Phng php xc nh hm lng ng tng s trong thc phm 1.

Nguyn l Lng ng c th c xc nh bng phng php th tch bng cch s dng dung dch kim ng sulphat, cht ny c cc loi ng kh thnh oxid ng mu . Quy trnh bao gm vic xc nh th tch ca dung dch ng cn kh mt khi lng dung dch Fehling bit trc th tch bng s dng xanh methylen lm ch th. Khng kh c loi tr khi hn hp phn ng bng cch un cht lng si trong sut qu trnh chun . Nhng loi ng khng kh nh l saccarose cn phi c thy phn thnh ng kh trc khi chun . 2. Thit b, dng c - My khuy t gia nhit, ni cch thu, t hood c. - Buret, pipet bu 10ml,50ml, bng thu tinh, bnh nh mc, ng ong, bnh cu, phu thu tinh. 3. Ho cht - Dung dch NaOH 50% - Axit HCl tinh khit - Dung dch kali ferocyanid 15%: Cn 15 gam kali ferocyanid thm nc ct va 100ml. - Dung dch km acetat 30%: Cn 30 gam km acetat thm nc ct va 100ml. - thuc th Fehling gm: + Thuc th Fehling A: CuSO4 tinh th : 40 gam Nc ct va : 1000 ml Lc k cho tan ht, nu khng tan th cho thm 1 ml H2SO4 v lc k. + Thuc th Fehling B: Kali Natri tartrat : 200 gam

NaOH : 150 gam Nc ct va : 1000 ml Ho tan 200 g Kali Natri tartrat trong 400 500 ml nc ct. Mt khc ho tan 150 g NaOH trong 200 300 ml nc ct. Trn hai dung dch vi nhau v thm nc ct va 1000 ml. Khi dng ly 25 ml dung dch Fehling A pha vi 25 ml dung dch FehlingB. 4. Cch tin hnh 4.1 X l mu Cn khong 1 5 g tu loi thc phm vo bnh nn 250 ml. Thm 45 ml nc ct v 5 ml acid HCl ri un cch thu trong 3h. Sau lm ngui di vi nc lnh. Cho khong 3- 5 git phenolphtalein v trung ho bng NaOH 50% n mu hng. Chuyn ton b dch ny vo bnh nh mc 100 ml. Thm 15 ml Kali ferocyanid 15% v 15 ml km acetat 30% lc u thm nc ct va 100ml, lc u ri lc trn giy lc. 4.2 Xc nh hm lng ng - Pha dung dch chun ng Glucose 1%: Cn chnh xc 1,0000 g pha v a vi 100 ml nc ct. - Chun li Fehling : Cho dung dch ng chun ln Buret.Ht 10 ml Fehling vo bnh nn 100 ml thm 4 git xanh methylen 1%. un si dung dch 3 pht ri nh t t ng trn buret vo hn hp ang si n khi mu xanh bin mt. Ghi s ml ng - Thc hin lm mu tng t nh chun thay dung dch chun ng bng dung dch mu lm mc 4.1 5. Tnh ton v nh gi kt qu Nng ng trong dung dch c th xc nh c bng cch s dng cc gi tr sau: 1 ml dung dch Fehling = 4,95 mg glucose = 5,25 mg fructose

= 5,09 mg ng nghch chuyn (invert sugar) = 7,68 mg maltose = 6,46 mg lactose = 4,75 mg sucrose 49,5 x 250 % ng tng (tnh ra glucose) = --------------------------------------------------------------TxWx10 Trong : T: th tch (ml) ca dung dch ng khng thu phn dng W: trng lng mu thc phm c s dng (g)