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First Record of Renibacterium Salmoninarum in The Sea Lamprey
First Record of Renibacterium Salmoninarum in The Sea Lamprey
11/4/2013
Felicia Soth
11/4/2013
Results: R. salmoninarum was isolated from the collected sea lampreys extracted kidneys. It was not however found to be in the tested blood samples. Every isolate was tested to be gram-positive diplobacilli or coccobacilli. The colonies on the plates were white, shiny, smooth, had round colonies, and they were about 1mm in diameter. All retrieved isolates were confirmed with nPCR and Q-ELISA. With nPCR R. salmoninarum was detected in 66% of the kidneys of lampreys collected from Duffins creek and 5% from the Humber River. Discussion: This was the first report of the R. salmoninarum in the sea lamprey. Even though it was previously believed to be a salmonid-specific pathogen, it was found in sea lampreys from Lake Ontario for two consecutive years. While R. salmoninarum was found in the sea lampreys kidneys, it was not found to be in any other internal organ nor the blood. The isolates that had been found in the sea lampreys had the same attraction to kidneys as the R. salmoninarum found in salmonids. The size of the isolates found in the sea lampreys was the same size as what was published for R. salmoninarum. Tissues form the isolates retrieved was not consistent with the results. Conclusion: The reason for the tissues not being consistent may have been from bile salts that accumulated in the sea lampreys muscles and kidneys due to the fact that they have no gall bladder. Bile salts act like a detergent and may have been the reason for the inhibition on diagnostic assays. The once thought to be salmonine-specific R. salmoninarum is now beginning to jump species. In previous studies there had been no reports of the sea lamprey carrying R. salmoninarum. With this study there is proof that sea lampreys have some amounts of this disease. The sea lamprey can now be reported as a host of Renibacterium Salmoninarum. Limitations of this study: Due to the fact that the Sea Lamprey does not have a gall bladder makes it hard to perform diagnostic assays. This is because without a gall bladder bile salts build up within the muscles and kidneys which can cause an inhibition to nPCR and Q-ELISA. http://www.jwildlifedis.org/doi/pdf/10.7589/0090-3558-42.3.556
Felicia Soth
11/4/2013