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Rapid Prototyping Ufluidic Cart Eletrchem
Rapid Prototyping Ufluidic Cart Eletrchem
C for 90min.
This annealingstepwas includedtoavoidstress cracks intheacrylic
[34].
Once all the layers are ready, assembly began with bonding of
the bottomPET lm(L1) and the rst PSA (L2). In the rst step, cut-
out regions (i.e., channels and reservoirs) of the PSAwere peeled off
from the bottom release liner. An application tape, typically one-
sided adhesive backed lm, was applied to the peeled-off side and
squeezed to hold the pattern in place. Then the release liner was
carefully detached, leaving the engraved pattern on the applica-
tion tape pattern transfer [22]. The application tape bearing the
pattern (L2) was placed on a custom-made aligner with 4 press-
tted dowel pins. Each layer has 4 alignment holes at the corners,
and each hole matches the corresponding dowel pin in its position
for accurate alignment. The PET lm (L1) incorporating immobi-
lized antibody was adhesively bonded to the PSA (L2) held on the
aligner using a hand roller. To integrate the acrylic layer (L3) with
the PET-PSA assembly (L1/L2), the assembly was put back on the
aligner withits adhesive side facing upfollowing the removal of the
top release liner along with the application tape. The acrylic sheet
was mated with the assembly held on the aligner to form a PET-
PSA-acrylic assembly (L1/L2/L3). A laser-cut absorbent pad (M3,
790mthick, pure cellulose bers, 133, Pall) was attached to an
adhesive region of the assembly (L1/L2/L3). Next, a pattern trans-
ferred fromthe second PSA (L4) was bonded to the PET-PSA-acrylic
assembly (L1/L2/L3) in the same way as described above (i.e., bond-
ingof L1andL2). The layer-by-layer assemblyprocess was repeated
Fig. 1. Fabrication process of a multilayered, polymeric microuidic cartridge. (A) Exploded view of all patterned layers. Alternating layers of thin plastic lm/sheet (blue)
and double-sided tape (yellow). (B) Schematic cross-section of all layers in the stack. Liquid is drawn into an absorbent-embedded waste reservoir by vacuum when the
tape is pierced. (C) A photograph (top view) of a 3-D microuidic cartridge (85mm54mm1.8mm) constructed by layer-by-layer assembly. M1, M2 and M3 are the rst,
second and third membrane, respectively. C, R, and Wrepresent the counter, reference and working electrode, respectively. The sense electrodes monitor the position of a
liquid meniscus.
62 J. Kimet al. / Sensors and Actuators B 202 (2014) 6066
Table 1
Description of each layer regarding material, thickness, function, and patterning
tool. DST represents the double-sided tape.
Material Thickness (m) Function Pattering tool
Layer 7 PET 125 Lid Cutting plotter
Layer 6 DST 130 Channel Cutting plotter
Layer 5 PET 125 Support Cutting plotter
Layer 4 DST 130 Via hole Cutting plotter
Layer 3 Acrylic 1000 Reservoir CO
2
laser
Layer 2 DST 130 Channel Cutting plotter
Layer 1 PET 125 Electrode Cutting plotter
until the top PET layer (L7) was integrated into a whole assembly
(L1 through L7) as described in Fig. 1B. The whole assembly was
then compressed multiple times using a desktop cold laminator
to ensure sufcient bonding strength as well as a tight seal of the
integrated Au electrodes. Table 1 shows the summary of detailed
information on each layer.
2.2. Integration of porous membranes
Tween-20 detergent is routinely added to phosphate-buffered
saline (PBS) or tris-buffered saline (TBS) wash buffer to minimize
the background noise from non-specic binding in bioassays. To
simplifytheon-chipassayprocedure, weembeddedaporous mem-
brane containing dried Tween-20 in a CEB. In this way, we could
minimize the complexity of device fabrication by eliminating the
need for liquid buffer storage in the CEB. At rst, a porous mem-
brane (419mthick, spun bonded polyester, 6613, Pall) was cut
into 3-mm and 5-mm circles. The 3-mm disk membranes were
immersed in a tube containing 50% Tween-20 (P9416, Sigma) for
1h. The wetted membranes were removed fromthe tube and dried
in a 50