Professional Documents
Culture Documents
Slant Stab Broth
Slant Stab Broth
pg. 1
RECIPES
Nutrient Broth (100mL): 1. Into a 250mL beaker place 0.3g beef extract, 0.5g peptone, 50mL distilled water 2. Gently heat (stir) until dissolved (will be colored). 3. Bring the volume to 100mL with distilled water. 4. Adjust the pH to 7.0 using 0.1N NaOH or ).1M HCl as needed. Nutrient Agar: Prepare exactly like nutrient broth BUT in step 1 add 1.5g of Agar. Marine Agar (100mL): 1. Into a 250mL beaker place 0.5g peptone, 0.1g yeast extract, 0.01g FePO4, 1.5g Agar. 2. Bring the volume to 100mL with aged seawater. 3. Gently heat, (stir) until dissolved. 4. Adjust the pH to 7.5 - 7.8.
pg. 2