211 5 23

7 28 BAIC4 B17 6
7 1985 6 L mail 7 huocuiying823 C 163 . com
7 L mail 7 pangcl C cau . cdu . cn


1

1

1

1

2

1

1
1 . 183 S 2 .
148 6
7 .
. CA4 CA19 CA53 6 iPB 332 !g L
1
. 857
!g L
1
1 . 47 !g L
1
. 271 !g L
1
. 194 !g L
1
S 8
3 4 2 3 4 2 737 . 5 !g L
1
177 . 5 !g L
1



S216 . 4 7 A 71 1166 211 6 5 7 4
nalysis of Phytohormone and Qunolin-ketone Components in naerobic Digestion Effluent / HUO Cui-ying
1

WU Shu-biao
1
GUO Jian-bin
1
CHENG Hui-cai
1
REN Hong
2
DONG Ren-jie
1
PNG Chang-le
1
/ Q1. Energy
Engineering and Low Carbon Technology Laboratory China gricultural University Beijing 183 Chinap 2.
Beijing Key Lab of Plant Resource Research and Development Food Institute Beijing Technology and Business
University Beijing 148 ChinaI
bstractS Plani hormonc- and quinolinc- componcni- in anacrohic digc-iion cfflucni of pig manurc wcrc quaniiiaiivcly ana
lyzcd wiih HLPC HS and ^HB -pcciro-copy mcihod- cic . 1hc rc-uli -howcd ihrcc kind- of phyiohormonc cxi-iing in ihc
anacrohic digc-iion cfflucni including hcicroauxin gihhcrcllin- CA4 CA19 CA53 6 cyiokinin iPB 6 wiih conccniraiion of
332!g L
1
. 857!g L
1
1 . 47!g L
1
. 271!g L
1
. 194!g L
1
rc-pcciivcly . And iwo kind- of qunolin
kcionc compound- wcrc al-o idcniificd cxi-iing in ihc anacrohic digc-iion cfflucni which wcrc 8hydroxy3 4dihydro2kcionc
and 3 4dihydro2kcionc- and ihc conccniraiion wcrc 737. 5 !gL
1
and 177. 5 !gL
1
rc-pcciivcly. 1hi- rc-uli ihcorciically
cxplaincd di-ca-c rc-i-iancc and growih promoiing cfficicncy of anacrohic digc-iion cfflucni in agriculiurc produciion.
Key wordsS anacrohic digc-iion cfflucni S componcni analy-i- S phyiohormonc S qunolin kcionc
.
28
15625
358 . 26 m
3

24 8 . 56 % 28
26 . 82 %
1
.

.




. .
. .
.
\C 6

2 ~ 8

.





IAA 6 CA- 6
7 !"#$% &#(%) 211 29 5 6
C1- 6 ABA 6
S
. .


1
1. 1

1
1

2
1

/ % . 73
/ % . 34
/ mg L
1
5145 .
/ mg L
1
1291 . 51
/ mg L
1
65 . 35
/ mg L
1
121 . 83
2

LC Icca AHX 1hcrmo Iinnigan
Brukcr A\ 3
API 3 LSI HS AB
Agilcni 11
C
18
HCX cariridgc- Vaicr-
Pharmadcx LH 2
1LC 1 4!m
1. 2
1 . 2 . 1
9 ~ 1
1 mL
2
H
2
CA-
2
H
2

C1-
13
C
6
IAA
2
H
3
ABA
pH 3
pH 8 . 5 6
C
18

6


1 % 89 . 9 % . 1 %
1 % 9 min S . 165
mL min
1
S HS LSI HS
HS HS Xcalihur 2. 1
1 . 2 . 2
4 L

24 146 . 7 g
1 1 6
6 . 5 cm 25 cm 6

6 1 ~ 5
6
A S A pharmadcx LH 2
3 . 5 cm 65 cm 6
1 1 6 5 mL 1
1 2 6

B S B 25 1 6 CI
254 254 nm 6
5 5
9
7 C
1
~ C
9
C
2
C
5

C
2
C
5
1 1 6

I
1
7 . 1 mg 6 I
2
29 . 5 mg 6
1 6 I
1
I
2

1 mL . 22 !m
Agilcni Zorhax SB C18 4 . 6
25 mm 5 !m S 254 nm 28 nm S
7 + 2 % = 5 %
95 % 1 % 7 min S 5
!L S 3 C S 1 . mL min
1
2 6 I
1
I
2
API 3 LSI HS
36 I
1
I
2
IHS0 d
6

2
2. 1

3 CA
19
CA
4
CA
53
iPB IAA
3
8 !"#$% &#(%) 211 29 5 6
3 !g L
1
6
IAA iPB
CA
4
. 857 332 . 194 ^I
CA
19
1 . 471
CA
53
. 271
CA- 6 .

14 CA-

1 ~ 1 ng g
1

.
CA
4
CA
1

CA
2
CA
3

11 ~ 12

Auxin 6
. .
.
1 ~ 1 ng
332 !g L
1

3 ~ 3

13


5
C1- 6
.

iPB

2. 2
I
1
I
2

S I
1

m / z7 H + H
+
164 . 3 m / z7 2 H +
H
+
326 . 5 m / z7 H h H
+
162 . 1
163 I
2
m / z7 H + H
+
148. 2 m / z7 H + ^a
+
17. 1 I
2

147 1 I
1
2 I
2

I
1

1
H ^HB 3 HHz IHS0 d
6
6
9 . 6 8 . 7
6 . 6
2 . 4 2 . 8

13
C ^HB 3 HHz IHS0 d
6
6 ILP1
3 HHz IHS0 d
6
6 1
9 6 3 2
I
2

1
H ^HB 3 HHz IHS0 d
6
6
1 .
6 . 9
2 . 4 2 . 8

13
C ^HB 3 HHz IHS0 d
6
6 ILP1
3 HHz IHS0 d
6
6 2 9
6 4 2
I
1

9 !"#$% &#(%) 211 29 5 6
3 4 2 3 6 4 L
29 . 5 mg . 7375 mg L
1
S
I
2
3 4
2 4 6 4 L
7 . 1 mg L
1
. 1775 mg L
1

.
.

14

pH ^H
+
4

15 ~ 16




14


. .
.

. .




17

3
.

332 !g L
1

CA
4
. 857 !g L
1
CA
19
1 . 47!g L
1

CA
53
. 271!g L
1
iPB . 194
!g L
1

8 3 4 2 3 4
2 737. 5!g L
1
177. 5!g L
1

.



.
.
.



.


1 .
| .
21 28 1 6 721 24 .
2 . | .
25 23 3 6 748 49 .
3 .
| . 27 3 6 79 91 .
4 .
| . 27 5 6 74 43 .
5 .
| . 22 33 2 6 7
13 16 .
6 .
| . 26 24 2 6 751 52 .
7 .
| . 26 22 6 6 7157 16 .
8 |oihi C Pugalcndhi S Poornima ci al . Hanagcmcni
of rooi knoi ncmaiodc in iomaio Lycopcr-icon c-culcnium
Hill wiih hioga- -lurry | . Biorc-ourcc 1cchnology
23 89 2 6 7169 17 .
9 Cou | Sirau-- S H 1-ai C | ci al . Cihhcrcllin- rcgu
laic laicral rooi formaiion in Populu- ihrough inicraciion-
wiih auxin and oihcr hormonc- . | . 1hc Plani Ccll
21 22 6 7623 639 .
1 Chcn V Cai Y Liu S ci al . uaniiiaiivc Analy-i- of
Cyiokinin- in Plani- hy HPLC LSI Ion 1rap Ha-- Spcc
iromciry | . Inicgraiivc Plani Biology 21 52 1 6 7
925 932 .
11 Lockhari | A Ical P H . Prcvcniion of rcd Lighi inhihi
iion of -icm growih in ihc cucurhiiaccac hy gihhcrcllin
A4 . | . ^aiurwi--cn-chaficn 196 47 7141 142 .
12 Ha-himoio Y Yamak 1 . Comparaiivc cffcciivcnc-- of
gihhcrcllin- A1 A2 A3 and A4 wiih -pccial rcfcrcncc io
ihai of A4 | . Boi Hag 196 174 6 764 68 .
13 .
| . 28 14
17 6 72 .
14 .
I . 26 .
15 .
I . 24 .
16 .
| . 1992 1 4 6 718 22 .
17 LB / 0L . hiip 7 / / dh . food
maic . nci / pc-iicidc / rcad _ nyxz . php^ pid = 652 211
5 23 .
1 !"#$% &#(%) 211 29 5 6