Maria del Mar Martinez de Altube

Maria del Mar Martinez de Altube

& Alejandro Martinez Pe
& Alejandro Martinez Pe

a
a
Control of the flatheaded rootborer
Control of the flatheaded rootborer
(
(
Capnodis tenebrionis
Capnodis tenebrionis
) with the
) with the
entomopathogenic nematode
entomopathogenic nematode
Steinernema carpocapsae
Steinernema carpocapsae
MEDITERRANEAN FLATHEADED ROOTBORER
Capnodis tenebrionis
(Coleoptera: Buprestidae)
CHERRY
APRICOT
NECTARINE
PEACH
ALMOND
PLUM
APPLE
PEAR
IN
OFF
FLATHEADED ROOTBORER PEST
FLATHEADED ROOTBORER PEST
24.000 ha
21.000 ha
33.000 ha
70.000 ha
APRICOT
PLUM
CHERRY
PEACH + NECTARINE
CURRENT SITUATION SPAIN
Capnodis
Capnodis
tenebrionis
tenebrionis
Larvae
Egg
Neonate larvae
Adult
A A A A A A A A P P P P L L L L L L L L L L L L 2 YEAR 2 YEAR
L L L L L L L L L L L L E E A A A A A A A A A A 1 YEAR 1 YEAR
D D N N O O S S A A J J J J M M A A M M F F J J
Pupae
PEST SYMPTOMS
PEST SYMPTOMS
RESIN ON THE TRUNK
LACK OF NEW SHOOTS
GALLERIES AND HOLES IN THE
TRUNK
CHITOSAN + EPN
N-acetyl-glucosamine
+
PEST CONTROL
PLANT RECOVERY
EPN
Activates plant defence
Promotes root development
Objective
Objective
field
field
tests
tests
Determine
Determine
effect
effect
of
of
Biorend
Biorend
R
R
against
against
Capnodis
Capnodis
tenebrionis
tenebrionis
in
in
apricot
apricot
(
(
Prunus
Prunus
armeniaca
armeniaca
)
)
We
We
tested
tested
the
the
effect
effect
of
of
1.
1.
Dose
Dose
2.
2.
Application
Application
technique
technique
3. Time
3. Time
for
for
application
application
1. DOSES TESTED
1. DOSES TESTED

1=
1= 1 1 Million Million EPN + 0,6 g EPN + 0,6 g chitosan chitosan/ / tree tree. .

1,5=
1,5= 1.5 1.5 Million Million EPN + 0,9 g EPN + 0,9 g chitosan chitosan/ / tree tree. .
DOSES DOSES
Nematodes Nematodes- -chitosan chitosan/ / tree tree
(S.carpocapsae, SSA3)
2. APPLICATIONS TESTED
2. APPLICATIONS TESTED

1.
1.
Drench
Drench
. (
. (
Water
Water
around
around
the
the
tree
tree
).
).

2.
2.
Injection
Injection
around
around
the
the
tree
tree

3.
3.
Drip
Drip
irrigation
irrigation
DRENCH
DRENCH
INJECTION APPLICATION
INJECTION APPLICATION
Injector
Injector
DRIP APPLICATION
DRIP APPLICATION
1
1
-
-
4
4
drip
drip
holes
holes
around
around
the
the
tree
tree
.
.
18l
18l
per
per
tree
tree
/
/
hour
hour
in
in
summer
summer
2h/
2h/
day
day
36l/
36l/
tree
tree
+
+
Solution
Solution
(
(
nematodes+chitosan
nematodes+chitosan
)
)
0
5
10
15
20
25
30
CONTROL D+INJ DRIP INJ 1 INJ 2
M
e
a
n

n
u
m
b
e
r

i
n
s
e
c
t
s

/

t
r
e
e
0
10
20
30
40
50
60
70
80
90
100
A
b
b
o
t
t

c
o
r
r
e
c
t
e
d

m
o
r
t
a
l
i
t
y

(
%
)
alive nematode infected efficacy
F= 24.68, df= 4, 17, P= 0.05
12
24.5
92%
94%
90%
89%
2
1.3
3.3
2.4
12
2.7
10
14
4.6
14.4
12.7
24.5
Control
Control
of
of
C.tenebrionis
C.tenebrionis
with
with
Biorend
Biorend
R
R
0
5
10
15
20
25
30
CONTROL D INJ 1 INJ 2
M
e
a
n

n
u
m
b
e
r

i
n
s
e
c
t
s

/

t
r
e
e
0
10
20
30
40
50
60
70
80
90
100
A
b
b
o
t

c
a
r
r
e
s
c
t
e
d

m
o
r
t
a
l
i
t
y
(
%
)
alive nematode infected efficacy
F = 11.65, df= 3, 8, P= 0.0027
25.6
25.6
8.3
3.3
5
80%
9.2
5.6
3.6
78%
3.3
4
84%
7.3
Control
Control
of
of
C.tenebrionis
C.tenebrionis
with
with
Biorend
Biorend
R
R
EFFECTS
After 1 year
EFFECTS
After 1 year
Conclusion
Conclusion

In 5
In 5
field
field
trials
trials
control
control
surpassed
surpassed
80%
80%

No
No
impact
impact
of dose
of dose
or
or
application
application
technique
technique

Application
Application
best in
best in
autumn
autumn
+ spring
+ spring

Biorend
Biorend
R
R
should
should
be
be
used
used
for
for
3
3
years
years

EPN
EPN
detected
detected
in
in
soil
soil
until
until
end of
end of
trial
trial
Conclusion
Conclusion

Biorend
Biorend
R
R
give
give
excellent
excellent
control
control

Rentability
Rentability
of
of
orchards
orchards
maintained
maintained