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Naruszewicz 2008
Naruszewicz 2008
Oxidative stress
Oxidative stress
Anti#inflammatory
Anti#inflammatory
#ranscription #ranscription
Cholesterol esters Cholesterol esters
OX-LDL OX-LDL
LDL LDL
s-IC!M s-IC!M
s-$C!M s-$C!M
LOX- LOX-
LOX- LOX-
Aronia )erries
Aronia )erries
Aronia *("otinia melanocaarpa+ also known as Aronia melanocarpa and Aronia *("otinia melanocaarpa+ also known as Aronia melanocarpa and
Aronia nira,+ is a native American $us" t"at "as $een successfully Aronia nira,+ is a native American $us" t"at "as $een successfully
exported to Eastern Europe and is commercially rown in 'enmark+ exported to Eastern Europe and is commercially rown in 'enmark+
(oland+ %ussia and elsew"ere. (oland+ %ussia and elsew"ere.
-nown commonly as .c"oke$erry/ or .$lak c"oke$erry/+ *("otinia -nown commonly as .c"oke$erry/ or .$lak c"oke$erry/+ *("otinia
melanocarpa, "as at least two cousins wort" mentionin. 0"ese are melanocarpa, "as at least two cousins wort" mentionin. 0"ese are
("otinia flori$unda+ also known as Aronia ("otinia flori$unda+ also known as Aronia a atropurpurea+ t"e .purple tropurpurea+ t"e .purple
c"oke$erry/+ and ("otinia pyrofilia+ also known as Aronia ar$utifolia+ c"oke$erry/+ and ("otinia pyrofilia+ also known as Aronia ar$utifolia+
t"e .red c"oke$erry/. 0"e former ranes from Mississippi to Wisconsin+ t"e .red c"oke$erry/. 0"e former ranes from Mississippi to Wisconsin+
1eoria into !anada+ and all areas in $etween. 0"e latter ranes from 1eoria into !anada+ and all areas in $etween. 0"e latter ranes from
0exas#2kla"oma eastward t"rou" 0ennessee to t"e Atlantic and from 0exas#2kla"oma eastward t"rou" 0ennessee to t"e Atlantic and from
3lorida nort"ward into !anada. 0"e Aronia $erry "as all of t"e "ealt"y 3lorida nort"ward into !anada. 0"e Aronia $erry "as all of t"e "ealt"y
attri$utes of t"e cran$erry+ $ut also contains five to ten times t"e attri$utes of t"e cran$erry+ $ut also contains five to ten times t"e
amount of ant"ocyanins and polyp"enols of a cran$erry. amount of ant"ocyanins and polyp"enols of a cran$erry.
Antioxidant activity *2%A!,+ Ant"ocyanin
Antioxidant activity *2%A!,+ Ant"ocyanin
!ontent and ("enolic !ontent in 3ruit of
!ontent and ("enolic !ontent in 3ruit of
)lue$erry+ !ran$erry+ &inon$erry and
)lue$erry+ !ran$erry+ &inon$erry and
!"oke$erry
!"oke$erry
W. Zheng, S.Y. Wang Oxygen radical absorbing capacity of phenolics in blueberries, cranberries,
chokeberries and lingonberries, J.gric.!ood "he#. $%, &'(()* $(%+$(,
25.56 .2! "6#.2 $hokeberry
4.56 7.85 9:.; &inon$erry
9.;5 7.96 ;:.5 !ran$erry
8.;6 ;.67 6:.< )lue$erry
0otal p"enolic
*m=,
Ant"ocyanin
*m=,
2%A!
*>mol of 0E=,
species
Effect of
Effect of
A. melanocarpa
A. melanocarpa
extract on superoxide
extract on superoxide
production $y "uman arterial wall cells from patients
production $y "uman arterial wall cells from patients
wit" cardiovascular risk factors
wit" cardiovascular risk factors
NA'(? oxidase activity NA'(? oxidase activity %2S eneration %2S eneration
kontrola 0,1 1 5 10 100 kontrola 0,1 1 5 10 100
0 0
00 00
!0 !0
0 0
1"00 1"00
160 160
0 0
"000 "000
"00 "00
"!00 "!00
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kontrola 0,1 1 5 10 100 kontrola 0,1 1 5 10 100
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concentration of concentration of A. melanocarpa A. melanocarpa
pol,phenols e-tract & pol,phenols e-tract & *)ml+ *)ml+
concetration of concetration of A. melanocarpa A. melanocarpa
pol,phenols e-tract & pol,phenols e-tract &. .*)ml+ *)ml+
'ata are s"own as means @=#SEM+ A # pB7+75 vs. native usin appropriate tests dependin on data distri$ution
Effect of
Effect of
A. melanocarpa
A. melanocarpa
extract
extract
on
on
vasoconstruction
vasoconstruction
7 7
;7 ;7
67 67
97 97
87 87
57 57
47 47
C7 C7
:7 :7
7 7 7+77; 7+77; 7+; 7+; 5 5 ;77 ;77
st%&enie polifenoli ekstraktu st%&enie polifenoli ekstraktu '. melanocarpa '. melanocarpa
( () )*+ml, *+ml,
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Increasin concentration of Increasin concentration of
A. melanocarpa extract A. melanocarpa extract
Increasin concentration of Increasin concentration of
A. melanocarpa A. melanocarpa extract extract
!orrelation $etween endot"elium function and
!orrelation $etween endot"elium function and
vasorelaxat
vasorelaxat
ion
ion
activity of
activity of
A. melanocarpa
A. melanocarpa
extract
extract
% % % % 2% 2% 3% 3% &% &% '% '%
/Ch maks% &0+ /Ch maks% &0+
% %
2% 2%
&% &%
6% 6%
(% (%
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M
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ec"anism of vasorelaxtion activity of
ec"anism of vasorelaxtion activity of
A.
A.
melanocarpa
melanocarpa
polyp"enols
polyp"enols
%ole of eN2S
%ole of eN2S
*incu$ation wit" &#NAME,
*incu$ation wit" &#NAME,
Endot"elium function
Endot"elium function
concentration of concentration of A. melanocarpa A. melanocarpa
polyp"enols extract polyp"enols extract * *> >=ml, =ml,
7 7
67 67
87 87
47 47
:7 :7
7 7 7+77; 7+; 5 ;77 7+77; 7+; 5 ;77
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incu$ation wit" &#NAME and polyp"enols incu$ation wit" &#NAME and polyp"enols
incu$ation wit" &#NAME *;77 incu$ation wit" &#NAME *;77 > >M, M,
A A
A A
A A
7 7
67 67
87 87
47 47
:7 :7
7 7 7+77; 7+; 5 ;77 7+77; 7+; 5 ;77
concentration of concentration of A. melanocarpa A. melanocarpa
polyp"enols extract *>=ml, polyp"enols extract *>=ml,
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endot"elial cells endot"elial cells
wit"out wit"out
endot"elial cells endot"elial cells
A A
A A
A A
A A
Superoxide production in platelets and
Superoxide production in platelets and
effect of
effect of
A. melanocarpa
A. melanocarpa
polyp"enols
polyp"enols
(roduction of 2
(roduction of 2
6 6
#. #.
in platelets
in platelets
# correlation wit" risk factors correlation wit" risk factors
control risk factors control risk factors
7 7
57 57
;77 ;77
;57 ;57
677 677
657 657
977 977
957 957
877 877
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Superoxide production in platelets and effect
Superoxide production in platelets and effect
of
of
A. melanocarpa
A. melanocarpa
polyp"enols
polyp"enols
Risk factors
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Effect of increasin
Effect of increasin
A. melanocarpa
A. melanocarpa
polyp"enols concentrations on platelet
polyp"enols concentrations on platelet
areation
areation
Control Control
"is- .actors "is- .actors
% % 2% 2% &% &% 6% 6% (% (% %% %% 2% 2% &% &% 6% 6% (% (% 2%% 2%% 22% 22%
aggregation aggregation /"01 /"01
thro23in ind4ced thro23in ind4ced /2%2052l1 /2%2052l1
CON#"OL CON#"OL
3 3
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2% 2%
3% 3%
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%% %%
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% % 2% 2% &% &% 6% 6% (% (% %% %% 2% 2% &% &% 6% 6% (% (% 2%% 2%% 22% 22%
aggregation aggregation /"01 /"01
thro23in ind4ced thro23in ind4ced /2%2052l1 /2%2052l1
CON#"OL CON#"OL
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0"e effect of increasin concentrations of
0"e effect of increasin concentrations of
Aronia
Aronia
flavonoids from c"oke$erry on
flavonoids from c"oke$erry on
eN2S expression in ?UDE! cells
eN2S expression in ?UDE! cells
0
0,2
0,4
0,6
0,8
1
control 0,1 ug/ml 1 ug/ml
3 hours
24 hours
e
-
O
S
+
%
.
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S
+
%
.
/ /
+
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0"e effect of increasin concentrations of
0"e effect of increasin concentrations of
Aronia
Aronia
flavonoids from c"oke$erry on
flavonoids from c"oke$erry on
&2G#; expression in ?UDE! cells
&2G#; expression in ?UDE! cells
0
0,1
0,2
0,3
0,4
control 0,1 ug/ml 1 ug/ml
3 hours
24 hours
0
O
1
+
%
.
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+
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(roductH A
(roductH A
ronia fruits extract =A%2N2G=
ronia fruits extract =A%2N2G=
Scientific nameH Aronia meelanocarpa
Scientific nameH Aronia meelanocarpa
*c"oke$erry,
*c"oke$erry,
Parameter Result
Aearance 2o3der
Colour 4ed+bro3n
!umi"it# #ax 56
Anthoc#anins
C#$3$%$&a
'(+'$6
Polimeric roc#ani"ins
'()'$) eicatechin
7$$6
Phenolic aci"s ,6
Antio*i"ant acti+it#
' , -rolo*/g "rie" .eight)
/PP! ra"icals
%%5.8
A0-1 ra"icals %(9.%
/ethods
/ethods
388 388
0"e effect of
0"e effect of
Aronia
Aronia
flavonoids on
flavonoids on
plasma 2G#&'& level
plasma 2G#&'& level
0
20
40
60
80
100
120
3efore after
Aronia
Place3o
670001 670001
49/l5
2:8 2:8
0"e effect of Aronia flavonoids on
0"e effect of Aronia flavonoids on
systolic $lood pressure
systolic $lood pressure
118
120
122
124
126
128
130
132
134
136
3efore after
Aronia
Place3o
670001 670001
4mm!g5
* ; 11 mm!g * ; 11 mm!g
0"e effect of Aronia flavonoids on
0"e effect of Aronia flavonoids on
diastolic $lood pressure
diastolic $lood pressure
<4
<6
<8
80
82
84
86
88
3efore after
Aronia
Place3o
4mm!g5
670000 670000
308 308
3
3,2
3,4
3,6
3,8
4
4,2
4,4
4,6
4,8
2
3efore after
Aronia
Place3o
0"e effect of
0"e effect of
Aronia
Aronia
flavonoids on
flavonoids on
plasma "s#!%( level
plasma "s#!%( level
67001 67001
4mg/l5
238 238
0"e effect of Aronia flavonoids on
0"e effect of Aronia flavonoids on
plasma adiponectin level
plasma adiponectin level
3,6
3,8
4
4,2
4,4
4,6
4,8
2
3efore after
Aronia
Place3o
4g/ml5
6703 6703
23768 23768
0"e effect of Aronia flavonoids on
0"e effect of Aronia flavonoids on
plasma M!(#; level
plasma M!(#; level
220
240
260
280
300
320
340
3efore after
Aronia
Place3o
4g/ml5
67001 67001
2:8 2:8
%ole of E(! in reendot"elisation and neovascularisation
%ole of E(! in reendot"elisation and neovascularisation
%eulation of E(!s followin t"e natural "istory of
%eulation of E(!s followin t"e natural "istory of
at"erosclerosis
at"erosclerosis
1. (. 3adini et al.+ At"erosclerosis ;<8 *677C, 84#58. 1. (. 3adini et al.+ At"erosclerosis ;<8 *677C, 84#58.
Endot"elial proenitor cells and at"erosclerosis
Endot"elial proenitor cells and at"erosclerosis