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CM2859 Cryostat Microtome
CM2859 Cryostat Microtome
Operation manual
Foreword
CM2850
semi-
Cryostat
automatic
Microtome
microtome
is a
whose
design
for
tissue section.
To promise
properly
the instrument
necessary
to
read
the
to work
durable , it is
operation
manual
carefully.
Our company are not only responsible
for the repair
ability of repairing
distributors'
. To repair
the product
technology
and
constantly
products
this
manual
corresponding
will
make
of
the
updating
of technical
data
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201211190001EN
Stationary
Heat
Specimen Clamp
Extractor
Quick Freeze Shelf
Blade Holder
Peltier on freeze
shelf
Lock Lever
freezing
Area
Handwheel
Waste Container
Tool Shelf
CM 2850 Cryostat
Microtome
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201211190001EN
8 Retraction:
300 + 30J-lmls
900J-lmls+ 30J-lmls
o to 100J-lm
o to 3J-lm, in 0.5 J-lmincrements
3 to 10J-lm, in 1J-lmincrements
10 to 20J-lm, in 2J-lmincrements
20 to 100J-lm, in 5J-lmincrements
10 to 600J-lm
10 to 50J-lm, in 5J-lmincrements
50 to 100J-lm, in 10J-lmincrements
100 to 600 J-lm, in 50J-lmincrements
O~SOJ-lm,in 5J-lmincrements
8 Weight:
50lmm
Length: S05mm,
Width: 7l5mm,
Height: 1210mm,
About l30kgs
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201211190001EN
3.3 Installation
3.3.1 Unpacking
As showed in the picture, the
following
is
the
instrument
accessones:
<::>
<::>
<::>
<::>
<::>
<::>
<::>
<::>
<::>
<::>
(2)
Comer Supportl (:3)
Carton body (4)
Glass Support (5)
Comer Support 2 (6)
Comer Support 3 (7)
Handwheel (8)
Blade Holder (9)
Carton Base (1 a )
Cryostat Microtome
( 11 )
Comer Support 4 (2)
9
10
Remove the cover ( 1) and carton
body (4) , then take the following
things in order: Upholder (2) , Comer
Support 1 (3) , Comer Support 2(6) ,
Comer Support 3(7) , Comer Support
4 (12) , Handwheel (8) , Blade Holder
(9), Cryostat Microtome (11) , Glass
Support (5) . Then take out other
accessories in the inner box package to
install the instrument .
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201211190001 EN
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201211190001EN
o to 3flm,
in 0.5flm increments
in 2Jlm increments
in 5Jlm increments
is setting key .
This key is used to active setting of the section/trimming
thickness, retraction ,temperature and time.
now.
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201211190001EN
by pushing it again.
ZZ is the button to turn on/off sleep function.
/'
( As showed in left
The buttons
is to
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201211190001EN
Tighten the blade and holder and the base holder with the
four fixed poles and adjust the rotating angle as requirement
before tighten it .
The rotating angle is from 0 to 10 degree ( see the picture) ,
the user can adjust the angle in this range according to the
requirement.
15
15
.. Attention:
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201211190001 EN
r 6 UTTl20U
08t 08: 08
ls c::::J 1 N=2221im
-Press!
170023+ 5 sJ-Then
press
thicker.
When the display shows
ij]
4.4.3 Freezin
shelf temperature
Board area
Press!
TTl
lsc::J1 1 1.~"5SJ
NIIl2U
[I
CM 2850 Cryostat
Microtome
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201211190001 EN
to set minute
to enter
'-/
"
is on which means to
the item
through_; Press
Board area
then press
again
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201211190001EN
8 Feed
Press the key to adjust the specimen position to be parallel
with the blade edge for section . When the blade is far away
from specimen , press
8 Section
Just rotate right handwheel to section/trim ,and it is the
counter of slicing number.
On trimming situation, it shows 0 and could starts to count
when it turns to section situation.
Press RETRA
r T TTl
retraction setting.
tr c:::J 1 1. 1ltD
N"'2U
to enter into
23US
plate adjustment are important factors to obtain good quality section. The proper section speed
is acquired through practical experience.
some of them are mutual influence,
It needs to be worked through in practice to get better quality section under which temperature .
"'
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201211190001EN
Specimen clamp
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...
.......
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201211190001EN
Problem
Possible causes
Corrective action
No display, no
reaction to buttons
circuit
instrument is switched
on
the marks
The
section
is
section
being
followed
thickness
by
Systematically
very
try
polluted
plate
Section splinter
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201211190001EN
CM2850
No
Accessory Name
Qty
Cryostat Microtome
1 unit
Blade Holder
1 set
Handling wheel
1 pc
Specimen Clamp
10 pcs
M5 Allen Wrench
1 pc
M3Allen Wrench
1 pc
7mm Wrench
1 pc
Disposable blade
1 box
Power cord
1 pc
10
Brush
1 pc
11
Fuse
2 pcs
12
Operation Manual
1 pc
Notes
220V/8A
llOVI15A
Problem
The surface of the
section is in wave.
Possible causes
Corrective action
proper.
Increase the surrounding
humidity
It is effected by static.
proper.
If there any other troubles can not be resolved, please contact with the manufacturer.
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201211190001 EN
5.2 Maintenance
...
........
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201211190001 EN
The sectioning temperature range of most fat-free without formalin fixed is best between -13 C
and _23C .
3 ~ To obtain high quality section, please pay attention to the following aspects:
( 1) Proper cryo chamber temperature selection.
( 2) Correct section operation.
(3) careful anti-roll plate adjustment.
( 4) Sharp blade and appropriate sectioning angle
( 5) If the blade is clamped tightly.
( 6) If the specimen is clamped tightly.
Select a proper angle of the sectioning and specimen. The smaller the angle, the lower the section
compressed.
The harder the specimen, the larger the angle . If the section is still not good, please try to
00
Now there is no general rule to make sure proper of different specimen, so try to find a appropriate
angle of different specimen for good section.
4 ~ The frozen section is in contrary with paraffin section. It doesn't section long side of tissue but short
side in frozen section. Try to make contact line of tissue and blade shorter.
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201211190001EN
Press
automatically.
Section thickness
~r -e
T
m1
Progress bar
Press SECT /TRIM shortly and the line of section board area
gets thicker. When the Letter before progress bar becomes "s"
Board area
then press 0 to
can be setted by
again to exit or let
8 Trimming setting
Press SECT /TRIM shortly and the line of trimming board area
gets thicker .When the Letter before progress bar becomes "T"
Trimming thickness
~r-..
ost 08:08
1.1...J
20U 20U
Board area
__
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201211190001 EN
r T tTl
6 U 20U oat 08: 08
lst=:J1 N=2221 111l23t5eJ
l shortly
Press
"t
Ambient Temperature
.TT*
T'
6 ,U .. 20U
..-. 8.\ ..0.8.
22
"*"
:08
1 1a1.t"'J
0., .
N-2
Press
again
/".
to
CM 2850 Cryostat
Microtome
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201211190001EN
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Blade inserting
As showed in the Fig , loosen the fixed lever
(5) and push the blade(6) into blade holder(4) ,
then rotate the lever(5) to clamp the blade.
It uses blade in the picture , blade can be
divided into two types: low & high profile.
If the high profile blade is needed , twist the
four screws (7)out with 3mm Allen key, then
take out anti roll plate module (8) , twist out
the two screws (9) on base plate (10) to take
out the base plate (10).
After inserting the
blade, and refixed the anti-roll plate module(8)
with the four bolts (7) .
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201211190001 EN
11
9
3
L
As fig shows , the blade holder be composed of the following parts : blade holder base (1) ,
longitudinal movable jaw (2) , rotating movable jaw (3) , movable knife clamp(4), fixed knife
clamp (5), anti-roll plate (6) , fixed lever 1 (7) , fixed lever 2 (8) , fixed lever 3 (9) , fixed lever
4(10) , anti-roll plate lock knob (11) , anti-roll plate height adjusting knob(12) .
8 Insert the longitudinal movable jaw (2) into the track of blade holder base(1) , and rotate
lever 2(8) to lock firmly. Then adjust the handle of lever 2(8) to horizon position.
8 Adjust the rotating movable jaw(3) into longitudinal movable jaw (2) , and rotate the
lever 4(10) to lock firmly.
8 Put movable knife clamp (4) and fixed knife clamp(5) on rotating movable jaw (3) ,
rotate the lever 3 (9) to lock it firmly. Adjust the handle of lever 3 (9) to vertical position.
8 Rotate the lever 1 (7) to lock the movable knife clamp (4) and fixed knife clamp (5) .
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201211190001EN
()
real
indicator light.
defrosting
time
setting
is ready
when
the
light is on.
WAKE UP is the wake up time indicator light.
The wake up time setting when the wake up time
light is on .
immediately,
Meanwhile
the
light off.
UV is the button to tum onioffUV disinfection.
Short push this button to tum on UV disinfection ,
and tum it off by pushing it again.
03 is the button
immediately
Short push this button to tum on 03 disinfection and
tum it off by pushing it again.
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201211190001EN
4~ Operation
4.1 Control panel function & Control
All the parameters are set and displayed via control panel . After power on , use the
control panel to operate the instrument as followed:
4.1.1 Display Panel
Display area show main information
thickness,retraction
temperature
specimen
clamp
,temperat
~ : Defrosting light
SECT/TRIM: Alternative
RETRA: Retraction key
1. :
Q) :Real time
Cycle select key among
chamber
temperature
: Immediate defrosting
key
UV: UV disinfection key
03: Ozone disinfection key
key
0: Setting Key
Indicator light
~ :Defrosting time
: Key-board
lock key.
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201211190001EN
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201211190001 EN
1unit
1unit
1 pc
10 pcs
1 pc
1 pc
1 pc
1 box
1 pc
2 pcs
1 pc
1 pc
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201211190001EN
2.2Performance Index
CM2850 is a semi-automatic cryostat microtome whose specimen forward and
backward movement are controlled by motor . So it can get high quality section and easily
operated. The following is its performance :
The whole
instrument followed human engineering theory as well as artistic
appearance, made by numerical control machine.
The retraction function can prevent the specimen touch the blade sharp during lift.
It equips counter which is able to show total quantity of slice.
It equips calculator function to display the total thickness.
Artificial intelligence interface that easy to learn and operate.
Adopt UV and 03 to sterilize for 30 minutes every time .
The specimen clamp semiconductor refrigerating function is enable or disable.
Defrosting at fixed time or manually
Large freezing shelf could load 8 cassettes simultaneously.
o
o
o
o
o
2.3Technical Parameter
Environment requirements:
Normal Frequency:
Power:
Fuse:
Safe Classify:
60 Hz
:::::;1000VA
8A/15A
Classify I - Type B
__
: -35 + 2 DC
-45 + 5 DC
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-50
+ 5 DC(Working
201211190001 EN
time of
Handwheellocking mechanism
As showed in Fig, it is the lock lever .
Clockwise push the lever( I) until it lock the
handwheel at the upright position . To unlock the
handwheel , rotate counterclockwise the lever(l) .
1
As showed in Fig, the handwheel is locked.
If the lock activated , the "Lock" light will
light on display panel , while the instrument can't
run and the AUTO key is invalid.
instrument.
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201211190001EN
Contents
1"\ Safety Notes
1.1 Overview
1,."."
1,.".,1
11."."."
1,."."."
1,.,1
1,.".,1.,1.,1
1,."
II 1,."
II' 1,."
II 11."."."
1,."."
1,."
II
II
I"
Component
3
4
4
1,."
4, Operation
4.1 Operate Panel Function &Control.
4.2 Installation of Blade Holder
4.3Clamping The Specimen&Inserting
4.4 Temperature Setting
4.5 Time Setting
4.6 Section Setting
4.7 Retraction Setting
4.8 Section Reference
6, Trouble Shooting
1
2
I"
1,."
6
6
7
9
10
The Blade
10
14
16
17
18
20
20
21
22
23
24
24
HESTION
1 :::~~:7
[: :~:~
::~J