1

ur Plant Tissue Culture Tested basal salts and media have been formulated according to the references cited in their respective product listings. These basal salts and media are then manufactured and packaged in environmentally controlled facilities. Our biological testing ensures that each lot produces culture growth consistent with prior lots. On the following pages are many popular basal salt and media formulas followed by Murashige and Skoog-based formulations. Vitamin listings and formulations complete this section. Please contact us with inquiries about larger package sizes or custom formulations. Instructions for basal salt, media, and vitamin preparation

are provided in the Technical Information Section of this catalog and on our web site: www.phytotechlab.com.

asal Salt vs Medium... our definition

A Basal Salt Mixture contains macro- and micronutrients, but no vitamins, carbohydrates, or growth regulators. A Basal Medium contains the macro- and micronutrients, but is incomplete as it typically lacks an organic component: vitamins, carbohydrates, or growth regulators. A Medium typically is complete and requires no additional components (except gelling agent, if desired). A Modified Basal Salt, Basal Medium, or Medium indicates that one or more of the components varies in concentration or form from that originally published.

Plant Tissue Culture Basal Salts and Media

Product Number
A267

Product Description
ANDERSON BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Anderson (1978, 1980). Plant Tissue Culture Tested BANANA AGS BASAL MEDIUM Contains the macro- and micronutrients, vitamins, and plant growth regulators required to culture bananas. Plant Tissue Culture Tested Musa (Banana) Medium: See MS-Based Plant Specific Medium Section CAPE SUNDEW/ VENUS FLY TRAP MULTIPLICATION BASAL MEDIUM Contains the macro- and micronutrients, vitamins, and plant growth regulators required for the multiplication of carnivorous plants. Plant Tissue Culture Tested CAPE SUNDEW/ VENUS FLY TRAP PRETRANSPLANT BASAL MEDIUM Contains the macro- and micronutrients, vitamins, and plant growth regulators required for the growth of carnivorous plants. Plant Tissue Culture Tested CARROT CALLUS INITIATION BASAL MEDIUM Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate carrot callus from pith tissue. Plant Tissue Culture Tested CARROT SHOOT DEVELOPMENT BASAL MEDIUM Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate shoots from carrot callus. Plant Tissue Culture Tested CHEE & POOL C2d VITIS BASAL MEDIUM Contains the macro- and micronutrients as described by Chee & Pool (1987). Plant Tissue Culture Tested CHU N6 BASAL MEDIUM w/ VITAMINS Contains the macro- and micronutrients, and vitamins as described by Chu (1975). Plant Tissue Culture Tested CHU N6 BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Chu (1975). Plant Tissue Culture Tested CHU N6 VITAMIN SOLUTION (1000x) See Vitamin Section (following the MS-based media section) DCR BASAL SALT MIXTURE Contains macro- and micronutrients as described by Gupta & Durzan (1985). Plant Tissue Culture Tested

Product Notes
Storage Temp Soluble In Storage Temp Soluble In 2-6 C Water 2-6 C Water

Package Size
1L 10 L 50 L 1L 10 L 50 L

B144

M462 C206

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

C216

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

C212

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

C222

Storage Temp Soluble In

2-6 C Water

C287

Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water 2-6 C Water

C167

C416

C149 D146

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

2
Cape Sundew/ Venus Fly Trap Pretransplant Basal Medium Cape Sundew/Venus Fly Trap Multip. Basal Medium Anderson Basal Salt Mixture Carrot Callus Initiation Basal Medium Banana AGS Basal Medium Carrot Shoot Development Basal Medium Chu N6 Basal Salt Mixture DCR Basal Salt Mixture D146 400 463 6.2 492.3 0.025 0.025 74.5 36.7 55.7 180.7 16.9 0.25 181 16.9 0.25 55.7 180.7 16.9 0.25 90.5 8.45 0.125 0.025 0.025 0.025 0.025 74.5 18.35 27.8 122.09 10 0.25 27.8 122.09 10 0.25 27.8 180.6 0.845 0.25 27.85 90.37 3.3 27.85 90.37 3.3 27.8 180.7 22.3 0.25 0.025 0.0125 0.0125 0.025 0.025 37.26 0.025 0.025 37.26 0.025 0.025 37.3 37.25 37.25 1.6 125.33 463 1.6 125.33 6.2 64.14 386.31 0.025 0.25 37.3 0.83 340 170 8.6 1.64 4.0 rev 11/2005 Chu N6 Basal Medium w/ Vitamins C167 0.8 1900 170 2830 400 Chee & Pool C2d Vitis Basal Medium C287 1650 134 6.2 332.2 6.2 333 6.2 332.2 3.1 166.5 3 113.24 134 3 113.24

All components expressed in mg/L

COMPONENT Aluminum Chloride6H2O Ammonium Nitrate Ammonium Phosphate, Monobasic Ammonium Sulfate Boric Acid Calcium Chloride, Anhydrous Calcium Nitrate Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Chloride Ferric Sodium EDTA Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese Chloride4H2O Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Molybdenum Trioxide Nickel Chloride6H2O Nickel Sulfate6H2O Potassium Chloride Potassium Iodide Potassium Nitrate Potassium Phosphate, Dibasic Potassium Phosphate, Monobasic Potassium Sulfate Sodium Nitrate Sodium Phosphate Monobasic Sodium Sulfate Zinc Nitrate6H2O Zinc Sulfate7H2O Adenine Hemisulfate Glycine (Free Base) 2,4-Dichlorophenoxyacetic Acid 6-,-Dimethylallylaminopurine (2iP) Indole-3-acetic Acid Kinetin L(-)Malic Acid myo-Inositol Nicotinic Acid (Free Acid) PyridoxineHCI ThiamineHCI Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

A267 400

B144 1650

C206 400

C216 825

C212

C222

C416

0.3 480

0.83 1900 170 480

0.415 950 85

0.75 2500

0.75 2500

0.8 2830 400

330.6

295

380

150

150

8.6

8.6

8.6 80

4.3

8.6

1.5 2

1.5

1 10 1 0.2 100 100 50 100 1 1 0.4 1.89 3.7 4.71 5.7 0.4 2.12 3.5 0.2 2.20 4.3 10 3.21 3.0 100 1 1 10 3.21 4.0 10 1 1 1 4.49 4.0 0.5 0.5 1 3.99 4.0 3.98 4.1 1

Plant Tissue Culture Basal Salts and Media (continued)

Product Number
D190

Product Description
DKW BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan, et al (1987). DKW BASAL MEDIUM Contains 10 g/L Sucrose; without Vitamins Contains macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan, et al. (1987). Plant Tissue Culture Tested DKW BASAL MEDIUM Contains 30 g/L Sucrose; without vitamins Contains macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan, et al. (1987). Plant Tissue Culture Tested ERIKSSON VITAMIN SOLUTION (1000x) See Vitamin Section (following the MS-based media section) GAMBORG BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Gamborg, et al. (1968). Plant Tissue Culture Tested GAMBORG B-5 BASAL MEDIUM Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968). Plant Tissue Culture Tested GAMBORG (PRL-4-DM) LONG BASAL MEDIUM Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1966). Plant Tissue Culture Tested GAMBORG VITAMIN POWDER (1000x) See Vitamin Section (following the MS-based media section) GAMBORG VITAMIN SOLUTION (1000x) See Vitamin Section (following the MS-based media section) GRESSHOFF & DOY BASAL MEDIUM Contains the macro- and micronutrients as described by Gresshoff & Doy (1974). Plant Tissue Culture Tested HELLER BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Heller (1953). Plant Tissue Culture Tested HELLER/ WHITE MODIFIED BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Heller (1953) and White (1963). Without Molybdenum Trioxide Plant Tissue Culture Tested HOAGLAND MODIFIED BASAL SALT MIXTURE Contains Ferrous Sulfate Contains the macro- and micronutrients as described by Hoagland and Arnon (1938). Plant Tissue Culture Tested HOSTA Media See MS-Based Plant-Specific Media (following this section)

Product Notes
Storage Temp Soluble In Storage Temp Soluble In 2-6 C Water 2-6 C Water

Package Size
1L 10 L 50 L 1L 10 L 50 L

D191

D189

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

E330 G768

Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water

1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

G398

G359

Storage Temp Soluble In

2-6 C Water

G249 G219 G371

Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water 2-6 C Water

1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

H393

H396

H353

Storage Temp Soluble In

2-6 C Water

K413

KAO & MICHAYLUK BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Kao & Michayluk (1975). Plant Tissue Culture Tested KAO & MICHAYLUK MODIFIED BASAL MEDIUM Contains the macro- and micronutrients, vitamins, and organic acids as described by Kao & Michayluk (1975). Plant Tissue Culture Tested KAO & MICHAYLUK VITAMIN SOLUTION (100x) See Vitamin Section (following the MS-based media section)

Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water

1L 10 L 50 L 1L 10 L 50 L

K427

K421

4
Hoagland Modified Basal Salt Mixture DKW Basal Medium w/ 30 g/L Sucrose Kao & Michayluk Basal Salt Mixture DKW Basal Medium w/ 10 g/L Sucrose Gamborg (PRL-4-DM) Long Basal Medium

All components expressed in mg/L

COMPONENT Aluminum Chloride6H2O Ammonium Nitrate Ammonium Phosphate, Monobasic Ammonium Sulfate Boric Acid Calcium Chloride, Anhydrous Calcium Nitrate Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Chloride Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese Chloride4H2O Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Molybdenum Trioxide Nickel Chloride Nickel Sulfate6H2O Potassium Chloride Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Potassium Sulfate Sodium Nitrate Sodium Phosphate Monobasic Sodium Sulfate Zinc Nitrate6H2O Zinc Sulfate7H2O p-Aminobenzoic Acid L-Arginine (Free Base) L-Ascorbic Acid Asparagine D-Biotin D-Calcium Pantothenate Choline Chloride Citric Acid (Free Acid) Anhydrous Cyancobalamin, Vit B12 Folic Acid Fumaric Acid L-Glutamine Glycine (Free Base) L(-)-Maleic Acid L-Methionine myo-Inositol Nicotinamide Nicotinic Acid (Free Acid) L-Phenylalanine PyridoxineHCI Pyruvic Acid Riboflavin Sucrose ThiamineHCI L-Tryptophan Vitamin A Vitamin D3 Grams of powder to prepare 1 liter pH0.5 at RT

D190 1416

D191 1416

D189 1416

G768

G398

G359

G371 1000

H393 0.0543

H396 0.03

H353

K413 600

115.03 134 4.8 112.5 1367 0.25 45.4 33.8 361.49 33.5 0.39 4.8 112.5 1367 0.25 45.4 33.8 361.49 33.5 0.39 4.8 112.5 1367 0.025 0.25 45.4 33.8 361.49 33.5 0.39 0.025 37.26 27.8 122.09 10 0.25 0.025 0.025 37.26 27.8 122.09 10 0.25 0.25 0.25 186.0 139.0 122.09 132.0 0.25 3 113.24 134 3 113.24 200 3 113.24 241.2 0.025 0.025 37.25 1 27.85 17.099 1 0.025 0.016 0.03 0.005 0.005 0.005 300 0.75 2500 265 1559 265 1559 265 1559 600 150 17 17 17 2 2 3 0.2 40 0.4 40 0.00025 0.4 0.2 0.2 0.01 1 1 40 0.02 0.015 60 20 30.0 100 1 1 100 0.5 20 0.5 0.015 10,000 30,000 10 0.5 40 0.01 0.01 5.22 4.3 15.22 4.0 35.22 4.1 3.10 4.0 3.21 4.0 3.64 NS 2.71 3.9 1.64 4.9 1.04 4.7 1.63 4.5 3.65 4.0 3.9 4.4 1 1 0.1 1 20 0.2 10 0.1 100 1 4 40 0.4 40 2 0.3 1 1 0.22 2 2 0.02 150 90.0 108.75 16.5 200 0.75 2500 0.75 1000 65 0.8 1000 300 750 0.01 65 0.01 80 606.6 300 0.75 1900 170 300 0.75 1900 170 0.03 122.1 0.0758 25 351.63 0.01 2.5 240.76 1.81 27.85 146.55 10 0.25 27.85 146.55 10 0.25 0.03 0.03 0.08 3.35 0.3 1 56.7 300 656.4 0.025 0.025 37.26 0.025 0.025 37.26 1.24 2.86 3 453 3 453

NS = No Specification Established

rev 11/2005

Kao & Michayluk Modified Basal Medium

Heller Basal Salt Mixture

Gresshoff & Doy Basal Medium

DKW Basal Salt Mixture

Gamborg Basal Salt Mixture

Heller/ White Modified Basal Salt Mixture

Gamborg B-5 Basal Medium

K427 600

5
Product Number
L689

Product Description
LINSMAIER & SKOOG BASAL MEDIUM This medium may be sold as Murashige & Skoog Medium with Minimal Organics (MSMO) by some media suppliers. Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965). Plant Tissue Culture Tested LINSMAIER & SKOOG MODIFIED BASAL MEDIUM pH Adjusted and Buffered This medium may be sold as Murashige & Skoog Medium with Minimal Organics (MSMO) by some media suppliers. Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965). Plant Tissue Culture Tested LINSMAIER & SKOOG BASAL MEDIUM w/ 30 g/L SUCROSE & 7 g/L AGAR Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965). Plant Tissue Culture Tested LINSMAIER & SKOOG MODIFIED BASAL MEDIUM w/ 30 g/L SUCROSE & 7 g/L AGAR pH Adjusted and Buffered Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965). Plant Tissue Culture Tested LLOYD & McCOWN WOODY PLANT BASAL SALT MIXTURE Contains the WPM macro- and micronutrients as described by Lloyd & McCown (1981). Plant Tissue Culture Tested LLOYD & McCOWN WOODY PLANT BASAL MEDIUM w/ VITAMINS Contains the WPM macro- and micronutrients, and vitamins as described by Lloyd & McCown (1981). Plant Tissue Culture Tested LLOYD & McCOWN WOODY PLANT MICRONUTRIENT MIXTURE Contains the WPM micronutrients described by Lloyd & McCown (1981). Plant Tissue Culture Tested MG BASAL SALT MIXTURE Modified Murashige & Skoog/ Gamborg Basal Salt Mixture Plant Tissue Culture Tested MURASHIGE & SKOOG BASAL SALTS & MEDIA See Murashige & Skoog-Based Basal Salts & Media Section (following this section)

Product Notes
Storage Temp Soluble In 2-6 C Water

Package Size
1L 10 L 50 L

L477

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

L467

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

L452

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

L154

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

L449

Storage Temp Soluble In

2-6 C Water

L444

Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water

M419

N492

NB BASAL MEDIUM Modified Chu/ Gamborg Basal Medium Contains the macronutrients as described by Chu (1975) and the micronutrients as described by Gamborg, et al. (1968). Plant Tissue Culture Tested NITSCH & NITSCH BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Nitsch & Nitsch (1969). Plant Tissue Culture Tested NITSCH & NITSCH BASAL MEDIUM w/ VITAMINS Contains the macro- and micronutrients, and vitamins as described by Nitsch & Nitsch (1969). Plant Tissue Culture Tested NITSCH & NITSCH VITAMIN POWDER (1000x) See Vitamin Section (following the MS-based media section) NITSCH & NITSCH VITAMIN SOLUTION (1000x) See Vitamin Section (following the MS-based media section)

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

N613

Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water

1L 10 L 50 L 1L 10 L 50 L

N616

N608 N603

6
Lloyd & McCown Woody Plant Micronutrient Mixture Nitsch & Nitsch Basal Salt Mixture N613 720 33.5 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 0.83 1900 170 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 100 0.83 1900 170 0.83 1900 170 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 100 0.83 1900 170 170 990 170 990 437.8 32.6 8.6 8.6 8.6 7000 8.6 7000 0.05 0.5 2 1000 100 100 100 1000 100 100 0.5 0.5 30,000 0.4 4.43 NS 0.4 5.53 0.4 41.43 6.2 30,000 0.4 42.53 NS 2.30 NS 1 2.41 NS 0.53 NS 1.88 4.3 10 4.10 4.0 2.10 3.8 0.5 2.21 NS rev 11/2005 100 1 1 100 5 0.5 2 8.6 8.6 8.6 2.7 2 10 10 170 0.4 1100 42.5 0.75 2830 400 950 68 950 68 0.25 37.3 27.85 180.7 22.3 0.25 0.25 37.3 27.85 180.7 22.3 0.25 0.25 37.3 27.85 180.7 22.3 0.25 6.2 72.5 386 6.2 72.5 386 0.0125 0.0125 18.64 13.9 75.7 6.7 0.125 0.025 0.025 37.26 27.8 90.37 10 0.25 0.025 37.26 27.8 90.372 18.9 0.25 0.025 37.26 27.8 90.372 18.9 0.25 6.2 72.5 2.3 111.36 463 3 125.33 10 166 10 166 Linsmaier & Skoog Basal Medium Lloyd & McCown Woody Plant Basal Salt Mixture Lloyd & McCown Woody Plant Basal Medium Linsmaier & Skoog Modified Basal Medium, Buffered & pH Adjusted Linsmaier & Skoog Modified Basal Medium (MSMO) MG Basal Salt Mixture (Modified MS/Gamborg Basal Salt Mixture) Nitsch & Nitsch Basal Medium N616 720 Linsmaier & Skoog Basal Medium (MSMO) NB Basal Medium N492

All components expressed in mg/L

COMPONENT Ammonium Nitrate Ammonium Sulfate Boric Acid Calcium Chloride, Anhydrous Calcium Nitrate Cobalt Chloride6H2O Cupric Sulfate5H2O
Na2EDTA2H2O

L689 1650

L477 1650

L467 1650

L452 1650

L154 400

L449 400

L444

M419

Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Potassium Hydroxide Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Potassium Sulfate Sodium Nitrate Sodium Phosphate Monobasic Zinc Sulfate7H2O Agar D-Biotin Folic Acid Glycine (Free Base) MES (Free Acid) myo-Inositol Nicotinic Acid (Free Acid) PyridoxineHCI Sucrose ThiamineHCI Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

Plant Tissue Culture Basal Salts and Media (continued)

Product Number
N479

Product Description
NLN BASAL MEDIUM Contains the macro- and micronutrients, and organic constituents as described by Lichter (1982); without sucrose. Plant Tissue Culture Tested PARKER THOMPSON BASIC C FERN BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Hickok, et al. (1995). Formulated for the growth of Ceratopteris richardii. Plant Tissue Culture Tested QUOIRIN & LEPOIVRE BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Quoirin & Lepoivre (1977) and Quoirin, et al. (1977). Plant Tissue Culture Tested ROSE MODIFIED INITIATION BASAL MEDIUM Stage I Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested ROSE MODIFIED MULTIPLICATION BASAL MEDIUM Stage II Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested ROSE MODIFIED ROOTING BASAL MEDIUM Stage III Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested SCHENK & HILDEBRANDT BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Schenk and Hildebrandt (1972). Plant Tissue Culture Tested SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM Contains 10 g/L Sucrose; Without Vitamins Plant Tissue Culture Tested SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM Contains 10 g/L sucrose, 12x vitamins, 12x micro- and 12x macronutrients. Plant Tissue Culture Tested SCHENK & HILDEBRANDT MODIFIED BASAL SALT MIXTURE Without Calcium Plant Tissue Culture Tested SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM Contains vitamins and 10g/L sucrose. Plant Tissue Culture Tested SCHENK & HILDEBRANDT VITAMIN POWDER (100x) See Vitamin Section (following the MS-based media section)

Product Notes
Storage Temp Soluble In 2-6 C Water

Package Size
1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

P713

Storage Temp Soluble In

2-6 C Water

Q673

Storage Temp Soluble In

2-6 C Water

R756

Storage Temp Soluble In

2-6 C Water

R757

Storage Temp Soluble In

2-6 C Water

R758

Storage Temp Soluble In

2-6 C Water

S816

Storage Temp Soluble In

2-6 C Water

S811

Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water

S808

S806

S813

S826

8
Parker-Thompson Basic C Fern Basal Salt Mixture Quoirin & Lepoivre Basal Salt Mixture Rose Modified Initiation Basal Medium Schenk & Hildebrandt Modified Basal Medium Schenk & Hildebrandt Modified Basal Medium Schenk & Hildebrandt Modified Basal Medium S813 300 5.0 151 0.1 0.2 20 15 195.4 10 0.1 1.0 2500 1.0 0.1 0.2 20 15 195.4 10 0.1 1.0 2500 1.0 1000 5.0 0.5 10000 5.0 3.05 4.3 14.21 4.3 rev 11/2005 Rose Modified Rooting Basal Medium Schenk & Hildebrandt Basal Salt Mixture Schenk & Hildebrandt Modified Basal Salt Mixture S806 300 5.0 NLN Basal Medium Rose Modified Multiplication Basal Medium R757 1650 6.2 333 0.025 0.025 36.7 181 16.9 0.25 0.83 1900 170 8.6 50 3.0 50

All components expressed in mg/L

COMPONENT Ammonium Molybdate Ammonium Nitrate Ammonium Phosphate, Monobasic Boric Acid Calcium Chloride, Anhydrous Calcium Nitrate Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Sodium EDTA Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Zinc Sulfate7H2O L-Ascorbic Acid 6-Benzylaminopurine (BA) D-Biotin Citric Acid (Free Acid) Anhydrous Folic Acid L-Glutamine Glutathion Glycine (Free Base) Indole-3-acetic Acid myo-Inositol -Naphthaleneacetic Acid Nicotinic Acid (Free Acid) PyridoxineHCI L-Serine Sucrose ThiamineHCI Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

N479

P713 0.037 125

Q673 400 6.2 833.77 0.025

R756 1650 6.2 333 0.025 0.025 36.7

R758 412.5

S816

S811

S808

300 10 347 0.025 0.025 36.7 27.8 61 18.95 0.25 125 125 10 500 0.52 58.565 0.25 27.8 175.79 0.76 0.25 0.08 1800 270 8.6 181 16.9 0.25 0.83 1900 170 8.6 50 2.0 0.05 50 0.5 800 30 2.0 100 5.0 0.5 100 2.0 0.30 100 0.5 0.5 2.0 0.30 100 0.5 0.5 100 0.03 0.5 0.5 2.0 45.25 4.225 0.063 0.208 475 42.5 2.15 1.0 0.37 37.3 1.86 19.628 1.55 83.25 0.006 0.006 9.175 15 195.4 10 0.1 1.0 2500 5.0 151 0.1 0.2 20

300 5.0 151 0.1 0.2 20 15 195.4 10 0.1 1.0 2500 1.0

150 2.5 75.5 0.05 0.1 10 7.5 97.7 5 0.05 0.5 1250 0.5

0.025 37.3

500 2.5 0.25 10000 10000 2.5 3.20 4.2 13.20 4.3 12.10 4.5

0.5 1.93 4.5 0.77 3.8 3.56 3.9

0.4 4.51 3.5

0.4 4.51 3.5

0.4 1.18 5.0

Plant Tissue Culture Basal Salts and Media (continued)

Product Number
T853 Plant Tissue Culture Tested T868 TM4G BASAL MEDIUM Plant Tissue Culture Tested T856 TOBACCO MODIFIED CALLUS INITIATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested TOBACCO MODIFIED SHOOT MULTIPLICATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested TOBACCO MODIFIED SHOOT & ROOT BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested TOBACCO MODIFIED ROOT INITIATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA Plant Tissue Culture Tested WESTVACO WV3 BASAL MEDIUM Contains the macro- and micronutrients, and vitamins as described by Coke (1996b). Plant Tissue Culture Tested WESTVACO WV5 BASAL MEDIUM Contains the macro- and micronutrients, and vitamins as described by Coke (1996a). Plant Tissue Culture Tested WHITE BASAL SALT MIXTURE Contains the macro- and micronutrients as described by White (1963). Plant Tissue Culture Tested

Product Description
TM4G BASAL SALT MIXTURE

Product Notes
Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water

Package Size
1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

T864

T867

T861

W863

W865

W898

Murashige & Skoog Basal Salts and Media following this section.

10
Tobacco Modified Shoot & Root Basal Medium TM4G Basal Salt Mixture White Basal Salt Mixture W898 1.5 208.5 0.025 0.025 18.65 0.025 0.025 18.65 36.7 13.9 122.12 16.9 0.25 0.83 1900 13.9 122.12 16.9 0.25 0.83 1900 181 16.9 0.25 0.83 1900 170 181 16.9 0.25 0.83 1900 170 181 16.9 0.25 0.83 1900 170 181 16.9 0.25 0.83 1900 170 903.79 15.16 0.25 656.79 0.83 910.06 270 903.79 15.16 0.25 718.67 0.83 1084.06 270 16.5 200 9.2 9.2 0.05 1000 0.5 2.5 2.0 2.0 0.2 100 5.0 0.5 0.5 2.88 NS 2.99 NS 100 0.5 0.5 0.4 5.41 5.5 1.0 100 0.5 0.5 0.4 5.41 4.3 2.0 2.0 0.03 1.0 100 0.5 0.5 0.4 5.41 NS 100 0.5 0.5 0.4 5.41 5.0 NS 0.4 0.4 5.22 NS 0.934 4.7 rev 11/2005 1000 1000 2.0 3.0 1000 1000 1000 8.6 8.6 8.6 8.6 8.6 8.6 3.0 65.0 0.75 80.0 351.62 5.31 0.001 36.7 36.7 36.7 36.7 36.71 2.5 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.25 0.025 0.25 0.001 Tobacco Modified Callus Initiation Basal Medium Tobacco Modified Shoot Multiplication Basal Medium Tobacco Modified Root Initiation Basal Medium Westvaco WV3 Basal Medium Westvaco WV5 Basal Medium W865 700 31.0 452.88 TM4G Basal Medium T868 320 230 130 6.2 113.25 6.2 333 6.2 333 6.2 333 6.2 333 31.0 452.88

All components expressed in mg/L

COMPONENT Ammonium Nitrate Ammonium Phosphate, Monobasic Ammonium Sulfate Boric Acid Calcium Chloride, Anhydrous Calcium Nitrate Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Sodium EDTA Ferric Sulfate Ferrous Sulfate Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdenum Trioxide Molybdic Acid (Sodium Salt)2H2O Potassium Chloride Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Sodium Phosphate Monobasic Sodium Sulfate Zinc Sulfate7H2O D-Biotin Casein, Enzymatic Hydrolysate Folic Acid Glycine (Free Base) Indole-3-acetic Acid Kinetin myo-Inositol Nicotinic Acid (Free Acid) PyridoxineHCI ThiamineHCI Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

T853 320 230 130 6.2 113.25

T856 1650

T864 1650

T867 1650

T861 1650

W863

11

Murashige & Skoog-Based Basal Salts

Product Number
M524

Product Description
MURASHIGE & SKOOG BASAL SALT MIXTURE Contains the macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested See Product No. M519 for Murashige & Skoog Basal Medium w/ Vitamins MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Contains FeNaEDTA Plant Tissue Culture Tested MURASHIGE & SKOOG MACRONUTRIENT SALT BASE Contains the macronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MACRONUTRIENT STOCK SOLUTION (10x) Contains the macronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MICRONUTRIENT SALT BASE Contains the micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MICRONUTRIENT STOCK SOLUTION (10x) Contains the micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Contains 12x macro- and 12x micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Contains 12x Ammonium Nitrate and 12x Potassium Nitrate, with the remaining macroand micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Contains 12x Ammonium Nitrate, 12x Potassium Nitrate, and 12x Calcium Chloride, with the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Without Ammonium Nitrate. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Without Nitrogen. Contains the macro- and micronutrients as described by Murashige & Skoog (1962), modified by eliminating NH4NO3 and KNO3. Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Without Nitrogen, Phosphorous, and Potassium Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested

Product Notes
Storage Temp Soluble In 2-6 C Water

Package Size
1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

M499

Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water

M502

M654

M554

M529

M153

M561

Storage Temp Soluble In

2-6 C Water

M290

Storage Temp Soluble In

2-6 C Water

M571

Storage Temp Soluble In

2-6 C Water

M531

Storage Temp Soluble In

2-6 C Water

M407

Storage Temp Soluble In

2-6 C Water

12
MS Macronutrient Salt Base MS Micronutrient Salt Base Murashige & Skoog (MS) Basal Salt Mixture MS Macronutrient Stock Solution (10x) MS Modified Basal Salt Mixture w/ FeNaEDTA MS Modified Basal Salt Mixture (1/2x Micros & Macros) MS Modified Basal Salt Mixture (1/2x Nitrates) MS Modified Basal Salt Mixture MS Modified Basal Salt Mixture (No NH4NO3) MS Modified Basal Salt Mixture (No Nitrogen) MS Modified Basal Salt Mixture (No N, P, or K) MS Micronutrient Stock Solution (10x)

All components expressed in mg/L

COMPONENT
Ammonium Nitrate Boric Acid Calcium Chloride, Anhydrous Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Sodium EDTA Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Zinc Sulfate7H2O Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

M524
1650 6.2 332.2 0.025 0.025 37.26

M499
1650 6.2 333 0.025 0.025

M502
1650

M654
16500

M554
6.2

M529
62

M153
825 3.1 166.1

M561
825 6.2 332.2 0.025 0.025 37.26

M290
825 6.2 166.1 0.025 0.025 37.26

M571
6.2 332.2 0.025 0.025 37.26

M531
6.2 332.2 0.025 0.025 37.26

M407
6.2 332.2 0.025 0.025 37.26

332.2

3322 0.025 0.025 37.26 0.25 0.25 373

0.0125 0.0125 18.63

36.7 27.8 180.7 16.9 0.25 0.83 1900 170 8.6 4.33 3.9 181 16.9 0.25 0.83 1900 170 8.6 4.30 NS 4.23 NS N/A 4.3 1900 170 19000 1700 8.6 0.10 4.3 86 NA 3.2 180.7 1810 16.9 0.25 0.83 169 2.5 8.30 27.8 278 13.9 90.35 8.45 0.125 0.415 950 85 4.3 2.17 NS 27.8 180.7 16.9 0.25 0.83 950 170 8.6 2.56 4.3 27.8 180.7 16.9 0.25 0.83 950 170 8.6 2.39 NS 27.8 180.7 16.9 0.25 0.83 1900 170 8.6 2.68 NS 170 8.6 0.78 4.3 8.6 0.61 4.3 27.8 180.7 16.9 0.25 0.83 27.8 180.7 16.9 0.25 0.83

Rev. 11/2005

13

Murashige & Skoog-Based Media

Product Number
M519

Product Description
MURASHIGE & SKOOG BASAL MEDIUM w/ VITAMINS With the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962). Plant Tissue Culture Tested Contains the basal salts of Product No. M524 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM Without Potassium Phosphate; contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients, and modified vitamins as described by Murashige & Skoog (1962). Also contains (mg/L): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, and 1000 Casein Hydrolysate. Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL MEDIUM w/ GAMBORG VITAMINS With the macro- and micronutrients as described by Murashige & Skoog (1962), and vitamins as described by Gamborg, et al. (1966). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED MEDIUM Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modified with the addition of (mg/L): 1.0 BA and 0.1 NAA Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL MEDIUM This medium was formerly listed as M506. This medium may be sold as Murashige Begonia Multiplication Medium by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modified with the addition of (mg/L): 10 2iP and 30 Adenine Hemisulfate. Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED MEDIUM Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modified with the addition of (mg/L): 30 2iP and 80 Adenine Hemisulfate, and 0.3 IAA Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BASAL MEDIUM Without Nicotinic Acid, PyridoxineHCl, and Glycine Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modified with the addition of (mg/L) 0.4 ThiamineHCl, 100 myo-Inositol, and 80 Adenine Hemisulfate. Plant Tissue Culture Tested MURASHIGE MEDIUM WITH MINIMAL ORGANICS (MSMO) See L689 & L477 Linsmaier & Skoog Basal Medium

Product Notes
Storage Temp Soluble In 2-6 C Water

Package Size
1L 10 L 50 L

M541

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

M404

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

M401

Storage Temp Soluble In

2-6 C Water

M701

Storage Temp Soluble In

2-6 C Water

M702

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L 1L 10 L 50 L

M535

Storage Temp Soluble In

2-6 C Water

M536

MURASHIGE MODIFIED MULTIPLICATION BASAL MEDIUM Contains the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962); modified with the addition of (mg/L): 2.0 2iP, 80 Adenine Hemisulfate, and 170 Sodium Phosphate. Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED MULTIPLICATION MEDIUM This medium may be sold as Murashige & Skoog Shoot Multiplication Medium C by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modified with the addition of (mg/L): 1.0 Kinetin, 80 Adenine Hemisulfate, and 148 Sodium Phosphate Monobasic. Plant Tissue Culture Tested MURASHIGE MODIFIED MULTIPLICATION BASAL MEDIUM This medium is sold as Murashige Shoot Tip Rooting Medium by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and vitamins as described by Linsmaier & Skoog (1965); modified with the addition of (mg/L): 0.3 IAA, 1.0 Kinetin, and FeNaEDTA. Plant Tissue Culture Tested MURASHIGE MODIFIED SHOOT MULTIPLICATION BASAL MEDIUM This medium is sold as MS Shoot Multiplication Medium A by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and vitamins as described by Linsmaier & Skoog (1965); modified with the addition of (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30 2iP, and 0.3 IAA. Plant Tissue Culture Tested MURASHIGE & SKOOG VITAMIN POWDERS & SOLUTIONS See Vitamin Section (following this section)

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

M555

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

M527

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

M491

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

14
Murashige Modified Multiplication Basal Medium w/ 2iP MS Modified Medium w/ 2iP & IAA Murashige Modified Multiplication Basal Medium w/ Kinetin & IAA M527 1650 6.2 333 0.025 0.025 36.7 27.8 181 16.9 0.25 0.83 1900 170 8.6 30 1 1000 2 2 2 10 1 30 0.3 1 100 0.5 0.5 0.1 4.43 3.9 100 5 1 0.5 5.69 4.0 100 1 1 10 4.44 4.0 100 0.1 0.5 0.5 30000 0.4 34.44 4.0 0.4 4.44 3.9 30000 0.4 34.69 3.8 0.4 4.51 4.0 0.4 4.68 3.5 0.5 0.5 30000 0.4 34.66 4.4 0.4 4.41 0.4 4.68 100 100 100 100 100 0.1 0.3 1 100 100 2.0 30 0.3 180.7 16.9 0.25 0.83 1900 170 148 8.6 8.6 8.6 80 8.6 80 27.8 180.7 16.9 0.25 0.83 1900 170 27.8 180.7 16.9 0.25 0.83 1900 170 170 8.6 80 27.8 180.7 16.9 0.25 0.83 1900 170 148 8.6 80 8.6 181 16.9 0.25 0.83 1900 170 181 16.9 0.25 0.83 1900 170 170 8.6 80 Murashige Modified Shoot Multiplication Basal Medium M491 1650 6.2 333 0.025 0.025 36.7 MS Modified Medium w/ BA & NAA MS Modified Basal Medium (w/out KH2PO4) MS Modified Basal Medium w/ Gamborg Vitamins MS Modified Basal Medium w/ 2iP MS Modified Basal Medium MS Modified Multiplication Medium w/ Kinetin M555 1650 6.2 332.2 0.025 0.025 37.26 Murashige & Skoog Basal Medium M519 1650 6.2 332.2 0.025 0.025 37.26 36.7 27.8 180.7 16.9 0.25 0.83 1900 170 300 8.6 8.6 150 180.7 16.9 0.25 0.83 1900 27.8 180.7 16.9 0.25 0.83 1900 170 27.8 180.7 16.9 0.25 0.83 1900 170

All components expressed in mg/L

COMPONENT Ammonium Nitrate Boric Acid Calcium Chloride, Anhydrous Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Sodium EDTA Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Sodium Phosphate Monobasic Zinc Sulfate7H2O Adenine Hemisulfate 6-Benzylaminopurine (BA) Casein, Enzymatic Hydrolysate Glycine (Free Base) 6-,-Dimethylallylaminopurine (2iP) Indole-3-acetic Acid Indole-3-butyric Acid Kinetin myo-Inositol -Naphthaleneacetic Acid Nicotinic Acid (Free Acid) PyridoxineHCI Sucrose ThiamineHCI Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

M541 1650 6.2 332.2 0.025 0.025

M404 1650 6.2 332.2 0.025 0.025 37.26

M401 1650 6.2 332.2 0.025 0.025 37.26

M701 1650 6.2 333 0.025 0.025 36.7

M702 1650 6.2 332.2 0.025 0.025 37.26

M535 1650 6.2 332.2 0.025 0.025 37.26

M536 1650 6.2 332.2 0.025 0.025 37.26

NS NS Rev 11/2005

15 MS-Based Plant-Specific Media

Product Number
M517

Product Description
MURASHIGE MODIFIED AFRICAN VIOLET/ GLOXINIA MULTIPLICATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE MODIFIED AFRICAN VIOLET/ GLOXINIA PRETRANSPLANT BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED MEDIUM Arabidopsis Culture Medium Modified with the addition of (mg/L): 2.0 2,4-D, and 0.05 Kinetin Plant Tissue Culture Tested MURASHIGE BEGONIA MULTIPLICATION MEDIUM See Product No. M701 Murashige & Skoog Modified Medium (same formulation) MURASHIGE MODIFIED FERN MULTIPLICATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE MODIFIED GERBERA MULTIPLICATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE MODIFIED GERBERA PRETRANSPLANT BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested HOSTA INITIATION/ MULTIPLICATION MEDIUM Stage I/II Medium Modified Murashige & Skoog (1962) with (mg/L): 2.0 BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, and 0.5 NAA Plant Tissue Culture Tested HOSTA MULTIPLICATION MEDIUM Stage II Medium Modified Murashige & Skoog (1962) with (mg/L): 0.1 BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, and 0.5 NAA Plant Tissue Culture Tested HOSTA ROOTING MEDIUM Stage III Medium Modified Murashige & Skoog (1962) with (mg/L): 0.1 BA, 500 Casein Hydrolysate, and 0.5 NAA Plant Tissue Culture Tested

Product Notes
Storage Temp Soluble In 2-6 C Water

Package Size
1L 10 L 50 L

M518

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

M550

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

M506 M508

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

M509

Storage Temp Soluble In

2-6 C Water

M510

Storage Temp Soluble In

2-6 C Water

H435

Storage Temp Soluble In

2-6 C Water

H436

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

H437

Storage Temp Soluble In

2-6 C Water

1L 10 L 50 L

16
Murashige Modified African Violet / Gloxinia Pretransplant Basal Medium Murashige Modified African Violet/ Gloxinia Multiplication Basal Medium Murashige & Skoog Basal Medium (Published Formula Reference) M519 1650 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 0.83 1900 170 8.6 2.0 100 0.5 0.5 30000 0.4 4.66 4.5 30 100 4.66 4.0 4.4 4.8 24.44 4.0 4.72 NS 30 100 4.64 5.9 43.40 4.3 43.39 4.3 43.23 4.3 4.43 3.9 Rev 11/2005 0.4 30000 0.4 30000 0.4 0.1 Hosta Initiation/ Multiplication Medium Murashige Modified Gerbera Multiplication Basal Medium Murashige Modified Gerbera Pretransplant Basal Medium Hosta Multiplication Medium Murashige Modified Fern Multiplication Basal Medium

All components expressed in mg/L

COMPONENT Ammonium Nitrate Boric Acid Calcium Chloride, Anhydrous Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Sodium EDTA Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Sodium Phosphate Monobasic Zinc Sulfate7H2O Adenine Hemisulfate Agar 6-Benzylaminopurine (BA) Casein, Enzymatic Hydrolysate Glycine (Free Base) 2,4-Dichlorophenoxyacetic Acid Indole-3-acetic Acid Indole-3-butyric Acid Kinetin MES (Free Acid) myo-Inositol a-Naphthaleneacetic Acid Nicotinic Acid (Free Acid) Peptone from Meat PyridoxineHCI Sucrose ThiamineHCI L-Tyrosine Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

M517 1650 6.2 333 0.025 0.025 36.7 181 16.9 0.25 0.83 1900 170 170 8.6 80

M518 1650 6.2 333 0.025 0.025 36.7

M550 1650 6.2 332.2 0.025 0.025 37.26

M508 1650 6.2 333 0.025 0.025 36.7

M509 1650 6.2 333 0.025 0.025 36.7 181 16.9 0.25 0.83 1900 170 85 8.6 80

M510 1650 6.2 333 0.025 0.025 36.7

H435 1650 6.2 332.2 0.025 0.025 37.26 27.8

H436 1650 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 0.83 1900 300 170 8.6 160 8000 0.1 500 2.0

H437 1650 6.2 332.2 0.025 0.025 37.26 27.8 180.7 16.9 0.25 0.83 1900 300 170 8.6 8000 0.1 500

27.8 181 16.9 0.25 0.83 1900 170 8.6 180.7 16.9 0.25 0.83 1900 170 8.6 181 16.9 0.25 0.83 1900 170 255 8.6 181 16.9 0.25 0.83 1900 170 85 8.6

180.7 16.9 0.25 0.83 1900 300 170 8.6 160 8000 2.0 500 2.0

2.0 2.0 2.0 100 100 1.0 0.05 100 1.0 1.0 20000 0.4 0.4 10 2.0 100 0.1 10 1.0 10 1.0 0.5 10 100 100 100 0.5 100 0.5 100 0.5 10

Hosta Rooting Medium

MS Modified Medium (Arabidopsis)

17 MS-Based Plant-Specific Media (continued)

Product Number
M511

Product Description
MURASHIGE MODIFIED KALANCHOE MULTIPLICATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE MODIFIED KALANCHOE PRETRANSPLANT BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE MODIFIED LILY MULTIPLICATION BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MUSA (BANANA) MULTIPLICATION MEDIUM IITA Formulation as described by Vuylsteke (1998). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED BC POTATO BASAL MEDIUM Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962). Plant Tissue Culture Tested Murashige Modified Kalanchoe Multplication Basal Medium Murashige Modified Kalanchoe Pretransplant Basal Medium Murashige Modified Lily Multiplication Basal Medium

Product Notes
Storage Temp Soluble In 2-6 C Water

Package Size
1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L 1L 10 L 50 L

M512

Storage Temp Soluble In

2-6 C Water

M513

Storage Temp Soluble In

2-6 C Water

M462

Storage Temp Soluble In Storage Temp Soluble In

2-6 C Water 2-6 C Water

M516

All components expressed in mg/L

COMPONENT Ammonium Nitrate Boric Acid Calcium Chloride, Anhydrous Cobalt Chloride6H2O Cupric Sulfate5H2O Na2EDTA2H2O Ferric Sodium EDTA Ferrous Sulfate7H2O Magnesium Sulfate, Anhydrous Manganese SulfateH2O Molybdic Acid (Sodium Salt)2H2O Potassium Iodide Potassium Nitrate Potassium Phosphate, Monobasic Zinc Sulfate7H2O L-Ascorbic Acid 6-Benzylaminopurine (BA) Gelrite D-Glucose Glycine (Free Base) 6-,-Dimethylallylaminopurine (2iP) Indole-3-acetic Acid Kinetin myo-Inositol -Naphthaleneacetic Acid Nicotinic Acid (Free Acid) PyridoxineHCI Sucrose ThiamineHCI Grams of powder to prepare 1 liter pH0.5 at RT NS = No Specification Established

M511 1650 6.2 333 0.025 0.025 36.7 181 16.9 0.25 0.83 1900 170 8.6

M512 1650 6.2 333 0.025 0.025 36.7 181 16.9 0.25 0.83 1900 170 8.6

M513 1650 6.2 333 0.025 0.025 36.7

M462 1650 6.2 332.2 0.025 0.025 37.25

27.85 181 16.9 0.25 0.83 1900 170 8.6 180.74 16.9 0.25 0.83 1900 170 8.6 20 4.5 2000 2.0 2.0 3.0 3.0 100 100 100 0.03 0.5 0.5 30,000 0.4 4.41 4.8 0.4 4.41 4.8 0.4 4.4 4.8 0.4 36.36 4.3 0.4 4.41 NS 0.5 0.5 0.175 0.04 100 2.0 181 16.9 0.25 0.83 1900 170 8.6

MS Modified BC Potato Basal Medium

M516 1650 6.2 333 0.025 0.025 36.7

Musa (Banana) Multiplication Medium

18

Vitamins
Product Number
C149

Product Description
CHU N6 VITAMIN SOLUTION (1000x) Contains the vitamins as described by Chu (1975). Plant Tissue Culture Tested ERIKSSON VITAMIN SOLUTION (1000x) Contains the vitamins as described by Eriksson (1965). Plant Tissue Culture Tested GAMBORG VITAMIN POWDER (1000x) Contains the vitamins as described by Gamborg, et al. (1968). Plant Tissue Culture Tested GAMBORG VITAMIN SOLUTION (1000x) Contains the vitamins as described by Gamborg, et al. (1968). Plant Tissue Culture Tested KAO & MICHAYLUK VITAMIN SOLUTION (100x) Contains the vitamins described by Kao & Michayluk (1975). Plant Tissue Culture Tested MURASHIGE & SKOOG VITAMIN POWDER (1000x) Contains the vitamins as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG VITAMIN SOLUTION (1000x) Contains the vitamins as described by Murashige & Skoog (1962). Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED VITAMIN POWDER (1000x) Murashige & Skoog (1962) vitamins modified with additional ThiamineHCl. Plant Tissue Culture Tested MURASHIGE & SKOOG MODIFIED VITAMIN SOLUTION (1000x) Murashige & Skoog (1962) vitamins modified with additional ThiamineHCl. Plant Tissue Culture Tested NITSCH & NITSCH VITAMIN POWDER (1000x) Contains the vitamins as described by Nitsch & Nitsch (1969). Plant Tissue Culture Tested NITSCH & NITSCH VITAMIN SOLUTION (1000x) Contains the vitamins as described by Nitsch & Nitsch (1969). Plant Tissue Culture Tested SCHENK & HILDEBRANDT VITAMIN POWDER (100x) Contains the vitamins as described by Schenk & Hildebrandt (1972). Plant Tissue Culture Tested
Gamborg Vitamin Powder (1000x) Gamborg Vitamin Solution (1000x) Kao & Michayluk Vitamin Solution (100x) Eriksson Vitamin Solution (1000x) Chu N6 Vitamin Solution (1000x) MS Vitamin Solution (1000x) MS Vitamin Powder (1000x) MS Modified Vitamin Powder (1000x)

Product Notes
Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water

Package Size
100 mL

E330

100 mL

G249

100 mL

G219

100 mL

K421

100 mL 500 mL 1L 100 mL 250 mL 100 mL

M533

M553

M547

100 mL 250 mL Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In Storage Temp Soluble In
MS Modified Vitamin Solution (1000x)

M557

2-6 C Water 2-6 C Water 2-6 C Water 2-6 C Water

Nitsch & Nitsch Vitamin Solution (1000x)

100 mL

N608

100 mL 250 mL 100 mL

N603

S826

100 mL 1L
Schenk & Hildebrandt Vitamin Powder (100x)

All components expressed in mg/L

COMPONENT p-Aminobenzoic Acid L-Ascorbic Acid D-Biotin D-Calcium Pantothenate Choline Chloride Cyancobalamin, Vit B12 Folic Acid Glycine (Free Base) myo-Inositol Nicotinamide Nicotinic Acid (Free Acid) PyridoxineHCl Riboflavin ThiamineHCI Vitamin A Grams of powder to prepare 1 L

C149

E330

G249

G219

K421 2.0 200 1.0 100 100 2.0 40.0.0

M533

M553

M547

M557

Nitsch & Nitsch Vitamin Powder (1000x)

N608

N603

S826

50

50

500 2000 2000 100000 500 500 100 N/A 2000 100000 500 500 1000 104 2000 100000 500 500 1000 N/A 2000 100000 5000 500 500 108.55 100000 500

500 2000 100000 5000 500 500 N/A 100000 500 50.0 500 101.05 Rev 11/2005

2000

2000 100000 100000 1000 1000 10000 N/A 100 20.0 100 1.0 N/A 10000 100

500 500 1000 N/A

500 500 500 N/A

1000 1000 10000 112

500 100 103.1