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Wizard Genomic DNA Purification Kit Protocol
Wizard Genomic DNA Purification Kit Protocol
Wizard Genomic DNA Purification Kit Protocol
Genomic DNA
Purication Kit
Instrucons for use of Product
A1120 , A1123, A1125 AND A1620
T E C H N I C A L M A N U A L
Revised 12/10
TM050
Promega Corporation 2800 Woods Hollow Road Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 Phone 608-274-4330 Fax 608-277-2516 www.promega.com
Printed in USA. Part# TM050
Revised 12/10 Page 1
1. Description..........................................................................................................1
2. Product Components and Storage Conditions ............................................2
3. Protocols for Genomic DNA Isolation..........................................................5
A. Isolating Genomic DNA from Whole Blood
(300l or 3ml Sample Volume)...........................................................................5
B. Isolating Genomic DNA from Whole Blood
(10ml Sample Volume) ........................................................................................7
C. Isolating Genomic DNA from Whole Blood
(96-Well Plate) .......................................................................................................9
D. Isolating Genomic DNA from Tissue Culture Cells and
Animal Tissue .....................................................................................................11
E. Isolating Genomic DNA from Plant Tissue ...................................................13
F. Isolating Genomic DNA from Yeast................................................................14
G. Isolating Genomic DNA from Gram Positive and
Gram Negative Bacteria ....................................................................................16
4. Troubleshooting...............................................................................................17
5. References .........................................................................................................18
6. Appendix ...........................................................................................................19
A. Composition of Buffers and Solutions ............................................................19
B. Related Products.................................................................................................19
1. Description
The Wizard
Cat.# 3896)
isopropanol, room temperature
70% ethanol, room temperature
96-well plate sealers (Costar