Results and Calculations:

Figure 1: Absorbance vs Time(min) for no inhibitor data sets:

Table 1: Equations of lines of best fit:
Data Set: Equation of line of best fit
1 Set 1: y = 0.0489x + 0.2038 R = 0.9852
2 Set 2: y = 0.0698x + 0.2496 R = 0.998
3 Set 3: y = 0.1062x + 0.291 R = 0.9961
4 Set 4: y = 0.1548x + 0.3903 R = 0.9874
5 Set 5: y = 0.1509x + 0.391 R = 0.9408

Table 2: Rates of Reactions:






0
0.1
0.2
0.3
0.4
0.5
0.6
0 0.2 0.4 0.6 0.8 1 1.2
A
b
s
o
r
b
a
n
c
e

Time (min)
Absorbance vs Time (No Inhibitor)
Set 1
Set 2
Set 3
Set 4
Set 5
Linear (Set 1)
Linear (Set 2)
Linear (Set 3)
Linear (Set 4)
Linear (Set 5)
Data Set Rate of Reaction
(A/min)
1 0.0489
2 0.0698
3 0.1062
4 0.1548
5 0.1509
Figure 2: Plot of Absorbance vs Time(min) for data sets with inhibitor:

Table 3: Equations of lines of best fit:
Data sets: Equations of lines:
1 y = 0.057x + 0.3207 R = 0.9882
2 y = 0.0657x + 0.2338 R = 0.9975
3 y = 0.1128x + 0.3559 R = 0.9971
4 y = 0.0943x + 0.3247 R = 0.9839
5 y = 0.1374x + 0.5274 R = 0.998

Table 4: Rates of Reactions:





0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 0.2 0.4 0.6 0.8 1 1.2
A
b
s
o
r
b
a
n
c
e

Time (min)
Absorbance vs Time (with inhibitor)
Set 1
Set 2
Set 3
Set 4
Set 5
Linear (Set 1)
Linear (Set 2 )
Linear (Set 3)
Linear (Set 4)
Linear (Set 5)
Data Sets Rate of Reaction
(A/min)
1 0.057
2 0.0657
3 0.1128
4 0.0943
5 0.1374
Table 5: Velocities of the data sets and the reciprocal velocities:
Data Sets: V
o
(mM/min) 1/V
o
(min/mM)
No
inhibitor
1 0.01358 73.64
2 0.01940 51.55
3 0.0295 33.90
4 0.043 23.26
5 0.0419 23.87
With
Inhibitor
1 0.0158 63.29
2 0.0183 54.64
3 0.031 32.26
4 0.0262 38.17
5 0.0382 26.18

Sample calculation: Converting A/min to V
o
:
Using Beer's law: A =cl where A = A/min, =3600 L/mol cm, l = 1cm, x 1000mM/1M to convert units
Using Set 1 of no inhibitor:
V
o
= c = A/l = [(0.0489 min
-1
)/(3600 L/mol cm)(1 cm)] x (1000mM/1M)
V
o
= 0.01358 mM/min
Table 6: Substrate Calculations and the reciprocal Concentrations:
Data Sets: Substrate Concentration [S] (mM) Reciprocal Substrate Concentration 1/[S]
(mM
-1
)
No
inhibitor
1 0.8 1.25
2 1.6 0.625
3 2.4 0.417
4 3.2 0.3125
5 4.0 0.25
With
Inhibitor
1 0.8 1.25
2 1.6 0.625
3 2.4 0.417
4 3.2 0.3125
5 4.0 0.25

Sample Calculations: Calculating Substrate Concentrations [S]:
Using C
1
V
1
=C
2
V
2
where C
1
= 8 mM, V
1
= 100L, V
2
= 1000L
Solving for C
2
: C
2
= C
1
V
1
/V
2
= (8)(100)/1000 = 0.8 mM

Figure 3: Michaelis-Menten Plot of Tyrosinase-Catalyzed Reactions:

Figure 4: Lineweaver-Burk plot of Tyrinosinase-Catalyzed Reactions:

Without Inhibitor:
y = 0.01x + 0.0054
R = 0.9311
With Inhibitor:
y = 0.0066x + 0.0101
R = 0.8237
0
0.01
0.02
0.03
0.04
0.05
0 1 2 3 4 5
V
o

(
m
M
/
m
i
n
)

Substrate Concentration [S] (mM)
Michaelis-Menten Plot of
Tyrosinase-Catalyzed Reactions
No Inhibitor
with Inhibitor
Without Inhibitor:
y = 51.572x + 11.802
R = 0.9531
With Inhibitor:
y = 34.534x + 23.193
R = 0.8095
0
10
20
30
40
50
60
70
80
90
0 0.5 1 1.5
1
/
V
o

(
m
i
n
/
m
M
)

Recipricol of Substrate Concentration 1/[S] (mM
-1
)
Lineweaver-Burk Plot of
Tyrosinase-Catalyzed Reactions
No Inhibitor
with Inhibitor