Anemia is associated with an increased risk of mortality in both systolic and diastolic CHF. Of the 153,180 CHF patients, 37.2% were anemic. Lower baseline hemoglobin values were associated with increased crude mortality rates.
Anemia is associated with an increased risk of mortality in both systolic and diastolic CHF. Of the 153,180 CHF patients, 37.2% were anemic. Lower baseline hemoglobin values were associated with increased crude mortality rates.
Anemia is associated with an increased risk of mortality in both systolic and diastolic CHF. Of the 153,180 CHF patients, 37.2% were anemic. Lower baseline hemoglobin values were associated with increased crude mortality rates.
A Systematic Review and Meta-Analysis Hessel F. Groenveld, MD,* James L. Januzzi, MD, FACC, Kevin Damman, MD,* Jan van Wijngaarden, MD, PHD, Hans L. Hillege, MD, PHD,* Dirk J. van Veldhuisen, MD, PHD, FACC,* Peter van der Meer, MD, PHD Groningen and Deventer, the Netherlands; and Boston, Massachusetts Objectives The aim of this study was to assess the effect of anemia on mortality in chronic heart failure (CHF). Background Anemia is frequently observed in patients with CHF, and evidence suggests that anemia might be associated with an increased mortality. Methods A systematic literature search in MEDLINE (through November 2007) for English language articles was per- formed. In addition, a manual search was performed. We included cohort studies and retrospective secondary analyses of randomized controlled trials whose primary objective was to analyze the association between ane- mia and mortality in CHF. Of a total of 1,327 initial studies, we included 34 studies, comprising 153,180 pa- tients. Information on study design, patient characteristics, outcome, and potential confounders were extracted. Results Anemia was dened by criteria used in the original articles. Of the 153,180 CHF patients, 37.2% were anemic. After a minimal follow-up of 6 months, 46.8% of anemic patients died compared with 29.5% of nonanemic pa- tients. Crude mortality risk of anemia was odds ratio 1.96 (95% condence interval: 1.74 to 2.21, p 0.001). Lower baseline hemoglobin values were associated with increased crude mortality rates (r 0.396, p 0.025). Adjusted hazard ratios showed an increased adjusted risk for anemia (hazard ratio 1.46 [95% con- dence interval: 1.26 to 1.69, p 0.001]). Subgroup analysis showed no signicant difference between mortality risk of anemia in diastolic or systolic CHF. Conclusions Anemia is associated with an increased risk of mortality in both systolic and diastolic CHF. Anemia should, therefore, be considered as a useful prognosticator, and therapeutic strategies aimed to increase hemoglobin levels in CHF should be investigated. (J Am Coll Cardiol 2008;52:81827) 2008 by the American College of Cardiology Foundation Anemia is frequently observed in patients with chronic heart failure (CHF) (15). Prevalence of anemia depends both on the severity of CHF and diagnostic criteria used to dene it, but may be as high as 50% in selected patient cohorts (3,6). Of note, anemia is not only prevalent in the CHF population, but several studies in different patient populations found an asso- ciation with anemia, impaired cardiac function, more health care utilization, and morbidity (1,711). In addition, numerous studies have assessed associations between anemia and mortal- ity in CHF. Although most studies have documented higher mortality rates in anemic CHF patients (1,3,1217), some studies report the absence of an adverse effect of anemia on mortality in CHF (4,1820). Therefore, better understanding of the risk associated with the presence of anemia is necessary. Elucidating the contribution of anemia to mortality may lead to a more accurate risk proling in CHF patients, especially since several treatment options for anemia exist including erythropoietin (EPO) and iron therapy (21,22). Ultimately, this may lead to more effective therapeutic strategies from a risk-benet perspective in heart failure patients. We, therefore, performed a rigorous systematic review and meta-analysis of published literature to more compre- hensively assess the effect of anemia on mortality in CHF. In addition, we explored whether subgroups of anemic patients were at particularly high mortality risk. Methods Search strategy. We adhered to the MOOSE (Meta- analysis Of Observational Studies in Epidemiology) study guidelines as previously published (23). To identify all relevant From the *Department of Cardiology, University Medical Center Groningen, Groningen, the Netherlands; Division of Cardiology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts; and the Department of Cardiology, Deventer Hospital, Deventer, the Netherlands. Dr. Damman is supported by the Netherlands Heart Foundation (grant 2006B157). Dr. van Veldhuisen is a Clinical Established Investigator of the Netherlands Heart Foundation (D97-017). Dr. van der Meer is supported by the Dutch Scientic Organization (Rubicon grant: 825-07-011). Manuscript received February 19, 2008; revised manuscript received April 23, 2008, accepted April 28, 2008. Journal of the American College of Cardiology Vol. 52, No. 10, 2008 2008 by the American College of Cardiology Foundation ISSN 0735-1097/08/$34.00 Published by Elsevier Inc. doi:10.1016/j.jacc.2008.04.061 Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 studies, we performed a literature search in MEDLINE accessed by PubMed (1966 throughNovember 2007). Two of the authors (H.G. and P.v.d.M.) developed a search strategy. To identify heart failure patients and anemia, we used both the Medical Subject Heading term (MeSH) and text word search (Table 1). In addition, we identied potentially relevant studies using a manual search of references lists from all eligible studies and review articles. We consulted experts in the eld and searched the Institute for Scientic Informa- tion Web of Sciences for publications that cited key publications. Study selection. We included cohort studies and retro- spective secondary analyses of randomized controlled trials whose primary objective was to analyze the association between anemia and mortality in CHF. Titles and abstracts of all articles were evaluated and rejected on initial screen if they: 1) included subjects other than CHF patients; 2) had no evaluation of hemoglobin (Hb) or hematocrit (Ht) levels; 3) did not include all-cause mortality as outcome; 4) were published only in abstract form; 5) included patients 18 years old; 6) had follow-up of 6 months; or 7) were published in another language than English. After obtaining full reports of candidate studies, the same reviewers independently assessed eligibility. Differences in data between the 2 reviewers were solved by rereviewing corresponding articles, and the nal set was agreed on by consensus. For studies in which not all data were published, or data was insufcient, authors were addressed and asked for additional information (3,4,12,19,2426). Quality assessment and data abstraction. Each study was evaluated on quality according to the guidelines provided by the United States Preventive Task Force (27) and published recommendations (28). The following characteristics were assessed: 1) duration of follow-up 12 months; 2) reporting loss of follow-up; 3) adjustment of possible confounders in multivariate analysis; 4) denition of anemia; 5) full speci- cation of outcome; 6) study sample representative for mentioned population; 7) full specication of clinical and demographic variables; 8) explanation of sample selection; 9) temporality (Hb measured at baseline, not at time of outcomes assessment); and 10) clear inclusion and exclusion criteria. Studies were graded as poor quality if they met 5 criterion, fair if they met 5 to 7 criteria, and good if they met 8 criteria. Statistical analysis. Random effects meta-analysis was con- ducted to estimate the magni- tude of risk associated with ane- mia and all-cause mortality, as measured by crude mortality risks and unadjusted odds ratios (ORs). Where available, adjusted hazard ratios (HRs) were ex- tracted from Cox regression analysis. Adjusted risk estimates included those components as published in nal multivariate models for each study including confounding sociodemo- graphic and clinical covariates. We performed tests of heterogeneity between studies using a standard chi-square test and I 2 statistic. When heterogeneity was found to be signicant, pooled estimates based on random effects mod- els were reported. To examine sources of heterogeneity, we conducted meta-regression analysis. Bias in published stud- ies was assessed using a funnel plot of study results against study precision. We tested symmetry of the funnel plot by the Eggers test. Subgroup analysis was pre-dened for systolic versus diastolic CHF. Statistical signicance was set at p 0.05, and all statistical analyses were performed using Stata 9.0 (Stata Corp., College Station, Texas). Results Description of included studies. The electronic search retrieved 1,327 eligible studies. Nonelectrical search identi- ed 4 additional studies. On initial screening, 1,142 were rejected, based on title. Of the 185 screened abstracts, 56 studies were retrieved for detailed evaluation. Eventually 34 studies were included in this meta-analysis (Fig. 1). Sec- ondary analysis of the Val-HeFT (Valsartan Heart Failure Trial) study was described in 2 separate manuscripts: Anand et al. (29) and Maggioni et al. (5). Crude mortality data were not published by Anand et al. (29), and for this reason this study was excluded and the data of Maggioni et al. (5) was used. In addition, 1 article described the effect of anemia from 2 different studies (5). Both studies in this published report were analyzed separately for the meta- analysis. The 34 included studies described a total of 153,180 patients (1,320,2426,3041). Characteristics of the included studies are listed in Table 2, characteristics of study populations are listed in Online Tables 1 and 2. Ten different denitions of anemia were used in the included studies. The World Health Organization (WHO) deni- tion (Hb 13.0 g/dl for males and Hb 12.0 g/dl for females) was used in the majority of the studies. Table 3 displays the different denitions of anemia in the included studies. Anemia, as dened in the original articles, was Terms Used in the Search Query Table 1 Terms Used in the Search Query Text words Congestive heart failure, CHF, anemia, anaemia, hemoglobin, haemoglobin, Hb, Hgb, hematocrit, haematocrit, Ht, Hct MeSH terms Heart failure, congestive; anemia; hemoglobin; hematocrit Title and abstract search Hemoglobins Limits English language CHF congestive heart failure; MeSH Medical Subject Heading. Abbreviations and Acronyms CHF chronic heart failure CI condence interval EPO erythropoietin ESP erythropoiesis- stimulating protein Hb hemoglobin HR hazard ratio Ht hematocrit OR odds ratio WHO World Health Organization 819 JACC Vol. 52, No. 10, 2008 Groenveld et al. September 2, 2008:81827 Anemia and Mortality in Heart Failure Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 present in 37.2% of the CHF patients. Mean Hb from the studies ranged from 12.2 to 14.0 g/dl. Mean Ht values ranged from 36.6% to 42.7%. Meta-analysis ndings. Among the 34 studies, mean follow-up ranged from a minimum of 6 months to a maximum of 5 years. Crude mortality data were available in 33 studies. In one study, only the adjusted HR was available, whereas the unpublished crude mortality data were lost after hurricane Katrina (25). Analysis of the crude mortality revealed that 26,687 (46.8%) anemic patients died com- pared with 28,274 (29.5%) nonanemic patients. This trans- lated into an unadjusted mortality risk of OR 1.96 (95% condence interval [CI]: 1.74 to 2.21, p 0.001) in anemic CHF patients compared with that in nonanemic CHF patients (Fig. 2). When the 2 largest studies (Go et al. [3] and Kosiborod et al. [4]) were excluded, the mortality risk of anemia was identical (OR: 1.95, 95% CI: 1.78 to 2.14, p 0.001). There was no evidence for publication bias. The funnel plot did not show asymmetry (Fig. 3), which was conrmed by the Eggers test (p 0.93). We found a signicant heterogeneity between included studies (I 2
92.4%, p 0.001). Meta-regression analysis was performed
using sociodemographic variables, medical history, drug use, and laboratory data as parameters. In this sensitivity analy- sis, excluding studies not adhering to the WHO denition of anemia, no heterogeneity was observed (I 2 36.5%, p NS), yet the mortality risk remained (OR: 2.22, 95% CI: 2.04 to 2.42, p 0.001). In addition, we found an inverse interstudy relationship between serum creatinine levels and the effect of anemia on mortality. The effect of anemia on mortality declined with higher serum creatinine levels. When assessing the gradual effect of baseline Hb values on mortality, we found a linear trend between baseline Hb measurements and mortality rates. Lower baseline Hb values were associated with increased annual mortality rates (r 0.396, p 0.025) (Fig. 4). Confouding factors. In 127,437 patients (83.1% of the total included patients), reported mortality risks were ad- justed for several sociodemographic and clinical covariates. Potential confounders that were adjusted for in the original reports are shown in Table 4. In all but one study, age and renal function were considered as potential confounders (this study included 178 patients) (33). When mortality risk, estimated by adjusted HR, was combined, anemia remained an independent predictor of mortality HR 1.46 (95% CI: 1.26 to 1.69, p 0.001). Subgroup analysis. In the subgroup analysis, we assessed the difference between mortality risk of anemia in patients with systolic or diastolic CHF. Table 5 displays the deni- tions of systolic and diastolic CHF used in the original articles. Studies or substudies in patients with only diastolic CHF included 20,924 patients of which 5,957 (28.5%) patients died. In studies or substudies including only pa- tients with systolic CHF, 40,025 patients were included, of which 12,423 (31.0%) died. The mortality risk associated with the presence of anemia was not signicantly different between patients with systolic CHF (OR: 1.96, 95% CI: 1.70 to 2.25, p 0.001) and diastolic CHF (OR: 2.09, 95% CI: 1.53 to 2.86, p 0.001). Discussion This is the rst meta-analysis to address the relationship between anemia and mortality in patients with CHF. In our analysis, which examined more than 150,000 subjects, anemia was frequently observed, found in over one-third of CHF patients. Presence of anemia in CHF patients is associated with an increased mortality risk in patients with systolic as well as diastolic heart failure. The adverse effect we found is substantial and signicant. When assessing the mortality risk by using multivariate analyses, anemia re- mains an independent risk factor for mortality in CHF patients. Prevalence. In prior studies, it is observed that CHF is often accompanied by anemia. A wide range of anemia prevalence in CHF has been reported, ranging from 7% to Figure 1 Flow Chart for Study Selection CHF chronic heart failure; Hb hemoglobin. 820 Groenveld et al. JACC Vol. 52, No. 10, 2008 Anemia and Mortality in Heart Failure September 2, 2008:81827 Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 Study Characteristics Table 2 Study Characteristics Author (Ref. #), Year Follow-Up n Patients End Points Major Exclusion Criteria Study Design Study Al-Ahmad et al. (12), 2001 33.4 months 6,360 CHF, LVEF 35%, age 21 to 81 yrs Mortality Recent MI, unstable angina, sCR 2.5 mg/dl Secondary analysis of clinical trial (SOLVD) Good Horwich et al. (9), 2002 5 yrs 1,061 Referred for heart transplantation evaluation Mortality LVEF 40%, NYHA class III, no initial Hb, dry weight, or adequate follow-up Retrospective, observational, single-center cohort study Good McClellan et al. (38), 2002 2 yrs 633 Discharge diagnosis of HF (ICD-9-CM) Mortality Dialysis, death during index hospitalization, missing records Retrospective, observational, multicenter cohort study Good Tanner et al. (32), 2002 6 months 193 Outpatient HF clinic at university hospital Mortality, heart transplantation Secondary anemia, hemodialysis, coronary intervention last 3 months Retrospective, observational, single-center cohort study Good Kalra et al. (26), 2003 3 yrs 531 HF Mortality Prospective observational, single-center cohort study Fair Kosiborod et al. (39), 2003 1 yr 2,281 Principal discharge diagnosis of HF (ICD-9-CM), age 65 yrs Mortality, hospitalization Severe AoS or MiS, HF due to acute illness, missing Htc values, in-hospital mortality Retrospective, observational, multicenter chart review Good Mozaffarian et al. (37), 2003 15 months 1,130 LVEF 30% and NYHA class IIIB or IV HF symptoms Mortality MI; unstable angina; stroke or revascularization; severe pulmonary, renal, or hepatic disease, sCR 3.0 mg/dl Secondary analysis of multicenter clinical trial (PRAISE) Good Kerzner et al. (40), 2003 25 months 367 Primary discharge diagnosis of HF Mortality Retrospective, observational, single-center chart review Fair Szachniewicz et al. (33), 2003 529 days 176 Admitted for HF Mortality Anemia due to known secondary cause Prospective, observational, single-center, cohort study Good Sharma et al. (34), 2004 551 days 3,044 NYHA II to IV, LVEF 40%, age 60 yrs Mortality Severe valvular disease, recent PTCA, CABG, MI, or unstable angina, sCR 220 mol/l Secondary analysis of multicenter clinical trial (ELITE II) Good Anand et al. (1), 2004 12.7 months 912 NYHA II to IV, LVEF 30%, age 18 to 55 yrs Mortality, hospitalization Surgically correctable causes of HF, other serious illness, recent or planned revascularization Secondary analysis of clinical trial (RENAISSANCE) Good van der Meer et al. (30), 2004 1,100 days 74 Mild-to-severe CHF Mortality MI, CVA, severe renal failure, valvular disease, isolated systolic dysfunction Retrospective, observational, single-center cohort study Good Ezekowitz et al. (24), 2005 962 days 791 Referred for assessment and management of CHF Mortality Missing Hb values Prospective observational, single-center cohort study Good Maggioni et al. (5), Val-HeFT 2005 23 months 5,010 Symptomatic HF (NYHA II), clinically stable Mortality, time to death, rst morbid event Rapidly deteriorating HF, acute MI, CAD likely to require intervention, unstable angina, recent CVA, sCR 2.5 mg/dl Secondary analysis of a randomized clinical trial (Val-HeFT) Good Maggioni et al. (5), IN-CHF 2005 1 yr 2,411 Diagnosis of HF according to European Society of Cardiology Mortality Retrospective, observational, multicenter cohort study Good Gardner et al. (19), 2005 554 days 182 Advance HF, referred for cardiac Tx assessment Mortality Age 16 yrs, pregnancy, known malignancy Prospective, observational, single-center cohort study Fair Continued on next page 821 JACC Vol. 52, No. 10, 2008 Groenveld et al. September 2, 2008:81827 Anemia and Mortality in Heart Failure Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 Continued Table 2 Continued Author (Ref. #), Year Follow-Up n Patients End Points Major Exclusion Criteria Study Design Study Rosolova et al. (35), 2005 4 yrs 508 Suspected HF Mortality Retrospective, observational, multicenter cohort study Fair Grigorian et al. (14), 2006 1.5 yrs 205 Admitted to tertiary center for CHF Mortality LVEF 50% Retrospective, observational, Single-center cohort study Good Kosiborod et al. (4), 2005 1 yr 50,405 Discharge diagnosis of HF Mortality, hospitalization for HF Patients without documented HF on admission, age 65 yrs, hemodialysis, severe Aos, MiS, Retrospective, observational, multicenter cohort study Good Ralli et al. (16), 2005 1 yr 264 Referred to a university hospital for HF management or heart Tx Mortality ACS or myocarditis, LVEF 40% Retrospective, observational, single-center cohort study Good OMeara et al. (36), 2006 37.7 months 2,653 Symptomatic (NYHA II to IV) HF, for at least 4 weeks, age 18 yrs Mortality sCR 265 mol/l, symptomatic HT, MI, CVA Secondary analysis of clinical trial (CHARM) Good Felker et al. (13), 2006 3.6 yrs 4,951 Symptomatic HF (NYHA II) patients undergoing cardiac catheterization Mortality Primary valvular heart disease or congenital heart disease Retrospective, observational, single-center cohort study Fair Terrovitis et al. (31), 2006 2 yrs 160 Symptomatic HF (NYHA II to IV), LVEF 35% Mortality Recent MI or CABG, unstable angina, anticipated cardiac surgery, sCR 3 mg/dl Secondary analysis of multicenter, prospective clinical trial Good Hebert et al. (25), 2006 436 days 410 LVEF 40%, enrolled in chronic HF disease managemant program Mortality Missing baseline values of Hb, sCR Retrospective, observational, single-center cohort study Fair Formiga et al. (18), 2006 1 yr 103 Admitted for symptomatic, previously unknown HF Mortality, hospitalization for HF Chronic advanced disease, COPD, advanced renal failure hemodialysis, in-hospital mortality Prospective, observational, single-center cohort study Good Newton and Squire (15), 2006 1,257 days 528 Discharge diagnosis of HF, without previous hospitalization for HF Mortality Insufcient evidence of new diagnosis of CHF Retrospective, observational, single-center chart review Good Komajda et al. (10), 2006 58 months 2,996 NYHA class II to IV and LVEF 35% Mortality, hospitalization Recent change of treatment, requirement of IV medication Secondary analysis of clinical trial (COMET) Good Go et al. (3), 2006 2 yrs 59,772 Diagnosed with CHF Mortality, hospitalization Retrospective observational, longitudinal cohort study Good Berry et al. (7), 2006 814 days 519 Diagnosed with CHF or a treatment for CHF Mortality, hospitalization, HF hospitalization Primary or secondary diagnosis of acute MI Retrospective, observational, single-center chart review Fair de Silva et al. (42), 2006 531 days 955 LVEF 45%, CHF enrolled in a community-based program Mortality Pregnancy, dialysis, disorders resulting in anemia Prospective, observational, single-center cohort study Fair Maraldi et al. (11), 2006 12 months 567 Diagnosed with HF, age 65 yrs Mortality, physical disability Physical disability at hospital discharge, dementia, or severe cognitive impairment Secondary analysis of longitudinal observational study Fair Elabbassi et al. (8), 2006 1.3 yrs 437 Systolic or diastolic CHF Mortality, hospitalization, ED visit No complete follow-up Prospective observational, single-center cohort study Good Continued on next page 822 Groenveld et al. JACC Vol. 52, No. 10, 2008 Anemia and Mortality in Heart Failure September 2, 2008:81827 Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 over 50% (14,10,17,32,35). This range can be attributed to multiple factors, including the use of multiple denitions of anemia. The WHO criteria was used in the majority of studies. In addition, administrational codes or arbitrary boundary values to dene anemia were also used. Studies dening anemia with administrative codes (such as Inter- national Classication of Diseases, 9th Revision, codes) have clear limitations. The use of this coding system solely relies on physicians to consider anemia as a possible diagnosis leading to an underestimation of the preva- lence. Therefore, we excluded in this meta-analysis stud- ies without measurements of actual Hb or Ht values. This resulted in the exclusion of only one study (2). Results of the current meta-analysis clearly indicate the need for standardized denitions of anemia in CHF patients. The denition of anemia appeared to be a major source of heterogeneity. However, despite different denitions of anemia in the present meta-analysis, the association with mortality was robust, using different analysis strategies. Pathophysiology. It is tempting to speculate about the mechanisms behind the increased mortality risk observed in anemic CHF patients. Anemia may lead to an increased work load, resulting from an increased heart rate and stroke volume (32,42,43). In response to the increased workload, the heart undergoes remodeling, marked by left ventricu- lar hypertrophy and dilation. This eventually may lead to CHF with an increased mortality risk. Also, renal failure, as a common comorbidity in CHF, might be implicated in the pathophysiology. CHF and renal failure are 2 entities that are often seen together with prevalences ranging from 20% to 40% (2,38,4446). With meta-regression analysis, we found an inverse interstudy relationship between serum creatinine levels and the effect of anemia on mortality. However, this result does not suggest that the mortality risk declines with higher creatinine levels. It merely illustrates a statistical decreasing effect of anemia on mortality with increasing creatinine levels; the decline of the effect of anemia on mortality can be the result of an increased Denitions of Anemia Used in the Original Articles Table 3 Denitions of Anemia Used in the Original Articles Denition of Anemia Author (Ref. #) WHO Elabbassi et al. (8), Maraldi et al. (11), De Silva et al. (42), Go et al. (3), Komajda et al. (10), Formiga et al. (18), Hebert et al. (25), OMeara et al. (36), Felker et al. (13), Ralli et al. (16), Grigorian Shamagian et al. (14), Rosolova et al. (35), Gardner et al. (19), Ezekowitz et al. (24), van der Meer et al. (30), Kerzner et al. (40), Mozaffarian et al. (37), Schou et al. (17) Hb 11.5 g/dl Kalra et al. (26) Hb 12.0 g/dl Varadarajan et al. (6), Anand et al. (1), Szachniewicz et al. (33), Tanner et al. (32) Hb 12.3 g/dl Horwich et al. (9) Hb 13.0 g/dl Sharma et al. (34) Hb 11.0 g/dl in women Maggioni et al. (Val-HeFT) (5), Maggioni et al. (IN-CHF) (5) and Hb 12.0 g/dl in men Hb 11.5 g/dl in women Berry et al. (7), Newton and Squire (15) and 13.0 g/dl in men Ht 35% Al-Ahmad et al. (12) Ht 37% Kosiborod et al. 2003 (39), McClellan et al. (38) Ht 37% Terrovitis et al. (31) Ht 37% in women and 40% in men Kosiborod et al. 2005 (4) Ht hematocrit; WHO World Health Organization denition of anemia, Hb 12.0 g/dl in women, 13.0 in men; other abbreviations as in Table 2. Continued Table 2 Continued Author (Ref. #), Year Follow-Up n Patients End Points Major Exclusion Criteria Study Design Study Varadarajan et al. (6), 2006 5 yrs 2,246 Primary discharge of diagnosis of CHF Mortality, hospitalization, ED visit CHF excluded with Framingham criteria, no ECG Retrospective, observational, single-center, chart review Good Schou et al. (17), 2007 30 months 345 Systolic HF (LVEF 45%) Mortality Prospective, observational, single-center cohort study Good ACS acute coronary syndromes; AoS aortic valve stenosis; CABG coronary arterial bypass graft surgery; CAD coronary artery disease; CHARM Candesartan in Heart Failure trial; CHF chronic heart failure; COMET Carvedilol Or Metoprolol European Trial; COPD chronic obstructive pulmonary disease; CVA cerebral vascular accident; ECG electrocardiogram; ED emergency department; ELITE II Losartan Heart Failure Survival Study; F/U follow-up; Hb hemoglobin; HF heart failure; HT hypertension; Htc hematocrit; ICD-9-CM International Classication of Diseases, 9th Revision, Clinical Modication; IN-CHF Italian NetworkCongestive Heart Failure trial; IV intravenous; LVEF left ventricular ejection fraction; MI myocardial infarction; MiS mitral valve stenosis; NYHA New York Heart Association functional class; PRAISE Prospective Randomized Amlopidine Survival Evaluation trial; PTCA percutaneous transluminal coronary angioplasty; RENAISSANCE Randomized Etanercept North American Strategy to Study Antagonism of Cytokines; sCR serum creatinine; Tx heart transplantation; Val-HeFT Valsartan Heart Failure Trial. 823 JACC Vol. 52, No. 10, 2008 Groenveld et al. September 2, 2008:81827 Anemia and Mortality in Heart Failure Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 mortality risk caused by renal failure (47). From a patho- physiological standpoint, CHF can also cause renal failure due to a decreased cardiac output reducing renal perfusion (i.e., forward failure); subsequent renal failure may lead to decreased endogenous EPO levels and may ultimately induce anemia, leading to an increased cardiac workload completing the vicious circle. Recently this has been called the cardio-renal-anemia syndrome (48). Findings from intervention studies. Considering the in- creased mortality risk caused by anemia, in heart failure as well as renal failure, trials have been designed in which patients receive erythropoiesis-stimulating proteins (ESPs) to increase Hb levels. The rst intervention study to address the efcacy of EPO in CHF patients was performed by Silverberg et al. (49) in 32 patients. Correction of anemia with EPO and intravenous iron led to a signicant increase in left ventricular ejection fraction and decrease in New York Heart Association functional class, which was re- ected by almost 90% reduction in the number of hospital- Figure 2 Risk of All-Cause Mortality of Anemic Versus Nonanemic CHF Patients CHF chronic heart failure. CI condence interval. Figure 3 Funnel Plot for Publication Bias OR odds ratio. 824 Groenveld et al. JACC Vol. 52, No. 10, 2008 Anemia and Mortality in Heart Failure September 2, 2008:81827 Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 izations. A similar study showed that EPO treatment signicantly increased peak oxygen consumption and exer- cise duration in patients with moderate-to-severe CHF (50). Recently, 2 substantially larger multicenter phase II studies evaluated the effects of darbepoetin (a long-acting ESP) on surrogate cardiovascular end points (51,52). Treat- ment with darbepoetin was safe and effectively raised Hb. Moreover, it signicantly improved clinical status; however, no signicant improvement in exercise tolerance could be observed. However, recently several studies showed a potentially harmful effect of ESP treatment in patients with kidney disease and malignancies. A meta-analysis in patients with cancer-associated anemia showed an increased risk in ve- nous thromboembolism and mortality associated with re- combinant EPO and darbepoetin administration (53). Fur- thermore, concerns about the cardiovascular safety of ESP in patients with kidney disease have been raised. Two separate studies showed that patients targeted to a higher Hb level had an increased incidence of cardiovascular events (54,55). However, in these studies, no placebo groups were included. These studies were all performed in patients with severe renal failure, and only a minority of patients suffered from heart failure. Consequently, the results of the studies mentioned in the preceding text cannot be extrapolated to the CHF population. Larger randomized studies are clearly needed to deter- mine the impact on morbidity and mortality in CHF. Currently, a large phase III morbidity and mortality trial, the RED-HF (Reduction of Events with Darbepoetin alfa in Heart Failure) trial (56), is being conducted to answer the question whether the use of ESPs in anemic heart failure patients is benecial. Besides ESP, intrave- nous iron treatment is another intervention modality in anemic CHF patients. Recently, 2 trials appeared show- Figure 4 Relationship Between Baseline Hemoglobin and Annual Mortality The area of each circle is proportional to the sample size in each cohort. The cen- ter line shows the estimated mortality risk per year of lower baseline Hb values on a continuous scale. The dotted lines represent the 95% condence intervals. Potential Confounders of Included Studies Table 4 Potential Confounders of Included Studies Author (Ref. #) Number of Patients Age Gender Renal Function Severity of Heart Failure Medical History Medication Elabbassi et al. (8) 437 Maraldi et al. (11) 567 Berry et al. (7) 519 Newton and Squire (15) 528 Hebert et al. (25) 410 Felker et al. (13) 4,951 Kosiborod et al. (4) 50,405 Maggioni-V et al. (5) 5,010 Maggioni-I et al. (5) 2,411 van der Meer et al. (30) 74 Szachniewicz et al. (33) 176 Mozaffarian et al. (37) 1,130 Go et al. (3) 59,772 Kalra et al. (26) 531 Schou et al. (17) 345 Denitions of Systolic and Diastolic HF Used in the Original Articles Table 5 Denitions of Systolic and Diastolic HF Used in the Original Articles LVEF (%) Systolic CHF Mozaffarian et al. (37), Anand et al. (1) 30 Komajda et al. (10), Terrovitis et al. (31) 35 Sharma et al. (34), Ralli et al. (16), Maggioni et al. (5), Kerzner et al. (40), Kosiborod et al. (4), OMeara et al. (36), Felker et al. (13) 40 Schou et al. (17) 45 Diastolic CHF Kerzner et al. (40), Kosiborod et al. (4), OMeara et al. (36), Felker et al. (13) 40 Grigorian Shamagian et al. (14) 50 Abbreviations as in Table 2. 825 JACC Vol. 52, No. 10, 2008 Groenveld et al. September 2, 2008:81827 Anemia and Mortality in Heart Failure Downloaded From: http://content.onlinejacc.org/ on 05/07/2014 ing that intravenous iron without ESPs increased Hb levels in patients with CHF. Moreover, they showed an improved exercise capacity and decreased NT-proBNP levels (57,58). Further research is needed to study the effect of intravenous iron on morbidity and mortality in anemic CHF patients. Study limitations. Limitations of meta-analyses include differences in designs and populations used in included trials, quality of studies, and heterogeneity (59). However, the quality of all studies was graded according to published guidelines and all met at least 7 of 12 criteria. Evaluation of anemia was only at baseline in all included studies. Tang et al. (60) recently reported a study with serial hemoglobin measurements and showed that persistent anemia has a larger effect on mortality than transient anemia. No evaluation of Hb was per- formed during the studies; thus, it remains unknown whether anemia in the included studies was persistent or transient. Furthermore, heterogeneity in our study is substantial and can be attributed to differences in dura- tion of follow-up, type of patients included, study design, and denition of anemia. Denition of anemia was a major cause of heterogeneity. When mortality risk was assessed using studies that dened anemia by WHO criteria, heterogeneity was no longer signicant, while the increased mortality risk caused by anemia remained. Furthermore, our meta-analysis could not exclude resid- ual confounding from studies in the adjusted analyses, although most studies adjusted for many known major confounders. However, this limitation emphasizes the inability of our analysis to prove causality. Previously, it has been argued that a larger meta-analysis (e.g., 1,000 events) is clinically more meaningful (59); our study, which includes over 50,000 events, is therefore of particular note. Besides the overall size, the individual studies in the present analysis were of considerable size, with 14 including more than 1,000 patients. These strengths make our ndings all the more rm. Conclusions Anemia is present in one-third of the CHF population and is an independent risk prognosticator for mortality in subjects so affected, irrespective of a systolic versus diastolic etiology of CHF. Further research is needed to assess the effect of correcting anemia in CHF patients. Reprint requests and correspondence: Dr. Peter van der Meer, Cardiovascular Research Center, Massachusetts General Hospital, Charles River Plaza, 185 Cambridge Street, Boston, Massachu- setts 02114. E-mail: pvandermeer@partners.org. 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Evaluation and long-term prognosis of new-onset, transient, and persistent anemia in ambulatory patients with chronic heart failure. J Am Coll Cardiol 2008;51:56976. Key Words: heart failure y anemia y prognosis. APPENDIX For the Acknowledgments and supplementary Tables 1 and 2, please see the online version of this article. 827 JACC Vol. 52, No. 10, 2008 Groenveld et al. September 2, 2008:81827 Anemia and Mortality in Heart Failure Downloaded From: http://content.onlinejacc.org/ on 05/07/2014