc ; Vol. 85, pp ; 42-47, Jan 2008 [11] AA. El-Asmy ; M.E. Khalifa ; M.M. Hassanian ; Ind. Of chem. ; 43 (A), 92-97, 2004 [12] M.A. Monshi, J. Ind. Chem-Soc, 75, 158-159, 1998 [13] K Nakamoto, Infrareal and Raman Spectra of Innorganic and coordination compounds willeInterscience, New York, 3rd Ed. 171 ,1978 [14] N. Nora ; Al Shaalan ; molecules ; 12, 1080-109, 2007. [15] C. D. Donald and A. R. William, In Assay methods of antibiotics. A Lab Manual medical Encyclopedia Inc. 1955 [16] Egorow N. S. Antibiotics, A. Scientific approach mir publishers,Moscow,136, 170-171 ,1985

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