Professional Documents
Culture Documents
ELISA TEchnique
ELISA TEchnique
ImmunoSorbent Assay
TestWashing
specimen
willisremove
added, excess
which may orbounded
or un may not contain
Ag
the antigen
Sample 1 Sample 2
An enzyme-labeled
Washing will
antibody
removespecific
excess to
Conjugate
the antigen is
)added )conjugate
s s
s s
s s
s s s s s s
Sample 1 Sample 2
Chromogenic
Stopping substrate
solution isisadded
added,towhich in the
stop any
.presence of the enzyme,
reactionchanges its color
Optical Density )color)
Concentration of Analyte
The amount of color that develops is proportional
.to the amount of antigen in the test specimen
Sample 1 Sample 2
Test
Washing
specimen
willisremove
added, excess
which may
serum
or may
with not
un bounded
contain the
Ab antibody
Sample 1 Sample 2
An enzyme-labeled
Washing will remove
antibody
excess
specific
or un
tobounded
the serum
)antibody isLabeled
added Ab
)conjugate
s s
s s
s s
s s s s s s
Sample 1 Sample 2
Chromogenic
Stoppingsubstrate
solution is added
added,to
which
stop in
anythe
reaction
presence
.of the enzyme, changes its color
Optical Density )color)
Concentration of Analyte
Sample 1 Sample 2
Chromogenic substrate
Stopping solution is added,
is added which
to stop in the
any
.presence of the enzyme, changes color
reaction
Optical Density )color)
Concentration of Analyte
Competition
An enzyme-labeled to remove
bindtracer
antigen
Washing will to attached
any)conjugate)
un
Ab antibody
specific to the attached
bounded Ag is added
after with serum
s s
s s
s s
s s s s s s
Sample 1 Sample 2
Chromogenic
Stopping substrate
solution isisadded
added,towhich in the
stop any
.presence of the enzyme,
reactionchange its color
Optical Density )color)
Concentration of Analyte
Washing Antibody
An enzyme-labeled
Competition will remove
to bind any
tracer
to unAg
)conjugate)
attached
specific to the attached antigen
bounded Agare added after
with serum
s s
s s
s s
s s s s s s
Sample 1 Sample 2
Chromogenic
Stoppingsubstrate is added
solution is added,towhich
stop in
anythe
.presence of the enzyme,
reactionchange its color
Optical Density )color)
Concentration of Analyte
Competition
Serum is addedbetween
Washing the
will it
which serum
remove
may anyAb andto
unAb
contain
coating
boundedAbAgto tracer
bind toSol
.Neutralizing Ag serum
and tracer Ab
Sample 1 Sample 2
An enzyme-labeled antibody
Washing will remove specific
excess to the
or un
)antigenbounded
tracer is Labeled
added )conjugate
Ab
s s
s s
s s
s s s s
s s
Sample 1 Sample 2
Chromogenic
Stopping substrate
solution isisadded
added,towhich in the
stop any
.presence of the enzyme,
reactionchange its color
Optical Density )color)
Concentration of Analyte