Professional Documents
Culture Documents
Poster Scanning Microwave Microscopy Cells
Poster Scanning Microwave Microscopy Cells
Poster Scanning Microwave Microscopy Cells
T.Monti1, T.Pietrangelo2, M.Farina1 1 Universita Politecnica delle Marche, Information Engineering Dept. (DII), Ancona (Italy)
2Universita
degli studi G.dAnnunzio, Basic and Applied Medical Sciences Dept. (BAMS), Chieti (Italy)
Abstract In this work, we describe the application of an in-house system performing simultaneously Scanning Tunneling Microscopy (STM) and wide-band Near Field Scanning Microwave Microscopy
(wide-band SMM) to a biological sample. Glycerinated rabbit myofibrils are deposited on a substrate of Indium Tin Oxide (ITO)-coated glass. By introducing the time-domain conversion of frequency domain data, we show that it is possible to achieve nanometric resolution.
Ipodermic needle
STM tipsupport
Sample
Preparation of myofibrils
A glycerinated skeletal muscle tissue of rabbit (R. PSOAS) is
The proper time instant allows to exclude secondary reflections, bidistilled water). We use IKA T10 BASIC homogenizer also coming from the rest of the system, delayed in time. The direct reflection from the sample gives a time-domain image workcenter. The solution, of it. Any microwave image is inevitably the convolution of local containing isolated myofibrils, is and non-local interactions: easy interpretation of TD data. [1] then deposited over an ITOcoated glass slice. [2]
I band
glycerinated myofibrils sample. By using our in-house near-field SMM and through the TD conversion of the frequency domain data, we obtained nanometric scale images of electromagnetic features of the cells. STM image SMM (time) The work is ongoing, also considering the interaction of the microwave field with subsurface object. The main limitation of the lack of the spatial resolution in buried detection (recall microwave tomography), [1] Farina M., et al., Disentangling Time in a Near-Field Approach to the Scanning Probe Microscopy, Nanoscale, 3, 9, 89could be overcame by TD technique. 93, 2011
Corresponding Author: t.monti@univpm.it
[2] Hirose K., et al., Flash and smash: rapid freezing of muscle fibers activated by photolysis of caged ATP, Biophysical Journal, 65, 1, pp.397-408, 1993