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Lab Safety 3
Lab Safety 3
Christos Tsatsanis
http://molmedgp.med.uoc.gr
It's a Partnership
A safe and healthful research environment requires
that you, along with each of your colleagues,
participate actively in your institution's laboratory
safety program. It is important that you know and
follow the health, safety, and environmental
practices of your institution.
The safety program of your institution provides
essential services to help you practice safe science.
These include:
Danger Signs
Compressed gas Prohibition signs
signs
Emergency response
Eπείγοντα 2509
(ημέρες Γενικής Εφημερίας)
Παθολογική Κλινική 2299 (όλες τις ώρες)
Spill kits.
Proper handling.
Storage.
Emergency response.
Dangerous for the
Corrosive Flammable
environment
Face shields
Wear a face shield when
you are handling a very
large volume of a
hazardous chemical, or
when you need to protect
your face and your eyes.
For example, wear a face
shield when you are
removing a closed
container from liquid
nitrogen or working at a
transilluminator.
Gloves
Wearing gloves is a simple
and effective way to protect
yourself from chemical
contact, but the gloves must
be resistant to the specific
chemical with which you are
working.
No glove material is
impermeable to all chemicals,
therefore, the most effective
practice in using protective
gloves is to change them
frequently and whenever they
are contaminated.
Working with chemicals
Phenol-chloroform extraction is routinely
performed in most modern biology laboratories.
Like any experiment, you should know all the
chemicals in your protocol. Although substitute
protocols that do not require phenol are available
and used in many labs, extraction with phenol-
chloroform remains the traditional approach for
purifying nucleic acids. Continued use of this
procedure accounts for the fact that phenol is the
most frequently reported chemical exposure in
laboratories.
http://www.hhmi.org/about/labsafe/index.html
http://web.princeton.edu/sites/ehs/labsafetymanual/index.html
http://www.ehs.umass.edu/lhs.html
ETHIDIUM BROMIDE
You should know the types and energies of emitted radiations, the
potential for external exposure, the half-life, the annual limit on
intake (ALI), and how to detect contamination for the radionuclides
you use.