Blood Banking

Centrifugation
Chemistry
Flow Cytometry
Hematology
Hemostasis
Immunoassay
Information Systems
Lab Automation
Molecular Diagnostics
Rapid Diagnostics
BR-14792A
Optimize workflow with a cytometer
that brings more to your lab.
Navios* Flow Cytometer
A flow cytometer is an essential tool for clinical diagnosis and
research and can be used in a wide range of applications, from
immunophenotyping to ploidy analysis and GFP expression analysis. It is
a powerful technique for the analysis of multiple parameters of individual
cells within heterogeneous populations. With Beckman Coulters
Navios Flow Cytometer, you get more innovative features, reliability and
performance than any other flow cytometer available on the market today.
From its advance optical design to its powerful software, you can be
assured you are getting a state-of-the-art, easy-to-use flow cytometer for
your laboratory applications.
Enhanced Scatter Detection
The customized forward scatter allows you to achieve the most accurate size
measurement, while our patented electronics provide accurate digital signal
processing at high event rates over a wide dynamic range of fluorescence
intensities. An innovative forward-scatter detector provides relative cell size
and visualization of particles down to 0.404m in diameter. A side-scatter
detector incorporates an independently focused, high-performance photodiode
with electronic attenuation. Six fluorescence detectors provide simultaneous
acquisition of up to six fluorescence signals. The three scalable configurations
allow for the future addition of more lasers and fluorescence detectors as the
applications for complex multi-color flow cytometry expand.
Navios acquires superior data from multiple colors.
Advanced Optical Design
The Navios was built with an advanced optical system to enhance sensitivity
of multi-color assays. Light on the Navios system is preserved at the flow cell
where the fluorescence pickup lens is gel coupled. This method increases the
light collection efficiency by 77%. The emitted light is then focused and captured
onto fiber optics. These multimode fiber optic cables efficiently deliver the light
to the back of the boulevard. The filters are laid out at an optimal 18 degrees
in order to minimize light loss that typically occurs as light is transmitted
through filters. This allows the Navios to provide exceptional
sensitivity and signal to noise resolution even at the last
filter in the array.
Consumer Reliability
Get automated tracking of your samples with the unique
barcode identification system. From aspiration to reporting,
you have assurance your samples are positively identified.
The barcode on your sample is scanned and confirmed
with the Navios software. Now you can easily verify the
carousel number, carousel position and tube label.
Powerful Easy-to-Use Software
The Navios software reports cellular characteristics such
as size, granularity, phenotype and health. Your results
are combined into one automated, customized report with
plots and formulas. A variety of reporting options
is available to facilitate your workflow.
Easy Automation and Integration
The Navios Flow Cytometer easily integrates with a variety of automated preparation
devices available from Beckman Coulter or devices from other laboratory
manufacturers to make your daily work faster and more efficient. All Navios flow
cytometers are LIS-ready; you get a complete sample in/result out solution for
your clinical laboratory. You can be assured of secure, high-throughput results,
even at the highest volumes.
Easy-to-Use
LEDs on the dashboard clearly indicate instrument status that you can see
from across the lab. If you do need assistance, help is available 24-7. With our
PROService remote diagnostic capabilities youll get highly-qualified service
and support personnel to review your instrumentations status, troubleshoot any
issues, and resolve any problems, so your Navios Flow Cytometer is working at
peak performance at all times. Its our commitment to total customer satisfaction.
Solid State Laser Technology
The Navios offers up to three, fixed solid-state dialed lasers. The lasers are
independently focused in the auto-beam tower using an innovative steering
design mechanism that provides optimal excitation at the flow cell for reliable,
high-quality results. Easily interchangeable optical filters facilitate the detection
of a variety of dyes and wavelengths. Advanced electronics provide accurate
and efficient digital signal processing at high event rates over a wide dynamic
range of fluorescence intensities.
OPTICS
Lasers
LASERS/POWER OUTPUT
Blue Solid State Diode: 488nm, 22mW laser output
Red Solid State Diode: 638nm, 25mW laser output
Violet Solid State Diode: 405nm, 40mW laser output**
CONFIGURATION
125m spatially separated beam spots
MINIMUM LASER POWER AT FLOW CELL
Blue: > 20mW
Red: > 20mW
Violet: > 30mW**
Flow Cell
150 x 460m rectangular quartz
Collection Optics
Gel coupled 1.2 NA lens
Optical Filters
Easily interchangeable optical filters
Optimal 18-degree reflective optics for minimal light loss
Detector Filters
Forward Scatter: 488/10
Blue Laser: 525/40, 575/30, 620/30, 675/20**, 695/30, 755LP
Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7
Red Laser: 660/20, 725/20*, 755 LP*
Dyes: APC or Alexa Fluor

647, APCAlexa Fluor 700, APCCy7,

APCAlexa Fluor750
Violet Laser:** 450/50, 550/40
Dyes: Pacific Blue

, Pacific Orange

, Krome Orange
Detectors
FORWARD SCATTER DETECTOR
Fourier design providing up to 3 measurements of forward angle
SIDE SCATTER DETECTOR
Independently focused high performance photodiode
with electronic attenuation
FLUORESCENCE DETECTORS
FL1 FL10 Fluorescent Detectors (710 optional**)
SAMPLE PROCESSING
Flow Rates
Continuous pressure is applied to the sample tube
based on user selected flow rates:
Low, Medium and High
Sheath Consumption
Acquisition: 780mL/hour
Carryover: < 0.1%
Compatibility: 12 x 75mm tubes
Acquisition Modes
32 tube Multi Carousel Loader (MCL)
Single tube sampling mode
Automated work list acquisition
Manual work list mode
Mixing
The MCL patented design vortexes each tube individually
before sample acquisition
Barcode Reading
Carousel number, tube location and tube barcode
Biosafety
Biohazard contained wash station thoroughly rinses sample probe
Fluidics
10L IsoFlow External Sheath Container
20L Waste Container
1.5L FlowClean Cleaning Fluid Tank
1.5L Internal Sheath Tank
SIGNAL PROCESSING
Flow Rates
Dynamic Range: 20-bit data acquisition
Workstation Resolution: 1,048,576 channels
Digital Sampling Rate: 40MHz
Digital Accuracy: < 5% error
Parameters:
Five different signals available from each detector:
Integral linear and logarithmic, Peak linear
and logarithmic and True Time of Flight linear
Time, Ratio
Selection of up to 62 parameters
Navios Specifications and Performance Characteristics
For Beckman Coulters worldwide office locations and phone numbers, please visit Contact Us at www.beckmancoulter.com
B2010-12033-DG-2500 2011 Beckman Coulter, Inc. PRINTED IN U.S.A.
CE Marked
* Pending clearance by the U.S. FDA. Not yet available in the U.S. for in vitro diagnostic use.
** Optionally available depending on upgraded system conguration
*** Optional lter included
Alexa Fluor, Pacic Blue, and Pacic Orange are registered trademarks of Molecular Probes, Inc.
Pentium Core is a trademark of the Intel Corporation.
These characteristics can be inuenced by a number of factors relating to instrument setup, sample type,
number of parameters selected, protocol denition and number of events acquired. Refer to Instrument Instructions
for User for more information on Performance Characteristics.
Beckman Coulter and the stylized logo are registered trademarks of Beckman Coulter, Inc.
PERFORMANCE CHARACTERISTICS

Throughput
Throughput of 10k normal lymphocytes is 80 tubes/hour
Up to 88 tubes an hour at 10,000 events per second
Scatter Resolution
Resolves 0.404m diameter particles from background
noise using forward scatter with maximum detection
up to 40m diameter particles
Fluorescence Sensitivity Threshold Levels
FITC 112 MESF PE 78 MESF
PECy5 15 MESF APC 75 MESF
Acquisition Rate
25,000 events per second
REMOTE DIAGNOSTICS
PROService
PROService compatible; high-speed Internet connectivity
with optional hardware for remote system monitoring,
diagnostics and repair
WORKSTATION (MINIMUM SPECIFICATIONS)
Operating System: Windows Vista Business
RAM: 4GB
Processor Frequency: Pentium Core

2 Duo 2.13GHz
Hard Drive: 160GB
Removable Media Support: DVD 18X, CD 40X
Network Ports: 3, 2 available for networking
Video Card: PCI express x 16, 256MB DDR2
64-bit onboard memory
Support for 1680 x 1050 resolution dual monitors
USB Ports: 8
RoHS Compliant
Monitor: 22-inch Flat Panel LCD Monitor
INSTALLATION REQUIREMENTS
Power: Universal Power Supply (100-240 VAC, 50-60Hz)
Operating Temperature: 16 32C (60-90F)
Noise: 60db
PHYSICAL DIMENSIONS:
ORDERING INFORMATION
Part Number/Description
A80704 6 colors, 2 lasers (5+1 configuration)
A80705 8 colors, 2 lasers (5+3 configuration)
A80706 10 colors, 3 lasers (5+3+2 configuration)
Cytometer
Weight 104kg 230lbs
Width 96cm 38in
Height 61cm 24in
Depth 70cm 28in
Supply Cart
Weight 30kg 67lbs
Width 72.4cm 28.5in
Height 29.8cm 11.75in
Depth 49.5cm 19.5in
For more information about the Navios Flow Cytometer, contact your local
Beckman Coulter office or log on to our website at: www.coulterflow.com