Microbiology

Cat No. Unit of Sale

Gram Staining
Gram's Stain Set (500 mL) Contains 500 mL each of Crystal Violet, Gram's Iodine, Decolouriser and Safranin O As 621005, with dispenser spout Gram's Stain Set - Stabilised (500 mL) Contains 500 mL each of Crystal Violet, Stabilised Gram's Iodine, Decolouriser and Safranin O As 621015, with dispenser spout 621005 set

621006 621015

set set

621016

set

Carbol Fuchsin (Gram's) 0.05% Basic Fuchsin, 0.5% IMS, 0.225% Phenol

620135 620131 620130 620139 620155 620151 620150 620159 620505 620501 620500 620165 620160 620169 620855 620850 620615 620611 620610 620619 620535 620530 640525 640520 640529 620865 620860 620525 620520

500ml 1L 2.5L 5L 500ml 1L 2.5L 5L 500ml 1L 2.5L 500ml 1L 5L 500ml 1L 500ml 1L 2.5L 5L 500ml 1L 500ml 1L 5L 500ml 1L 500ml 1L

Crystal Violet (Gram's) 0.5% aqueous solution

Crystal Violet (Hucker-Conn) Crystal Violet 2% oxalated in 20% alcohol

Gram's Iodine Iodine 0.33%, Potassium Iodide 0.66%

Gram's Iodine (Stabilised) Iodine 0.33%, Potassium Iodide 0.66% + PVP Gram's Decolouriser (95% alcohol)

Jensen's Neutral Red Neutral Red 1% in 1% acetic acid Lugol's Iodine Iodine 1%, Potassium Iodide 2%

Lugol's Iodine (Stabilised) Iodine 1%, Potassium Iodide 2% + PVP Jensen's Methyl Violet Methyl Violet 6B 0.5% aqueous

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Microbiology
Cat No. Neutral Red (1% aqueous solution) 620225 620221 620220 620229 620245 620241 620240 620249 620515 620511 620510 Unit of Sale

500ml 1L 2.5L 5L 500ml 1L 2.5L 5L 500ml 1L 2.5L

Safranin O (1% aqueous solution)

Safranin O (Hucker-Conn) Safranin O 0.25% in 10% alcohol

Acid-fast staining
Kinyoun's "Cold" modified Ziehl-Neelsen Stain A widely used improvement on the classical techniques as it obviates the hazard of heating carbol fuchsin Kinyoun's Complete Set Contains 500 mL each of Kinyoun's Carbol Fuchsin and Methylene Blue with 2 x 500 mL acid alcohol Carbol Fuchsin (Kinyoun's) Basic Fuchsin 3%, Phenol 6.7% in 16% Alcohol 621045 kit

620605 620601 620600 620725 620721 620720 620585 620581 620580 620589 620595 620591 620590 620599

500ml 1L 2.5L 500ml 1L 2.5L 500ml 1L 2.5L 5L 500ml 1L 2.5L 5L

Lillie's Methylene Blue Methylene Blue 1%, Acetic Acid 1% in 19% alcohol

Acid Alcohol (3% HCl in IMS)

Acid-Acetone Alcohol HCl 5.7%, Acetone 3.5%, in 70% IDA-99 A stronger formulation for more rapid decolourising

Classical Ziehl-Neelen staining Ziehl-Neelsen Complete Set Contains 500 mL each of Z-N Carbol Fuchsin and Methylene Blue with 2 x 500 mL acid alcohol Carbol Fuchsin (Ziehl-Neelsen) Basic Fuchsin 1%, Phenol 4.5% in 10% Alcohol 621035 kit

620125 620121 620120 620985 620981 620980

500ml 1L 2.5L 500ml 1L 2.5L

Page 20

Gabett's Methylene Blue Methylene Blue 1%, Sulphuric Acid 20% in 30% alcohol

Microbiology
Cat No. Unit of Sale

Loeffler's Methylene Blue Methylene Blue 0.15% in 30% alcohol

620215 620211 620210 620205 620201 620200 620995 620991 620990 620195 620191 620190 620585 620581 620580 620589 620595 620591 620590 620599 620645 620641 620640

500ml 1L 2.5L 500ml 1L 2.5L 500ml 1L 2.5L 500ml 1L 2.5L 500ml 1L 2.5L 5L 500ml 1L 2.5L 5L 500ml 1L 2.5L

Methylene Blue (0.5% aqueous solution)

Sulphuric Methylene Blue Methylene Blue 0.5% in 20% Sulphuric Acid

Malachite Green (0.1% aqueous solution)

Acid Alcohol (3% HCl in IMS)

Acid-Acetone Alcohol HCl 5.7%, Acetone 3.5%, in 70% IDA-99 A stronger formulation for more rapid decolourising

Sulphuric Acid (20% aqueous solution)

Fluorescent stains for mycobacteria Auramine Staining Set Contains 500 mL each of Auramine O and Thiazine Red with 2 x 500 mL Decolouriser Auramine O (Lempert's) Auramine O 0.3% in 3% Phenol 621025 set

690135 690131 690130 690139 690375 690371 690370 690379 620555 620551 620550 620565 620561 620560

500ml 1L 2.5L 5L 500ml 1L 2.5L 5L 500ml 1L 2.5L 500ml 1L 2.5L

Auramine O Decolouriser Sodium Chloride 0.5%, Hydrochloric Acid 0.5% in 75% Alcohol

Auramine O & Rhodamine B Auramine O 0.12%, Rhodamine B 0.6% in 60% Glycerol / 8% Phenol Auramine O & Rhodamine B Decolouriser Hydrochloric Acid 0.5% in 70% Alcohol

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Microbiology
Cat No. Unit of Sale

Potassium Permanganate 0.5% aqueous solution

620575 620571 620570 620625 620621 620620 620629

500ml 1L 2.5L 500ml 1L 2.5L 5L

Thiazine Red Thiazine Red 0.05%, Magnesium Chloride 0.25%

Spore Stains
Carbol Fuchsin (Ziehl-Neelsen) Basic Fuchsin 1%, Phenol 4.5% in 10% Alcohol Ferric Chloride (30% aqueous solution) Malachite Green (5% aqueous solution) 620125 620120 622015 690515 690510 622035 622053 622055 620233 620235 622105 622095 500ml 2.5L 500ml 500ml 1L 500ml 30ml 500ml 30ml 500ml 500ml 500ml

Methylene Blue (1% aqueous solution) Nigrosine (1% aqueous in dropper bottle) Nigrosine (1% aqueous solution) Nigrosine (10% aqueous in dropper bottle) Nigrosine (10% aqueous solution) Safranin O (0.5% aqueous solution) Sodium Sulphite (5% aqueous solution)

Capsule Stains
Copper Sulphate (20% aqueous in dropper bottle) Copper Sulphate (20% aqueous solution) Crystal Violet (1% aqueous in dropper bottle) Crystal Violet (1% aqueous solution) Loeffler's Methylene Blue (in dropper bottle) Loeffler's Methylene Blue Methylene Blue 0.15% in 30% alcohol Safranin O (1% aqueous in dropper bottle) Safranin O (1% aqueous solution) 622073 622075 622063 622065 620213 620215 30ml 500ml 30ml 500ml 30ml 500ml

620243 620245

30ml 500ml

Other stains and reagents

Albert's Stain A Toludine Blue 0.15%, Malachite Green 0.2%, Acetic Acid 1% in 2% alcohol Albert's Stain B Iodine 0.33%, Potassium Iodide 0.66% 620105 620100 620115 620110 500ml 1L 500ml 1L
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Microbiology
Cat No. Unit of Sale

Crystal Violet (0.5% alcoholic solution)

692005 692000 620705

500ml 1L 500ml

Harris's Permanganate 1% aqueous solution Harris's Crystal Violet 2% aqueous solution Lactophenol 40% Glycerol, 20% Phenol, 20% Lactic Acid Lactophenol Blue (in dropper bottle) Lactophenol Blue Aniline Blue 0.075%, 40% Glycerol, 20% Phenol, 20% Lactic Acid Methylene Blue (Polychromed) Methylene Blue 1%, Potassium Carbonate 1%

620715

500ml

691795 691791 641633 641635 641630

500ml 1L 30ml 500ml 1L

641725 641721 641720 610301 610300

500ml 1L 2.5L 100ml 500ml

Neutral Mounting Medium (DPX) 10% Dibutylphthalate, 20% Styrene in Xylene NOTE: Contains phthalates Nigrosine (10% aqueous in dropper bottle) Nigrosine (10% aqueous solution)

620233 620235 620230 610993 610996 610991 610992 610990 691400 691405 622045 622040 620665

30ml 500ml 1L 30ml 60ml 100ml 250ml 500ml 30ml 500ml 500ml 1L 500ml

Clin-Tech 'High RI' Immersion Oil in dropper bottle Refractive Index = 1.513 - 1.518 Clin-Tech 'High RI' Immersion Oil Refractive Index = 1.513 - 1.518

Potassium Hydroxide (30% aqueous in dropper bottle) Potassium Hydroxide (30% aqueous solution) Pugh's Stain Toluidine Blue 0.2%, Acetic acid 5% in 2% alcohol Ruge's solution Formalin 2% in 1% acetic acid

Ruge's Mordant Tannic acid 5% aqueous Wayson's Stain Bipolar staining of Yersinia pestis

620675

500ml

620655 620650

500ml 1L

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Fertility Testing
Cat No. Unit of Sale

Sperm Vitality
Eosin Y (0.5% in 0.9% Saline, dropper bottle) Eosin Y (0.5% in 0.9% Saline) 625013 625015 625010 625023 625025 625020 625033 625035 625030 30ml 500ml 1L 30ml 500ml 1L 30ml 500ml 1L

Eosin Y (1% aqueous in dropper bottle) Eosin Y (1% aqueous solution)

Nigrosine (10% aqueous in dropper bottle) Nigrosine (10% aqueous solution)

Sperm Morphology
Bryan-Leishman method Bryan's Stain (Modified) 625045 625040 610225 610221 625055 625050 625061 625060 625071 625070 625080 500ml 1L 500ml 1L 500ml 1L 100ml 500ml 1L 2.5L pack

Leishman's Stain

Alpha-Naphthol Stock

Pyronin Y 0.1% in 96ml of 40% Alcohol + 4ml Aniline Sodium Citrate Buffer pH 7.5

Sorensen's Buffer pH 6.9 Supplied in concentrated liquid form. Dilute 5 mL to 1 litre - each pack contains 5 x 25 mL. Alcoholic Formalin

625095 625091 625090

500ml 1L 2.5L

Giemsa stain method Giemsa's Stain 0.6% Giemsa stain in 50% Methanol / 50% Glycerol 610255 610251 610250 625080 500ml 1L 2.5L pack

Sorensen's Buffer pH 6.9 Supplied in concentrated liquid form. Dilute 5 mL to 1 litre - each pack contains 5 x 25 mL.

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Fertility Testing
Cat No. Shorr's stain method Shorr's Stain Biebrich Scarlet 5%, Tungstophosphoric acid 5%, Molybdophosphoric acid 5%, Orange G 2.5%, Fast Harris's Haematoxylin (traditional, unacidified) Haematoxylin 0.45%, with Mercuric oxide, Potassium Aluminium Sulphate, in 4.5% alcohol Sorensen's Buffer pH 6.9 Supplied in concentrated liquid form. Dilute 5 mL to 1 litre - each pack contains 5 x 25 mL. Ammonium Alcohol 3% ammonia in 75% alcohol 641475 641471 641470 641625 641621 641620 625080 500ml 1L 2.5L 500ml 1L 2.5L pack Unit of Sale

625115 625111 625110

500ml 1L 2.5L

Papanicoloau's stain method Papanicolaou EA 50 A blend of Eosin Y, Light Green SF, Fast Green FCF, Bismark Brown, with PTA, in Acetic acid, 95% alcohol 640705 640701 640700 500ml 1L 2.5L

Papanicolaou Orange G Orange G 0.5%, Tungstophosphoric acid 0.015% in 95% alcohol Harris's acidified Haematoxylin (traditional) Haematoxylin 0.45%, with Mercuric oxide, Potassium Aluminium Sulphate, acetic acid in 4.5% alcohol Scott's Tapwater Substitute Magnesium Sulphate 2%, Sodium Hydrogen Carbonate 0.35% Acid Alcohol for semen examination 0.5% Hydrochloric acid in 75% alcohol

640725 640721 640720 640455 640451 640450 641281 641280 641289 625125 625121 625120

500ml 1L 2.5L 500ml 1L 2.5L 1L 2.5L 5L 500ml 1L 2.5L

Diluents for total spermatazoa counts Spermatozoa Diluent (colourless) Sodium Hydrogen Carbonate 5%, Formalin 2% aqueous solution Spermatozoa Diluent (Trypan Blue) Sodium Hydrogen Carbonate 5%, Formalin 2%, Trypan Blue 0.025% aqueous solution 625105 625101 625100 625135 625131 625130 500ml 1L 2.5L 500ml 1L 2.5L

Page 25

Parasitology
Cat No. Unit of Sale

Stains recommended by NEQAS for faecal parasites

Romanowsky stains Field's Stain Slide Kit Giemsa's Stain Modified Ziehl-Neelsen stains Carbol Fuchsin (Kinyoun's) Malachite Green (Counterstain) 0.5% aqueous solution Malachite Green (5% aqueous solution) Methylene Blue (0.5% aqueous solution) Acid Alcohol (3% HCl in IMS) Modified Trichrome Stains Ryan-Blue modified Trichrome Chromotrope 2R 6%, Aniline Blue 0.5%, Phosphotungstic acid 0.25% in 3% Acetic acid Weber's modified Trichrome Chromotrope 2R 6%, Phosphotungstic acid 0.7%, Fast Green 0.3% in 3% Acetic acid Wheatley's modified Gomori's Trichrome Chromotrope 2R 0.6%, Phosphotungstic acid 0.7%, Light Green 0.3% in 1% Acetic acid Acetic acid Alcohol 0.5% 0.5% Acetic acid in 90% Alcohol Acetic acid Alcohol 4.5% 4.5% Acetic acid in 90% Alcohol Phenol Auramine stain Auramine O (Lempert's) Auramine O Decolouriser Thiazine Red Potassium Permanganate 0.1% aqueous solution 690135 690375 620625 621055 500ml 500ml 500ml 500ml 621545 500ml 620605 629015 500ml 500ml 611000 610255 kit 500ml

690515 620205 620585

500ml 500ml 500ml

621535

500ml

621525

500ml

621095

500ml

621105

500ml

Page 26

Parasitology
Cat No. Unit of Sale

Stains recommended by CDC for faecal parasites

Chromotrope staining procedure This technique uses a higher concentration of chromotrope 2R than most other methods Chromotrope Stain Chromotrope 2R 0.6%, Phosphotungstic acid 0.7%, Fast Green 0.15% in 3% Acetic acid Acetic acid Alcohol 0.45% Quick-Hot Gram - Chromotrope staining procedure Chromotrope Stain (Hot Gram) Chromotrope 2R 1%, Phosphotungstic acid 0.25%, Fast Green 0.15% in 1% Acetic acid Crystal Violet (Gram's) Gram's Iodine Gram's Decolouriser (95% alcohol) Acetic acid Alcohol 0.45% 0.45% acetic acid in 90% alcohol Modified Safranin Technique (hot method) Safranin O (1% aqueous solution) Malachite Green (3% aqueous solution) Acid Methanol 3% 3% Hydrochloric acid in Methanol 620245 621135 621115 500ml 500ml 500ml 621085 500ml 621065 500ml

621075

500ml

620155 620165 620615 621075

500ml 500ml 500ml 500ml

Stool Fixative, Preservative and Concentration Fluids

Formalin PBS 1.5% formalin in Phosphate Buffered Saline 621245 621241 621240 621225 621221 621220 621235 621231 621230 500ml 1L 2.5L 500ml 1L 2.5L 500ml 1L 2.5L

PVA fixative (zinc formulation) Mercury free

SAF (Sodium Acetate Formalin) Formaldehyde 4%, Triton X-100 0.1% in pH 4.2 Sodium acetate / acetic acid buffer

Page 27

Parasitology
Cat No. Unit of Sale

Fluorescent stains for Trichomonas vaginalis

Acridine Orange is recommended as a rapid method for screening routine material from the female genital tract. Acridine Orange 0.01% pH 3.5-4.0 working solution Can be used as a rapid method for screening for Trichomonas vaginalis Acridine Differentiating Fluid Sodium Chloride 0.8% in 2% alcohol Acridine phosphate buffered saline pH 7.2 Holding solution 620542 620540 250ml 1L

620745 620740 620755 620750

500ml 1L 500ml 1L

Page 28