Amnesic shellsh poisoning (ASP) causing toxins are

produced mainly by the toxigenic diatom strain Pseudo-

nitzchia multiseries. The ASP toxins include primarily domoic
acid (DA) and associated compounds such as epi-domoic acid.
In addition to contamination of seafood, these marine biotoxins
can result in human and marine wildlife mortality. The clinical
toxicological effects attributed to DA can include: permanent
loss of short-term memory, nausea, vomiting, headache,
disorientation, and loss of balance.
Intended Use / User
Reveal

2.0 for ASP is intended for the qualitative screening

of shellsh for DA, with the cut off value for a positive result
at 20 ppm. The test kit is designed for use by personnel with
an interest in the rapid screening of shellsh samples, such as
harvesters and processors.
Designed to test:
Mussels, Scallops, Oysters, Clams, Cockles
Why use Reveal

2.0 for ASP?

Ease of use
Single step rapid test
10 minutes to result
Usable in the feld as well as in the lab
Compatible with FDA and EU Commission permitted levels
Consistent interpretation of results using AccuScan

Pro
Reader
Backed by Neogens experienced customer and technical
support service
Introducing the latest addition to the Reveal 2.0 range
AccuScan

Pro Reader
AccuScan Pro removes subjectivity from reading the
lateral ow device and provides consistent interpretation
and the permanent records to back it up.
To place an order, or for more information, contact your local Neogen Europe
representative, or visit our web site at www.neogeneurope.com
Neogen Europe Ltd. The Dairy School Auchincruive, Ayr, Scotland KA6 5HW
Tel: +44 (0)1292 525610 Fax: +44 (0)1292 525602
www.neogeneurope.com info_uk@neogeneurope.com
Neogen Corporation, 2013. Neogen and Reveal are registered trademarks of Neogen Corporation, Lansing, Michigan. NE1655-0113
8. Transfer 100 L of the diluted
sample to a microwell.
Reveal

2.0 for ASP

Procedure
4. Fold the upper edge of the bag
over the green straw and clip the
white clip to prevent leakage of the
sample.
5. Press the roller frmly on the
sample extraction bag, pushing
the roller back and forth for 30
seconds to aid in obtaining a
homogenous sample extract.
6. Pour all bag contents from side
2 back into original distilled
water container. Discard the used
extraction bag.
7. Remove 100 L of the sample
extract using a disposable pipettor,
add into ASP buffer vial and mix.
1. Weigh out 1 g ( 0.1 g) of
homogenised sample and 30 mL
distilled water. Shake cup for 30
seconds.
2. Number both sides of the
extraction bag, one side is labelled
1 and opposite side labelled 2.
3. Pour solution/sample mixture to
bottom of the extraction bag on
side 1
Note: Please read kit instructions completely before performing test.
10. Remove promptly at 10 minutes
and interpret results using the
AccuScan

Pro Reader.
9. Place the ASP strip into the well.
Set a timer for 10 minutes.
Ordering Info
9560 Reveal 2.0 for ASP, 24 tests per kit
9563 Marine Biotoxins Starter Kit
9565 AccuScan Pro Reader
To place an order, or for more information, contact your local Neogen Europe
representative, or visit our web site at www.neogeneurope.com
Neogen Europe Ltd. The Dairy School Auchincruive, Ayr, Scotland KA6 5HW
Tel: +44 (0)1292 525610 Fax: +44 (0)1292 525602
www.neogeneurope.com info_uk@neogeneurope.com
Neogen Corporation, 2013. Neogen and Reveal are registered trademarks of Neogen Corporation, Lansing, Michigan. NE1655-0113