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Penicillium Chrysogenum
Penicillium Chrysogenum
Penicillium Chrysogenum
Penicillium chrysogenum is a fungus, common in temperate and subtropical regions and can be found on salted food products,[1] but it is mostly found in indoor environments, especially in damp or waterdamaged buildings.[2] It was previously known as Penicillium notatum.[3] It has rarely been reported as a cause of human disease. It is the source of several -lactam antibiotics, most significantly penicillin. Other secondary metabolites of P. chrysogenum include various penicillins, roquefortine C, meleagrin, chrysogine, xanthocillins, secalonic acids, sorrentanone, sorbicillin, and PR-toxin.[4] Like the many other species of the genus Penicillium , P. chrysogenum usually reproduces by forming dry chains of spores (or conidia) from brush-shaped conidiophores. The conidia are typically carried by air currents to new colonisation sites. In P. chrysogenum the conidia are blue to blue-green, and the mold sometimes exudes a yellow pigment. However, P. chrysogenum cannot be identified based on colour alone. Observations of morphology and microscopic features are needed to confirm its identity and DNA sequencing is essential to distinguish it from closely related species such as Penicillium rubens. The sexual stage of P. chrysogenum was discovered in 2013 by mating cultures in the dark on oatmeal agar supplemented with biotin, after the mating types (MAT1-1 or MAT1-2) of the strains had been determined using PCR amplification.[5] The airborne asexual spores of P. chrysogenum are important human allergens. Vacuolar and alkaline serine proteases have been implicated as the major allergenic proteins.[6] P. chrysogenum has been used industrially to produce penicillin and xanthocillin X, to treat pulp mill waste, and to produce the enzymes polyamine oxidase, phospho-gluconate dehydrogenase, and glucose oxidase.[4][7]
At this point, though the drug had shown success in treating numerous bacterial diseases, it was still so difficult to produce and so dilute that it was not feasible to produce quantities large enough for mass production, and so an effort was begun to find a strain of Penicillium with a higher rate of production of penicillin. Army pilots sent back soil from around the world to be tested for the right kind of mold.[citation needed] Even the people of Peoria, Illinois were told to bring in any molds that they found around their homes. [citation needed ] It has also been said[where?] that the scientists working on this project kept an eye out for similar- looking molds while grocery shopping or when they were cleaning around the kitchen especially their refrigerators.[citation needed ] It was by these means that Penicillium chrysogenum was discovered, on a cantaloupe from a grocery store in Peoria, Illinois.[11] The fungus isolated from this cantaloupe produced several hundred times as much penicillin as Fleming's original cultures of Penicillium notatum . Subcultures of this fungus were then irradiated with X-rays and UV rays in an attempt to cause a mutation in the fungus that would lead to an increase in penicillin yield. The effort was successful, and a mutant strain that yielded more than a thousand times the penicillin of Fleming's original culture was produced and cultured. [citation needed] This discovery, in combination with vastly improved methods of culturing the fungus based on the principle of aerating the culture medium, resulted in the ability to mass-produce penicillin in quantities great enough for distribution and mass use in the United States Army, and later within the British armed service and hospitals, in WWII. The discovery of penicillin ushered in a new age of antibiotics derived from microorganisms. Penicillin is an antibiotic made by growing the mold Penicillium chrysogenum in a fermenter. The mold is grown in a liquid culture containing sugar and other nutrients including a source of nitrogen. As the mold grows, the sugar is used up and starts to make penicillin only after using up most of the nutrients for growth.