Professional Documents
Culture Documents
ProntoEvolution Manual de Servicio
ProntoEvolution Manual de Servicio
TABLE OF CONTENTS
MAINTENANCE SCHEDULE.........................................................................................2 SECTION 1. INTRODUCTION.......................................................................................3
1.1 GENERAL DESCRIPTION.................................................................................................3 1.2 TECNICAL SPECIFICATIONS.......................................................................................5 1.3 INSTALLATION...................................................................................................................6 1.4 STARTING THE ANALYZER and the MAIN MENU......................................................7
2.12 IMMUNOCHEMISTRY....................................................................................................32
SECTION 3.
3.1
PROGRAMMING CHEMISTRIES...............................................................................36
3.2 ASSIGNING AND MODIFYING METHODS................................................................37 3.3 PRINT METHODS............................................................................................................39 3.4 REARRANGING SEQUENCE OF METHODS.............................................................39 3.5 3.6 3.7 3.9 3.10 CONFIGURATING PROFILES.....................................................................................40 FORMATTING REPORT HEADER.............................................................................40 CONTROL SERA.............................................................................................................40 SETTING DATE AND TIME.........................................................................................42 DISK COPIES................................................................................................................42
SECTION 4.
Page 1 of
67
SECTION 5.
5.1 DISASSEMBLING THE CABINET.................................................................................55 5.2 REPLACING THE SYRINGE...........................................................................................56 5.3 REPLACING THE LAMP/CALIBRATION...................................................................57 5.4 REPLACING A FLOW-CELL..........................................................................................58 5.5 REPLACING TUBING OF PERISTALTIC PUMP........................................................59
APPENDIX 6-A................................................................................................................60 APPENDIX 6-A................................................................................................................61 APPENDIX 6-A................................................................................................................62 APPENDIX 6-B................................................................................................................64 APPENDIX 6-B................................................................................................................65 APPENDIX 6-B................................................................................................................65 APPENDIX 6-C................................................................................................................66
MAINTENANCE SCHEDULE
PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 2 of
67
QUARTERLY MAINTENANCE
YEARLY MAINTENANCE
Page 3 of
67
Page 4 of
67
Reagent compartment : 15 reagent containers 45ml each - Electronic cooling system Reaction compartment: 180 positions:10 sectors of 18 wells Photometer : 6 interference filters + 1 option - range 340 to 620 nm resolution 0.0001 Abs. - Quartz-Tungsten Lamp, 10 Watt 50l flow cell temperature control by peltier elements:37C 0.1 : variable from 50-900 ul : variable from 2-100 ul : External DOS-compatible 386/486 option : 14", color SVGA : alfanumeric Std US : std High Speed Graphic printer on-board, 40 characters optional External printer. : RS 232 C : Printer Centronics Compatible : alphanumeric, patient by patient, or test by test. Date, patient ID and patient name. : 68 x 55 x 41 cm (W x D x H) : 40 Kg. :220 V - 50 Hz optional : 110 V - 60 Hz :400 Watt
Reagent volume Sample volume Processing Video display Keyboard Printer Serial port Parallel port Reporting Dimensions Weight Power requirements Power consumption
Page 5 of
67
! IMPORTANT
If the instrument must be powered with 110 V , please refer to APPENDIX D to perform the power voltage change. If Voltage is not changed, malfunctioning or more severe damages can occurr. For the power,use a "clean" line, with not too big variations in voltage, and provided with a ground. Avoid to place the instrument into direct sunlight. In case your PRONTO Evolution has been purchased with the computer, the custom interface board has been already installed into the computer, in this case you need only to connect the flat cable from the card mounted into computer, to the rear connector of PRONTO Ev. analyzer. NOTE - Its important to connect the ground wires of the flat cable to the chassis of the instrument and to the chassis of the computer. In case you have purchased PRONTO Evolution to be installed with your own computer, follow these instructions: 1. With PRONTO Ev. is supplied a Custom Interface Board to be installed into a slot of your computer's mother board.This PCB is electrostatic sensitive, so handle it with care and take it off its plastic bag just immediatly before installation. 2. Disassemble the case of your computer after having disconnected it from AC line. 3. Insert the PCB PRONTO Ev. interface Board into a slot of your computer's mother board. 4. It's not necessary to check the IRQ of other boards present into your computer and beware their IRQ numbers don't match IRQ of PRONTO interface board, as this PCB doesn't work in Interrupt. 5. Assemble cover of the computer and complete connections with PRONTO Ev. and other peripherals equipments.
Page 6 of
67
! IMPORTANT
The software will detect automatically the presence of an hard disk and will ask you to install it onto the hard disk drive, in order to start it automatically at the boot. Once the software has been installed onto the Hard Disk and the computer has been rebooted or once the computer has been switched on with the floppy disk inserted into the drive, the program is loaded automatically, then the following message will be prompted on the screen: SWITCH ON THE INSTRUMENT Now you can switch on PRONTO Evolution by the main switch located on the rear of the instrument, after a while the MAIN MENU appears, with the 3 main selections: PATIENTS ANALYZER UTILITIES HELP 22-09-1997 12:10:54
Rev. 1.7
The option PATIENTS offers access to all patient data and results. The option ANALYZER offers access to all functions of the analyzer, from routine work and methodology till maintenance. The option UTILITIES offers access to all other functions.The option HELP it's just a brief comment to keyboard functions. With the cursor keys and it's possible to step from one selection to the other or, in case no menu' is selected, just type the letter that appears highlighted (i.e. ANALYZER).
Once the menu name is selected with the arrow keys, hit ENTER and the sub-menu of the selected option is being displayed: PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 7 of
67
1-ENTER PATIENTS E REQUESTS 2-PRINT PATIENT REPORTS 3-PRINT RESULTS PER TEST 4-LIST PATIENTS 5-WORKLISTS 6-ENTER MANUAL RESULTS 7-RECEIVE PATIENTS 8-TRANSMIT RESULTS
PATIENTS
ANALYZER
UTILITIES
HELP
22-09-1997
12:10:54
1-START ANALYZER 2-CALIBRATION 3-METHODS PROGRAMMING 4-MAINTENANCE 5-MECHANICAL TESTS 6-QUALITY CONTROL 7-PREDILUTION 8-REPEAT TESTS 9-IMMUNOCHEMISTRY
PATIENTS
ANALYZER
UTILITIES
HELP
22-09-1997
12:10:54
1-SET DATE 2-SET TIME 3-COPY DISK 4-SET REPORT HEADER 5-SET PROFILES 6-SET ASSAY VALUES CONTROLSERA 7-TEST-CODES for ON-LINE 8-COPY PREVIOUS PATIENTS
Each of the sub-options can be selected by going there with the cursor keys and hitting ENTER, or by just typing the corresponding number.
Page 8 of
67
And so on till reaching the patient number 200. Of course in this case the second or third tray are virtual as physically we do not change the tray. In case we select the sample tray = 58 the relation becomes the following : Pat.No. Position No. 1 1 . . 58 58 59 1 2nd tray . . 116 58 117 1 3rd tray . . Of course in this case we use only serum cups and not primary tubes too.
The patient number is set to 1, when the active patient record is being cleared and increments automatically every time a new patient is entered, either manually or on-line. Furthermore every patient has a field for a Patient ID of 8 characters (alpha- numeric). This patient ID can be the same as the patient number, or in case of a bigger labcomputer, it can correspond to the patient number in the labcomputer. This is PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 9 of
67
Patient number ?1
TEST:
F) GPT P) UA Z) PHL j) C3
G) GLU Q) CA a) BIL k) C4
J)FAC T) CL d) IGA
F1=Next F2=Stat
F3=C1
F4=C2
F5=Patient
F9=Preceeding
Entering Patient no.: XXX Here the current patient no. is displayed.With F1 and F9 you can page through the patients already entered. Patient ID. : Up to 8 alphanumeric characters corresponding to a hospital or laboratory ID.
Patient Name : Up to 30 alphanumeric characters for pat. demographics. Both fields will appear on the patient report. TEST : You can select it with: - a Test-ID letter as displayed in the block below - a number of a profile as configurated in the Profiles ( Sec. 3.5 ) - a test can be deleted by typing it again.
TEST-BLOCK
Here the test-ID of all configurated chemistries are being displayed. Each test selected is displayed in inverse-mode. An alternative mode to select tests, is to go into the test to be selected by means of the cursor keys and hitting the ENTER key. By hitting the ENTER-key again a test can be defeated.
Once a patient is ready, hit F1 to go to the next number.Hitting F9 you go to previous patient.When the last patient has been entered, hit ESC to return to the main menu. PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 11 of
67
When we select this option, we have the following mask on the screen: PATIENTS ANALYZER UTILITIES HELP 22-09-1997 12:10:54
DELETE PATIENTS
Page 12 of 67
At this moment we can select the two options displayed; if we select RECEIVING PATIENTS, we will add these patients to the patients which are already present in the instrument, if we select DELETE PATIENTS, the effect will be exactly the same described in the section ENTERING PATIENTS, by resetting the progressive number to 1 and deleting all the patients present. Once we select RECEIVE PATIENTS, the system will contact the host computer by sending an enquire for connection, if there is contact with the host computer the PRONTO Evolution displays: >> COMPUTER ON LINE << When patients are received they are being displayed.When nothing appears, it means that the computer is either not ready or there are no new patients to be loaded. You can escape from this program any time with the key ESC. NOTE - The patients received from a lab computer can be resent to the lab computer, after the tests have been completed, with the option TRANSMIT RESULTS, but if we want to send to the computer also some patients which have not been downloaded from the lab computer, but have been entered directly into the PRONTO, we must use the option UNIDIRECTIONAL, in order to send to the lab computer also the patients entered directly on the PRONTO (i.e. URGENCIES).
For any further information about this operations, refer to your lab data handling software operations manual.For any data implementation with an existing lab software refer to appendix 6-B of this manual for transmission and data protocol description. When all the tests have been completed for all the patients, then its possible to send back the results to the host computer by the selection of the TRANSMIT RESULTS function. The screen will display : PATIENTS ANALYZER UTILITIES HELP 22-09-1997 12:10:54
Page 13 of
67
By selecting the option PRINT, all patients' list is printed by the internal printer. PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 14 of
67
By selecting the patient number using the arrow key, selected patient will be lighted up and on the right column will be displayed all results and tests related to that patient.To escape from this menu press the ESC key.
By the choose of ALL TESTS, a complete worklist for all tests will be printed, by selecting SELECT TEST option, will appear this screen:
PATIENTS ANALYZER UTILITIES HELP WORKLIST EXISTING TESTS: 12-06-1995 Esc=Exit
F) GPT P) UA Z) PHL j) C3
G) GLU Q) CA a) BIL k) C4
Select the tests you need worklist for and press ESC key to start printing. PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 15 of
67
WORKLISTS on date : 22-09-1997 TEST : BUN - UREA R 1414 NUM. PAT. ID. PAT. TOT. TESTS : 6 *********************************************** 3) 01002 ..................... 5) 01050 ..................... 6) 01051 ..................... 8) 01053 ..................... 9) 01054 ..................... 10) 01055 .....................
2.5 CALIBRATION
As PRONTO Evolution is a random access analyzer, it's not necessary to enter all patients before performing calibration, but it's possible to calibrate up to 10 tests before entering any patient. Furthermore it's possible to perform calibration against standard or use the factor obtained with a previous calibration. To carry out all calibrations required select the option CALIBRATION from the ANALYZER-menu. The following screen is displayed:
PATIENTS ANALYZER UTILITIES HELP 12-06-1997 Esc=Exit
ANALYZER OPERATING CALIBRATE ? *(CALIBRATED) A) TP B) BUN C) CHO D) GOT E) TRI F) GPT G) GLU H) GGT I) CRE 67 J)FAC
Page 16 of
F2 = View
F3 = Print * ( Calibrated)
In the lower block the tests that can be calibrated are being displayed, together with the number of samples for each. To select tests to be calibrated it's possible to proceed in different ways: 1- Typing letter or capital letter beside test to be calibrated, remember that a small letter and a capital letter are not indicating the same test. 2- Selecting profile number to calibrate all test grouped in that number 3- Moving with arrow keys and typing Enter key to select tests When tests to be calibrated are selected, press F1 to continue and execute calibration. Beside test codes, in square parenthesis are indicated the number of samples per test. NOTE* If calibration of a test has never been performed, it will not be possible to run that test, if no test is calibrated,the instrument will not start operations, even if patients and requests are entered. By this is necessary to run at least a first time a calibration, then the calibration frequency will be up to the end user only. The instrument keeps in memory the calibration factor and indicates it with an * beside the test.
Another point to remark is that to operate with PRONTO Evolution, you don't need necessarily to enter patients, as working in true random mode, it's possible to enter patients while instrument is running. Here there are three choices, F1 to continue the calibration, F2 to view and F3 to print the previous calibration datas ( i.e. the calibration factors of yesterday). NOTE* that all test marked with an asterisk ( * ) are already calibrated ! After having pressed F1 Key, will be prompted the following screen:
PATIENTS ANALYZER UTILITIES HELP 12-06-1997 Esc=Exit
EXECUTE TEST
TEST GLU GOT CHO BUN STD POS. 1 1 1 REAG.POS. 15 2 6 12 REAG.VOL.ml REAG.VOL.ml (Exist.) 42 38 32 18 00 00 00 00 STD 290 150 46 FACTOR 323.2 1768 612 1210 CONTROLS C1 C2 C1 C1C2 C1
F1=CONTINUE
F9=CALIBRATION Page 17 of
67
Pressing F9 Key it's possible to change value of the standard concentration and carry out calibration against standard. Pressing F8 Key it's possible to change the factor and carry out tests against factor without any calibration. In case you choose F8 option, select factor and press F1 to go on with test.Calibration will run only for blank reagent O.D. reading, then it will go back on main men and will be possible to go into start analyzer option. When calibration is completed, the instrument goes back to main menu. NOTE* Calibrations must be executed before starting the routine, positions for standards are the same position for samples going from n. 44 to n. 58, when sample tray=58 is selected, and for samples going from 21 to 35 when sample tray=38 is selected, but normally these positions are used only in case of multistandard calibrations, in clinical chemistry the use of a single multistandard to calibrate more test is the most diffused, in this case beside the position of the standard is displayed an asterisk (*); when calibrations are completed, replace standards with sera if necessary. If a test is calibrated already, it will be prompted on the screen with an asterisk (*) beside to indicate it.
EXECUTE TEST TEST REAG. POS. REAG. VOL.ml FACTOR ............................................................................................................................... GLUCOSE R1250 15 >2 308.4 UREA R1414 7 8 2424.2 CHOLESTEROL 5 15 442.39 TRIGLYCERIDS 3 8 600 GOT/AST 2 10 1768 GPT/ALT 4 10 1768 CREATININE 11 >2 113.51 N. 6 6 8 6 6 6 6 67
Page 18 of
By pressing F1 or F4 you can start operations either in random or batch mode, by pressing option F5 it's possible to select which test should be executed, by pressing F7 the instrument is paused to change sample tray to be executed. Fill the reagent containers with the amount of reagent needed and place them in the proper positions in the reagent tray. NOTE - When the Reagents level detector is activated, in Maintenance Menu, and the function CK INITIAL VOLUME is enabled, before starting the operations, the instrument will always check all the reagents for the tests to be performed and will display directly on the screen the existing volume of reagent. In this case the operator is not allowed to type any number into the VOLUME field. In case the reagent wont be enough to perform all the existing samples, then a symbol > will appear beside the reagents volume and the instrument will not start the routine. Then it will be necessary to add reagent or if this is not possible, to deselect the test whose reagent is not enough.
At the moment we select the option F1 or F4 to start the routine, the screen will display the following :
PATIENTS ANALYZER UTILITIES HELP 12-06-1997 Esc=Exit
EXECUTE TEST TEST REAG. POS. REAG. VOL.ml FACTOR ............................................................................................................................... GLUCOSE R1250 15 10 308.4 UREA R1414 7 8 2424.2 CHOLESTEROL 5 15 442.39 TRIGLYCERIDS 3 8 600 GOT/AST 2 10 1768 GPT/ALT 4 10 1768 1) Check water container is full 2) Check waste bottle is empty 3) Place Reagents into reagent compartment 4) Place Samples, Standards and Controls into positions N. 6 6 8 6 6 6
5) Check the probes of Sampler and Aspiration system 6) Check if segments have been inserted !! OPERATIONS TIME hh:mm START ANALYSER Now, by pressing the ENTER key, the routine will start. PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
0:21
Page 19 of
67
During all the routine operations the screen will display the following mask: ANALYSER IN OPERATION SAMPLING GL - PAT. 5 GOT AZ GL AZ PAT. PAT. PAT. PAT. 1 1 1 2 I.D.: I.D.: I.D.: I.D.: 1st O.D.: 1.2524 FIT: 1.00 O.D.: 1.1021 1.0240 O.D.: 0.2421 O.D.: 0.9521 0.9012 READING GOT- PAT. 2 RES. : 15 RES. : 52 RES. : 150 RES. : 40 P P
F1=ENTER PATIENTS F3 = REAGENT BLANK F5=REPEAT F6=REPEAT WITH DILUTION F7=REPORT PRINTOUT F8=STOP ON PAT. N. F9=MENU F10=ADD REAGENT Alt+F1=Abort
On the screen the function being executed on each moment is being displayed immediately. On the lower part of the screen, we have several options that can be executed without stopping the routine in course. These options can be called and executed while the instrument is operating, as the system works in time sharing, so we can enter new patients to be executed in random mode, as patients, urgencies (stat) or controls, we can program repetitions with F5 and F6, we can stop the operation at a certain patient (i.e. pat. 8) by pressing F8.With F7 is possible to select a normal printout, a screen dump during routine work, or a REPORT PRINTOUT PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 20 of
67
F3 = REAGENT BLANK
F5=REPEAT
F6=REPEAT WITH DILUTION: With this key we can select a test and a sample to be repeated with dilution. In this case the dilution factor used is the one programmed in the test parameters, the dilution is performed by changing the Reagent/sample ratio and result is automatically multiplied by the dilution factor. F7=NORMAL PRINTOUT : This is a toggle function, it can switch between NORMAL PRINTOUT and REPORT PRINTOUT. In normal mode we have the printout of all the informations related to the test, as absorbance, FIT and so on. In report mode, we have a complete report patient by patient, with patient name, units, normal range and all the other informations normally written on a report. : During normal routine of the instrument, this function allows the operator to select a sample on which the routine will stop. Then, when this sample is reached, we can press ENTER to restart the routine. In case we add some reagent with function F10, because the system has calculated there is no enough reagent, i.e. for GGT, normally the system halt the sampling. Once reagent is addedd, the key changes into F8=CONTINUE, by depressing it the sampling for that test is restarted. : With this key we can go back to the main menu, once the routine has been completed, without stopping in stand by mode.
Page 21 of 67
F8=STOP ON PAT. N.
F9=MENU
WARNING GGT-NOT ENOUGH REAGENT And the system will stop sampling for that test, then operator must press the key F10,the instrument asks which reagent must be added (GGT) and the amount of reagent added, on the lower part of the screen F8 option changes to F8=CONTINUE, at the moment that reagent for GGT has been added, operator must press F8 key to continue sampling for that test. NOTE - When the operator wants to execute for a sample, a test which has not been previously selected, its enough to press the key F5, the instrument will ask for the test to be repeated, then the operator must enter the test he wants to add for that sample and the number of the sample. The instrument will add the test to the patient automatically. The only necessary condition is that the test requested must be already calibrated, otherway the system will not accept it.
2.7
At the end of the routine the PRONTO Evolution produces an acoustical signal to draw the attention of the operator. This signal lasts for a few seconds, then is silenced automatically and the instrument stays in stand by mode, waiting for new samples or repetitions or controls (Continuous Access Mode): ANALYSER IN OPERATION SAMPLING WORK COMPLETED GOT PAT. 8 AZ PAT. 10 GL PAT. 10 I.D.: I.D.: I.D.: 1st O.D.: 1.2524 FIT: 1.00 O.D.: 1.1021 1.0240 O.D.: 0.2421 READING RES. : 15 RES. : 52 RES. : 150
P P
F1=ENTER PATIENTS F3 = REAGENT BLANK F5=REPEAT F6=REPEAT WITH DILUTION F7=REPORT PRINTOUT F8=STOP ON PAT. N. F9=MENU F10=ADD REAGENT Alt+F1=Abort
At this point, basing on results obtained, we can decide to run some repetitions of tests with or without dilutions. To repeat these tests whose results are not affordable, select option F5 or F6 in case of repetition with a dilution (Remember that dilution factor is that preprogrammed in the method ), the screen will display the following : PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 22 of
67
P P
How to repeat: - pressing F5 or F6 key, the instrument asks for Test to be repeated and sample number. - type the test code and the sample number to be repeated followed by ENTER. The instrument will start the repetition of the sample immediately. - in case repetition with dilution is requested type the F6 key and proceed as described above. Note that all numbers of sample will report the flag indicated in routine beside results, as L or P or *, to indicate their necessity to be repeated with dilution. Dilution factors as programmed for the chemistry in question will be used. Dilution in this case means e.g. if normally 10l of sample are used, and the dilution factor equals 4, the sample will be repeated with 2.5 l. Once all repetitions have been performed and/or all the samples have been completed, press F9 key to return to the main menu. The system will perform some rinsing of the sampling and of the aspiration systems and then will stop giving an acoustical alert and displaying the following screen : ATTENTION !! Remove the used segments : From 1 to N YES NO
N represents the last used segment. If we replace all the used segments, also if not all the reaction wells have been used, then we select YES. In case the instrument didnt use all the wells of the last segment, then we can select NO. In this case the instrument will keep in memory the last reaction well used and will start over from the next immediately available. In addition to the repetitions inside the routine, it's possible to repeat tests and samples directly from the main men, by selecting the option REPEAT TESTS from the Analyzer Menu, in this case it's just necessary to select the test and the samples to be repeated and specify if the repetition must be simple or with dilution. The mask displayed is the following : PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 23 of
67
NUMBER OF PATIENT : F1 : delete result and continue; F8=All (Toggle : Insert/Remove) To dilute and repeat type (d or D) after no. of patient
To select the patients to be repeated, type the number of patient as listed in the upper part of the screen and press Enter to select it. Consider that performing this operation once will select the patient, performing it twice will deselect it (Toggle mode). Once a patient is selected its number will appear highlighted. To select a repetition with dilution, type the number of the patient followed by a d or D, (i.e. 5d or 5D) then press enter, in the upper part of the screen the number followed by a d will appear highlighted.Then once all patients to be repeated have been selected, press F1 to perform the repetitions. *STAT (Emergencies)* As explained in section 2.1 we can estabilish at the moment of entering a patient if its execution must be normal,a control or a Stat.In case we enter the patient with the "label" Stat, obtained by pressing F2 key, this sample will be executed with priority over the other samples.When we work in random mode, we can however enter a new patient with Stat option, pressing F2 key (Enter Stat) whenever we have this necessity.The normal routine never stops and it is possible to execute urgencies as normal part of routine.
2.8
The first level at which the results must be verified is on the test protocol that is being printed during the analysis. This check may lead to the conclusion that complete tests has to be repeated when e.g. the standard or the reagent wasn't good. Also samples may be detected that have to be diluted and then repeated. The option MANUAL RESULTS of the PATIENTS-menu serves two purposes: 1. To view the results before printing patient by patient and scrutinize them for clinical plausibility . Modifications can be made. 2. To enter the results of off-line chemistries, i.e. chemistries that are not determined on the PRONTO Evolution, like e.g. Na and K. This way a more complete test report can be achieved.Typing this option the following choice is displayed:
ENTERING MANUAL RESULTS
Page 24 of
67
By selection of DELETE RESULTS item, the instruments displays the following mask:
DELETE RESULTS EXISTING PATIENTS: 4 First Patient n. : Last Patient n. :
You are requested to enter the first patient and the last patient numbers you want delete results, then type ENTER key to confirm and all patient numbers indicated will be deleted. Note that every time a repetition is performed, old results are deleted automatically. Otherway if option ENTER RESULTS is selected, the following choice will be displayed:
ENTERING MANUAL RESULTS BY PATIENT BY TEST
Number of patients will display how many patients are present and in the lower left part of the screen must be entered the number of the patient whose we want to enter or modify the results.Once ENTER key is pressed to confirm the patient whose results we want to modify, on the screen will appear the following mask:
ENTERING RESULTS - 2 PATIENT ID : NAME :
GLU : 112
GOT : 24
BUN : 39
Page 25 of
67
If instead of the option " Enter results by Patient", you choose BY TEST, on the screen will appear :
ENTER MANUAL RESULTS
EXISTING PATIENTS : 4
The operator has to select the range of pat.numbers in which he wants to enter the results.After this on the screen will be displayed the following:
ENTER RESULTS - TEST ID ?
Type in the box the test-ID you want to modify or enter results. The patients are displayed one after the other and the results can be typed in as displayed below:
N. 2) 3) 4) PAT. ID PAT. NAME 002 BROWN 003 SMITH 004 PORTER TEST RESULT GLU : 95 GLU : 125 GLU : 300
When ready go back with ESC. This option offers the possibility to delete results without deleting the tests requests. It may be useful after a run with bad results.
2.9
REPORTING RESULTS
Results can be printed in two ways: - as patient reports PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 26 of
67
Selecting ths option REPORT RESULTS on PATIENTS MENU, the screen will prompt:
REPORT RESULTS OF TODAY OLDER DATES
Selecting the option OLDER DATES will be possile to print reports of patients stored in the long time storage memory (Floppy Disk drive or H.D.D.), the screen will show all preceeding dates present on the disk:
PRECEEDING DATES 20-06-95 22-06-95 23-06-95
then it's necessary to select the date we are interested in , on the screen will be displayed the following request:
the option PRINT will display the criteria of selection of the patient, (refer to report results for today). The option VIEW will display all patients presents in that date with ID, NAME and tests associated:
NUM. 1) 2) 3) 4) 5) 6) 7) 8) 9) 10) | | | | | | | | | | | ID. 001 002 003 004 005 006 007 008 009 010 | NAME | P. Smith | A. Red | | | | | | | | TEST - PATIENT N.: 2 GLU = 95 CHO = 165 BUN = 25
Page 27 of
67
REPORT RESULTS - From N. Till N. - Selectively by number - All - By name - By Patient ID.
then enter the number of the first patient and the of the last patient whose reports must be printed and push ENTER;the print will start for all patients included in the defined range. In the same way we can select the patient whose report we want by typing the patient number, the name or the ID.Of course selecting ALL, we will print all patients of today reports.The same selection mask will be displayed also when we choose PRINT in OLDER DATES reports program. PRINTING RESULTS PER TEST With this option of the PATIENTS-menu the results can be listed for the individual tests.The first option mask that is displayed on the screen is:
Selecting ALL TEST option, all test present will be printed, selecting CHOOSE TEST, will be displayed the Test Block Mask, where we will be able to choose test to be printed, using the arrow keys or the capital letters code as explained in the Calibration section. PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 28 of
67
2.10
PREDILUTING SAMPLES
the the the the the
The option PREDILUTION of the ANALYZER-menu offers the possibility to use PRONTO Evolution to predilute automatically samples.It's important to note that Predilution function is to be used for all samples that need to be diluted before analysis, as the urine samples and serum samples for some immunochemistry tests. This dilution is not to be confused with the post dilution function performed by instrument in the repetition with dilution function. Selecting predilution function on Analyzer Men, you enter in a screen which asks for the following data:
PREDILUTION Number of Samples (1 to 6) : Sample volume (l) Diluent volume(l) : :
First enter all parameters requested,remember that the max quantity of reaction mixture that can be contained into the reaction vessels cannot exceed the amount of 800 l. Then hit F1 key and will be prompted another screen in which is possible to modify the sample/diluent volume ratio sample by sample, i.e.:
PREDILUTION DILUENT POSITION (1-15) : 1 DILUENT VOLUME l SAMPLE VOLUME l 1) 200 200 2) DILUENT VOLUME l SAMPLE VOLUME l 150 250
hitting F1 again will be prompted operation mode and all checks to be done before operation starts, then press Enter to execute predilution. Place up to 29 samples into the odd-numbered positions: 1, 3, 5, 7, ........., 57. Place empty sample cups into the even-numbered positions: 2, 4, 6, 8, ...........58. Sample 1 will be diluted into cup 2, sample 3 into 4 etc. PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 29 of
67
2.11
QUALITY CONTROL
This option of the ANALYZER-menu offers 2 sub-options when we are running the software from the floppy disk : MONTHLY QC CALCULATE
In case we are running the software fron an hard disk drive C:\, we will have these options : MONTHLY QC CALCULATE KINETIC CURVES
Options related to the Quality Control Program are the following : - MONTHLY QC, statistics and graphics on values stored on daily basis. - CALCULATE , a simple CV-program for values to be entered manually. When we select the option MONTHLY QC, we have the following choices : MODIFY SD DELETE MONTH
MODIFY : For both 2 controls of each test an average value can be entered for each day of the month. S.D. : Mean, S.D., and C.V.% are calculated over the values stored automatically in the routine. DELETE MONTH : All values of the month can be deleted, either for all chemistries at the same time, or for individual chemistries. It is important to know that only if controlsera C1 and/or C2 are programmed in the methods of the test, will be performed during the calibration and the associated results will be stored in the monthly QC calculation memory, to perform this function, running controlsera in the normal routine, gives only a simple indication if the controls are within the estabilished range, but will not be used for the monthly QC calculation.
Page 30 of
67
CALCULATE S.D.
35 35 36 35 36 34 35 34 35 35 34
36
It's just necessary to type the values and press ENTER after each value, in case we want to remove an erroneous data, we have just to type it with the minus (-) sign before (i.e. -16) and entering it the wrong value will be deleted. Once all values have been entered, press ESC key, on the screen will be prompted the following: CALCULATE S.D.
35 35 36 35 36 34 35 34 35 35 34 36
0.674
C.V.
1.95%
By depressing the F9 key, you'll print the numerical data of the SD and CV, by pressing F1 key, a Levey-Jennings chart will be visualized:
GOT - GOT/AST QUALITY CONTROL - GOT/AST CONTROL SERUM : 1 + + + 10 15 20 25 30
250 200
+ ++ 150 + + + + 1 5
+ + + + +
++ + +
F1=CONTINUE
F9=TO PRINT
In the same way, by depressing F9 it's possible to print the Levey-Jennings chart.
Page 31 of
67
0 25 F1=CONTINUE F5=PRINT
By pressing F1 its possible to display the curve of the next patient for the same test, by pressing F5 its possible to obtain a printout of the curve. Once all the curves have been displayed, the system will ask again whose test the curves are to be displayed. To return to the QC menu, at this stage press ESC.
2.12 IMMUNOCHEMISTRY
PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 32 of
67
RUN TEST will perform the routine of immuno, CALIBRATION will perform the single or multi point calibration of the immuno test and the last option, VIEW AND PRINT CURVES, will display and print on an external printer, the last calibration curve for each test of immunochemistry. Of course the calibration of each test of immuno, must be performed before running the test with samples. To perform the calibration of the tests requested, simply select the option CALIBRATION. On the screen will appear :
CALIBRATION TEST IMMUNOCHEMISTRY
A) F) K) P) U) Z)
B) G) L) Q) V)
[0 [0 [0 [0
] ] ] ]
[0 ] [0 ] [0 ] [0 ] [0 ]
D) I) N) S) X)
] ] ] ] ]
F2 = VIEW
So far the steps and the functions available are exactly the same we can have in the General Chemistry calibration menu. Beside each test is indicated the number of samples to be executed. Once tests to be calibrated have been selected, press F1 key to start the calibration routine. NOTE - Its always necessary to run a new calibration whenever a new Antiserum lot is used.
Once F1 key has been depressed, on the screen will appear the following :
PATIENTS ANALYSER UTILITY HELP 12-06-1995 Esc = Exit
Page 33 of
67
F1=Continue
Press F1 to continue, then will appear the request if the operator wants to deduct predilution of the calibrator. Thi is useful when a single calibrator is available, in this case this function allows the operator to build a calibration curve automatically using a single calibrator only.
PATIENTS ANALYSER UTILITY HELP 12-06-1995 Esc = Exit
CALIBRATION IMMUNOCHEMISTRY TEST TEST IgA IgG IgM REAG.POS. 15 15 15 2 3 4 REAG.VOL. ml 10 10 10 5 5 5 LOT. DATE OF LAST CALIB. 2254 2705 1152 24/04/97 24/04/97 24/04/97 N. CAL. 4 5 4
By selecting YES, on the screen, below the Reagents positions, will appear the diluent position, normally 15 common to all the tests, then the instrument will give the instructions to proceed for the predilution.
DEDUCTING PREDILUTION
PREDILUCTION - ATTENTION Place: Diluent in Position : 15 Calibrator in Position : S6 Empty cups in the Positions : (S1 - S5)
Page 34 of
67
START
F1=Continue
Perform all instructions indicated on the screen and press ENTER, the instrument will execute an automatic dilution of the calibrator in position S6 in the empty cups from S1 to S5 according to the following ratios : 1:2 - 1:4 - 1:8 - 1:16 - 1:32 to build the necessary calibration curve. The diluent container is always 15 for all the tests. Once the curve has been built, the instrument will perform the autozeroing of all the filters with distilled water and then will proceed to the calibration in the following order of process : 1. CALIBRATORS PREDILUTION ACCORDING TO THE METHOD 2. SAMPLING OF THE CALIBRATORS BLANK ( Prediluted): Calibrator + R1 Incubation Reading 3. SAMPLING OF THE CALIBRATOR ( Prediluted ): Calibrator + R1 + R2 ( Antiserum ) Incubation Reading 4. RESULTS CALCULATION
Once the instrument has completed the reading of all the calibrators selected, on the screen appears the calibration curve obtained with the mathematical model (Algorythm) used, the given concentration and the recalculated one for each point of calibration., with the Delta % between the given concentration and the recalculated one, flagged if out of the specified maximum value allowed as programmed in method.
IGA - IGA
Rev. 3 - 12/98
MODEL (1-6) : 1
5 4
Now , before storing the curve in the memory, its possible to modify it by changing the mathematical model or deleting one calibration point, this in order to achieve the best fitting of the curve with the minimum Delta between the given and calculated concentrations. Once the curve is satisfactory, press F1 to store it.
SECTION 3. 3.1
PROGRAMMING CHEMISTRIES
Up to 50 chemistries can be programmed on one diskette. To program or modify a method, select the option METHODS PARAMETERS from the ANALYZER-menu. There are 3 sub-options, wich can be selected with the cursor keys and the ENTER-key: PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 36 of
67
MODIFY METHODS
PRINT METHODS
REARRANGE METHODS
With the option MODIFY METHODS, it's possible to modify or create your own methods for clinical chemistry, immunochemistry one point and immuno multi point, with the option PRINT METHODS, you can print a single test's parameters or all test's parameters, finally, with the option REARRANGE METHODS, it's possible to modify the visualization order of all tests.
3.2
With this option we can select the class of the test to be modified or creted, we have displayed three choices:
By selecting the CLINICAL CHEMISTRY option, we enter in the parameters mask, were, moving the cursor on all the various fields using the arrow keys and the ENTER key to confirm the data entering, we can modify the various parameters to define a clinical chemistry test.
These are the parameters we have: TEST-ID: - if an unknown test-ID is entered(3char.),the program asks you to confirm if a new test is to be made. - if a known test-ID is entered, the program allows you to change the ID or leave it as it is.Subsequently all parameter fields are being displayed. - if a test-ID is selected that already has been assigned in another class, the program will warn the operator and ask for confirmation : 0: Off-line test, not be analyzed by the ELECTA.This offers you the option to collate tests like Na or K into the report.
Page 37 of 67
No.of washes aspirator(2-4) : - (at the end of the test) NOTE: In case of typing errors, use BACK SPACE to return to the beginning of the list without storing the modifications. If a modification has been made in the middle of the list, use ENTER to store the modification and to exit the method.
3.3
PRINT METHODS
By this selection there are two options of printing tests: PRINT ALL :To obtain a printout of all programmed chemistries with parameters
PRINT SELECTIVE :Individual tests can be selected. After the selection hit ESC to start printing.
3.4
This option displays the mask with all programmed tests: TP BUN CHO GOT TRI GPT GLU 1 2 3 4 5 6 7 GGT CRE FAC CHE LDH FAL BIT 8 9 10 11 12 13 14 BID UA CA FE AMY CL HDL 15 16 17 18 19 20 21 CPK PH MG FAP PHL BIL GUV 22 23 24 25 26 27 28
By entering a number beside each test code, it defines the test's position on the screen arrays and so it offers the possibility to change the sequence of the methods, without having to enter all the parameters from the beginning. PRONTO Evolution USERS MANUAL Page 39 of 67
Rev. 3 - 12/98
3.5
CONFIGURATING PROFILES
Profiles are groups of tests that can be composed by the user and serve to speed up the manual entering of test-requests. With the option PROFILES of the UTILITIES-menu up to 9 profiles can be selected (19). Each profile can have as many tests as there are in the test file.
3.6
The heading of a patient report consists out of 4 lines, that can be adapted to the laboratory in question. Select the option REPORT-HEADER of the UTILITIES-menu. The program offers the option to just VIEW the header (without danger to change it accidentally) or to MODIFY. In case of MODIFY: Select the line to be modified with the UP and DOWN-cursorkeys. Use the other cursor keys and the Back-Space to modify the line.
REPORT HEADER LABORATORY *********************************************************** BPC BioSED srl T.sa del Grillo Km 0.6 - Castelnuovo di P. - Roma ***********************************************************
3.7
CONTROL SERA
With the option CONTROL-SERA of the UTILITIES-menu the assay values of the control sera can be entered. Once selected , this option asks if we want to DEFINE the control sera values or we want to PRINT them. Selecting either DEFINE or PRINT, the instrument offers the choice for SELECT TEST or ALL TESTS. The choice SELECT TEST, will visualize the test code block, as in calibration mode, then we can select all tests we want to modify the values of the control sera.Once all test required are selected, press the ESC key to continue, will e displayed the following:
ENTERING VALUES OF CONTROL SERA TEST : GPT CONTROL 1 CONTROL 2
Rev. 3 - 12/98
With the option ALL TESTS the upper and lower limits of the 2 control sera of the first chemistry in the list are being displayed and can be modified.
after which the table of the PRONTO Evolution tests is shown. In the left column the PRONTO Ev. Codes may be entered, in the right column the communication ID's, for example: PRONTO Code GOT GLU GLU GLU GLU LDH COMPUTER Code GOT GLU8 GLUpp GLU12 GLU16 ... etc.
Page 41 of 67
With the options 1 and 2 of the UTILITIES-menu the date or the time can be adjusted. As the computer has got a battery operated clock on board, the date and time should always be right after switching on and off.
3.10
DISK COPIES
With these options, COPY DISK and COPY PATIENTS ON DISK, its possible to perform several operations involving the floppy disk drive. The option COPY DISK, allows the operator to copy from a program disk onto another new program disk, all the methods and the settings of the source disk. Its important that the target disk is a brand new software disk. The option COPY PREVIOUS PATIENTS is available only when the software is installed onto an hard disk, and allows the operator to copy patients from a floppy disk into the hard disk, to filed for future uses.
SECTION 4.
Before, during and after the routine the PRONTO Evolution rinses according to a programmed schedule probes, syringe, tubing and flow-cells and also checks the interference filters. The operator has however the possibility to: start a complete rinsing and checking cycle as a daily maintenance execute one specific step of this cycle in case a certain part of the system is suspect
The option MAINTENANCE of the ANALYZER MENU offers the following suboptions: SELECT SAMPLE TRAY (38/58) = 38 ADJUST PERISTALTIC PUMP CHECK ASPIRATION SYSTEM PRIME DILUTER WASHING CUVET CHECKING FILTER (Autozero) POSITIONING FILTER / O.D. READING USE OF PRINTER - ADJUST DAILY MAINTENANCE PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 42 of
67
Page 43 of
67
The normal function AUTOMATIC rinses the flow cell with cleaning fluid. Selecting the AUTOMATIC mode, we have other 2 options : WASHING CUVETTE AUTOMATIC MANUAL
In automatic mode water (or eventually cleaning agent) from water container is deposited into the washing well, where is heated, and it is aspirated through the flow-cell. In MANUAL mode the system will prompt only to activate the peristaltic pump. In this case its necessary to extract the aspiration probe from the aspiration arm, dip it into a vial containing the solution we want to aspirate into the flow cell and activate the peristaltic pump. With the option WITH IPOCLEANING AND EXTRACLEANING, the system demands to put the cleaning solutions in Reagent Positions 1 and 2, then perform the maintenance cycle automatically. Do not forget to perform another rinse cycle after the maintenance cycle.
4.8
MAINTENANCE
Daily Maintenance Although the system is rinsed automatically during the routine, the operator should apply an extra cleaning at the end of the working day.The option DAILY MAINTENANCE of the MAINTENANCE menu executes the rinsing of the sampler, the aspirator and flowcell and performs a filter check.Water must be present in the water container. Weekly Maintenance Once a week the system may be washed with a detergent or in extreme cases with an PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 45 of
67
active solution of Hypocleaning agent BPC.This wash can be executed in manual aspiration or with the automatic maintenance cycle in WASHING CUVETTE function. Quarterly Maintenance Once every 3 months it is recommended to replace the tubing of the peristaltic pump as described in 5.4. The exact interval depends on the workload of the laboratory and has to be determined by experience. For a complete maintenance schedule, refer to the page MAINTENANCE SCHEDULE of this manual, it's suggested to copy the page and to put the copy on the instrument or near to it, to have always an immediate reference for periodic maintenance.Consider that the Hypocleaning is a deproteinizing agent and the EXTRAcleaning is an acid Ph strong cleaner and degreaser.
4.9
PASSWORD(2)
With this option it's possible to lock the access to any men and sub-men of the PRONTO Evolution fixing a Password to allow the access to locked Items. To install this password, select item password(2) and press ENTER key, you are prompted to entry a 2 letter password, i.e. JR, then you can move on all items to be locked and when item is selected, press ENTER key to confirm access denied. When all menues to be locked have been selected, press ESC key to return to normal operations. Now to access to locked items, you are prompted to enter the Password. In case it is necessary to delete the password, proceed as follows: select again password (2) item in maintenance men, then enter the password, i.e. JR, then system will prompt the choice:
MODIFY DELETE
MODIFY DELETE
: will ask you a new password to protect items already selected : will delete completely the password and selection made of items to be protected
4.10
This option of maintenance menu, allows you to change via software the serial port 1 or 2, on the computer of the instrument, to be connected to another lab computer for on line data transfer.When selected, this option just asks for COM 1 or COM 2, you have only to select which one you choose and hit Enter key to confirm.
4.11
This is a toggle function. When beside this item there is the indication YES, every time PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 46 of
67
4.12
On this item you can switch to on or off the water level detector, connected to the water container if presetted for this use. If your water container is provided with the level detector, connect the jack plug to the socket located on the right side of the PRONTO Evolution, just above the water input nipples.With this option you can choose if you want the level detector activated or deactivated.
4.13
The PRONTO Evolution is provided with a cooling system for the reagent compartment. This cooling system is based on Peltier elements and is electronically controlled to switch between two differents adjustable temperatures. These temperatures are factory set at the same level, but its possible to adjust them refering to the Service Manual - Reagents Cooling Temperatue adj. section.
The cooling system provides a temperature which is max 10C less than environmental temperature. The switching between the two temperatures is provided via software in the Mechanical Test menu. Its a toggle function and the only visible effect of this operation is the change in the colour of the LED lamp located on the left side of the instrument : when its green colour the temperature selected is the n. 1, when its yellow colour the temperature selected is the n. 2, when its red the cooling system is off. To switch on the cooling system use the cooling unit switch whch is located on the rear of the instrument, near to the main switch. NOTE - Never switch on the cooling system while the unit is already switched on or its operating, as this can cause malfunctionings or more severe damages. Always switch it on before switching on the instrument.
4.14
TROUBLE SHOOTING
Page 47 of
67
Page 48 of
67
Sample Tray
RESET SAMPLE POSITION REACTION VESSEL FOR DISPENSATION REACTION VESSEL FOR ASPIRATION
Diluter
RESET LOAD DILUTER VALVE OFF VALVE ON : returns to home position : enter a number (ul) and push ENTER.The selected amount of reagent will be aspirated. : deactivates the valve : activates the valve on top of the syringe
Peristaltic Pump
WASH ASPIRATE
: the pump will turn like for : the corresponding function MANUAL Page 50 of
67
Total Reset Set and Store Steps Switch Reagents Temperature Level Detector Sensitivity Adjust. Reagent Volume Check Store Values of EEPROM on Disk
: refer section 4.16 : Toggle switch the cooling temperature : refer section 4.16 : perform a check of level detector : copy of values of EEPROM on disk
4.16
In PRONTO Evolution, all the mechanical and electronic settings to operate correctly, are stored into an EEPROM. This in order to reduce the necessity of the operator to perform any adjustment before operating the instrument. Due to different causes, as the connection of the instrument to its computer when this is already on, or any problem occurring on the power line, these settings on EEPROM can be damaged or partially erased. In this case its necessary to set once more the mechanics and the operating sets of the instrument. The same situation can be necessary if we replace a mechanical assembly, then its also necessary to adjust the steps to center the mechanics in relation one to each other. This operation must be performed every time we have the following alarm when we switch on the system : PROBLEM WITH EEPROM OR SOME SETTINGS MISSING
Page 51 of
67
4.16.1 Set and Store Steps Enter in Mechanical Test Menu and in Set and Store Steps function, sequentially will be displayed on the screen the settings to be performed. Use the + and - keys to set the positions forward or backward in simgle step mode, then press the Enter key, once the positioning has been completed, to jump to the next setting.
This is to center the position of reaction vessel under the aspiration probe
POSIT. OVER INNER SAMPLE PRESS + OR - KEYS POSIT. OVER OUTER SAMPLE PRESS + OR - KEYS
Page 52 of
67
Basically the same principle of positioning and centering as above described, but on the reaction vessels
Positioning of the sampling probe over the inner and the outer parts of the waste well
POSITION OVER REAGENT PRESS + OR - KEYS
Centering the sampling probe position over the reagents container ( Default position 1 )
HORIZ. MOVEMENT OF ASPIRATOR PRESS + OR - KEYS VERT. MOVEMENT OF ASPIRATOR PRESS + OR - KEYS
Horizontal and vertical positioning of aspiration probe over the outer reaction vessel
HORIZ. MOVEMENT OF ASPIRATOR PRESS + OR - KEYS VERT. MOVEMENT OF ASPIRATOR PRESS + OR - KEYS
Horizontal and vertical positioning of aspiration probe over the inner reaction vessel
Once this has been completed, press Enter key and the system will store the values on EEPROM and then return to Mechanical Test Menu. In case there are problems on the EEPROM and this need to be replaced, the following message will be displayed : ATTENTION : VALUE ON EEPROM HASNT BEEN STORED , TRY AGAIN OK In this case try again by pressing Enter. Considering that after a certain number of times the warning will disappear, check if the system operates correctly, if the mechanical settings have not been stored correctly or certain problems persist call the service PRONTO Evolution USERS MANUAL Page 53 of 67
Rev. 3 - 12/98
4.16.4 Other Settings Perform these other settings in the Maintenance Menu : Select the sample tray to be used Adjust the number of steps of the peristaltic pump in order to comply the following specifications :
Aspiration Probe
Check the if specifications are met using the Check Aspiration System option Select the printer to be used with the eventual settings related (i.e. Printout Intensity) Select the serial port to be used for the On-Line connection to an host computer Select wether or not perform the automatic repetition if beyond linearity Activate or deactivate the water level detector and the reagents level detector NOTE - Also if the setting is already the one wanted, select another item and then select again the one wanted, this in order to store it again.
SECTION 5.
5.1 DISASSEMBLING THE CABINET First of all it's always necessary to disconnect the main plug from AC outlet before performing any replacement or disassembling the cabinet of the instrument. To perform all replacements described into this section, will be enough to disassemble only the upper part of the cabinet. Proceed as follows: Pull up the printer ass'y from its housing and disconnect the printer cables Unscrew the two screws located on both sides of the upper cover Pull up the entire upper cover carefully to PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 55 of
67
Page 56 of
67
Remaining into Mechanical Test menu, select again other 500 ul into Load Dilutor item and let the moving bar descending more until it stops (be careful that maximum volume allowed for the Load Dilutor Function is 1000 ul, in case you exceed this volume, it can damage the dilutor module) Unscrew the syringe from its header block and pull down the syringe with the piston,at this stage the piston can be removed and replaced, be careful to the O' Ring sealing the head of the syringe. Repeat inverse items to reassemble the syringe into the dilutor, check always that the piston's lower edge must be overlapping the moving bar of about 8 mm, to avoid it can break the syringe head. To avoid this trouble it's really better to tighten the moving bar screw on the piston, only when you are sure it doesn't touch the upper inside of the syringe (reset position).
- switch off the instrument - Remove the cover of the photometer. - Disconnect the plug of the lamp from main PCB - unscrew the fitting of the lamp - pull out the lamp - put in the new lamp and assembly in reverse PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 57 of
67
adjust the potentiometer GAIN on the photodiode amplifier PCB, and repeat the ADC-reading until you'll have : VOLTS=8.5 push ESC to exit select OFFSET and hit ENTER, then again select READ ADC and press ENTER again, the offset value displayed must be between 0.0020 and 0.0060. (typ. 0.003),it can be adjusted with the trimmer marked OFFSET, after which the reading has to be repeated.
NOTE - It's not important that the filter of highest transmission (it can be the 340 or 620 it's not important), is perfectly set to 8.5 V, it's important that performing an autozero, all filter's voltages are within estabilished range of 3.5 to 9.5 V. It's suggested that the mean of the filters is far about 1-1.5 V from the extreme limits of the allowed range (3.5 9.5).
To replace the tubing: untighten the screw remove the old tubing put in the new tube press the leader by hand against the tubing
Page 59 of
67
NOTE - On the opposite side of the leader of the pump there is a locking plate, which is factory set in the correct position. This is necessary to avoid the leader can press too much the tubing leading to shorte life of the tubing itself. Never attempt to adjust this plate otherway problems can occurr.
APPENDIX 6-A
TYPES OF CALCULATION OF THE PRONTO Evolution The calculation types 1 - 5 can be used either with FACTOR or with STANDARD. Type of Calculation = 1 ENDPOINT Conc.Standard Conc.Sample = Abs. sample Abs. Standard Conc.Standard The Calibration Factor Abs. Standard I case of endpoint determinations with factor or with 'stored calibration' the formula becomes: Conc.Sample = Abs. sample F Type of Calculation = 2 KINETIC
Page 60 of 67
can be stored as F.
in case of decreasing (-) reactions: if the first reading < (RB - SE)
APPENDIX 6-A
Type of Calculation = 3 INITIAL RATE
This corresponds to fixed-time-kinetics. The calculation is based on the delta absorbance over the reading interval : one reading at the start (= end of incubation time) the second at the end. In this case we use the Abs. between 1st and 2nd Abs. instead of the simple Abs. Type of Calculation = 4 BICHROMATIC
The calculation is based on the delta Abs. between 2 wavelengths. Formula like in End Point. Type of Calculation = 5 SERUM BLANK
This type of calculation is based on the delta Abs. of a sample with reagent and a serum blank (sample + diluent). To this end 2 reaction wells are being used for each sample. Calculations as for Endpoint. For the calculation of the non-linear standard curves the operator may choose out of 6 mathematical models (Algorythmes). PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 61 of
67
MODEL 2:
5-Parameter Log-Logit 1
R = R0 + K c 1 + exp [-(a+b.lnC+c.C)]
APPENDIX 6-A
MODEL 3: 5-Parameter Exponential
MODEL 4:
ln C = a + b
MODEL 5:
4-Parameter Logit 1
R = R0 + K c 1 + exp [-(a+b.C)]
MODEL 6:
C = a + b.R
Page 62 of
67
Page 63 of
67
STRING FORMAT each data string consists of : STX .... data.....BCC ETX The data consist of readable ASCII characters only, between ASCII 32 to 255. The BCC consists of 2 ASCII characters, representing the sum of the characters not including STX and BCC and ETX, expressed in hexadecimal notation. BCC = ? (ASCII characters) % 256 (%) It is modulus or remainder operator
Page 64 of
67
request string
if not acceptable resend on NAC next string on ACK or EOT if no more strings.
ACK NAC
if string accepted
If the PRONTO Ev. wants to send results to the computer, it first sends an ENQ to establish the connection. The computer should answer with an ACK. If no answer is received, the PRONTO Evolution sends an ENQ every 10 sec. COMPUTER PRONTO Evolution (transmit mode)
ENQ (connection established) result string if acceptable if not accept resends on NAC next string on ACK when finished
EOT
APPENDIX 6-B
PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 65 of
67
APPENDIX 6-C
PRONTO Evolution USERS MANUAL
Rev. 3 - 12/98
Page 66 of
67
FC CK CK
ERROR MESSAGES ON SCREEN WARNING PROBLEM ON EEEPROM - SOME SETTINGS MISSING Check all the settings as described in section 4.16 or replace the EEPROM. WARNING COMMUNICATION PROBLEM WITH CONTROLLER 1 (2, 3) Problems of motor or electronic controllers.(Refer service manual, contact your dealer) FILTERS OUT OF RANGE Check if lamp is burned out, flow cells dirty or clogged, in case this message does not disappear, contact your dealer.
Page 67 of
67