CHAPTER 5 SUMMARY, CONCLUSIONS, RECOMMENDATIONS

SUMMARY This research is all about the study of the capability of sampaguita ethanolic extract to produce a zone of inhibition. After several trials the researchers came up to the summary. Extract from sampaguita flowers was prepared to be used for testing its antimicrobial property against Staphylococcus aureus and Escherichia coli. Tetracyline and Chloramphenicol were used as positive control for Staphylococcus aureus and Escherichia coli, respectively. The said bacteria were obtained from Jose Abad Santos General Hospital. The antimicrobial activity of sampaguita-ethanolic extract was studied by agar well diffusion method. The diameter of zones of inhibition in each bacteria were measured using vernier caliper and reported in terms of millimeters. There was no observable zone of inhibition produced in any of the experimental plates of bacteria therefore showing the resistance of both S. aureus and E. coli to the flower extract components of Jasminum sambac.

CONCLUSION

After gathering and analyzing all the results and considering all factors that could have affected the conduct of the experimentation, the researchers came up with the following conclusion. The Sampaguita flower ethanolic extract was not effective in inhibiting the growth of the two organisms, S. aureus and E.coli . While Tetracycline and chloramphenicol both positively inhibited the growth of S aureus and E coli, the sampaguita flower extract, on the other hand, failed to produce any observable zone of inhibition in both

colonies of bacteria proving that the test organisms were resistant to any antimicrobial agents present in the extract.

RECOMMENDATIONS After drawing the conclusions of the study, the researchers made suggestions for future studies related to the said topic. Future studies regarding sampaguita extract as antimicrobial should be made to include other bacteria that pose threat to the health of many Filipinos. Also, to determine the ideal concentration of the extract against virulent bacteria, more dilutions and methods of extraction should be done and tested. This will confirm if active components were indeed present in the extract but only in very small amounts. The researchers also thought that to explore the possibility of producing an effective antibiotic from sampaguita extract, an accurate phytochemical analysis should be done to support previous experimental results and results from other studies. This will directly identify the active antibiotic component of the extract.

Since the researchers only focused on the flower extract, it is hereby recommended that comparative studies be done on extracts coming from different parts of the Jasminum sambac plant.

Finally, to contribute to the thrust of searching for other sources of medicine, the researchers recommend that studies be made on possible antimicrobial property of other plants belonging to the same family of sampaguita (Jasmine). Another study be made comparing the effectiveness of the extract collected through different methods of extraction.